Domestic Processing Effects on Antioxidant Capacity, Total Phenols and Phytate Content of Sorghum

2020 ◽  
Vol 16 (4) ◽  
pp. 501-507
Author(s):  
Érica A. Moraes ◽  
Fernanda C.E. de Oliveira ◽  
Valéria A. Vieira Queiroz ◽  
Robert E. Schaffert ◽  
Paulo R. Cecon ◽  
...  

Background: Whole grains may reduce chronic disease risks and these benefits are related to food matrix components, especially phenolic acids. However, food processing may change the total phenol content and antioxidant capacity. Objective: This study aims to evaluate the effect of processing on the antioxidant capacity and total phenols, tannins and phytate contents in the sorghum grain of seven genotypes. Methods: Antioxidant capacity (AC), total phenolic content (TPC), tannins and phytate content were evaluated in unheated sorghum grains (USG), heated sorghum grains (HSG) and popped sorghum grains (PSG) of seven genotypes. Results: AC ranged from 66.5% to 92.9% in USG, decreased after PSG in all genotypes, and maintained after HSG, except for BRS 506. TPC and tannins were higher in BRS 305. PSG decreased tannin content in all genotypes. TPC of HSG was higher than the USG in BRS 310, BRS 305, BR 501 and BR 700 genotypes. PSG affected TPC content mainly in BRS 305 and BR 700. AC and tannins were especially affected by the popping conditions. There was no difference in the phytate content among all genotypes, which were maintained after HSG in BRS 506, BRS 305, BR 700 and CMSXS 136. Conclusion: AC, TPC, tannins and phytate content were affected by the processing conditions, however, those changes may be related to the compounds type and sorghum genotype.

Plants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 599
Author(s):  
Živilė Tarasevičienė ◽  
Aloyzas Velička ◽  
Aurelija Paulauskienė

Phenolic compounds have a number of benefits to human health and can be used as preventive compounds for the development of some chronic diseases. Mentha plants are not only a good source of essential oils, but also contain significant levels of wide range of phenolic compounds. The aim of this research was to investigate the possibility to increase phenols content in Mentha plants under the foliar application with L-phenylalanine, L-tryptophan, L-tyrosine at two concentrations (100 mg L−1 and 200 mg L−1) and to create preconditions for using this plant for even more diverse purposes. Quantitative and qualitative analyses of phenols in mints were performed by HPLC method. Foliar application of amino acids increased the total phenol content from 1.22 to 3.51 times depending on the treatment and mint variety. The most pronounced foliar application to total phenols content was tryptophane especially in Mentha piperita “Swiss”. Mentha piperita “Swiss” was affected most by foliar application and the amount of total phenolic acids depending on the treatment ranged from 159.25 to 664.03 mg 100 g−1 (DW), respectively, non-sprayed and sprayed with tryptophane 100 mg L−1. Our results suggest that the biophenol content varies according to such factors as foliar application and variety, and every single mint variety has individual response to different applications of amino acids.


2018 ◽  
Vol 47 (2) ◽  
pp. 390-394 ◽  
Author(s):  
Sina N. COSMULESCU ◽  
Ion TRANDAFIR ◽  
Felicia CORNESCU

A number of selected cornelian cherry wild genotypes were investigated regarding antioxidant capacity, total phenols, total flavonoids and colour component. The total phenolic, total flavonoids and antioxidant activity of methanol extract of cornelian cherry fruits were determined using Folin-Ciocalteu, colorimetric and DPPH methods, respectively. Total phenolic content ranged from 163.69 (S1) to 359.28 (H2) mg GAE 100 g-1 FW. Genotypes H2 and H3 had the highest total phenolic content (359.28 and 343.50 mg GAE 100 g-1 FW, respectively), total flavonoids (54.26 and 64.48 mg QE 100 g-1 FW, respectively) and antioxidant capacity (2.39 and 2.71 mmolTrolox100g-1 FW, respectively). Significant differences of yellow/blue color component (b*), red/ green (a*) color component, values of lightness (L*) and chroma (C) were obtained for extracts from fruits. The present study shows the potential of certain cornelian cherry genotypes as valuable source of natural antioxidants and gene stock for breeding programs.


2017 ◽  
Vol 45 (2) ◽  
pp. 553-560 ◽  
Author(s):  
Vasile LASLO ◽  
Alin C. TEUSDEA ◽  
Sonia A. SOCACI ◽  
Daniel MIERLITA ◽  
Simona I. VICAS

Peach and nectarine (Prunus persica ) production has an important place in the world, being the most important fruit after apple crops in the European Union. Because the fruits are perishable, it is desirable to valorize them as juice. Seven peaches and three nectarines cultivars grown in the N-W part of Romania were investigated for quality parameters, volatile profile, total phenols content and antioxidant capacity. The volatile composition of peach and nectarine cultivars was determined via the ITEX/GC-MS technique, the main volatile compounds belonging to alcohols and aldehydes. Another objective was to obtain the pasteurised juices from these fruits and to investigate the best time of pasteurisation in order to identify the most valuable cultivar from the perspective of total phenols content and its antioxidant capacity. For a better interpretation of results and a proper discrimination between cultivars, according to the total phenols content and antioxidant capacity, the multivariate analysis, Principal Component Analysis (PCA) and Hierarchical Cluster Analysis (HCA) were applied. The peach cultivars showed the highest content in total phenols compared with nectarine. From peach cultivars, the highest concentration was recorded in ‘Southland’ (47.49 ± 0.14 mg GAE 100 g-1 FW) and from nectarine cultivars in ‘Romamer’ (16.28 ± 0.83 mg GAE 100 g-1 FW). The highest antioxidant capacities were recorded in ‘Southland’ peach in the case of both methods (DPPH and FRAP). The results showed that ‘Southland’ peach and ‘Romamer’ nectarine pasteurised juices are the best from the point of view of total phenolic compounds content with high antioxidant capacity.


Foods ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 216
Author(s):  
Jakub Frankowski ◽  
Anna Przybylska-Balcerek ◽  
Kinga Stuper-Szablewska

More than 35% of the world sorghum seed production is a human food source. The main ingredient of fully ripe sorghum grains is starch. Sorghum does not contain gluten, and it is also a rich source of antioxidant compounds other than vitamins or macro- and microelements, including phenolic acids, flavonoids, and sterols. The aim of this study was to determine the antioxidant activity and the content of selected bioactive compounds, i.e., total phenolic acids, total flavonoids, and total phytosterols, as well as determination of the qualitative and quantitative profile of phenolic acids, flavonoids, and phytosterols in various food products, the basic ingredient of which was sorghum grain. It was found that antioxidant activity is related to the total phenolic compounds content. The ABTS•+ ranged from 319 to 885 µmol TROLOX/kg. However, white sorghum grain flour contained almost two times more polyphenols than red sorghum grain flour. The FPA ranged from 224 in raw pasta to 689 mgGAE/100 g in white sorghum grain. During this study, the quantitative profile of selected polyphenols in grain flour, wafers, pasta, and cookies containing sorghum grain was also investigated, as well as the content of 11 selected phenolic acids. Total content of the latter ranged from 445 to 2850 mg/kg. Phytosterols such as beta-sitosterol, campesterol, and stigmasterol were found in all the analyzed products. Based on this research, it was investigated that the products containing sorghum grains can be classified as functional food.


2016 ◽  
Vol 44 (2) ◽  
pp. 28-33 ◽  
Author(s):  
Geovanna Tafurt García ◽  
Luisa Jiménez Vidal ◽  
Ana Calvo Salamanca

<p>In this work, the possible correlation between the antioxidant activities and the Total Phenolic Content (TPC) and chemical composition of Lamiaceae (<em>H. conferta, H. dilatata, H. mutabilis, H. suaveolens</em>), Burseraceae (<em>P. heptaphyllum, T. rhoifoila, T. panamensis</em>),<em> </em>and Lauraceae (<em>Ocotea </em>sp.) were evaluated. The Trolox Equivalent Antioxidant Capacity or the Total Antioxidant Activity (TAA) was determined by using a colorimetric assay with the ABTS radical cation, Effective Concentration (EC<sub>50</sub>) was evaluated with the DPPH radical, and the TPC was established by the Folin-Ciocalteu method, for ethanolic extracts obtained by cold maceration and evaporation to dryness. Both the TAA and the EC<sub>50</sub> were highly correlated with the TPC. The barks of <em>T. rhoifolia</em> and<em> T. panamensis</em> demonstrated the highest antioxidant capacities. The Burseraceae spp. exhibited the highest TPC, and the Lamiaceae (<em>Hyptis</em> spp.) demonstrated the lowest TPC.</p>


Author(s):  
Vasile LASLO ◽  
Sonia SOCACI ◽  
Alin TEUSDEA ◽  
Adrian TIMAR ◽  
Maria TOFANĂ ◽  
...  

The objective of this study was to characterize two varieties of apple (Florina and Liberty) grown in the North-West of Romania, with a focus on their volatile compounds, bioactive compounds and their antioxidant capacity. The content of bioactive compounds and antioxidant capacity was also tracked during pasteurization at different times (15, 20 and 25 minutes). Among bioactive compounds, the total phenol content was determined, as well as the total flavonoid and vitamin C content. The antioxidant capacity of fruits and pasteurized juice was evaluated by three different methods (DPPH, FRAP and ABTS). Of the 2 apple cultivars, Florina showed a remarkably higher content of total phenolic compounds (657.97 mg GAE/kg fw), flavonoids (122.07 mg QE/kg fw) and vitamin C (94.62 mg/kg), compared to the Liberty cultivar. Following pasteurization, the vitamin C contents decreased significantly relative to pasteurization time. However, in the case of total phenols content, only insignificant decreases were registered, compared to unpasteurized juice. The content of total flavonoids increased significantly after 15 minutes of pasteurization in apple cultivars juices. The apple varieties investigated are rich in bioactive compounds, and pasteurization treatment does not lead to drastic decreases in these compounds and in the antioxidant capacity of apple juices.


2019 ◽  
Vol 2019 ◽  
pp. 1-14 ◽  
Author(s):  
Thamizhiniyan Venkatesan ◽  
Young-Woong Choi ◽  
Young-Kyoon Kim

It is well established that various extraction factors, including the method, temperature, time, and solvent system, significantly influence the antioxidant quality of plant-derived products. Previously, we observed that extraction of Pinus densiflora bark (PDB) by the most common traditional Soxhlet method using water at two different temperature conditions 60°C and 100°C for 6-15 h noticeably altered their antioxidant quality. In this study, we examined the impact of different extraction solvents such as ethanol, methanol, isopropanol, acetonitrile, and acetone at a different percentage with water (vol/vol) on antioxidant efficiency as well as the total phenolic content (TPC) of PDB extracts. Among the fourteen different PDB extracts, the extracts obtained from 20% ethanol (E20), 40% ethanol (E40), and 20% acetonitrile (ACN20) showed more significant antioxidant potential, as well as high total phenol content (TPC). Extracts from other aqueous mixtures of organic solvents such as isopropanol, acetone, and methanol, as well as water, showed lesser antioxidant capacity and also had less TPC compared to these three most active extracts, E20, E40, and ACN20. Moreover, using ethanol at 100% for extraction significantly decreased the TPC and antioxidant capacity of PDB extracts. Data are implicating that an increased phenolic content in PDB extracts proportionally increases their antioxidant efficiency.


Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1432
Author(s):  
Alejandro Rubio-Melgarejo ◽  
Rosendo Balois-Morales ◽  
Verónica Alhelí Ochoa-Jiménez ◽  
Paloma Patricia Casas-Junco ◽  
José Orlando Jiménez-Zurita ◽  
...  

Soursop fruit (Annona muricata L.) production is diminished by the attack of pathogens such as Nectria haematococca. However, the fruit–pathogen interaction at the biochemical and molecular levels is still unknown. The objective of this study was to analyze the response of the soursop fruit to the presence of N. haematococca during postharvest storage. Soursop fruits were inoculated with the pathogen and total phenolic compounds, antioxidant capacity by Ferric reducing/antioxidant power (FRAP), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS•+), and 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH•), as well as enzymatic activity and transcript levels of polyphenol oxidase (PPO) and superoxide dismutase (SOD), were evaluated at 1, 3, and 5 days of storage. The noninoculated fruits were the controls of the experiment. The highest total phenol content was recorded on day one in the inoculated fruits. FRAP, ABTS, and DPPH activity presented the highest values on day three in the control fruits. Inoculated fruits recorded the highest PPO activity on day five and a five-fold induction in the PPO transcript on day three. SOD activity showed a decrease during the days of storage and 10-fold induction of SOD transcript on day three in the inoculated fruits. Principal component analysis showed that total phenols were the variable that contributed the most to the observed variations. Furthermore, a positive correlation between total phenols and SOD activity, PPO expression, and SOD expression, as well as between DPPH and FRAP, was recorded. The results showed a differential response in antioxidant capacity, enzymatic activity, and gene expression during the interaction of soursop fruits–N. haematococca at postharvest storage.


2019 ◽  
pp. 49-59
Author(s):  
Nu Linh Giang Ton ◽  
Thi Hoai Nguyen ◽  
Quoc Hung Vo

Avocado peel has been considered as a potential source of natural antioxidants in which phenolics are among the most important compounds. Therefore, this study aims to optimize the extraction process of phenolics using response surface methodology and evaluate the corresponding antioxidant activity. From the quadratic model, the optimal condition was determined including the ethanol concentration 54.55% (v/v), the solvent/solute ratio 71.82/1 (mL/g), temperature 53.03 oC and extraction time 99.09 min. The total phenolic content and the total antioxidant capacity at this condition with minor modifications were 26,74 ± 0,04 (mg GAE/g DW) and 188.06 ± 1.41 (mg AAE/g DW), respectively. The significant correlation between total phenolic content and total antioxidant capacity was also confirmed. Key words: response surface methodology, central composite rotatable design, total phenolic content, total antioxidant capacity, avocado peel


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