Characterization of Chemical Compounds and Antioxidant Activity of Centaurea solstitialis sp. schouwii (DC.) Q. et S. (Asteraceae)

Background: The antioxidant activity and the total phenolic and flavonoid contents of the derived extracts (chloroform, ethyl acetate and n-butanol) of the 70% hydroalcoholic extract of the aerial parts of Centaurea solstitialis growing in Algeria was assessed. The active extracts were selected for phytochemical investigations. Methods: The antioxidant capabilities of the extracts were assessed using 1, 1-diphenyl-2- picrylhydrazyl radical (DPPH•) scavenging and Cupric Ion Reducing Antioxidant Capacity (CUPRAC) assays. Butylhydroxyanisole (BHA), butylhydroxytoluene (BHT) and α –tocopherol were used as positive controls. The total phenolic content and total flavonoid content of the extracts were determined as gallic acid equivalents and quercetin equivalents, respectively. Chromatographic methods were used to isolate the secondary metabolites and spectrometric and spectroscopic methods were used to determine their chemical structures. Results: The ethyl acetate extract exhibited the highest antioxidant activities followed by the n-butanol extract. The highest phenolic and flavonoid contents were found in the n-butanol extract. Phytochemical study of the ethyl acetate and n-butanol extracts led to the isolation of an undescribed guaianolide named 3-(4-hydroxybenzoyl)-cynaratriol and a known sesquiterpene lactone along with three known flavonoid glycosides. Their structures were established by spectral analyzes mainly high resolution electrospray ionisation mass spectrometry (HR-ESIMS) and 1D and 2D nuclear magnetic resonance experiments. Conclusion: The extracts of aerial parts of C. solstitialis showed significant antioxidant activities. An undescribed sesquiterpene lactone and four known secondary metabolites were isolated from the most active extracts.

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NEW FLAVONOIDS FROM THE AERIAL PARTS OF POLYGONUM EQUISETIFORME SM (POLYGONACEAE)

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i2.15593 ◽

2017 ◽

Vol 9 (2) ◽

pp. 166 ◽

Cited By ~ 2

Author(s):

Sayed A. El-toumy ◽

Joslin Y. Salib ◽

Nabila H. Shafik ◽

Asmaa S. Abd Elkarim ◽

Gihan A. Mick

Keyword(s):

Antioxidant Activity ◽

Methyl Ester ◽

Secondary Metabolites ◽

Gallic Acid ◽

Total Phenolic ◽

Flavonoid Content ◽

Isolation And Identification ◽

Aerial Parts ◽

Dpph Method ◽

Phenolic And Flavonoid

Objective: The current study was to deal the isolation and identification of secondary metabolites from Polygonum equisetiforme and evaluation of antioxidant activity of its extract.Methods: The methanol-water extract (7:3) of the air-dried aerial parts of Polygonum equisetiforme was fractionated and separated to obtain the isolated compounds by different chromatographic techniques. Structures of these compounds were elucidated by UV and 1D⁄2D H⁄ C NMR spectroscopy and compared with the literature data. The crude extract was evaluated for in vitro antioxidant activity using the 2,2 diphenyl dipicryl hydrazine (DPPH) method.Results: Ten secondary metabolites were isolated from Polygonum equisetiforme in this study. Of which three new flavonoids named as 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside (1), 3,5,7,2’,5’ pentahydroxyflavone 3-O-b-D-glucopyranoside 8 C-sulphated (2) and quercetin 3-O-β-D-glucucorinde 6''-methyl ester 8-sulphated (3) as well as quercetin 3-O-β-D-glucucorinde methyl ester (4), quercetin 3-O-β-D-glucopyranoside (5), quercetin 7-O-β-D-glucopyranoside (6), quercetin(7), myricetin (8), P-methoxy gallic acid methyl ester (9) and gallic acid (10). The antioxidant potential of P. equisetiforme extract was evaluated by investigating it's total phenolic and flavonoid content and DPPH radical scavenging activity whereby the extract showed significant antioxidant activity (IC50 = 37.45 μg/ml). The total phenolic and flavonoid content was found to be 130.79±5.502 and 45.8±1.63 μg/ml, respectively.Conclusion: Polygonum equisetiforme is a promising medicinal plant, and our study tends to support the therapeutic value of this plant as an antioxidant drug.

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Effect of Geographical and Seasonal Variations on Phenolic Contents and Antioxidant Activity of Aerial Parts of Urtica diocia L.

Current Traditional Medicine ◽

10.2174/2215083804666181012123333 ◽

2019 ◽

Vol 5 (2) ◽

pp. 159-167 ◽

Cited By ~ 2

Author(s):

Vinod Kumar Gautam ◽

Mannu Datta ◽

Ashish Baldi

Keyword(s):

Antioxidant Activity ◽

Secondary Metabolites ◽

Seasonal Variations ◽

Summer Season ◽

Antioxidant Potential ◽

Total Phenolic ◽

Spring Season ◽

Phenolic Contents ◽

Aerial Parts ◽

Phenolic And Flavonoid

Background: Environmental conditions affect the biosynthesis of secondary metabolites as a result of biotic and abiotic factors. In the present study, the effect of different geographical locations and season was studied on total phenolic and flavonoid contents extracted from Urtica dioica Linn. Methodology: The aerial parts of U. dioica collected from Palampur, Shimla and Dharamshala in different seasons were subjected to hydro-alcoholic extraction. Quantitative estimation of total phenolic and flavonoid contents in various extracts was carried out spectrophotometrically. Results: The highest amount of total phenolic (3.987± 0.130) and flavonoid contents (2.847± 0.341) was found in Palampur sample collected in summer season whereas sample collected from Dharamshala in spring season showed the least phenolic contents. In vitro antioxidant activity of all the samples was evaluated by DPPH, NO scavenging and FRPA method. The antioxidant potential was found maximum in the sample collected from Palampur in the summer season, however, the sample collected from Dharamshala in spring season showed the least antioxidant potential. Conclusion: The present study confirms that altitude and seasonal variations significantly affect the levels of secondary metabolites in plant parts.

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Volatile Fraction Composition and Total Phenolic and Flavonoid Contents of Elionurus hensii—Antioxidant Activities of Essential Oils and Solvent Extracts

Natural Product Communications ◽

10.1177/1934578x1300800528 ◽

2013 ◽

Vol 8 (5) ◽

pp. 1934578X1300800 ◽

Cited By ~ 4

Author(s):

Yin Yang ◽

Marie-Cécile De Cian ◽

Samuel Nsikabaka ◽

Pierre Tomi ◽

Thomas Silou ◽

...

Keyword(s):

Gas Chromatography ◽

Antioxidant Activity ◽

Essential Oil ◽

Ethyl Acetate ◽

Gas Chromatography Mass Spectrometry ◽

Total Phenolic ◽

Volatile Fraction ◽

Aerial Parts ◽

Reducing Ability ◽

Phenolic And Flavonoid

The objective of this study was to characterize Elionurus hensii in terms of the chemical composition of its essential oil and volatile fractions, the total phenolic and flavonoid contents of solvent extracts and antioxidative activity. Analysis of E. hensii essential oil samples from Congo was carried out using Gas Chromatography (GC) and Gas Chromatography-Mass Spectrometry (MS). The essential oil of the aerial parts consisted predominantly of oxygenated monoterpene compounds, of which the main components were menthane alcohol stereoisomers, whereas the essential oil of the root was characterized by a high content of oxygenated sesquiterpenes, the main component of which was aristolone. The amounts of total phenolics and flavonoids in the solvent extracts (dichloromethane, ethyl acetate and methanol) were determined spectrometrically. The essential oil did not exhibit significant antioxidant activity in terms of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid (ABTS•+) scavenging ability, and exhibited only slight Fe3+ reducing ability. In contrast, the antioxidant activity of the solvent extracts of the aerial parts increased in the order ethyl acetate > methanol > dichloromethane, and that of the solvent extracts of the root increased in the order methanol > ethyl acetate > dichloromethane. Finally, a relationship was observed between the antioxidant activity and the total phenolic and flavonoid levels of the extract.

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Antioxidant and Anticancer Activities of Wampee (Clausena lansium(Lour.) Skeels) Peel

Journal of Biomedicine and Biotechnology ◽

10.1155/2009/612805 ◽

2009 ◽

Vol 2009 ◽

pp. 1-6 ◽

Cited By ~ 29

Author(s):

K. Nagendra Prasad ◽

Jing Hao ◽

Chun Yi ◽

Dandan Zhang ◽

Shengxiang Qiu ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Reducing Power ◽

Total Phenolic ◽

Liver Carcinoma ◽

Scavenging Activity ◽

Anticancer Activities

Antioxidant activities of wampee peel extracts using five different solvents (ethanol, hexane, ethyl acetate, butanol and water) were determined by using in-vitro antioxidant models including total antioxidant capability, 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity, reducing power, and superoxide scavenging activity. Ethyl acetate fraction (EAF) exhibited the highest antioxidant activity compared to other fractions, even higher than synthetic antioxidant butylated hydroxyl toluene (BHT). In addition, the EAF exhibited strong anticancer activities against human gastric carcinoma (SGC-7901), human hepatocellular liver carcinoma (HepG-2) and human lung adenocarcinoma (A-549) cancer cell lines, higher than cisplatin, a conventional anticancer drug. The total phenolic content of wampee fraction was positively correlated with the antioxidant activity. This is the first report on the antioxidant and anticancer activities of the wampee peel extract. Thus, wampee peel can be used potentially as a readily accessible source of natural antioxidants and a possible pharmaceutical supplement.

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Chemical Composition, Production of Secondary Metabolites and Antioxidant Activity in Coffee Cultivars Susceptible and Partially Resistant to Bacterial Halo Blight

Plants ◽

10.3390/plants10091915 ◽

2021 ◽

Vol 10 (9) ◽

pp. 1915

Author(s):

Joyce Alves Goulart da Silva ◽

Mário Lúcio Vilela de Resende ◽

Ingridy Simone Ribeiro ◽

Adriene Ribeiro Lima ◽

Luiz Roberto Marques Albuquerque ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Secondary Metabolites ◽

Pseudomonas Syringae ◽

Total Phenolic ◽

Leaf Extracts ◽

Defense Strategies ◽

Halo Blight ◽

Phenolic Profiles ◽

Phenolic And Flavonoid

Coffee production is one of the main agricultural activities in Brazil, and several coffee cultivars with disease resistance have already been developed. The secondary metabolites produced by plants are closely associated with defense strategies, and the resistance of coffee cultivars to bacterial halo blight (BHB) can be related to these compounds. Therefore, this study aims to compare a partially resistant coffee cultivar (Iapar-59) and a susceptible cultivar (Mundo Novo 376/4) to BHB (Pseudomonas syringae pv. garcae) in relation to the chemical composition and antioxidant activity of the leaf extracts. In addition, this study determined the total phenolic and flavonoid contents and phenolic profiles of the Iapar-59 leaf extracts of plants inoculated with P. syringae pv. garcae. The Iapar-59 extract showed a higher content of phenolic compounds and flavonoids than the Mundo Novo 376/4 extract. Both cultivars contained gallic, chlorogenic and caffeic acids; however, the highest contents were quantified in the Iapar-59 cultivar. The leaf extracts from the Iapar-59 cultivar exhibited higher antioxidant activity. Higher concentrations of gallic, caffeic and chlorogenic acids and the presence of vanillin were detected in the extract of cultivar Iapar-59 inoculated with P. syringae pv. garcae.

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Two New Prenylated Isoflavonoids from Erinacea anthyllis with Antioxidant and Antibacterial Activities

Natural Product Communications ◽

10.1177/1934578x1701200716 ◽

2017 ◽

Vol 12 (7) ◽

pp. 1934578X1701200 ◽

Cited By ~ 1

Author(s):

Soumia Mouffouk ◽

Laurence Marcourt ◽

Mohammed Benkhaled ◽

Kaouthar Boudiaf ◽

Jean-Luc Wolfender ◽

...

Keyword(s):

Antioxidant Activities ◽

Optical Rotation ◽

2D Nmr ◽

Antibacterial Activities ◽

Total Phenolic ◽

Aerial Parts ◽

Whole Plant ◽

The Family ◽

Chemotaxonomic Markers ◽

Phenolic And Flavonoid

Two new prenylated isoflavonoids, namely Erinasone A and Erinasone B along with 19 known secondary metabolites, including twelve isoflavonoids, three polyphenols, one flavonol, two flavanones and three steroids, were isolated from the whole plant (roots and aerial parts) of Erinacea anthyllis. Structures of all isolated compounds were elucidated by spectroscopic analysis, including 1D and 2D NMR (1H, 13C, COSY, HSQC, TOCSY, HMBC and NOESY), mass spectrometry (ESI-MS), UV-Vis, measurement of optical rotation [α]D and by comparison with the literature data. The total phenolic and flavonoid contents were determined in this study. Furthermore, the antioxidant and antibacterial activities of the EtOAc and n-BuOH extracts of E. anthyllis were evaluated. These extracts exhibited moderate antibacterial and antioxidant activities. Their IC50 values were approximately 0.04 mg/mL ( n-BuOH) and 0.037 mg/mL (EtOAc). Consequently, Erinacea anthyllis is a rich source of polyphenolic compounds particularly isoflavonoids used as chemotaxonomic markers for the subfamily Papilionoideae of the family Fabaceae.

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Phenolic Compounds from An Algerian Endemic Species of Hypochaeris laevigata var. hipponensis and Investigation of Antioxidant Activities

Plants ◽

10.3390/plants9040514 ◽

2020 ◽

Vol 9 (4) ◽

pp. 514 ◽

Cited By ~ 1

Author(s):

Nabila Souilah ◽

Zain Ullah ◽

Hamdi Bendif ◽

Kamel Medjroubi ◽

Tahar Hazmoune ◽

...

Keyword(s):

Phenolic Compounds ◽

Antioxidant Activities ◽

Quinic Acid ◽

Total Phenolic ◽

Antioxidant Action ◽

Endemic Plant ◽

Aerial Parts ◽

Phenolic Constituents ◽

First Time ◽

Phenolic And Flavonoid

Hypochaeris laevigata var. hipponensis (Asteraceae) is an endemic plant from Algeria. In the current study, we analyzed for the first time its chemical composition, especially phenolic constituents of dichloromethane (DCM), ethyl acetate (EA), and n-butanol (BuOH) fractionsof the aerial parts of Hypochaeris laevigata var. hipponensis by liquid chromatography-mass spectrometry (LC-MS/MS). The number of phenolic compounds detected in DCM, EA, and BuOH fractions were found to be 9, 20, and 15, respectively. More specifically, 12 phenolic acids were detected. Among them, quinic acid, chlorogenic acid, and caffeic acid were the most abundant ones. Meanwhile, only seven flavonoids were detected. Among them, rutin, apigetrin, and isoquercitrin were the major ones. We also determined the total phenolic and flavonoid contents, and fraction EA showed the highest values, followed by BuOH, and DCM fractions. Furthermore, the antioxidant action was dictated by five methods and the tested plant fractions demonstrated a noteworthy antioxidant action.

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Evaluation of Secondary Metabolites and Antioxidant Activity of Water, Ethyl Acetate and Hexane Fractions from the Mangrove Young Leaves Sonneratia alba

Chemical Science International Journal ◽

10.9734/csji/2021/v30i230215 ◽

2021 ◽

pp. 23-32

Author(s):

Verly Dotulong ◽

Djuhria Wonggo ◽

Lita A. D. Y. Montolalu

Keyword(s):

Antioxidant Activity ◽

Secondary Metabolites ◽

Ethyl Acetate ◽

Secondary Metabolite ◽

Antioxidant Activities ◽

Extraction Methods ◽

Water Fraction ◽

Sonneratia Alba ◽

Dpph Method ◽

Young Leaves

The fractions from young leaves of mangrove Sonneratia alba was studied for its associated secondary metabolites and antioxidant activities. The objective of this study was to determine the secondary metabolite components and antioxidant activity of water, ethyl acetate, and hexane fractions of the young leaves of mangrove S. alba. The fraction was obtained from dry powder of young leaf S.alba using continuous fractionation of crude extracts. The crude extract was attained by 2 extraction methods (soxhlet and maceration) and 2 extraction solvents (methanol and ethanol). Secondary metabolites analyses were qualitatively conducted to detect the presence or absence of phenols, flavonoid, tannin, steroid, triterpenoid and alkaloid. Total phenols were measured using Folin Ciocalteau reagents and gallic acid standard curves whereas antioxidant activity were analyzed using DPPH method (1- 1-diphenil-2-picrihydrasil). Results showed that all fractions contained secondary metabolite components tested. The highest rendement was found in the water fraction fromsoxhletation extract with methanol (6.36±0.29%). The total phenol values were found the highest in the ethylacetatefraction from macerated extract with ethanol (352±9.77 mgGAE/g). Stronger antioxidant activity was also found in ethylacetate fraction as indicated by the small value of IC50 DPPH namely the ethylacetate fraction with soxhletation extract with ethanol (3.43±0.25 µg / mL). The results of this study indicate that the semipolar fraction (ethylacetate fraction) has more potential as a source of natural antioxidants.

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Determination of total phenolic and flavonoid contents of jackfruit peel and in vitro antiradical test

Food Research ◽

10.26656/fr.2017.5(1).350 ◽

2020 ◽

Vol 5 (1) ◽

pp. 84-90

Author(s):

Yamin ◽

Ruslin ◽

Mistriyani ◽

Sabarudin ◽

S. Ihsan ◽

...

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Colorimetric Method ◽

Ethyl Acetate Fraction ◽

Premature Aging ◽

Natural Antioxidant ◽

Aluminium Chloride ◽

Total Phenolic ◽

Phenolic And Flavonoid

Free radical is any molecular species that have unpaired free electrons in their outer orbital shell that make radicals highly reactive, resulting in pathogenesis conditions such as cellular injury, premature aging, cancer, hepatic disorders, neurodegenerative diseases, cardiovascular disease, and kidney disease. One source of natural antioxidant is jackfruit. The purpose of this research was to determine the phenolic and flavonoid contents in the extracts and fractions of jackfruit peel and their potential as antioxidants. Jackfruit peel powder was extracted from maceration. The total phenolic content was determined by the Folin-Ciocalteu method. Meanwhile, flavonoid content was determined using the aluminium chloride complex colorimetric method. Measurements of antioxidant activity were conducted using the 2,2-diphenyl-1-picrylhydrazyl (DPPH). The ethyl acetate fraction had high phenolic and flavonoid contents, which were 49.667±1.508 g GAE/100 g of sample and 70.199±0.374 g of quercertin equivalent/100 g. The ethyl acetate fraction had the strongest antioxidant activity with IC50 value of 4.539±0.201 µg/ mL and correlation value (R2 ) of 0.5881 for phenols and R2 of 0.7241 for flavonoids. Ethyl acetate fraction of jackfruit peel is very potential to be developed as a natural antioxidant and functional food.

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Chemical composition and antioxidant activity of Astragalus monspessulanus L. growing in semiarid areas of Algeria

Journal of the Serbian Chemical Society ◽

10.2298/jsc161212085b ◽

2018 ◽

Vol 83 (1) ◽

pp. 31-38 ◽

Cited By ~ 3

Author(s):

Sawsen Bourezzane ◽

Hamada Haba ◽

Christophe Long ◽

Mohammed Benkhaled

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Optical Rotation ◽

Radical Scavenging ◽

2D Nmr ◽

Test Method ◽

Phytochemical Study ◽

Butanol Extract ◽

Aerial Parts ◽

Nmr Techniques

This paper reports a phytochemical study of the aerial parts of Astragalus monspessulanus L. growing in Algeria. It deals with the isolation and structure elucidation of 13 known compounds. From the n-butanol extract, seven flavonoids 1?7, two saponins and one lignan were isolated. In addition, two phytosterols and one triterpenoid were isolated from the ethyl acetate extract. The structures of all the isolated products were determined by using 1D and 2D-NMR techniques, measurement of optical rotation, mass spectrometry ESI-MS and by comparison with literature data. Furthermore, the antioxidant activity of the n-butanol extract of the aerial parts of Astragalus monspessulanus L. was investigated using the DPPH radical scavenging and ferrous ion chelating assays. The n-butanol extract showed low (EC50 = 2.09?0.434 mg mL-1) to moderate (IC50 = 63.60?0.01 ?g mL-1) antioxidant activities depending on the test method.

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