Development of an Insulin Nano-Delivery System through Buccal Administration

2022 ◽  
Vol 19 ◽  
Author(s):  
Diaa Al-Domi ◽  
Ayat Bozeya ◽  
Mohamed Al-Fandi
Keyword(s):  
Insulin Release ◽  
Delivery System ◽  
Polymeric Nanoparticles ◽  
Sustained Delivery ◽  
Atomic Force ◽  
Oil In Water ◽  
In Vitro Investigation ◽  
Coating Method ◽  
Evaporation Technique

Aim: To develop a new nano-delivery system for insulin buccal administration. Background: Biodegradable polymeric nanoparticles (PNPs) had viewed countless breakthroughs in drug delivery systems. The main objective of PNPs application in delivering and carrying different promising drugs is to make sure that the drugs being delivered to their action sites. As a result maximizing the desired effect and overcoming their limitations and drawbacks. Objectives: The main goals of this study were to produce an insulin consumable nano-delivery system for buccal administration and enhance the mucoadhesive effect in sustaining insulin release. Methods: Water in oil in water (W-O-W) microemulsion solvent evaporation technique was used for the preparation of nanoparticles consisting from positively charged poly (D, L-lactide-co-glycolide) coated with chitosan and loaded with insulin. Later, a consumable buccal film was prepared by the spin coating method and loaded with the previously prepared nanoparticles. Results: The newly prepared nanoparticle was assessed in terms of size, charge and surface morphology using a Scanning Electron Microscope (SEM), zeta potential, Atomic Force Microscope (AFM), and Fourier Transform Infra-red (FTIR) spectroscopy. An in-vitro investigation of the insulin release, from nanoparticles and buccal film, demonstrated controlled as well as sustained delivery over 6 hrs. The cumulative insulin release decreased to about (28.9%) with buccal film in comparing with the nanoparticle (50 %). Conclusion: The buccal film added another barrier for insulin release. Therefore, the release was sustained.

scholarly journals Fabrication of PLA-PEG Nanoparticles as Delivery Systems for Improved Stability and Controlled Release of Catechin

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Neha Atulkumar Singh ◽  
Abul Kalam Azad Mandal ◽  
Zaved Ahmed Khan
Keyword(s):  
Controlled Release ◽  
Delivery System ◽  
Oral Delivery ◽  
Polymeric Nanoparticles ◽  
Double Emulsion ◽  
Hydrodynamic Diameter ◽  
Sem Image ◽  
Oral Delivery System ◽  
Improved Stability

The purpose of this study was to develop an oral delivery system for the controlled release of catechin and evaluate the antioxidant potential and stability of catechin loaded PLA/PEG nanoparticles (CATNP). Nanoparticles were synthesized using a double emulsion solvent evaporation method. The fabricated nanoparticles were relatively small with a hydrodynamic diameter of 300 nm and an encapsulation efficiency of 95%. SEM image analysis showed uniform sized and spherically shaped nanoparticles. In vitro release profiles indicated a slow and sustained release of catechin from the nanoparticle. Stability of the nanoparticle in simulated gastric and intestinal fluids is maintained due to the PEG coating on the nanoparticles, which effectively protected catechin against gastrointestinal enzyme activity. Enhanced inhibition action of free radicals and metal chelation potential was noted when catechin was encapsulated in these polymeric nanoparticles. The reports obtained from this study would provide an opportunity for designing an oral delivery system aimed at inhibiting oxidative stress in the human body.

Development of pellets of nifedipine using HPMC K15 M and κ-carrageenan as mucoadhesive sustained delivery system and in vitro evaluation

2013 ◽  
Vol 22 (12) ◽  
pp. 911-921 ◽  
Author(s):  
Pradum Pundlikrao Ige ◽  
Pravin Rajput ◽  
Chandrakantsingh Pardeshi ◽  
Rajendra Kawade ◽  
Bramhanand Swami ◽  
...  
Keyword(s):  
Delivery System ◽  
Sustained Delivery ◽  
In Vitro Evaluation

pH Responsive Polymeric Nanoparticles for Oral Insulin Delivery

2017 ◽  
pp. 6-10
Author(s):  
Charu Tyagi
Keyword(s):  
Insulin Release ◽  
Polymeric Nanoparticles ◽  
Attenuated Total Reflectance ◽  
Ph Responsive ◽  
Eudragit L100 ◽  
Release Studies ◽  
Mean Size ◽  
The Mean ◽  
In Vitro Insulin Release

Gelatin-eudragit L100 nanoparticles of wet size range 170-563nm were prepared by two step dissolvation method and the effect of different concentrations of eudragit L100 and emulsifying agent - sodium lauryl sulphate (SLS) - on the particle size were studied. Synthesized nanoparticles were characterized by attenuated total reflectance-fourier transform infrared spectroscopy (ATRFTIR) and the mean size distribution. Insulin loading was done at a pH 7.4 and the in vitro insulin release studies of nanoparticles were carried out by simulating gastrointestinal tract condition which showed the minimal insulin release at pH 2.5 (20% in 90min) while appreciable release (40% in first 30min) at pH of 7.4. This pH responsive release pattern of the synthesized nanoparticles confers on the insulin protection from proteolytic degradation in acidic environment of stomach and upper intestinal part while enhancing bioavailability in the later part of intestine.

The high potency of polymeric nanoparticles in the drug delivery system for hypertension treatment; A systematic review

2021 ◽  
Vol 17 ◽  
Author(s):  
Fatemeh Mohammadipour ◽  
Aliasghar Kiani ◽  
Arash Amin
Keyword(s):  
Systematic Review ◽  
Drug Delivery ◽  
Drug Delivery System ◽  
Delivery System ◽  
Oral Absorption ◽  
Polymeric Nanoparticles ◽  
Hypertension Treatment ◽  
High Potency

Background: Polymeric nanomaterials with size ranging from 10 to 1000 nm are one of the most widely used types of nanoparticles with ideal properties in the drug delivery systems. Here, we decided to systematically review the antihypertensive effects of polymeric nanomaterials in vitro, in vivo, and clinical trials. Methods: The present review was conducted based on the 06- PRISMA guideline; whereas five English databases, including Scopus, PubMed, Web of Science, EMBASE, and Google Scholar without time limitation were used for searching the publications related to antihypertensive effects of natural and synthetic polymeric nanoparticles. Results: The results demonstrated that among 1701 papers, 25 papers including 11 in vitro (44%), 6 in vivo (24%), 7 in vitro / in vivo (28%), and 1 in vitro / ex vivo (4%) up to 2020, met the inclusion criteria for discussion in this systematic review. The most used nanoparticles poly-(lactic-co-glycolic) acid nanoparticle (PLGANPs) (7, 29.2%), chitosan based nanoparticles (6, 25%), followed by polylactide acid nanoparticles (5, 20.8%). Conclusion: We concluded that the high potency of polymeric nanoparticles in the drug delivery system for hypertension treatment. Although the accurate mechanisms are not fully understood; however, some mechanisms such as sustained release forms with increased bioavailability, increasing oral bioavailability and improve the oral and non-oral absorption, counteracting excessive superoxide and decreasing blood pressure, etc can be related these nanoparticles.

Antitumor Cytokine DR5-B-Conjugated Polymeric Poly(N-vinylpyrrolidone) Nanoparticles with Enhanced Cytotoxicity in Human Colon Carcinoma 3D Cell Spheroids

2021 ◽  
Vol 7 (1) ◽  
pp. 8
Author(s):  
Anne Yagolovich ◽  
Andrey Kuskov ◽  
Pavel Kulikov ◽  
Leily Kurbanova ◽  
Anastasia Gileva ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Colon Carcinoma ◽  
Delivery System ◽  
Polymeric Micelles ◽  
Polymeric Nanoparticles ◽  
Human Colon ◽  
Hydrophobic Core ◽  
Human Colon Carcinoma ◽  
Self Assembled

Self-assembled nanoparticles based on amphiphilic poly(N-vinylpyrrolidone) (Amph-PVP) were proposed earlier as a new drug delivery system. In the current work, we study the antitumor activity of Amph-PVP-based self-assembled polymeric micelles covalently conjugated with the antitumor receptor-specific TRAIL variant DR5-B (P-DR5-B). The Amph-PVP polymer was synthesized by the earlier developed one-step technique (Kulikov et al., Polym. Sci. Ser. D, 2017). To stabilize Amph-PVP associates, the hydrophobic core was loaded with the model substance prothionamide. For the covalent conjugation with DR5-B, the hydrophilic ends of polymeric chains were modified with maleimide, and a DR5-B N-terminal amino acid residue valine was mutated to cysteine (DR5-B/V114C). DR5-B/V114C was conjugated to the surface of polymeric micelles by the selective covalent interaction of N-terminal cysteine residue with maleimide on Amph-PVP. The cytotoxicity of DR5-B-conjugated Amph-PVP polymeric nanoparticles was investigated in 3D multicellular tumor spheroids (MCTS) of human colon carcinoma HCT116 and HT29 cells, generated by the RGD-induced self-assembly technique (Akasov et al., Int. J. Pharm., 2016). In DR5-B-sensitive HCT116 MCTS, the P-DR5-B activity slightly increased compared to that of DR5-B. However, in DR5-B-resistant HT29 MCTS, P-DR5-B significantly surpassed DR5-B in the antitumor activity. Thus, the conjugation of DR5-B with the Amph-PVP nanoparticles enhanced its tumor-cell killing capacity. In the current study, we obtain a new nano-scaled delivery system based on Amph-PVP self-aggregates coated with covalently conjugated antitumor DR5-specific cytokine DR5-B. P-DR5-B overcomes DR5-B-resistance of the human colon carcinoma MCTS in vitro. This makes Amph-PVP polymeric nanoparticles a prospective and versatile nano-scaled delivery system for the targeted proteins.

scholarly journals In vitro and in vivo investigation of chitosan–polylysine polymeric nanoparticles for ovalbumin and CpG co-delivery

RSC Advances ◽  
2017 ◽  
Vol 7 (63) ◽  
pp. 39962-39969 ◽  
Author(s):  
Yunfei Han ◽  
Qian Duan ◽  
Yanhui Li ◽  
Jian Tian
Keyword(s):  
Ethylene Glycol ◽  
Delivery System ◽  
Polymeric Nanoparticles ◽  
Poly Ethylene Glycol ◽  
Glycol Chitosan ◽  
Vaccine Delivery System ◽  
Electrostatic Binding ◽  
Poly Ethylene

A simple and powerful vaccine delivery system was developed by electrostatic binding of chitosan-based polycation methoxy poly(ethylene glycol)–chitosan–poly(l-lysine) (mPEG–CS–PLL) with ovalbumin (OVA) and cytosine–phosphate–guanine (CpG).

scholarly journals Fabrication and Development of Pectin Microsphere of Metformin Hydrochloride

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Pritam Banerjee ◽  
Jyotirmoy Deb ◽  
Amitava Roy ◽  
Amitava Ghosh ◽  
Prithviraj Chakraborty
Keyword(s):  
Delivery System ◽  
Metformin Hydrochloride ◽  
Physical Parameters ◽  
Dissolution Profile ◽  
High Release ◽  
Evaporation Technique ◽  
Novel Drug ◽  
Evaluation Parameters

Purpose. The objective of the proposed work is to evaluate the efficacy of Pectins to qualify them as polymers for designing an oral microsphere for the delivery of selected oral antidiabetic drug-like metformin hydrochloride. Methods. Different Microspheres formulations were prepared by the water in oil (w\o) emulsion solvent evaporation technique and subsequently evaluated for its different physical parameters as well as its in vitro and in vivo drug release study. Results. The formulations F2 (98.42) and F3 (98.03) showed a constant and high release in the dissolution profile, so among these two formulations, F2 was taken for development study, due to the better result shown over in other evaluation parameters. From the HPLC determinations after in vivo study, it had been found that the test samples and the standard sample had not shown any significant fluctuation in relation to their retention time. Conclusion. From in vitro and in  vivo results, it may be concluded that drug-loaded pectin microspheres in 1 : 1 ratio are a suitable delivery system for metformin hydrochloride and may be used for effective management of NIDDM. From this experiment, it could be concluded that as a natural polymer, pectin has potentiality in novel drug delivery system.

scholarly journals OPTIMIZATION AND CHARACTERIZATION OF RIVASTIGMINE-LOADED NANOSTRUCTURED LIPID CARRIERS

2021 ◽  
pp. 46-51
Author(s):  
SWATHI GANNA ◽  
SAI MANOGNA KOTAKADI ◽  
RESHMA ANJUM MOHAMMED ◽  
MANNUR ISMAIL SHAIK ◽  
JOHN SUSHMA NANNEPAGA
Keyword(s):  
Particle Size ◽  
Chemical Interaction ◽  
Surfactant Solution ◽  
Entrapment Efficiency ◽  
Nanostructured Lipid Carriers ◽  
Sustained Delivery ◽  
Scanning Calorimetry ◽  
Atomic Force ◽  
Dsc Analyses

Objective: The objective of the present study was to develop Nanostructured lipid carriers (NLCs) for improvement in the oral bioavailability of RT. Methods: RT-loaded NLCs were prepared by high shear homogenization technique using fish oil and flaxseed oil respectively. The prepared RT-NLCs were characterized using a phase-contrast microscope, scanning electron microscope (SEM), atomic force microscope (AFM), Fourier transform-infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC). Further, particle size, entrapment efficiency and sustained release of the drug were also studied. Results: SEM results revealed that the RT-NLCs were spherical in shape with a smooth surface. AFM results confirmed the formation of spherical particle dispersions by the NLCs in nanoscale. FTIR spectroscopy and DSC analyses revealed that there is no chemical interaction between the ingredients of RT-NLCs. The particle size of the RT-NLCs was found to be exponentially decreased with the increase in a surfactant solution. Conclusion: The results confirmed pronounced improvement in entrapment efficiency of optimized formulation of RT-NLCs. In vitro, drug release studies showed that RT-NLCs were capable of releasing the drug in a sustained manner. The experimental results showed that the NLCs are potential carriers for providing sustained delivery of rivastigmine.

Synthesis and Characterization of Magnetic Polymeric Nanospheres for Biomedical Applications

2002 ◽  
Vol 750 ◽  
Author(s):  
D. K. Kim ◽  
M. Mikhaylova ◽  
M. Toprak ◽  
A. Guyou ◽  
Y. K. Jeong ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Polymeric Nanoparticles ◽  
Oil Emulsion ◽  
Diffusion Technique ◽  
Oil In Water ◽  
Anti Cancer ◽  
Evaporation Technique ◽  
Water In Oil Emulsion ◽  
Polymeric Nanospheres

ABSTRACTEntrapment of proteins in biodegradable nanospheres has been widely investigated as a technique to produce sustained release formulations for protein or anti-cancer drugs administration. Amphiphilic PLLA-mPEG diblock copolymer was prepared by ring opening polymerization (ROP) to form polymeric nanoparticles with a core-shell structure. The main encapsulation technique done is a water-in-oil-in-water (w/o/w) solvent evaporation technique. Here, protein was encapsulated using a newly developed water-in-oil emulsion-solvent diffusion technique. This technique leads to the formation of an emulsion combined with the immediate precipitation of the PLLA-mPEG. This phenomenon is caused by the diffusion of the polymer solvent to an external organic phase.

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