Probiotics Efficacy on Oxidative Stress Values in Inflammatory Bowel Disease: A Randomized Double-Blinded Placebo-Controlled Pilot Study

2019 ◽  
Vol 19 (3) ◽  
pp. 373-381 ◽  
Author(s):  
Andrea Ballini ◽  
Luigi Santacroce ◽  
Stefania Cantore ◽  
Lucrezia Bottalico ◽  
Gianna Dipalma ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Test Group ◽  
Antioxidant Response ◽  
Control Group ◽  
Placebo Control ◽  
Bowel Diseases ◽  
Inflammatory Bowel ◽  
Double Blinded ◽  
To Receive ◽  
Bap Test

Background: Inflammatory bowel diseases (IBD), which include Crohn’s disease (CD) and ulcerative colitis (UC), are described as a chronic inflammation of the small intestine and colon, caused by a dysregulated immune response to host intestinal microbiota in genetically susceptible subjects. Objective: The aim of this study was to compare probiotic therapy versus placebo in Oxidative Stress Values and clinical features in patients affected by IBD. Method: Forty (40) patients previously diagnosed for IBD were recruited and randomized to receive probiotics (test group, n=20) or placebo (control group, n=20) administered for 90 days. Subjects in both the groups were assessed for overall oxidant ability (d-ROMs test) and for the antioxidant response (BAP test): data were reported at baseline, after 1 and 3 months. Additional data from anamnesis and haematological investigation were also reported during the study. Results: d-ROM assay clearly showed that the values observed in the test group were significantly improved, leading to oxidative stress values which are not pathological. The test group showed increasing BAP values, thus confirming the overall improvements of patients ‘health following administration of probiotics. Conclusion: Oral administration of the specific probiotics demonstrated its efficacy and safety on patients affected by IBD.

scholarly journals Probiotic Nutrition for Gastrointestinal Health Modulations in Inflammatory Bowel Disease: A Placebo-Controlled Clinical Study (P20-021-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Rajiv Saini
Keyword(s):  
Oxidative Stress ◽  
Test Group ◽  
Antioxidant Response ◽  
Control Group ◽  
Placebo Control ◽  
Gastrointestinal Health ◽  
Funding Sources ◽  
Bowel Diseases ◽  
Inflammatory Bowel ◽  
To Receive

Abstract Objectives Inflammatory bowel diseases (IBD), which include Crohn's disease (CD) and ulcerative colitis (UC), are described as a chronic inflammation of the small intestine and colon, caused by a dysregulated immune response to host intestinal microbiota in genetically susceptible subjects. The aim of this study was to compare probiotic therapy versus placebo in Oxidative Stress Values and clinical features in patients affected by IBD. Methods 40 patients previously diagnosed for IBD were recruited and randomized to receive probiotics (test group, n = 20) or placebo (control group, n = 20) administered for 90 days. Subjects in both the groups were assessed for overall oxidant ability (d-ROMs test) and for the antioxidant response (BAP test): data were reported at baseline, after 1 and 3 months. Additional data from anamnesis and haematological investigation were also reported during the study. Results d-ROM assay clearly showed that the values observed in the test group were significantly improved (436 ± 25 to 345 ± 29), leading to oxidative stress values, which are not pathological. The test group showed increasing BAP values (1740 ± 125 to 2150 ± 125), thus confirming the overall improvements of patient's health following administration of probiotics. Conclusions Oral administration of the multi strain probiotics demonstrated its efficacy and safety on patients affected by IBD. Funding Sources There is no funding source.

scholarly journals Supplementation of iron alone and combined with vitamins improves haematological status, erythrocyte membrane fluidity and oxidative stress in anaemic pregnant women

2010 ◽  
Vol 104 (11) ◽  
pp. 1655-1661 ◽  
Author(s):  
Ai Guo Ma ◽  
Evert G. Schouten ◽  
Yong Ye Sun ◽  
Fang Yang ◽  
Xiu Xia Han ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Pregnant Women ◽  
Membrane Fluidity ◽  
Erythrocyte Membrane ◽  
Ferrous Sulphate ◽  
Randomised Trial ◽  
Control Group ◽  
Placebo Control ◽  
Group I ◽  
Erythrocyte Membrane Fluidity

Pregnancy is a condition exhibiting increased susceptibility to oxidative stress, and Fe plays a central role in generating harmful oxygen species. The objective of the present study is to investigate the changes in haematological status, oxidative stress and erythrocyte membrane fluidity in anaemic pregnant women after Fe supplementation with and without combined vitamins. The study was a 2 months double-blind, randomised trial. Pregnant women (n 164) were allocated to four groups: group C was the placebo control group; group I was supplemented daily with 60 mg Fe (ferrous sulphate) daily; group IF was supplemented daily with Fe plus 400 μg folic acid; group IM was supplemented daily with Fe plus 2 mg retinol and 1 mg riboflavin, respectively. After the 2-month trial, Hb significantly increased by 15·8, 17·3 and 21·8 g/l, and ferritin by 2·8, 3·6 and 11·0 μg/l, in the I, IF and IM groups compared with placebo. Polarisation (ρ) and microviscosity (η) decreased significantly in other groups compared with placebo, indicating an increase in membrane fluidity. Significant decreases of ρ and η values compared with group C were 0·033 and 0·959 for group I, 0·037 and 1·074 for group IF and 0·064 and 1·865 for group IM, respectively. In addition, significant increases of glutathione peroxidase activities and decreases of malondialdehyde were shown in all treated groups, as well as increases of plasma retinol and urine riboflavin in group IM. The findings show that supplementation with Fe and particularly in combination with vitamins could improve the haematological status as well as oxidative stress and erythrocyte membrane fluidity.

Short-term Changes After Corticosteroid Injections Into the Normal Tendons of Rabbits: A Controlled Randomized Study

2019 ◽  
Vol 47 (3) ◽  
pp. 721-728 ◽  
Author(s):  
Kandir Genesio Innocenti Dinhane ◽  
Alexandre Leme Godoy-Santos ◽  
Alexandre Todorovic Fabro ◽  
Maria Regina Moretto ◽  
Igor Deprá ◽  
...  
Keyword(s):  
Single Injection ◽  
Corticosteroid Injection ◽  
Healing Process ◽  
Test Group ◽  
Control Group ◽  
Placebo Control ◽  
Short Term ◽  
Calcaneal Tendon ◽  
The Right ◽  
Corticosteroid Injections

Background: Corticosteroid injections in or around tendons for the treatment of athletic injuries are a common practice among orthopaedic surgeons and are apparently efficacious in the short term, although controversies persist related to local complications. Purpose: This study evaluated short-term (48 hours) biomechanical, biochemical, and histological alterations after a single injection of betamethasone into the normal tendons of rabbits. Study Design: Controlled laboratory study. Methods: A total of 72 New Zealand White rabbits were randomly divided into 2 groups: the test group—in which 36 animals underwent 1 intratendinous injection of betamethasone (1.4 mg / 0.2 mL) in the right calcaneal tendon; the control group—in which the right calcaneal tendon of 36 animals was injected with saline (placebo control group) and the left calcaneal tendon was left untreated for normal standards (normal control). Forty-eight hours later, animals were euthanized and tendons were harvested. Metalloproteinase (MMP1 and MMP2) and interleukin (IL1 and IL6) expression levels, biomechanical resistance (load × elongation parameters), and histomorphometry (hematoxylin and eosin and picrosirius red stains for collagen fibers, tenocytes, and inflammatory cells) were analyzed in the tendons. Results: The test group had a significant reduction in MMP2 expression as compared with the control groups ( P = .027). Regarding the other parameters, there were no additional significant differences between the groups. Conclusion: A single injection of corticosteroid into normal calcaneal tendons did not trigger acute local morphological, structural, or biomechanical injuries at 48 hours, but it did promote a significant decrease in MMP2 levels. Additional studies are needed with increased duration of follow-up, various doses, and multiple injections and in tendinopathic models. Clinical Relevance: Some previous studies demonstrated early structural changes in tendons after a single corticosteroid injection, which was not corroborated by the present study. Metalloproteinase decrease is usually associated with a reduction in collagen degradation, which would be protective for the healing process. More studies are necessary to confirm the possible beneficial effect of these results in the long term and for tendinopathies.

scholarly journals The Effects of Hyperbaric Oxygen at Different Pressures on Oxidative Stress and Antioxidant Status in Rats

Medicina ◽  
2019 ◽  
Vol 55 (5) ◽  
pp. 205 ◽  
Author(s):  
Şefika Körpınar ◽  
Hafize Uzun
Keyword(s):  
Oxidative Stress ◽  
Hyperbaric Oxygen ◽  
Antioxidant Status ◽  
Central Retinal Artery Occlusion ◽  
Retinal Artery Occlusion ◽  
Antioxidant Response ◽  
Control Group ◽  
Albino Rats ◽  
Normobaric Oxygen ◽  
Group I

Background: The optimal use of oxygen at greater than atmospheric pressures in any operational or therapeutic application (hyperbaric oxygen, HBO2) requires awareness of the fact that the beneficial effects of oxygen coexist with toxic effects depending on the pressure and duration of exposure. In this study, we aimed to investigate the effect of HBO2 therapy on oxidative stress and antioxidant status in commonly used protocol for acute HBO2 indications, such as carbon monoxide intoxication, central retinal artery occlusion, crush injury, gas gangrene, and to compare it with normobaric oxygen (NBO2) in healthy rats. Materials and Methods: Fifty-six male, young adult Wistar albino rats were randomly divided into seven groups and named as Group I through Group VII. Plasma malondialdehyde (MDA), superoxide dismutase (SOD), and erythrocyte glutathione (GSH) levels in control group were compared to the levels in other groups. Results: The increases in MDA levels and the decrease in SOD activities were statistically significant in HBO2 groups at the end of the first 24 h when compared to the control group, and the significant decrease in erythrocyte GSH level was only at 2.4 atmospheres absolute. Conclusions: The present study showed that pressure and frequency of exposure are important factors to consider when investigating HBO2-induced oxidative stress and antioxidant response.

Phase I, II and III Trials in Inflammatory Bowel Diseases: A Practical Guide for the Non-specialist

2020 ◽  
Vol 14 (5) ◽  
pp. 710-718 ◽  
Author(s):  
Ferdinando D’Amico ◽  
Cedric Baumann ◽  
Hélène Rousseau ◽  
Silvio Danese ◽  
Laurent Peyrin-Biroulet
Keyword(s):  
Clinical Trial ◽  
Data Analysis ◽  
Inflammatory Bowel Diseases ◽  
Control Group ◽  
Patient Stratification ◽  
Clinical Phase ◽  
New Drug ◽  
Bowel Diseases ◽  
Inflammatory Bowel ◽  
Design Type

Abstract In the last few decades several new molecules have been developed in the field of inflammatory bowel diseases. However, the process that leads to the approval and use of a new drug is very long, expensive and complex, consisting of various phases. There is a pre-clinical phase that is performed on animals and a clinical phase that is directed to humans. Each research phase aims to evaluate different aspects of the drug and involves a specific target group of subjects. In addition, many aspects must be considered in the evaluation of a clinical trial: randomization, presence of a control group, blind design, type of data analysis performed, and patient stratification. The objective of this review is to provide an overview of the clinical trial phases of a new drug in order to better understand and interpret their results.

scholarly journals Effect of Quercetin Monoglycosides on Oxidative Stress and Gut Microbiota Diversity in Mice with Dextran Sodium Sulphate-Induced Colitis

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Zhu Hong ◽  
Meiyu Piao
Keyword(s):  
Oxidative Stress ◽  
Body Weight Gain ◽  
Sodium Sulphate ◽  
The Body ◽  
Control Group ◽  
Simpson Index ◽  
Dextran Sodium Sulphate ◽  
Related Factors ◽  
Inflammatory Bowel ◽  
Quercetin Aglycone

The pathogenesis of inflammatory bowel disease (IBD) is linked to an intricate association of environmental, microbial, and host-related factors. This study examined the potential effects of dietary addition of two preparations from onion, one comprising quercetin aglycone alone (Q: 0.15% polyphenols, quercetin aglycone:quercetin monoglycosides, 98:2) and another comprising quercetin aglycone with monoglycosides (Q+MQ: 0.15% total polyphenols, quercetin aglycone:quercetin monoglycosides, 69:31), on dextran sodium sulphate- (DSS-) induced colitis in mice. The results revealed a significant decrease in the body weight gain of the mice with DSS-induced colitis, which was counteracted by the dietary Q or Q+MQ supplementation. Meanwhile, the oxidative stress indicated by myeloperoxidase (MPO), reduced glutathione (GSH), malondialdehyde (MDA), and serum nitrate (NO) concentrations was higher in mice with DSS-induced colitis than in the control group mice, but dietary Q or Q+MQ supplementation counteracted this trend. The colitis mice demonstrated reduced Chao1, angiotensin-converting enzyme (ACE), and Shannon indices and an increased Simpson index, but the colitis mice receiving dietary Q or Q+MQ exhibited higher Chao1, ACE, and Shannon indices and a reduced Simpson index. In conclusion, this research showed that even at a low dose, dietary Q or Q+MQ supplementation counteracts DSS-induced colitis in mice, indicating that Q or Q+MQ may be used as an adjuvant therapy for IBD patients.

Antioxidant potential of thyme extract: alleviation of N-nitrosodiethylamine-induced oxidative stress

2008 ◽  
Vol 27 (3) ◽  
pp. 215-221 ◽  
Author(s):  
P Rana ◽  
G Soni
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Test Group ◽  
Normal Diet ◽  
Lower Degree ◽  
Control Group ◽  
Albino Rats ◽  
Stress Induction ◽  
And Control

Protective role of thyme extract against N-nitrosodiethylamine (NDEA)-induced oxidative stress has been evaluated in albino rats. For this, one group of rats were fed diet supplemented with thyme extract (0.5%) and served as the test group, whereas animals of the other group fed on normal diet served as the control group. The rats were fed on respective diets for a period of 2 weeks after which stress was induced to half the animals of each group by i.p. administration of NDEA at 200 mg/kg body weight. Animals were killed 48 h post stress-induction period. Feed intake and body weight decreased significantly in both test and control groups, the effect being less in test group. Increase in osmotic fragility and in-vitro lipid peroxidation (LPO) on stress induction was of lower degree in the test group. NDEA toxicity was mainly reflected in liver as evidenced by increased activities of plasma aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase. The effect was of lower degree in test group as compared with that in the control group. Increase in urea levels observed following NDEA administration was also of lower degree in test groups. Blood glutathione (GSH) levels increased more so in test group compared with control group on stress induction. The activities of superoxide dismutase (SOD), peroxidase (Px), and catalase (CAT) activities decreased significantly on stress induction in erythrocytes. LPO increased in all the tissues through varying degree, and the increase was appreciably of lower degree in test group. The activity of SOD increased significantly in both test and control group on stress induction, whereas activities of Px and CAT decreased following NDEA treatment, and the effects were of lower degree in test group. Thus, supplementation of diet with thyme extract can improve antioxygenic potential and hence help to prevent oxidative stress.

scholarly journals Effects of Probiotic Supplementation on Immune Response in Soldiers: A Randomized, Double-Blinded, Placebo-Controlled Trial

2020 ◽  
Vol 18 (3) ◽  
Author(s):  
Mahtab Noorifard ◽  
Elahe Ebrahimi ◽  
Arasb Dabbagh Moghaddam ◽  
Zatollah Asemi ◽  
Ramin Hamidi Farahani ◽  
...  
Keyword(s):  
Immune Response ◽  
Immune System ◽  
Controlled Trial ◽  
Test Group ◽  
Bacillus Coagulans ◽  
Serum Lactate ◽  
Control Group ◽  
Probiotic Supplementation ◽  
Double Blinded ◽  
The Military

Background: Athletic soldiers undergo strenuous and high-endurance training to prepare for competition. Stress and deprivation of proper nutrition can weaken the immune system in athletic soldiers. Therefore, enhancing the immune system function in addition to enhancing the performance of athletic soldiers can lead to a reduction in health costs for the military. Objectives: In this study, the effects of probiotic supplementation on the immune response of soldiers were assessed. Methods: In a randomized, double-blinded, placebo-controlled trial, 42 athletic soldiers were randomly divided into two groups of 21 members. Participants in the probiotic group daily received one glass of fruit juice containing Bacillus coagulans (2 × 109 CFU/g). The control group consumed the placebo at the same time. At the beginning and end of the study, as well as at specific times, IgA, CD4, lactate, and urea were measured in blood samples of all participants. Results: Probiotic supplementation increased the concentration of IgA (+115.7 ± 28.3 vs. -108.3 ± 25.7 mg/dL, P < 0.001) and CD4 levels (+129.1 ± 42.6 vs. -54.5 ± 114.6 cells/µL, P < 0.001) after three months in the test group. In addition, a significant decrease was seen in serum lactate (-5.7 ± 10.1 vs. +10.1 ± 7.4. mg/L, P < 0.001) and urea (-9.9 ± 6.7 vs. +2.3 ± 5.6 mg/dL, P < 0.001) concentrations following probiotic supplementation compared to the placebo. Conclusions: Probiotic consumption for 12 weeks has a positive effect on the immune response of soldiers.

scholarly journals The Prevalence of Adherent-Invasive Escherichia coli and Its Association With Inflammatory Bowel Diseases: A Systematic Review and Meta-Analysis

2021 ◽  
Vol 8 ◽  
Author(s):  
Razie Kamali Dolatabadi ◽  
Awat Feizi ◽  
Mehrdad Halaji ◽  
Hossein Fazeli ◽  
Peyman Adibi
Keyword(s):  
Escherichia Coli ◽  
Systematic Review ◽  
Inflammatory Bowel Diseases ◽  
Meta Analysis ◽  
Control Group ◽  
Pooled Prevalence ◽  
Bowel Diseases ◽  
Phylogenetic Grouping ◽  
Inflammatory Bowel ◽  
And Control

Inflammatory bowel diseases (IBD), including Crohn's disease (CD) and ulcerative colitis (UC), are known as chronic gastrointestinal inflammatory disorders. The present systematic review and meta analysis was conducted to estimate the prevalence of adherent-invasive Escherichia coli (AIEC) isolates and their phylogenetic grouping among IBD patients compared with the controls. A systematic literature search was conducted among published papers by international authors until April 30, 2020 in Web of Science, Scopus, EMBASE, and PubMed databases. The pooled prevalence of AIEC isolates and their phylogenetic grouping among IBD patients as well as in controls was estimated using fixed or random effects models. Furthermore, for estimating the association of colonization by AIEC with IBD, odds ratio along with 95% confidence interval was reported. A total of 205 articles retrieved by the initial search of databases, 13 case–control studies met the eligibility criteria for inclusion in the meta analysis. There were 465 IBD cases (348 CD and 117 UC) and 307 controls. The pooled prevalence of AIEC isolates were 28% (95% CI: 18–39%), 29% (95% CI: 20–40%), 13% (95% CI: 1–30%), and 9% (95% CI: 3–19%), respectively among IBD, CD, UC, and control group, respectively. Our results revealed that the most frequent AIEC phylogroup in the IBD, CD, and control groups was B2. Fixed-effects meta analysis showed that colonization of AIEC is significantly associated with IBD (OR: 2.93; 95% CI: 1.90–4.52; P &lt; 0.001) and CD (OR: 3.07; 95% CI: 1.99–4.74; P &lt; 0.001), but not with UC (OR: 2.29; 95% CI: 0.81–6.51; P = 0.11). In summary, this meta analysis revealed that colonization by AIEC is more frequent in IBD and is associated with IBD (CD and UC). Our results suggested that the affects of IBD in patients colonized with the AIEC pathovar is not random, it is in fact a specific disease-related pathovar.

scholarly journals Formulation, Optimization and Pharmacodynamic Studies of Pioglitazone HCl Solid Lipid Nanoparticles

2021 ◽  
pp. 329-341
Author(s):  
Y. Indira Muzib ◽  
E. Ramya ◽  
Y. R. Ambedkar
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Reference Group ◽  
Test Group ◽  
Tween 80 ◽  
Lipid Nanoparticles ◽  
Control Group ◽  
Albino Rats ◽  
Placebo Control ◽  
Solid Lipid ◽  
Group I

Pioglitazone HCl is an oral anti-diabetic agent used for the treatment of diabetes mellitus type II. The aim of the present work is to evaluate the pharmacodynamic activity of solid lipid nanoparticles of pioglitazone HCL prepared by using solvent injection technique and to compare with the control and test group. Among all the formulations, F5 was found to possess highest in-vitro drug release within 24 hrs i.e., 95.02±1.26%. The in vivo studies were performed using male albino rats of wistar strain (150-200g). Rats were divided in to five groups (n=6), group-I normal, group-II diabetes control, group-III   placebo control, group-IV reference, group-V test group. Diabetes was induced by streptazocin (60 mg/kg) by intraperitonial route. The reference group was treated with marketed tablet of pioglitazone HCL, test groups were treated with SLNs suspended in 0.1% Tween 80 and given to animals through oral gavages. Blood samples were collected by retro-orbital puncture before treatment, and after treatment at time intervals 0, 2, 4, 6, 8, 10, 12 and 24h in anti-coagulated vials. Parameters like glucose, tri glycerides (TG), total cholesterol (TC) and HDL-C were estimated by calorimetric method.  Diabetes induced rats showed elevated levels of glucose, TG, TC and reduced HDL. The oral administration of drug loaded SLNs in 0.1% Tween 80 solution showed reduced levels of glucose, TG and elevated levels of HDL-C and slightly reduced levels of TG in 24 h where as the marketed tablet showed reduced levels of glucose, TG and TC up-to 12 h and in 24thh  the glucose levels get elevated. Thus the optimized SLNs showed prolonged activity.

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