Detection and occurrence of antimicrobially resistant E. coli in groundwater on or near swine farms in eastern North Carolina

2006 ◽  
Vol 54 (3) ◽  
pp. 211-218 ◽  
Author(s):  
M.E. Anderson ◽  
M.D. Sobsey
Keyword(s):  
Growth Promotion ◽  
Enteric Bacteria ◽  
Land Application ◽  
Reference Sites ◽  
Swine Industry ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Swine Farms ◽  
Study Sites ◽  
Eastern North Carolina

The use of antibiotics for growth promotion and disease treatment by the commercial swine industry has led to high proportions of multiple antibiotic-resistant enteric bacteria being shed by these animals and concerns about the environmental spread of these bacteria. A study was conducted to quantify the extent of release of antibiotic-resistant E. coli from swine farms into groundwater. Four study sites, two swine farms and two reference sites (crop farms), with known groundwater flow paths were screened for E. coli four times over the course of one and a half years. A total of 100 biochemically-confirmed E. coli were collected from the four sites. There were statistically significantly higher E. coli levels at the two swine farm sites than at the reference sites. The bacterial isolates were tested for antibiotic resistance using a panel of 17 drugs that are typical of human and veterinary use. There were 19 and 71 E. coli isolates from swine farms #1 and #2, respectively, with most (68%) being resistant to 1–6 antimicrobials. Only one E. coli isolate from each of the reference sites showed antimicrobial resistance traits. The results of this study demonstrate that antibiotic-resistant E. coli strains are present in groundwaters of swine farms with a typical lagoon and land application system for waste management.

scholarly journals Retail Chicken Carcasses as a Reservoir of Antimicrobial- Resistant Escherichia coli

2020 ◽  
Author(s):  
Nahla Omer Eltai ◽  
Hadi M. Yassine ◽  
Sara H. Al-Hadidi ◽  
Tahra ElObied ◽  
Asmaa A. Al Thani ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Resistance ◽  
Growth Promotion ◽  
Multidrug Resistant ◽  
Chicken Meat ◽  
Resistant Bacteria ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Inappropriate Use ◽  
Esbl Producers

The dissemination of antimicrobial resistance (AMR) bacteria has been associated with the inappropriate use of antibiotics in both humans and animals and with the consumption of food contaminated with resistant bacteria. In particular, the use of antibiotics as prophylactic and growth promotion purposes in food-producing animals has rendered many of the antibiotics ineffective. The increased global prevalence of AMR poses a significant threat to the safety of the world’s food supply. Objectives: This study aims at determining the prevalence of antibiotic-resistant Escherichia coli (E. coli) isolated from local and imported retail chicken meat in Qatar. Methodology: A total of 270 whole chicken carcasses were obtained from three different hypermarket stores in Qatar. A total of 216 E. coli were isolated and subjected to antibiotic susceptibility testing against 18 relevant antibiotics using disc diffusion and micro- dilution methods. Furthermore, extended-spectrum β-lactamase (ESBL) production was determined via a double-disc synergetic test. Isolates harboring colistin resistance were confirmed using multiplex-PCR and DNA sequencing. Results: Nearly 89% (192/216) of the isolates were resistant to at least one antibiotics. In general, isolates showed relatively higher resistance to sulfamethoxazole (62%), tetracycline (59.7%), ampicillin and trimethoprim (52.3%), ciprofloxacin (47.7%), cephalothin, and colistin (31.9%). On the other hand, less resistance was recorded against amoxicillin/clavulanic acid (6%), ceftriaxone (5.1%), nitrofurantoin (4.2%) and piperacillin/tazobactam (4.2%), cefepime (2.3%), meropenem (1.4%), ertapenem (0.9%), and amikacin (0.9%). Nine isolates (4.2%) were ESBL producers. Furthermore, 63.4% were multidrug-resistant (MDR). The percentage of MDR, ESBL producers, and colistin-resistant isolates was significantly higher among local isolates compared to imported chicken samples. Conclusion: We reported a remarkably high percentage of the antibiotic-resistant E. coli in chicken meat sold at retail in Qatar. The high percentage of MDR and colistin isolates is troublesome to the food safety of raw chicken meat and the potential of antibiotic resistance spread to public health. Our findings support the need for the implementation of one health approach to address the spread of antimicrobial resistance and the need for a collaborative solution.

Antibiotic-resistance patterns of enteric bacteria of wild mammals on the Krakatau Islands and West Java, Indonesia

1988 ◽  
Vol 322 (1211) ◽  
pp. 339-353 ◽  
Keyword(s):  
Rattus Rattus ◽  
Enteric Bacteria ◽  
Antibiotic Resistant ◽  
Wild Mammals ◽  
Feral Pigs ◽  
E Coli ◽  
Resistant Species ◽  
Resistance Patterns ◽  
Faecal Bacteria ◽  
Antibiotic Resistance Patterns

Apart from feral pigs on Panjang, rats ( Rattus rattus and Rattus tiomanicus ) and bats (various families, genera and species) are the only mammals resident on the Krakatau Islands. The two species of rat occur on separate islands, R. rattus on Rakata and R. tiomanicus on Panjang and Sertung. Both occur on Java. Of the two genera of bats examined, species of Cynopterus were found on Java and all the Krakatau islands, whereas Myotis muricola muricola was detected only on Java and Rakata. The main faecal bacteria of these mammals were shown to be Escherichia coli and species of Klebsiella , Enterobacter and Citrobacter , with other bacteria (e.g. Proteus sp., Pseudomonas sp., Aeromonas sp., Serratia sp., Proteus/ Providenca sp., Morganella sp. and Streptococcus faecalis ) being present in only a minority of individuals Significant differences were noted between the faecal floras of the two rat species. E. coli was always present in R. rattus , but was less frequent in R. tiomanicus (p < 0.05), whereas species of Klebsiella and Citrobacter were less frequent in R. rattus than in R. tiomanicus (p < 0.05). Differences between the faecal floras of the two bat genera were less significant, with the four main faecal bacteria being present in each genus. However, some individual M. muricola muricola yielded S. faeclis, Morganella sp. and Proteus/Providencia sp. whereas species of Cynopterus did not ( p < 0.05). S. faecalis was detected in rats and the bat M. muricola muricola on Java, but not in mammals on the Krakataus. This may be related to the absence of humans and their domesticated animals on the Krakatau islands. Only on Java did rats carry tetracycline-resistant E. coli or tetracycline-resistant species of Klebsiella . This may be related to the widespread use of tetracycline by humans on Java, where tetracycline is available without medical supervision. Isolates of E. coli from rats on Panjang were more resistant to chloramphenicol than were E. coli isolated from rats on the other islands of the Krakatau group, and isolates of Klebsiella from bats on Panjang were more resistant to sulphamethoxazole than were Klebsiella species isolated from bats on Rakata. The reason for faecal bacteria from Panjang mammals being more antibiotic resistant than those from mammals on other islands of the group is unclear, but may be related to differences in diet and vegetation, or the presence of feral pigs on Panjang only.

Genotype, Serotype, and Antibiotic Resistance of Sorbitol-Negative Escherichia coli Isolates from Feedlot Cattle†

2009 ◽  
Vol 72 (1) ◽  
pp. 28-36 ◽  
Author(s):  
MOUSSA S. DIARRA ◽  
KARINE GIGUÈRE ◽  
FRANÇOIS MALOUIN ◽  
BRIGITTE LEFEBVRE ◽  
SUSAN BACH ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Virulence Genes ◽  
Growth Promotion ◽  
Tetracycline Resistance ◽  
Feedlot Cattle ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Arginine Dihydrolase ◽  
Stx1 Gene ◽  
Indole Production

Rectal fecal samples from 80 steers receiving Rumensin, Revalor-S, and Liquamycin alone or in combination for growth promotion and disease prevention were examined for the presence of non-O157:H7 Shiga toxin–producing Escherichia coli. All isolates were identified with the API 20E test, virulence genes were detected with a PCR assay, and antibiotic susceptibilities were determined with the Sensititre system. Of the 153 E. coli isolates recovered 126 (82.3%) were sorbitol negative. Isolates were classified into 14 biochemical E. coli groups; 51.6% were negative for arginine dihydrolase, ornithine decarboxylase, sorbitol, and saccharose reactions but positive for lysine decarboxylase, indole production, and rhamnose reactions. Twenty-one O:H serotypes were detected in the 153 E. coli isolates. The most frequent serotypes were O2:H42 (49.7% of isolates), O49:NM (13.7%), O?:H25 (9.2%), and O10:NM (7.2%). One isolate of E. coli O172:H25 and one of E. coli O157: H39 were found. The stx1 gene was found in the two E. coli O98:H25 isolates. The eaeA and e-hlyA genes were detected in 21, 14, and 10 isolates of serotypes O49:NM, O?:H25, and O10:NM, respectively, and in each isolate of serotype O156:H25 and O172:H25. Four E. coli O132:H18 isolates were multiresistant to ampicillin, chloramphenicol, kanamycin, streptomycin, and sulfisoxazole. Tetracycline resistance due to the tet(B) gene was observed in 74 of the 76 E. coli O2:H42 isolates. Except for one isolate, all tetracycline-resistant isolates were negative for the virulence genes eaeA and e-hlyA or stx1. Pulsed-field gel electrophoresis typing revealed that the tetracycline-resistant serotypes were genetically diverse. Our data illustrate that cattle are a potential source of some atypical antibiotic-resistant E. coli isolates that harbor virulence genes.

scholarly journals Effects of Zinc and Menthol-Based Diets on Co-Selection of Antibiotic Resistance among E. coli and Enterococcus spp. in Beef Cattle

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 259
Author(s):  
Sarah A. Murray ◽  
Raghavendra G. Amachawadi ◽  
Keri N. Norman ◽  
Sara D. Lawhon ◽  
Tiruvoor G. Nagaraja ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Beef Cattle ◽  
Enteric Bacteria ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Feeder Cattle ◽  
Gram Positive Bacteria ◽  
Cattle Feeding ◽  
Enterococcus Spp ◽  
The Impact

Antibiotic resistance represents a growing crisis in both human and veterinary medicine. We evaluated the use of antibiotic alternatives—heavy metals and essential oils—in beef cattle feeding, and their effects on Gram-negative and Gram-positive bacteria. In this randomized controlled field trial, we measured the impact of supplemental zinc and menthol on antibiotic resistance among commensal enteric bacteria of feeder cattle. Fecal suspensions were plated onto plain- and antibiotic-supplemented MacConkey and m-Enterococcus agar for quantification of total and antibiotic-resistant Escherichia coli and Enterococcus spp., respectively. Temporal effects on overall E. coli growth were significant (p < 0.05), and menthol was associated with decreased growth on tetracycline-supplemented agar. Zinc was associated with significant increases in growth on erythromycin-supplemented m-Enterococcus agar. Cattle fed zinc exhibited significantly higher levels of macrolide resistance among fecal enterococci isolates.

Existence and Decontamination of HVC, Infectious Enteric Bacteria and Parasites in Sewaged Soils

2014 ◽  
Vol 3 (1) ◽  
pp. 150-158 ◽  
Author(s):  
Mohey A. Hassanain ◽  
Nawal A. Hassanain ◽  
Esam A. Hobballa ◽  
Fatma H. Abd- El Zaher ◽  
Mohamed Saber M. Saber
Keyword(s):  
Soil Sample ◽  
Potassium Permanganate ◽  
Enteric Bacteria ◽  
Sewage Effluent ◽  
Calcium Hypochlorite ◽  
Sand Soil ◽  
E Coli ◽  
Surface Sample ◽  
Loamy Sand Soil ◽  
Sewage Farm

A surface sample representing a high contaminated loamy sand soil irrigated with sewage effluent since 30 years and was cultivated with artichoke was collected from Abu-Rawash sewage farm. The existence of HVC, enteric infectious bacteria and parasites in sewaged soil found to be negative for the forward and positive for the latter's. Out of the 30 samples separated from the sewaged soil sample, only 3 samples contained parasitic fauna of developed and undeveloped Ascaris (10%) and five samples contained Entamoeba coli. Results showed that the number of Ascaris eggs/gm soil was 0.017 and the number of E. coli/gm was 0.26. Decontamination of soil parasites was effective using either calcium hypochlorite or potassium permanganate. Salmonella, Vibrio and Campelobacter were detected in the high contaminated sewaged soil and survived for 120 days in the sewaged soil under all control and bioremediated treatments irrigated with either sewage effluent or water.

Relationship of antibiotic resistance to results of treatment in sepsis patients in Hue Central Hospital 2017 – 2018

2019 ◽  
pp. 48-54
Author(s):  
Duy Binh Nguyen ◽  
Trung Tien Phan ◽  
Trong Hanh Hoang ◽  
Van Tuan Mai ◽  
Xuan Chuong Tran
Keyword(s):  
Antibiotic Resistance ◽  
Bacterial Infection ◽  
Resistant Bacteria ◽  
Central Hospital ◽  
International Consensus ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Results Of Treatment ◽  
Amoxicillin Clavulanic Acid ◽  
Relationship Of

Sepsis is a serious bacterial infection. The main treatment is using antibiotics. However, the rate of antibiotic resistance is very high and this resistance is related to the outcome of treatment. Objectives: To evaluate the situation of antibiotic resistance of some isolated bacteria in sepsis patients treated at Hue Central Hospital; to evaluate the relationship of antibiotic resistance to the treatment results in patients with sepsis. Subjects and methods: prospective study of 60 sepsis patients diagnosed according to the criteria of the 3rd International Consensus-Sepsis 3 and its susceptibility patterns from April 2017 to August 2018. Results and Conclusions: The current agents of sepsis are mainly S. suis, Burkhoderiae spp. and E. coli. E. coli is resistant to cephalosporins 3rd, 4th generation and quinolone group is over 75%; resistance to imipenem 11.1%; the ESBL rate is 60%. S. suis resistant to ampicilline 11.1%; no resistance has been recorded to ceftriaxone and vancomycine. Resistance of Burkholderiae spp. to cefepime and amoxicillin/clavulanic acid was 42.9% and 55.6%, resistant to imipenem and meropenem is 20%, resistance to ceftazidime was not recorded. The deaths were mostly dued to E. coli and K. pneumoniae. The mortality for patients infected with antibiotic-resistant bacteria are higher than for sensitive groups. Key words: Sepsis, bacterial infection, antibiotics

Use of gene probes for the detection of quiescent enteric bacteria in marine and fresh waters

1995 ◽  
Vol 31 (5-6) ◽  
pp. 291-298
Author(s):  
Sally A. Anderson ◽  
Gillian D. Lewis ◽  
Michael N. Pearson
Keyword(s):  
Membrane Filtration ◽  
Enteric Bacteria ◽  
Probe Method ◽  
Detection Methods ◽  
23S Rrna ◽  
Specific Gene ◽  
Gene Probe ◽  
Selective Media ◽  
Selective Enrichment ◽  
E Coli

Specific gene probe detection methods that utilise a non-selective culturing step were tested for the ability to recognise the presence of quiescent enteric bacteria (Escherichia coli and Enterococcus faecalis ) within illuminated freshwater and seawater microcosms. An E. coli specific uidA gene probe and a 23S rRNA oligonucleotide probe for Enterococci were compared with recoveries using membrane filtration and incubation on selective media (mTEC and mE respectively). From these microcosm experiments a greater initial detection (from 4 hours to 1 day) of E. coli and Ent. faecalis using gene probe methods was observed. Additionally, a comparison of E. coli direct viable counts (DVC) in sunlight exposed microcosms with recoveries by selective media and gene probe methods revealed a large number of viable non-culturable cells. This suggests that enumeration of E. coli by a gene probe method is limited by the replication of the bacteria during the initial non-selective enrichment step. The detection of stressed Ent. faecalis by the oligonucleotide gene probe method was significantly greater than recovery on selective mE agar, indicating an Enterococci non-growth phase.

scholarly journals Multiresistant Bacteria Isolated from Intestinal Faeces of Farm Animals in Austria

Antibiotics ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 466
Author(s):  
Herbert Galler ◽  
Josefa Luxner ◽  
Christian Petternel ◽  
Franz F. Reinthaler ◽  
Juliana Habib ◽  
...  
Keyword(s):  
Meat Products ◽  
Animal Husbandry ◽  
Multidrug Resistant ◽  
Farm Animals ◽  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Multiresistant Bacteria ◽  
Vancomycin Resistant

In recent years, antibiotic-resistant bacteria with an impact on human health, such as extended spectrum β-lactamase (ESBL)-containing Enterobacteriaceae, methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE), have become more common in food. This is due to the use of antibiotics in animal husbandry, which leads to the promotion of antibiotic resistance and thus also makes food a source of such resistant bacteria. Most studies dealing with this issue usually focus on the animals or processed food products to examine the antibiotic resistant bacteria. This study investigated the intestine as another main habitat besides the skin for multiresistant bacteria. For this purpose, faeces samples were taken directly from the intestines of swine (n = 71) and broiler (n = 100) during the slaughter process and analysed. All samples were from animals fed in Austria and slaughtered in Austrian slaughterhouses for food production. The samples were examined for the presence of ESBL-producing Enterobacteriaceae, MRSA, MRCoNS and VRE. The resistance genes of the isolated bacteria were detected and sequenced by PCR. Phenotypic ESBL-producing Escherichia coli could be isolated in 10% of broiler casings (10 out of 100) and 43.6% of swine casings (31 out of 71). In line with previous studies, the results of this study showed that CTX-M-1 was the dominant ESBL produced by E. coli from swine (n = 25, 83.3%) and SHV-12 from broilers (n = 13, 81.3%). Overall, the frequency of positive samples with multidrug-resistant bacteria was lower than in most comparable studies focusing on meat products.

scholarly journals Prevalence and Characterization of Extended-Spectrum β-Lactamase-Producing Antibiotic-Resistant Escherichia coli and Klebsiella pneumoniae in Ready-to-Eat Street Foods

Antibiotics ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 850
Author(s):  
Shobha Giri ◽  
Vaishnavi Kudva ◽  
Kalidas Shetty ◽  
Veena Shetty
Keyword(s):  
Escherichia Coli ◽  
Food Safety ◽  
Klebsiella Pneumoniae ◽  
Rural Areas ◽  
Significant Risk ◽  
Microbiological Quality ◽  
Resistant Bacteria ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Extended Spectrum

As the global urban populations increase with rapid migration from rural areas, ready-to-eat (RTE) street foods are posing food safety challenges where street foods are prepared with less structured food safety guidelines in small and roadside outlets. The increased presence of extended-spectrum-β-lactamase (ESBL) producing bacteria in street foods is a significant risk for human health because of its epidemiological significance. Escherichia coli and Klebsiella pneumoniae have become important and dangerous foodborne pathogens globally for their relevance to antibiotic resistance. The present study was undertaken to evaluate the potential burden of antibiotic-resistant E. coli and K. pneumoniae contaminating RTE street foods and to assess the microbiological quality of foods in a typical emerging and growing urban suburb of India where RTE street foods are rapidly establishing with public health implications. A total of 100 RTE food samples were collected of which, 22.88% were E. coli and 27.12% K. pneumoniae. The prevalence of ESBL-producing E. coli and K. pneumoniae was 25.42%, isolated mostly from chutneys, salads, paani puri, and chicken. Antimicrobial resistance was observed towards cefepime (72.9%), imipenem (55.9%), cefotaxime (52.5%), and meropenem (16.9%) with 86.44% of the isolates with MAR index above 0.22. Among β-lactamase encoding genes, blaTEM (40.68%) was the most prevalent followed by blaCTX (32.20%) and blaSHV (10.17%). blaNDM gene was detected in 20.34% of the isolates. This study indicated that contaminated RTE street foods present health risks to consumers and there is a high potential of transferring multi-drug-resistant bacteria from foods to humans and from person to person as pathogens or as commensal residents of the human gut leading to challenges for subsequent therapeutic treatments.

Sign in / Sign up

Export Citation Format

Share Document