Uterine Wound Healing: A Complex Process Mediated by Proteins and Peptides

We have developed an intestinal wound model that includes surgical construction of an ileo-cecal patch to study the complex process of intestinal wound healing. This allows approximation of ileal mucosa to the cecal serosa and facilitates regeneration of ileal mucosa onto the serosal surface of the cecum. The regeneration of ileal mucosa can then be evaluated at different times. The wound model also allows us to determine the rate of intestinal regeneration for a known size of intestinal wound and can be compared in different situations (e.g. with and without EGF and Peyer’s patches).At the light microscopic level it appeared that epithelial cells involved in regeneration of ileal mucosa originated from the enlarged crypts adjacent to the intestinal wound and migrated in an orderly fashion onto the serosal surface of the cecum. The migrating epithelial cells later formed crypts and villi by the process of invagination and evagination respectively. There were also signs of proliferation of smooth muscles underneath the migratory epithelial cells.

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Composite Ferroelectric Membranes Based on Vinylidene Fluoride-Tetrafluoroethylene Copolymer and Polyvinylpyrrolidone for Wound Healing

Membranes ◽

10.3390/membranes11010021 ◽

2020 ◽

Vol 11 (1) ◽

pp. 21

Author(s):

Tamara S. Tverdokhlebova ◽

Ludmila S. Antipina ◽

Valeriya L. Kudryavtseva ◽

Ksenia S. Stankevich ◽

Ilya M. Kolesnik ◽

...

Keyword(s):

Wound Healing ◽

Vinylidene Fluoride ◽

Ferroelectric Properties ◽

Composite Membranes ◽

Modern Medicine ◽

In Vivo Studies ◽

Cytotoxic Effects ◽

Complex Process ◽

Purulent Wounds

Wound healing is a complex process and an ongoing challenge for modern medicine. Herein, we present the results of study of structure and properties of ferroelectric composite polymer membranes for wound healing. Membranes were fabricated by electrospinning from a solution of vinylidene fluoride/tetrafluoroethylene copolymer (VDF–TeFE) and polyvinylpyrrolidone (PVP) in dimethylformamide (DMF). The effects of the PVP content on the viscosity and conductivity of the spinning solution, DMF concentration, chemical composition, crystal structure, and conformation of VDF–TeFE macromolecules in the fabricated materials were studied. It was found that as PVP amount increased, the viscosity and conductivity of the spinning solutions decreased, resulting in thinner fibers. Using FTIR and XRD methods, it was shown that if the PVP content was lower than 50 wt %, the VDF–TeFE copolymer adopted a flat zigzag conformation (TTT conformation) and crystalline phases with ferroelectric properties were formed. Gas chromatography results indicated that an increase in the PVP concentration led to a higher residual amount of DMF in the material, causing cytotoxic effects on 3T3L1 fibroblasts. In vivo studies demonstrated that compared to classical gauze dressings impregnated with a solution of an antibacterial agent, ferroelectric composite membranes with 15 wt % PVP provided better conditions for the healing of purulent wounds.

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Updates in Diabetic Wound Healing, Inflammation, and Scarring

Seminars in Plastic Surgery ◽

10.1055/s-0041-1731460 ◽

2021 ◽

Author(s):

Nina Dasari ◽

Austin Jiang ◽

Anna Skochdopole ◽

Jayer Chung ◽

Edward Reece ◽

...

Keyword(s):

Wound Healing ◽

Disease Process ◽

Wound Dehiscence ◽

Diabetic Patients ◽

Wound Infections ◽

Diabetic Wound ◽

Complex Process ◽

Diabetic Wound Healing ◽

Almost All ◽

Diabetic Wounds

AbstractDiabetic patients can sustain wounds either as a sequelae of their disease process or postoperatively. Wound healing is a complex process that proceeds through phases of inflammation, proliferation, and remodeling. Diabetes results in several pathological changes that impair almost all of these healing processes. Diabetic wounds are often characterized by excessive inflammation and reduced angiogenesis. Due to these changes, diabetic patients are at a higher risk for postoperative wound healing complications. There is significant evidence in the literature that diabetic patients are at a higher risk for increased wound infections, wound dehiscence, and pathological scarring. Factors such as nutritional status and glycemic control also significantly influence diabetic wound outcomes. There are a variety of treatments available for addressing diabetic wounds.

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Acceleration of Palatal Wound Healing in Smad3-deficient Mice

Journal of Dental Research ◽

10.1177/0022034509341798 ◽

2009 ◽

Vol 88 (8) ◽

pp. 757-761 ◽

Cited By ~ 18

Author(s):

K. Jinno ◽

T. Takahashi ◽

K. Tsuchida ◽

E. Tanaka ◽

K. Moriyama

Keyword(s):

Wound Healing ◽

Transforming Growth Factor ◽

Smooth Muscle Actin ◽

Monocyte Chemoattractant Protein 1 ◽

Inflammatory Protein ◽

Complex Process ◽

Dermal Cells ◽

Macrophage Inflammatory Protein ◽

Tgf Β1

Wound healing is a well-orchestrated complex process leading to the repair of injured tissues. It is suggested that transforming growth factor (TGF)-β/Smad3 signaling is involved in wound healing. The purpose of this study was to investigate the role of TGF-β/Smad3 signaling in palatal wound healing in Smad3-deficient (Smad3−/−) mice. Histological examination showed that wound closure was accelerated by the proliferation of epithelium and dermal cells in Smad3−/− mice compared with wild-type (WT) mice. Macrophage/monocyte infiltration at wounded regions in Smad3−/− mice was decreased in parallel with the diminished production of TGF-β1, monocyte chemoattractant protein-1, and macrophage inflammatory protein-1α compared with WT mice. Fibrocytes, expressing hematopoietic surface marker and fibroblast products, were recruited and produced α-smooth-muscle actin in WT mice, but were not observed in Smad3−/− mice. These results suggest that TGF-β/Smad3 signaling may play an important role in the regulation of palatal wound healing.

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Rapid epithelialisation of fetal wounds is associated with the early deposition of tenascin

Journal of Cell Science ◽

10.1242/jcs.99.3.583 ◽

1991 ◽

Vol 99 (3) ◽

pp. 583-586 ◽

Cited By ~ 5

Author(s):

D.J. Whitby ◽

M.T. Longaker ◽

M.R. Harrison ◽

N.S. Adzick ◽

M.W. Ferguson

Keyword(s):

Wound Healing ◽

Cell Migration ◽

Temporal Distribution ◽

Cell Types ◽

Skin Wound ◽

Skin Wound Healing ◽

Early Appearance ◽

Complex Process ◽

Healing Wounds ◽

Fetal Wound

Wound healing is a complex process involving the interaction of many cell types with the extracellular matrix (ECM). Fetal skin wound healing differs from that in the adult in that it occurs rapidly and without scar formation. The mechanisms underlying these differing processes may be related to the fetal environment, the stage of differentiation of the fetal cells or the ECM deposited in the wound. The spatial and temporal distribution of two components of the ECM, fibronectin and tenascin, were studied by immunostaining of cryosections from trunk wounds of fetal and adult sheep. Epithelialisation was complete earlier in the fetal wound than in the adult. The distribution of fibronectin was similar in fetal and adult wounds but tenascin was present earlier in the fetal wound. Fibronectin has several roles in wound healing including acting as a substratum for cell migration and as a mediator of cell adhesion through cell surface integrins. The attachment of fibroblasts to fibronectin is inhibited by tenascin and during development the appearance of tenascin in the ECM of migratory pathways correlates with the initiation of cell migration. Similarly, the appearance of tenascin in healing wounds may initiate cell migration. Tenascin was present in these wounds prior to cell migration and the rapid epithelialisation of fetal wounds may be due to the early appearance of tenascin in the wound.

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Topical Application of Virus-Derived Immunomodulating Proteins and Peptides to Promote Wound Healing in Mouse Models

Methods in Molecular Biology - Viruses as Therapeutics ◽

10.1007/978-1-0716-1012-1_12 ◽

2020 ◽

pp. 217-226

Author(s):

Liqiang Zhang ◽

Jordan R. Yaron ◽

Qiuyun Guo ◽

Jacquelyn Kilbourne ◽

Enkidia A. Awo ◽

...

Keyword(s):

Wound Healing ◽

Mouse Models ◽

Topical Application ◽

Promote Wound Healing ◽

Proteins And Peptides

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Activated Protein C in Cutaneous Wound Healing: From Bench to Bedside

International Journal of Molecular Sciences ◽

10.3390/ijms20040903 ◽

2019 ◽

Vol 20 (4) ◽

pp. 903 ◽

Cited By ~ 5

Author(s):

Ruilong Zhao ◽

Haiyan Lin ◽

Lara Bereza-Malcolm ◽

Elizabeth Clarke ◽

Christopher Jackson ◽

...

Keyword(s):

Wound Healing ◽

Clinical Studies ◽

Protein C ◽

Activated Protein C ◽

Epithelial Barrier ◽

Cutaneous Wound Healing ◽

Cutaneous Wound ◽

Beneficial Effects ◽

The Past ◽

Complex Process

Independent of its well-known anticoagulation effects, activated protein C (APC) exhibits pleiotropic cytoprotective properties. These include anti-inflammatory actions, anti-apoptosis, and endothelial and epithelial barrier stabilisation. Such beneficial effects have made APC an attractive target of research in a plethora of physiological and pathophysiological processes. Of note, the past decade or so has seen the emergence of its roles in cutaneous wound healing—a complex process involving inflammation, proliferation and remodelling. This review will highlight APC’s functions and mechanisms, and detail its pre-clinical and clinical studies on cutaneous wound healing.

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Preparation and Comparison of Effects of Different Herbal Oil Ointments as Wound-Healing Agents

Cells Tissues Organs ◽

10.1159/000503624 ◽

2019 ◽

Vol 207 (3-4) ◽

pp. 177-186 ◽

Cited By ~ 1

Author(s):

Alireza Valizadeh ◽

Mahdieh Shirzad ◽

Mohammad Reza Pourmand ◽

Maryam Farahmandfar ◽

Hassan Sereshti ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Dorsal Surface ◽

Inflammatory Cells ◽

Hair Follicles ◽

Sesame Oil ◽

Full Thickness ◽

Olive Oils ◽

Complex Process ◽

And Control

Wound healing is a complex process and some agents have been reported to accelerate it. The aim of this study was to evaluate the healing effect of Eucerin-based ointments of lemon, sesame and olive oils on infected full-thickness wounds in rats. Wounds were created on the dorsal surface of Male Albino Wistar rats (n = 12). Wounds were treated with an Eucerin-based ointment containing either of lemon, sesame or olive oils (33% w/w) twice a day for 14 days. Histopathology results showed that contraction of wounds treated with lemon and sesame oils was higher than in the olive oil and control groups on days 10 and 14. In the lemon- and sesame-oil treated groups, on day 14, 50% of rat lesions were completely healed. Total number of inflammatory cells in lemon oil treatment group was significantly smaller than that of others on day 14 (p < 0.001). Also, thickness of the epidermal layer and rejuvenation of the hair follicles and other skin appendages was normal in lemon and sesame oil treated groups. The lemon and sesame oil ointments accelerated the healing process of wounds in macroscopic, morphological and morphmetrical analyses. Therefore, lemon and sesame oil ointments could be considered as alternative dressings for infected full-thickness wounds because of improved wound healing characteristics.

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Epidermal Vascular Endothelial Growth Factor Improved Wound Healing of Patients with Diabetic Foot Ulcer

Advanced Science Letters ◽

10.1166/asl.2018.12653 ◽

2018 ◽

Vol 24 (8) ◽

pp. 6136-6139

Author(s):

Maria Francisca Ham ◽

Pradana Soewondo ◽

Saphora Dien ◽

Kusmardi Kusmardi ◽

Mpu Kanoko

Keyword(s):

Wound Healing ◽

Growth Factor ◽

Diabetic Foot ◽

Standard Procedure ◽

Vascular Complications ◽

Foot Ulcer ◽

Diabetic Foot Ulcer ◽

Complex Process ◽

Proximal Site ◽

Endothelial Growth Factor

Diabetic foot ulcer (DFU) is a kind of chronic vascular complications in diabetes. Wound healing in DFU is a complex process where reduced proangiogenic growth factor is an important factor. We studied angiogenesis factors which may contribute to the healing of DFU. Twenty five type 2 diabetes patients with acute DFU were treated with hospital standard procedure and closely observed for 1 month. Biopsies from wound edges were subjected to histopathology assessment and immunohistochemical staining of CD34, bFGF and VEGF. All examination was performed two times consecutively. We observed that granulation tissues developed faster at proximal site of the foot. Weak tissue VEGF was expressed before treatment. Interestingly, VEGF expression in epidermis increased significantly after patients received treatment. Increased VEGF is consistent with increased number of CD34 positive endothelial cells. These data suggest that epidermal VEGF is an important angiogenesis factor and may improve healing in acute DFU receiving standard treatment.

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