Exploring the potential of molecular spectroscopy for the detection of post-translational modifications of a stressed biopharmaceutical protein

2021 ◽  
Vol 22 ◽  
Author(s):  
Marco Pinto Corujo ◽  
Vivian Lindo ◽  
Nikola Chmel ◽  
Alison Rodger
Keyword(s):  
Messenger Rna ◽  
Molecular Spectroscopy ◽  
Order Structure ◽  
Tertiary Structures ◽  
Post Translational Modifications ◽  
Ir And Raman Spectroscopies ◽  
Vibrational Spectroscopies ◽  
Ir Analysis ◽  
Ir And Raman ◽  
Raman And Ir Spectra

Background: Proteins are biomolecules that consist of sequences of amino acids (primary structure) which can further interact and cause the backbone to fold into more complex structures (secondary and tertiary structures). Any chemical alterations of the molecules after the translation of the messenger RNA code into a protein primary sequence are known as post-translational modifications (PTMs). PTMs may affect the protein’s functionality; thus it is necessary to identify them. PTMs of particular interest to the pharmaceutical industry include deamidation, oxidation, deglycosylation and isomerization, which may occur due to environmental stressors. However, they have proved challenging to identify quickly. Electronic and vibrational spectroscopies have proved valuable tools for studying higher-order structure and stability of proteins. Materials & Methods: In this work, circular dichroism (CD), infrared absorbance (IR) and Raman spectroscopies were applied to characterize antibody (mAb NIP 228) PTMs as a result of different stressors. Mass spectrometry was used to confirm the identity of modifications including the targeted ones. Room temperature CD showed that the secondary structure was the same after all treatments, and temperature-controlled CD showed how protein stability was affected by modifications. Both Raman and IR analysis detected small differences between the reference and deglycosylated proteins, and clearly indicated the presence of other PTMs. Conclusion: This work required some novel computational approaches to pre–process Raman and IR spectra and a review of the band assignments for proteins existing in the literature.

scholarly journals Analysis of the Quintilii’s Villa Bronzes by Spectroscopy Techniques

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Fabio Stranges ◽  
Mauro La Russa ◽  
Antonino Oliva ◽  
Giuliana Galli
Keyword(s):  
Diagnostic Tests ◽  
Electron Spectroscopy ◽  
Morphological Analysis ◽  
Alloy Composition ◽  
Molecular Spectroscopy ◽  
Electronic Spectroscopy ◽  
Diagnostic Techniques ◽  
Ir And Raman Spectroscopies ◽  
Ir And Raman ◽  
Alloy Analysis

The aim of this work is the characterization, with different diagnostic tests, of three fragments of bronze artefacts recovered from the Villa of the Quintilii (located in the south of Rome). In particular, the sample alloys were investigated by different chemical and morphological analysis. Firstly, an analysis of the alloy, implemented through the electronic spectroscopy, was taken to discriminate the bronze morphology and its elemental composition. Subsequently, a surface analysis was realized by molecular spectroscopy to identify the alteration patinas on surfaces (such as bronze disease). Two diagnostic techniques are used for the alloy analysis: scanning electron microscopy (SEM) connected to the EDX spectroscopy (to study the morphology and alloy composition) and Auger electron spectroscopy (AES) (to identify the oxidation state of each element). Moreover, for the study of surface patinas, IR and Raman spectroscopies were implemented. All studies were performed on the “as received” samples, covered by a thin layer of excavated soil and on samples processed in an aqueous solution of sulphuric acid (10%), to remove patinas and alterations.

scholarly journals Sonochemical-assisted magnesium borate synthesis from different boron sources

2017 ◽  
Vol 19 (1) ◽  
pp. 81-88 ◽  
Author(s):  
Meral Yildirim ◽  
Azmi Seyhun Kipcak ◽  
Emek Moroydor Derun
Keyword(s):  
High Efficiency ◽  
Synthesis Method ◽  
Spectroscopic Studies ◽  
X Ray Diffraction ◽  
Magnesium Borate ◽  
Ir And Raman Spectroscopies ◽  
Ft Ir ◽  
Surface Morphologies ◽  
Ir And Raman ◽  
Good Agreement

Abstract In this study, sonochemical-assisted magnesium borate synthesis is studied from different boron sources. Various reaction parameters are successfully applied by a simple and green method. X-ray diffraction (XRD), Fourier transform infrared (FT-IR) and Raman spectroscopies are used to characterize the synthesized magnesium borates on the other hand surface morphologies are investigated by using scanning electron microscope (SEM). The XRD analyses showed that the products were admontite [MgO(B2O3)3 · 7(H2O)] with JCPDS (Joint Committee on Powder Diffraction Standards) no. of 01-076-0540 and mcallisterite [Mg2(B6O7(OH)6)2 · 9(H2O)] with JCPDS no. of 01-070-1902. The results that found in the spectroscopic studies were in a good agreement with characteristic magnesium borate bands in both regions of infra-red and visible. According to SEM results, obtained borates were in micro and sub-micro scales. By the use of ultrasonication, reaction yields were found between 84.2 and 97.9%. As a result, it is concluded that the sonochemical approach is a practicable synthesis method to get high efficiency and high crystallinity in the synthesis magnesium borate compounds.

scholarly journals eIF-Three to Tango: emerging functions of translation initiation factor eIF3 in protein synthesis and disease

2020 ◽  
Vol 12 (6) ◽  
pp. 403-409 ◽  
Author(s):  
Dieter A Wolf ◽  
Yingying Lin ◽  
Haoran Duan ◽  
Yabin Cheng
Keyword(s):  
Protein Synthesis ◽  
Messenger Rna ◽  
Protein Production ◽  
Mrna Translation ◽  
Translation Initiation Factor ◽  
Initiation Factor ◽  
Protein Homeostasis ◽  
Subcellular Targeting ◽  
Post Translational Modifications ◽  
The Past

Abstract Studies over the past three years have substantially expanded the involvements of eukaryotic initiation factor 3 (eIF3) in messenger RNA (mRNA) translation. It now appears that this multi-subunit complex is involved in every possible form of mRNA translation, controlling every step of protein synthesis from initiation to elongation, termination, and quality control in positive as well as negative fashion. Through the study of eIF3, we are beginning to appreciate protein synthesis as a highly integrated process coordinating protein production with protein folding, subcellular targeting, and degradation. At the same time, eIF3 subunits appear to have specific functions that probably vary between different tissues and individual cells. Considering the broad functions of eIF3 in protein homeostasis, it comes as little surprise that eIF3 is increasingly implicated in major human diseases and first attempts at therapeutically targeting eIF3 have been undertaken. Much remains to be learned, however, about subunit- and tissue-specific functions of eIF3 in protein synthesis and disease and their regulation by environmental conditions and post-translational modifications.

scholarly journals P(VDF-TrFE) nanofibers: structure of the ferroelectric and paraelectric phases through IR and Raman spectroscopies

RSC Advances ◽  
2020 ◽  
Vol 10 (62) ◽  
pp. 37779-37796
Author(s):  
Alessia Arrigoni ◽  
Luigi Brambilla ◽  
Chiara Bertarelli ◽  
Gianluca Serra ◽  
Matteo Tommasini ◽  
...  
Keyword(s):  
Vinylidene Fluoride ◽  
Complex Morphology ◽  
Ir And Raman Spectroscopies ◽  
Poly Vinylidene Fluoride ◽  
Ir And Raman

This study elucidates the complex morphology and the related spectroscopic response of poly(vinylidene fluoride-co-trifluoroethylene) copolymer, with 80% molar VDF content, namely P(VDF-TrFE) (80/20).

scholarly journals Alp Inhibitors: Vanadyl(IV) Complexes Of Ferulic And Cinnamic Acid

2005 ◽  
Vol 60 (3) ◽  
pp. 305-311 ◽  
Author(s):  
Evelina G. Ferrer ◽  
María V. Salinas ◽  
María J. Correa ◽  
Fernanda Vrdoljak ◽  
Patricia A. M. Williams
Keyword(s):  
Alkaline Phosphatase ◽  
Cinnamic Acid ◽  
Alkaline Phosphatase Activity ◽  
Elemental Analysis ◽  
Diffuse Reflectance ◽  
Quinic Acid ◽  
Inhibitory Effect ◽  
Cinnamic Acids ◽  
Ir And Raman Spectroscopies ◽  
Ir And Raman

Two new vanadyl(IV) carboxylate complexes have been obtained: Na2[VO(Fer)2(CH3OH)2] and Na2[VO(Cin)2(CH3O)2] and characterized by elemental analysis and UV-vis, diffuse reflectance and IR and Raman spectroscopies (FerH2 = ferulic acid, CinH= cinnamic acid). The thermal behavior was also investigated. The inhibitory effect on alkaline phosphatase activity was tested for the compounds and ferulic and cinnamic acids as well as for the vanadyl(IV) complex of quinic acid for comparison. The ferulic complex together with the free ligands exhibited the lowest inhibitory effect, while the VO/quinic and VO/cinnamic complexes showed an intermediate inhibition potential

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