Calibration and field application of a molecularly imprinted membrane-passive sampler for the sampling of indicator polychlorinated biphenyls in selected aquatic environments of South Africa

2019 ◽  
Vol 79 (5) ◽  
pp. 808-819 ◽  
Author(s):  
Dennis S. Mkhize ◽  
Laura P. Quinn ◽  
Hlengilizwe Nyoni ◽  
Bhekie B. Mamba ◽  
Titus A. M. Msagati
Keyword(s):  
South Africa ◽  
Polychlorinated Biphenyls ◽  
Water Samples ◽  
Sampling Technique ◽  
Field Application ◽  
Passive Sampler ◽  
Passive Samplers ◽  
Sampling Device ◽  
Grab Sampling ◽  
Molecularly Imprinted

Abstract A passive sampling device, based on molecularly imprinted membranes (MIM), was fabricated and optimised for sampling polychlorinated biphenyls (PCBs) in aquatic ecosystems. The newly-developed passive sampler was subjected to in-situ calibration studies to determine PCB sampling rates under various conditions of water turbulence and temperature. This was carried out by exposing the passive samplers to water spiked with PCBs in a continuous-flow exposure setup. The samplers were preloaded with known concentrations of performance reference compounds (PRCs) prior to exposure. Sampling rates of seven indicator PCBs' congeners (PCBs 28, 52, 101, 118, 138, 153 and 180) ranged between 15.3 and 95.6 L/d for the different environmental conditions investigated. To determine the field suitability, the samplers were preloaded with PRCs and deployed for 10 days at the Roodeplaat and Hartbeespoort Dams, in South Africa. Water samples were taken at the end of the deployment period to compare the spot-and-grab samples to the developed samplers. PCBs 28, 101 and 138 were detected in the samplers deployed at Hartbeespoort Dam. The samplers deployed at the Roodeplaat Dam had quantifiable amounts of PCBs 28, 52, 101, 138 and 180 (0.047–0.828 ng mL−1 d−1). The sampler enhanced the detectability of PCB 52 and PCB 180, which were not detected in water samples. The field suitability trials indicated that the developed sampler could successfully be used for PCB monitoring. The sampler enhanced the detection of PCBs that would otherwise be too low to detect in samples collected through the traditional spot-and-grab sampling technique.

Pengaruh Variasi Waktu Paparan Gelombang Ultrasonik dalam Mengurangi Jumlah Bakteri coliform pada Sampel Air Sungai Kahayan

2020 ◽  
Vol 4 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Neny Kurniawati, Kerelius, Sunariyati ◽  
Luqman Hakim, Dyah Ayu Pramoda Wardani, Widya Krestina ◽  
Dwi Tyas Setiawan, Ferry Purwanto, Diah K. Fatmala
Keyword(s):  
Water Treatment ◽  
River Water ◽  
Ultrasonic Wave ◽  
Exposure Time ◽  
Water Samples ◽  
Sampling Technique ◽  
Wave Exposure ◽  
Test Results ◽  
Time Data ◽  
Grab Sampling

Abstrak – Penelitian ini bertujuan untuk mengkaji pengaruh waktu paparan gelombang ultrasonik terhadap pengurangan jumlah koloni bakteri coliform pada sampel air sungai Kahayan. Pengambilan sampel air sungai dilakukan dengan teknik Grab Sampling menggunakan alat Kemmerer Sampler. Sampel air yang didapatkan diberikan paparan gelombang ultrasonik secara langsung, tanpa merubah kondisi lingkungan awal. Waktu paparan divariasikan pada 1 jam, 2 jam, 3 jam, 4 jam, dan 5 jam dengan frekuensi 40 kHz untuk memperoleh data waktu optimum. Uji coliform dilakukan dengan metode MPN, dengan tahapan uji pendugaan, uji penegasan, dan perhitungan koloni. Hasil uji MPN 24 jam setelah paparan menunjukkan bahwa penggunaan ultrasonik sebagai antibateri dapat optimum ketika diberikan paparan dengan waktu 3 jam, dengan efisiensi  96%. Kata kunci: antibakteri, coliform, ultrasonik, water treatment, sungai Kahayan  Abstract – This study aims to examine the effect of ultrasonic wave exposure time on reducing the number of coliform bacterial colonies in the Kahayan river water samples. River water sampling is done using the Grab Sampling technique using the Kemmerer Sampler tool. The water samples obtained were given direct ultrasonic wave exposure, without changing the initial environmental conditions. The exposure time is varied in 1 hour, 2 hours, 3 hours, 4 hours and 5 hours with a frequency of 40 kHz to obtain optimum time data. Coliform test was carried out by the MPN method, with the stages of the estimation test, affirmation test, and colony calculation. The MPN test results 24 hours after exposure showed that the use of ultrasonic as an antibody can be optimum when given exposure with a time of 3 hours, with an efficiency of 96%.Keywords : antibakterial, coliform, ultrasonic, water treatment, Kahayan river

scholarly journals Analisis Pencemaran Kromium (VI) berdasarkan Kadar Chemical Oxygen Demand (COD) pada Hulu Sungai Citarum di Kecamatan Majalaya Kabupaten Bandung Provinsi Jawa Barat 2018

2020 ◽  
Vol 19 (2) ◽  
pp. 144-151
Author(s):  
Arif Sumantri ◽  
Rifqi Zakiya Rahmani
Keyword(s):  
Chemical Oxygen Demand ◽  
Water Samples ◽  
Oxygen Demand ◽  
Sampling Technique ◽  
Quality Standard ◽  
Grab Sampling ◽  
Purposive Sampling ◽  
River Waters ◽  
Chromium Vi ◽  
Sample Testing

Latar Belakang: Limbah industri tekstil menghasilkan limbah logam berat kromium (VI)) untuk digunakan dalam proses produksi. Kromium (VI) yang masuk ke dalam air bisa menjadi masalah kesehatan baik jangka pendek maupun panjang. Salah satu wilayah industri yang banyak berkembang terdapat di sektor hulu Sungai Citarum terdapat di Kecamatan Majalaya, Kabupaten Bandung sebagai kawasan zona industri.Terdapat sekitar 1500 industri dengan potensi jumlah limbah yang dibuang mencapai 2.800 ton per hari  yang sekaligus sebagai sumber pencemaran paling dominan.Metode: Instrumen penelitian ini adalah observasi dan pengujian sampel di laboratorium.Sampel diambil selama 3 hari berturut-turut di 7 stasiun di setiap lokasi. Variabel yang diuji yaitu, kromium (VI), COD, DO, dan pH. Metode pengambilan yang digunakan adalah grab sampel dengan teknik purposive sampling. Waktu untuk mengambil sampel air dilakukan pada malam hari. Sampel air diawetkan dengan cara pengasaman dan pendinginan sesuai dengan SNI 6989.57: 2008. Hasil penelitian ini dianalisis dengan membandingkan dengan Peraturan Pemerintah No.82 Tahun 2001.Hasil: Hasil penelitian menunjukan konsentrasi kromium (VI)di stasiun A1 adalah 0,042 mg/l, stasiun A2 0,44 mg/l, di stasiun A3 adalah 0,075 mg/l dan stasiun A4 adalah 0,093 mg/l. Selain itu, di stasiun B1, B2, dan B3 konsentrasi di bawah 0,04 mg/l (batas alat minimum). Kadar kromium (VI) A3 dan A4 tidak memenuhi persyaratan menurut PP No.82 tahun 2001 standar kualitas kromium (VI)di badan air kelas II adalah 0,05 mg/l.Simpulan: Air Sungai Citarum di Kabupaten Majalaya telah tercemar oleh kromium (VI). Dari hasil, kromium (VI)telah melebihi perairan sungai dari stasiun A3 dan A4 dengan kadar 0,075 mg/l dan 0,093 mg/l. ABSTRACTTitle: Analysis of Chromium IV pollution based on Chemical Oxygen Demand at Citarum River, Majalaya District, Bandung Regency, West JavaBackground: Textile industrial waste produces heavy metal waste chromium (VI) for use in the production process. Chromium (VI) that gets into water can be a health problem both in the short and long term. One of the most developed industrial areas is in the upstream sector of the Citarum River in Majalaya District, Bandung Regency as an industrial zone. There are around 1500 industries with the potential for the amount of waste disposed of up to 2,800 tons per day which is also the most dominant source of pollution.Methods: The research instrument was observation and sample testing in the laboratory. Samples were taken for 3 consecutive days at 7 stations in each location. The variables tested were chromium (VI), COD, DO, and pH. The method used is grab sampling with purposive sampling technique. Time to take water samples is done at night. The water samples were preserved by means of acidification (HNO3 and H2SO4) and cooling according to SNI 6989.57: 2008. The results of this study were analyzed by comparing with PP No.82 of 2001.Results: The results showed that the chromium (VI) concentration at station A1 was 0.042 mg / l, station A2 was 0.44 mg / l, at station A3 was 0.075 mg / l and station A4 was 0.093 mg / l. In addition, at stations B1, B2, and B3 the concentration was below 0.04 mg / l (minimum equipment limit). Chromium (VI) A3 and A4 levels do not meet the requirements according to PP No. 82 of 2001 the quality standard for chromium (VI) in class II water bodies is 0.05 mg / l.Conclusion: Citarum River water in Majalaya Regency has been polluted by chromium (VI). From the results, chromium (VI) has exceeded the river waters of stations A3 and A4 with levels of 0.075 mg / l and 0.093 mg / l. 

Passive Sampling of Polycyclic Aromatic Hydrocarbons in Johor Strait by Sol–gel Polydimethylsiloxane

2013 ◽  
Vol 62 (3) ◽  
Author(s):  
Cian Ying Tung ◽  
Razali Ismail ◽  
Jafariah Jaafar
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
Passive Sampling ◽  
Sol Gel ◽  
Sampling Technique ◽  
Passive Sampler ◽  
Grab Sampling ◽  
Exposure System ◽  
Polycyclic Aromatic ◽  
Johor Strait

Passive sampling technique is an alternative technique in the monitoring of organic pollutants in aqueous environment. This technique is able to overcome several drawbacks of the conventional grab sampling technique. In this study, passive sampler consists of glass tubes coated with sol–gel polydimethylsiloxane (PDMS) were fabricated in the laboratory. The passive samplers were used to study the level and distribution of 15 polycyclic aromatic hydrocarbons (PAHs) in Johor Strait. Performance of the passive sampler was tested in the laboratory with an exposure system that has similar flow rate, temperature and water salinity with Johor Strait. Then, the passive sampler was deployed to five sampling stations located in Johor Strait for 7 and 30 days to accumulate PAHs in seawater. High performance liquid chromatography with fluorescence detector was used to analyze PAHs in the samples. All PAHs spiked in the exposure system were found to accumulate in the PDMS coating. Mass of 15 PAHs extracted from the passive sampler after deployment to Johor Strait was in the range of 0.372–1.131 ng/PDMS glass tube. Findings obtained from passive sampling method and seawater analysis were comparable. Passive sampling technique is low cost, simple and less solvent consumption.

A Sensitive and Selective Electrochemical Sensor for Sulfadimethoxine Based on Electropolymerized Molecularly Imprinted Poly (o-aminophenol) Film

2020 ◽  
Vol 16 (4) ◽  
pp. 413-420 ◽  
Author(s):  
Youyuan Peng ◽  
Qiaolan Ji
Keyword(s):  
Electrochemical Sensor ◽  
Polymer Film ◽  
Water Samples ◽  
Differential Pulse ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Long Term Stability ◽  
Multi Walled Carbon Nanotubes ◽  
Aquaculture Water ◽  
Walled Carbon Nanotubes

Background: As a broad-spectrum antibiotic of the sulfonamide family, Sulfadimethoxine (SDM) has been widely utilized for therapeutic and growth-promoting purposes in animals. However, the use of SDM can cause residual problems. Even a low concentration of SDM in the aquatic system can exert toxic effects on target organisms and green algae. Therefore, the quantitation of SDM residues has become an important task. Methods: The present work describes the development of a sensitive and selective electrochemical sensor for sulfadimethoxine based on molecularly imprinted poly(o-aminophenol) film. The molecular imprinted polymer film was fabricated by electropolymerizing o-aminophenol in the presence of SDM after depositing carboxylfunctionalized multi-walled carbon nanotubes onto a glassy carbon electrode surface. SDM can be quickly removed by electrochemical methods. The imprinted polymer film was characterized by cyclic voltammetry, differential pulse voltammetry and scanning electron microscopy. Results: Under the selected optimal conditions, the molecularly imprinted sensor shows a linear range from 1.0 × 10-7 to 2.0 × 10-5 mol L-1 for SDM, with a detection limit of 4.0 × 10-8 mol L-1. The sensor was applied to the determination of SDM in aquaculture water samples successfully, with the recoveries ranging from 95% to 106%. Conclusion: The proposed sensor exhibited a high degree of selectivity for SDM in comparison to other structurally similar molecules, along with long-term stability, good reproducibility and excellent regeneration capacity. The sensor may offer a feasible strategy for the analysis of SDM in aquaculture water samples.

scholarly journals Development of Surface Molecularly Imprinted Polymers as Dispersive Solid Phase Extraction Coupled with HPLC Method for the Removal and Detection of Griseofulvin in Surface Water

2019 ◽  
Vol 17 (1) ◽  
pp. 134 ◽  
Author(s):  
Kamran Bashir ◽  
Zhimin Luo ◽  
Guoning Chen ◽  
Hua Shu ◽  
Xia Cui ◽  
...  
Keyword(s):  
Surface Water ◽  
Solid Phase Extraction ◽  
Molecularly Imprinted Polymers ◽  
Water Samples ◽  
Solid Phase ◽  
Phase Extraction ◽  
Dispersive Solid Phase Extraction ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Surface Water Samples

Griseofulvin (GSF) is clinically employed to treat fungal infections in humans and animals. GSF was detected in surface waters as a pharmaceutical pollutant. GSF detection as an anthropogenic pollutant is considered as a possible source of drug resistance and risk factor in ecosystem. To address this concern, a new extraction and enrichment method was developed. GSF-surface molecularly imprinted polymers (GSF-SMIPs) were prepared and applied as solid phase extraction (SPE) sorbent. A dispersive solid phase extraction (DSPE) method was designed and combined with HPLC for the analysis of GSF in surface water samples. The performance of GSF-SMIPs was assessed for its potential to remove GSF from water samples. The factors affecting the removal efficiency such as sample pH and ionic strength were investigated and optimized. The DSPE conditions such as the amount of GSF-SMIPs, the extraction time, the type and volume of desorption solvents were also optimized. The established method is linear over the range of 0.1–100 µg/mL. The limits of detection and quantification were 0.01 and 0.03 µg/mL respectively. Good recoveries (91.6–98.8%) were achieved after DSPE. The intra-day and inter-day relative standard deviations were 0.8 and 4.3% respectively. The SMIPs demonstrated good removal efficiency (91.6%) as compared to powder activated carbon (67.7%). Moreover, the SMIPs can be reused 10 times for water samples. This is an additional advantage over single-use activated carbon and other commercial sorbents. This study provides a specific and sensitive method for the selective extraction and detection of GSF in surface water samples.

scholarly journals Characterisation of mobile colistin resistance genes (mcr-3 and mcr-5) in river and storm water in regions of the Western Cape of South Africa

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Yolandi Snyman ◽  
Andrew C. Whitelaw ◽  
Jo M. Barnes ◽  
Motlatji R. B. Maloba ◽  
Mae Newton-Foot
Keyword(s):  
South Africa ◽  
Water Samples ◽  
Pathogenic Bacteria ◽  
Multidrug Resistant ◽  
Water Sources ◽  
Storm Water ◽  
Gene Variants ◽  
Western Cape ◽  
Colistin Resistance ◽  
Three Rivers

Abstract Background Colistin is regarded as a last-resort antimicrobial against multi-drug resistant Gram-negative bacteria (GNB), therefore the dissemination of colistin resistance in the environment is of great concern. Horizontal transfer of mobile colistin resistance (mcr) genes to potential pathogens poses a serious problem. This study aimed to describe the presence of colistin resistant GNB and mcr genes in river and storm water in regions of the Western Cape. Methods Water samples were collected from three rivers during May 2019 and January 2020 and two storm water samples were collected in November 2019. Colistin resistant GNB were cultured on MacConkey agar containing colistin and identified by MALDI-TOF. Colistin resistance was confirmed using broth microdilution (BMD). mcr-1-5 genes were detected by PCR performed directly on the water samples and on the colistin resistant isolates. mcr functionality was assessed by BMD after cloning the mcr genes into pET-48b(+) and expression in SHuffle T7 E. coli. Results mcr-5.1 and various mcr-3 gene variants were detected in the Plankenburg-, Eerste- and Berg rivers and in storm water from Muizenberg, and only mcr-5.1 was detected in storm water from Fish Hoek. Colistin resistant GNB were isolated from all of the water sources. Aeromonas spp. were the most common colistin resistant organisms detected in the water sources; 25% (6/24) of colistin resistant Aeromonas spp. isolated from the Berg river contained novel mcr-3 variants; mcr-3.33 (n = 1), mcr-3.34 (n = 1) mcr-3.35 (n = 1) mcr-3.36 (n = 2) and mcr-3.37 (n = 1), which were confirmed to confer colistin resistance. Conclusions The mcr-5.1 and mcr-3 colistin resistance gene variants were present in widely dispersed water sources in regions of the Western Cape. The mcr genes were only detected in water sampled downstream of and alongside communities, suggesting that their presence is driven by human influence/contamination. This is the first documentation of mcr-3 and mcr-5 gene variants in any setting in South Africa. Spill-over of these genes to communities could result in horizontal gene transfer to pathogenic bacteria, exacerbating the challenge of controlling multidrug resistant GNB infections.

scholarly journals Alcohol Abuse as a Militating Factor against Quality of Life for Migrants’ Youth Population in Selected Provinces of South Africa

2021 ◽  
Vol 10 ◽  
pp. 1196-1201
Author(s):  
F.K. Matlakala ◽  
◽  
J.C. Makhubele ◽  
D.T. Masilo ◽  
M.M. Kwakwa ◽  
...  
Keyword(s):  
Quality Of Life ◽  
South Africa ◽  
Alcohol Abuse ◽  
Sampling Technique ◽  
Alcohol Outlets ◽  
Job Opportunities ◽  
Study Population ◽  
Youth Population

Migrants’ youth are seen as one of the vulnerable populations in South Africa. This is largely due to the fact that they are seen as people who come to take job opportunities of the youth in the host country. In order to cope with their fear and stress, migrants indulge in binge consumption of alcohol. It is in light of that that in this paper researchers aimed to accentuate alcohol abuse as a militating factor against the quality of life for migrants’ youth population in selected provinces of South Africa. The study adopted qualitative approach and case study design to highlight how alcohol is seen as a militating factor against quality of life. The study population was drawn from three provinces in South Africa using convenient sampling technique to sample three participants. Moreover, the data was collected telephonically in three selected provinces and analysed thematically. The findings indicate that due to the accessibility, availability, affordability and stress migrants’ youth indulge in binge consumption. Thus, researchers recommend that policymakers should make guidelines that will restrict mushrooming of alcohol outlets – be regulation to prohibit overcrowding of outlets in selected provinces of South Africa.

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