MicroRNA Expression Levels in Patients with Hashimoto Thyroiditis: A Single Centre Study

2020 ◽  
Vol 20 ◽  
Author(s):  
Hale Onder Yilmaz ◽  
Alper Han Cebi ◽  
Mustafa Kocak ◽  
Halil Onder Ersoz ◽  
Mevlit Ikbal
Keyword(s):  
Hashimoto Thyroiditis ◽  
Healthy Controls ◽  
Blood Samples ◽  
Expression Levels ◽  
Diagnosis Group ◽  
Significant Difference ◽  
Single Centre Study ◽  
Polymerase Chain ◽  
Moderate Positive Correlation

Objective: To determine the circulatory miRNA expression levels in patients with Hashimoto thyroiditis (HT) at the time of diagnosis and follow-up period compared with healthy controls. Methods: We collected blood samples from 34 patients with HT (4 males and 30 females) at the time of first diagnosis (Group P) and euthyroid period (Group E). Thirty-three healthy controls (Group H) blood samples were also included in the study. Expression levels of five different circulating miRNAs (miR-22, miR-141, miR-155, miR-375, miR-451) were evaluated using real-time polymerase chain reaction. Results: There was a significant difference in miR-375 levels between the P group and the H. Also, for miR-451, there was a significant difference between the P and E groups. Finally, there was a moderate positive correlation between thyroidstimulating hormone values and miR-22 expression levels for the P group. Conclusion: miRNAs have important roles at all stages of the diseases. More studies must be performed in all thyroid diseases and autoimmune diseases, including HT.

scholarly journals Pyrimidine 5’Nucleotidase I and II Activities in Acute Leukaemias

2004 ◽  
Vol 47 (2) ◽  
pp. 129-131 ◽  
Author(s):  
Haci Kemal Erdemli ◽  
Bahattin Adam ◽  
Nüket Bavbek
Keyword(s):  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Mononuclear Cells ◽  
Control Group ◽  
Healthy Controls ◽  
Blood Samples ◽  
Significant Difference ◽  
Peripheral Mononuclear Cells ◽  
Design And Methods

Bacground and objective: Pyrimidine 5’nucleotidase I and II activities of peripheral mononuclear cells were studied to evaluate their role in diagnosis, assessment of therapy and follow up of remission in acute leukaemias. Design and methods: Blood samples were obtained from 40 untreated patients with acute lymphoblastic and myeloid leukaemia and 40 healthy controls, before the therapy and after remission. The correlation between the activity of the enzymes and the efficacy of therapy were established. The enzyme activities were measured by High-Performance Liquid Chromatography (HPLC), using the method described by Amici. For statistical analysis, Mann-Whitney U, Kruskal-Wallis and Wilcoxon methods were used. Results: Before the therapy, Pyrimidine 5’nucleotidase I levels in the leukaemic group were found to be significantly elevated when compared to the control group (p<0.001). Also Pyrimidine 5’nucleotidase II levels were significantly elevated before the therapy and during remission (p<0.02 and p<0.001 respectively). The isoenzyme activities were compared in patients who were in remission, who did not respond to therapy and in patients who died during the therapy, but no significant difference was found. Interpretation and conclusions: We concluded that, Pyrimidine 5’nucleotidase I and II activities can be used as markers for diagnosis and follow up of remission in patients with acute leukaemia. But, they can not have predictive value for prognosis.

scholarly journals MON-LB82 Plasma Expression Level of MiRNA -21 Is Related With the Presence of Papillary Thyroid Cancer

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Birute Zilaitiene ◽  
Aiste Kondrotiene ◽  
Daina Pamedytyte ◽  
Vaida Simanaviciene ◽  
Dalia Dauksiene ◽  
...  
Keyword(s):  
Thyroid Cancer ◽  
Healthy Volunteers ◽  
Papillary Thyroid Cancer ◽  
Diagnostic Value ◽  
Healthy Controls ◽  
Papillary Thyroid ◽  
Expression Levels ◽  
Significant Difference ◽  
Baseline Levels ◽  
Benign Nodules

Abstract Introduction.: There is no effective and reliable biomarker to distinguish benign thyroid nodules (BTN) from papillary thyroid carcinomas (PTC). In this study we analyzed a set of four miRNA molecules in plasma of patients with papillary thyroid cancer, benign nodules and healthy controls to identify miRNA molecules that may be markers of PTC.Aim.: We aimed to investigate the dysregulation of plasma miRNAs in PTC and evaluate the diagnostic value for differentiation of PTC from BTN. Methods.: The expression levels of 4 miRNAs (miR-221, miR-222, miR-146b, miR-21) were measured in 48 PTC patients before thyroidectomy and again after thyroidectomy in a subgroup of 36 patients. Preoperative and postoperative plasma miRNA expression levels were compared with baseline levels established in plasma from the heathy controls group (N=57) and patients with BTN (N=22). MicroRNA-222 and miR-146b, miR-221, miR-21 were included in a panel because they all reportedly were overexpressed in PTC compared to benign nodules or normal thyroid tissue.Results.: Compared with baseline levels in the healthy controls group, miR-221, miR-222, miR-146b, miR-21 levels were significantly higher in the preoperative PTC group (P &lt;0.0001, P=0.002, P=0.028, P =0.021, respectively). A significant reduction in miR-21 expression was observed in postoperative PTC patients. MiR-21 decreased by 5.98-fold (P=0.046) in post- operative samples compared with preoperative samples in the PTC patients.In comparison MiRNRs expression levels in BTN group with healthy controls, miR-221, miR-21 expression levels were significantly higher in the BTN group (P=0.003, P=0.048, respectively). No significant difference was observed between the preoperative PTC group and the preoperative BTN group with regard to the expression of these four miRNA’s. Conclusions: The expression levels of miR-222, miR-146b in plasma were significantly higher in patients who had PTC than in healthy volunteers, whereas levels of miR-221, miR-21 in plasma were significantly higher in patients who had either PTC or BTN before thyroidectomy than in healthy volunteers. Furthermore, miR-21 showed a significant reduction of expression levels after thyroidectomy in PTC patients. However, value of these four miRNAs is still limited in differential diagnosis of PTC and benign nodules.

Study of the HLA-DPβ1 Locus by the Polymerase Chain Reaction Technique in Patients with Hodgkin's Disease

1993 ◽  
Vol 79 (2) ◽  
pp. 133-136 ◽  
Author(s):  
Guseppe Pellegris ◽  
Claudia Lombardo ◽  
Annelisa Cantoni ◽  
Liliana Devizzi ◽  
Monica Balzarotti
Keyword(s):  
Polymerase Chain Reaction ◽  
Hodgkin's Disease ◽  
Hodgkin’S Disease ◽  
Polymerase Chain Reaction Method ◽  
Healthy Controls ◽  
Chain Reaction ◽  
Reaction Method ◽  
Significant Difference ◽  
Polymerase Chain

Background A number of reports have studied associations between Hodgkin's disease and HLA. Some of them established correlation between several antigens and Hodgkin's disease, and others found no correlations. Methods The HLA DP locus was determined by the polymerase chain reaction method in 31 Hodgkin's disease patients and 58 healthy controls. Results No significant difference between patients and controls was noted. Conclusions Further investigations are needed to confirm the hypothesis of a possible role of the HLA complex as one of the factors involved in Hodgkin's disease.

Detection of Subclinical Systemic Disease in Primary CNS Lymphoma by Polymerase Chain Reaction of the Rearranged Immunoglobulin Heavy-Chain Genes

2006 ◽  
Vol 24 (29) ◽  
pp. 4754-4757 ◽  
Author(s):  
Kristoph Jahnke ◽  
Michael Hummel ◽  
Agnieszka Korfel ◽  
Thomas Burmeister ◽  
Philipp Kiewe ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Polymerase Chain Reaction ◽  
Systemic Disease ◽  
Primary Cns Lymphoma ◽  
Cns Lymphoma ◽  
Chain Reaction ◽  
Blood Samples ◽  
Pcr Products ◽  
Polymerase Chain

Purpose To search for subclinical systemic disease in bone marrow and peripheral blood in patients with primary CNS lymphoma (PCNSL) to elucidate whether extracerebral relapse may represent a sequel of initial occult systemic disease rather than true extracerebral spread. Patients and Methods Bone marrow and peripheral-blood specimens of 24 PCNSL patients were examined using polymerase chain reaction (PCR) for analysis of clonally rearranged immunoglobulin heavy-chain (IgH) genes. Results Identical dominant PCR products were found in bone marrow aspirates, blood samples, and tumor biopsy specimens of two patients, indicating that the same tumor cell population is present in the CNS and in extracerebral sites. Follow-up IgH PCR performed in one of these patients in complete remission 24 months after diagnosis yielded a persistent monoclonal product in the blood. An oligoclonal IgH rearrangement pattern was found in the tumor specimen of two other patients, whereas bone marrow and blood samples demonstrated the same dominant PCR products. Follow-up PCR showed a persistent monoclonal amplificate in blood in one of these patients 27 months after diagnosis. Conclusion It could be demonstrated for the first time that subclinical systemic disease can be present in PCNSL patients at initial diagnosis. Our findings may have an impact on the understanding of PCNSL pathogenesis and the extent of staging and treatment.

Peripheral blood microRNAs expression profile: A fingerprint for metastatic prostate cancer.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. e22171-e22171
Author(s):  
Vanessa Medina Villaamil ◽  
Guadalupe Aparicio ◽  
Isabel Santamarina Cainzos ◽  
Francisco Gomez-Veiga ◽  
Manuel Valladares Ayerbes ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Metastatic Prostate Cancer ◽  
Prostate Cancer Cell ◽  
Healthy Controls ◽  
Circulating Mirnas ◽  
Blood Samples ◽  
Expression Levels ◽  
Circulating Markers ◽  
Hormone Refractory ◽  
Level Of Significance

e22171 Background: We previously have studied differentially microRNA expression levels related to hormone refractory and sensitive prostate cancer cell lines (VCap and LNCap respectively). Now, we investigated circulating miRNAs differentially expressed between metastatic prostate adenocarcinomas (mPA) and healthy controls (HC) that may serve as novel diagnostic and/or prognosis markers. Methods: Using SYBR-green-based custom microRNA RT-qPCR arrays technology (Exiqon), we compared the expression levels of miRNAs in blood samples from 18 HC and 48 mPA. Results: Among a panel of 92 candidates we found 41 underexpressed (UE) and 8 overexpressed (OE) miRNAs. The level of significance was p<0.05. Conclusions: Among the UE miRNAs are miR-203 and 129 which regulate a cohort of metastatic genes. Among the OE is miR-200a, a member of miR-200 family which directly targeted β-catenin mRNA and miR-135b which induce invasion and distant metastasis. The knowledge of miRNAs involved in mPA progression could provide us a profile that could identify patients for adjuvant therapy or even propose new target drugs to avoid disease progression or treat mPA. Our group is actively involved in the study of selected OE miRNAs for their potential as circulating markers for mPA. [Table: see text]

scholarly journals The Efficacy of Chest X-Ray for the Diagnosis and Follow-up in Young Adult COVID-19 Patients with Mild Dyspnea No Comorbid Diseases

2021 ◽  
Author(s):  
ferhat cuce ◽  
deniz dogan ◽  
ugur bozlar ◽  
mustafa tasar ◽  
ervin gjoni ◽  
...  
Keyword(s):  
Young Adult ◽  
X Ray ◽  
Early Admission ◽  
Significant Difference ◽  
Chest X Ray ◽  
Polymerase Chain ◽  
Comorbid Diseases ◽  
Time Of Admission ◽  
Hospital Stay Duration

Purpose: We investigated whether Chest X-Ray (CXR) could replace CT modality in the diagnosis and during the treatment of young adult COVID-19 patients with mild dyspnea with no comorbid diseases. Materials and Method: This retrospective study involved an examination of the records of a total of 956 patients hospitalized between March 1 and May 15, 2020. The study included a total of 64 patients, aged 21–60 years with mild dyspnea with no comorbid diseases and with COVID-19 infection confirmed by a polymerase chain reaction, who underwent a CXR at admission and CT imaging within 24 hours. The first CXR and CT images at the time of admission were evaluated in terms of lesions and localization. The clinical-radiological course of the diseases with CXR were also statistically evaluated. Results: CT was normal in 18/64 (28.2%) patients, all of whom also had normal CXR. The rest of the patients 46/64 (71.8%) with an abnormal CT, the CXR was normal in 18/46 (39.1%) and abnormal in 28/46 (60.9%). The time between the onset of complaints and admission to the hospital in patients with abnormal and normal CXR was 3.5 ± 2.3 days and 2.1 ± 1.1, which was statistically significant (p = 0.004). The hospital stay duration of the patient with abnormal and normal CXR was 9.6 ± 3.5 and 9.5 ± 3.4 (p=0.928), respectively, and was not statistically significant. Conclusion: CXR could be used in the diagnosis and follow-up of young adult COVID 19 patients with mild dyspnea no comorbid disease. In the case of early admission to the hospital, there is not a significant difference between using CXR or CT in the management of these patients. Therefore, the use of CXR in these patients groups will reduce the burden of CT units in pandemic conditions with limited resources.

scholarly journals Molecular detection of Babesia bovis in cattle in Al-Qadisiyah province

2017 ◽  
Vol 40 (2) ◽  
pp. 155-158
Author(s):  
Noaman N. A,aiz
Keyword(s):  
Polymerase Chain Reaction ◽  
Infection Rate ◽  
Molecular Detection ◽  
Babesia Bovis ◽  
Chain Reaction ◽  
Adult Cattle ◽  
Blood Samples ◽  
Significant Difference ◽  
Polymerase Chain ◽  
Age And Sex

     This study aim to determine Babesia bovis infection in cattle based on genetic methods. A total of 96 blood samples were collected from alive and slaughtered cattle from different areas in addition to the abattoir of Al-Qadisiyah province from December 2013 to August 2014. Real time polymerase chain reaction (RT.PCR) technique was used to detect the presence of the protozoan with the effect of animal's age and sex in the infection rate 47.91 % (46/96) of examined cattle were given positive result to B. bovis infection. The highest infections were shown among the adult cattle (≥1 year), while there was non-significant difference (P>0.05) in the infection rate according to the sex. So the most cattle in Al-Qadisiyah province appear to be bearing the infection predominantly as a carrier hosts.

scholarly journals Double venipuncture is not required for adequate S-100B determination in melanoma patients

BioTechniques ◽  
2020 ◽  
Vol 69 (5) ◽  
pp. 371-378
Author(s):  
Samantha Damude ◽  
Anneke C Muller Kobold ◽  
Esther Bastiaannet ◽  
Schelto Kruijff ◽  
Harald J Hoekstra ◽  
...  
Keyword(s):  
Cancer Staging ◽  
Stage Iii ◽  
Tube System ◽  
Serum Samples ◽  
Blood Samples ◽  
Significant Difference ◽  
Melanoma Patients ◽  
The Mean ◽  
Stage Iii Melanoma

S-100B is used in melanoma follow-up. This serum biomarker is also present in adipocytes; therefore, subcutaneous adipocytes trapped in the needle before performing a venipuncture could contaminate the serum. The aim was to study the influence of adipocyte contamination on blood samples used for S-100B analysis, possibly resulting in falsely elevated S-100B values. A total of 294 serum samples were collected from 147 American Joint Committee on Cancer staging stage III melanoma patients. The mean difference between the first (dummy) and second tubes was 0.003 μg/l (p = 0.077), with a decrease in the second tube. Compared with the second tube, the S-100B level was higher in the first tube in 33.3% of the samples, equal in 36.8% of the samples and lower in 29.9% of the samples. No significant difference between the two consecutively drawn tubes was found. There seems to be no necessity of implementing a dummy tube system for accurate S-100B determination in melanoma patients.

Treatment and outcomes of polymerase chain reaction-confirmed early syphilis

Sexual Health ◽  
2015 ◽  
Vol 12 (6) ◽  
pp. 506 ◽  
Author(s):  
Phillip J. Read ◽  
Rebecca Guy ◽  
Neisha Jeoffreys ◽  
David Baker ◽  
Matthew Shields ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Standard Therapy ◽  
Significant Proportion ◽  
Hiv Positive ◽  
Hiv Status ◽  
Chain Reaction ◽  
Serological Tests ◽  
Significant Difference ◽  
Polymerase Chain

Background Syphilis is resurgent among gay and bisexual men (GBM) despite effective treatment and widely available diagnostic serology. The polymerase chain reaction assay for Treponema pallidum (TP-PCR) is available, but little is known about the clinical features and outcomes for patients testing positive by TP-PCR. Methods: Clinical data were collected from four medical practices for patients recording a positive TP-PCR result between 2004 and 2011. Demographic, serological, treatment and reinfection details were obtained. Results were stratified by HIV status and whether treatment conformed to international guidelines. Results: 220 patients were positive for TP-PCR, of whom 92% were GBM. Seventeen (8.1%) were positive by TP-PCR before seroconversion. Almost one-third (32.1%) received treatment beyond that recommended in guidelines, and this was associated with HIV status (40.3% HIV positive vs 22.4% HIV negative, P < 0.01). All but one patient with adequate follow up achieved serological cure. There was no significant difference in time to serological cure between the groups receiving standard therapy or enhanced therapy (95 vs 108 days; P = 0.67) or between HIV positive and negative patients (93 vs 104 days, P = 0.06). Nineteen patients were reinfected during follow up. Conclusion: TP-PCR aids early diagnosis of syphilis and may be reactive before conventional serological tests. Treatment outcomes for TP-PCR-positive early infection were excellent, but a significant proportion of patients received non-standard therapy. Expanded use of syphilis PCR testing in at-risk populations is recommended since early identification and treatment is likely to be important in controlling the current epidemic in GBM.

The Serum Level of Agalactosyl IgG in Rheumatoid Arthritis Patients Treated with Methotrexate

1996 ◽  
Vol 9 (1) ◽  
pp. 19-22
Author(s):  
J. K. Lacki ◽  
U. Mackiewicz ◽  
S. Mackiewicz ◽  
W. Muller
Keyword(s):  
Rheumatoid Arthritis ◽  
Serum Level ◽  
Nonsteroidal Antiinflammatory Drugs ◽  
Healthy Controls ◽  
Disease Course ◽  
Antiinflammatory Drugs ◽  
Significant Difference ◽  
One Year ◽  
Agalactosyl Igg

To verify the hypothesis that methotrexate may affect the serum level of agalactosyl IgG (IgG[0]) we followed the changes in IgG galactosylation patterns in a cohort of rheumatoid arthritis patients treated with either methotrexate (MTX) or nonsteroidal antiinflammatory drugs (NSAID). The average values of IgG[0] in RA patients at the beginning of the observation were significantly higher as compared to healthy controls (0.45 ± 0.39 vs. −0.03 ± 0.09, p<0.05). The findings of IgG[0] after one-year follow-up were also higher as compared to healthy controls (0.38 ± 0.39 vs. −0.03 ± 0.09, p<0.05). We did not notice any statistically significant difference in IgG[0] between MTX and NSAID treated patients at the beginning of the study (0.49 ± 0.42 vs. 0.42 ± 0.38, NS). However, during one-year MTX treatment IgG[0] significantly dropped (0.49 ± 0.42 vs. 0.25 ± 0.24, p<0.01). We did not establish any fluctuation in IgG[0] in the group of patients treated with NSAID (0.42 ± 0.38 vs. 0.46 ± 0.45, NS). The data thus far obtained suggest that IgG[0] may serve as an indicator for the disease course in patients with RA. Secondly, the clinical improvement and IgG[0] decrease after methotrexate implies, that the immunoregulatory abnormality in RA may be susceptible to correction by immunotherapy.

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