Development, Characterization and Pharmacological Evaluation of Anti-blemish Cream Containing Herbal Oils

2020 ◽  
Vol 14 ◽  
Author(s):  
Sathiya Ramu ◽  
Komala Mahadevappa ◽  
Radhika Muniyappa Narayanaswamy ◽  
Devanand Kamnoore ◽  
Ramya Lingutla ◽  
...  
Keyword(s):  
Human Subjects ◽  
Topical Administration ◽  
Water Type ◽  
Black Cumin ◽  
Therapeutic Value ◽  
Oil In Water ◽  
Before And After ◽  
Oecd Guideline ◽  
Chronic Use ◽  
Viscosity Stability

Background: Many topical agents are available in the market which interfere with pigmentation process at different levels. They are often known to cause side effects ranging from irritation to tumor over chronic use. Objective: The present study was designed to develop and characterize an antiblemish cream containing herbal oils. Methods: A herbal cream was formulated using dill, nagarmotha and black cumin oil and subjected to evaluation of its antiblemish potential against stress augmented UV-B rays induced hyperpigmentation. Topical oil in water type of cream containing 2%, 4% and 6% of each oil was formulated using herbal oils. The formulated cream was characterized for solubility, pH, particle size, grittiness, viscosity, stability, phase separation, shelf life and spreadability, and found to be stable. Acute dermal toxicity was carried out individually for dill, nagarmotha and black cumin oil according to OECD guideline 402. Hyperpigmentation was induced in all the experimental animals by stress augmented UV-B irradiation method. The animals were treated for 30 days (twice daily) with standard and test formulations by topical administration, whereas the disease group was left untreated. The skin of the animals were subjected to photographical study as well as grading for pigmentation and irritation before and after treatment. After treatment period, the serum antioxidant levels were estimated and histopathology, histochemical studies of skin were performed. Results: The animals treated with test formulations containing 2%, 4%, 6% of herbal oil showed significant improvement from pigmentation compared to disease control as it is evident in photographical biochemical, histopathological and histochemical studies. Conclusion: Thus, it was concluded that the developed antiblemish cream containing herbal oils possess significant antiblemish potential. This study necessitates further evaluations in human subjects as it could have highly positive therapeutic value in the treatment of hyperpigmentation.

scholarly journals Response in Growth, Scute Development, and Whole-Body Ion Composition of Acipenser fulvescens Reared in Water of Differing Chemistries

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1419
Author(s):  
Janet Genz ◽  
Rachael Hicks
Keyword(s):  
River Water ◽  
Growth And Development ◽  
Lake Sturgeon ◽  
Whole Body ◽  
Acipenser Fulvescens ◽  
Water Type ◽  
Ion Composition ◽  
Ion Concentrations ◽  
Before And After ◽  
Yolk Absorption

In fishes, environmental ion availability can have substantial effects on growth and development. This study examined the development of Lake Sturgeon in response to the varying environmental ion availability that they experience as part of a conservation stocking program. We reared sturgeon in natural water from the Coosa River, which had higher concentrations of Mg2+, Na+, and Zn2+ than standard hatchery conditions, while [Ca2+] at the Warm Springs National Fish Hatchery was 2× higher than in the Coosa River. Eggs were hatched in each water type and the larvae were sampled at time points before and after yolk absorption during the first 8 weeks of development. Total length and weight in WSNFH larvae were significantly higher than larvae in Coosa River water starting at 8 dph, indicating that growth was dependent on the different environmental ion levels. Concentrations of the ions of interest were also determined for whole-body acid digests of the exposed Lake Sturgeon. We found that Lake Sturgeon reared in Coosa River water had significantly higher magnesium and zinc than Lake Sturgeon reared in WSNFH water (p < 0.05), while calcium was significantly higher in WSNFH than Coosa River water. This difference shows that different environmental ion concentrations also impact the overall development of larval Lake Sturgeon.

Effects of a Low-Element Challenge Course on Abstinence Self-Efficacy and Group Cohesion

2011 ◽  
Vol 22 (2) ◽  
pp. 151-158 ◽  
Author(s):  
Jamie M. Clem ◽  
Thomas E. Smith ◽  
Kristin V. Richards
Keyword(s):  
Outcome Measures ◽  
Self Efficacy ◽  
Group Cohesion ◽  
Future Research ◽  
Chemically Dependent ◽  
Challenge Course ◽  
Self Confidence ◽  
Therapeutic Value ◽  
Before And After ◽  
Group Unity

Substance abuse researchers identify self-efficacy and group cohesion as important components in alcohol and other drug-dependency treatment. Objectives: The purpose of this single-group, pretest–posttest study is to explore the therapeutic value of a challenge course intervention on the self-efficacy and group cohesion of nine chemically dependent, adult females. Methods: Data were collected using two validated outcome measures administered before and after the intervention. Focus groups provided insight into the experiences of participants and were examined using manifest and latent theme analysis. Results: Findings indicate statistically significant improvements on both outcome measures with medium to large effect sizes. Several themes were identified including group unity, trust, interpersonal growth, and self-confidence. Conclusions: Suggestions for practice and future research are provided.

Therapeutic Management of Chronic Suppurative Otitis Media with Otic Drops

1993 ◽  
Vol 109 (1) ◽  
pp. 77-82 ◽  
Author(s):  
David O. Merifield ◽  
Nancy J. Parker ◽  
Nannette C. Nicholson
Keyword(s):  
Otitis Media ◽  
Human Subjects ◽  
Bone Conduction ◽  
Chronic Suppurative Otitis Media ◽  
Suppurative Otitis Media ◽  
Hearing Sensitivity ◽  
Significant Difference ◽  
Before And After ◽  
Ventilation Tubes ◽  
Bone Conduction Hearing

The use of potentially ototoxic topical drugs is controversial. Few experimental reports of audiometric data from human subjects exist. The purpose of this study is to determine if a significant difference between bone conduction hearing sensitivity before and after otic drop treatment for children with chronic suppurative otitis media and patent ventilation tubes exists. A statistical analysis of sensorineural threshold status after treatment of chronic suppurative otitis media with otic drops is presented.

scholarly journals Fibreoptic Bronchoscopy in the Critically Ill: A Prospective Study of its Diagnostic and Therapeutic Value

1992 ◽  
Vol 20 (4) ◽  
pp. 464-469 ◽  
Author(s):  
R. Bellomo ◽  
E. Tai ◽  
G. Parkin
Keyword(s):  
Prospective Study ◽  
Critically Ill ◽  
Lower Lobe ◽  
Major Complication ◽  
Diagnostic Value ◽  
Fibreoptic Bronchoscopy ◽  
Clinical Assessments ◽  
Therapeutic Value ◽  
Before And After ◽  
A Prospective Study

Aim A prospective study was undertaken to assess the diagnostic value and therapeutic usefulness of fibreoptic bronchoscopy in the critically ill. Method Fifty-six bronchoscopies were performed in fifty patients. Biochemical, radiological, microbiological and clinical assessments were made before and after each procedure. Results Eighteen fibreoptic bronchoscopies were performed for therapeutic indications (32.1%) of which ten (55.6%) yielded a useful outcome. Thirty-eight bronchoscopies were for diagnostic purposes (67.8%) of which 22 (5 7.9%) were clinically useful. Broncho-alveolar lavage was performed in twenty-eight cases (50%) and it led to a clinically useful diagnosis in 17 (60.7%). There was no major complication. A subgroup of patients was defined (persistent left lower lobe collapse or consolidation following thoracic or abdominal surgery) in whom fibreoptic bronchoscopy usually did not yield a useful outcome. Conclusion The use of fibreoptic bronchoscopy in the Intensive Care Unit, in combination with the technique of broncho-alveolar lavage, results in a clinically useful outcome in the majority of cases. Fibreoptic bronchoscopy is an effective and safe diagnostic and therapeutic tool in critically ill patients.

scholarly journals Determination of plasma glucose enrichment in human subjects after primed, constant infusions of 13C‐glycine before and after dietary vitamin B‐6 restriction

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Vanessa Rodrigues Silva ◽  
Yvonne Lamers ◽  
Maria Ralat ◽  
Lesa Gilbert ◽  
Christine Keeling ◽  
...  
Keyword(s):  
Plasma Glucose ◽  
Human Subjects ◽  
Dietary Vitamin ◽  
Vitamin B ◽  
Before And After

scholarly journals Measurement of renal tissue oxygenation with blood oxygen level-dependent MRI and oxygen transit modeling

2014 ◽  
Vol 306 (6) ◽  
pp. F579-F587 ◽  
Author(s):  
Jeff L. Zhang ◽  
Glen Morrell ◽  
Henry Rusinek ◽  
Lizette Warner ◽  
Pierre-Hugues Vivier ◽  
...  
Keyword(s):  
Tissue Oxygenation ◽  
Human Subjects ◽  
Blood Oxygen Level Dependent ◽  
Renal Tissue ◽  
Blood Oxygen ◽  
Oxygen Level ◽  
Blood Oxygen Level ◽  
Healthy Human ◽  
Bold Mri ◽  
Before And After

Blood oxygen level-dependent (BOLD) MRI data of kidney, while indicative of tissue oxygenation level (Po2), is in fact influenced by multiple confounding factors, such as R2, perfusion, oxygen permeability, and hematocrit. We aim to explore the feasibility of extracting tissue Po2 from renal BOLD data. A method of two steps was proposed: first, a Monte Carlo simulation to estimate blood oxygen saturation (SHb) from BOLD signals, and second, an oxygen transit model to convert SHb to tissue Po2. The proposed method was calibrated and validated with 20 pigs (12 before and after furosemide injection) in which BOLD-derived tissue Po2 was compared with microprobe-measured values. The method was then applied to nine healthy human subjects (age: 25.7 ± 3.0 yr) in whom BOLD was performed before and after furosemide. For the 12 pigs before furosemide injection, the proposed model estimated renal tissue Po2 with errors of 2.3 ± 5.2 mmHg (5.8 ± 13.4%) in cortex and −0.1 ± 4.5 mmHg (1.7 ± 18.1%) in medulla, compared with microprobe measurements. After injection of furosemide, the estimation errors were 6.9 ± 3.9 mmHg (14.2 ± 8.4%) for cortex and 2.6 ± 4.0 mmHg (7.7 ± 11.5%) for medulla. In the human subjects, BOLD-derived medullary Po2 increased from 16.0 ± 4.9 mmHg (SHb: 31 ± 11%) at baseline to 26.2 ± 3.1 mmHg (SHb: 53 ± 6%) at 5 min after furosemide injection, while cortical Po2 did not change significantly at ∼58 mmHg (SHb: 92 ± 1%). Our proposed method, validated with a porcine model, appears promising for estimating tissue Po2 from renal BOLD MRI data in human subjects.

scholarly journals Chronic Use of Opioid Medications Before and After Bariatric Surgery

JAMA ◽  
2013 ◽  
Vol 310 (13) ◽  
pp. 1369 ◽  
Author(s):  
Marsha A. Raebel ◽  
Sophia R. Newcomer ◽  
Liza M. Reifler ◽  
Denise Boudreau ◽  
Thomas E. Elliott ◽  
...  
Keyword(s):  
Bariatric Surgery ◽  
Before And After ◽  
Opioid Medications ◽  
Chronic Use

scholarly journals Circulating vaspin and visfatin are not affected by acute or chronic energy deficiency or leptin administration in humans

2011 ◽  
Vol 164 (6) ◽  
pp. 911-917 ◽  
Author(s):  
Eun Seok Kang ◽  
Faidon Magkos ◽  
Elizabeth Sienkiewicz ◽  
Christos S Mantzoros
Keyword(s):  
Human Subjects ◽  
Normal Weight ◽  
Hypothalamic Amenorrhea ◽  
Energy Deficiency ◽  
Chronic Energy Deficiency ◽  
Before And After ◽  
Term Energy ◽  
Energy Deprivation

ObjectiveAnimal and in vitro studies indicate that leptin alleviates starvation-induced reduction in circulating vaspin and stimulates the production of visfatin. We thus examined whether vaspin and visfatin are affected by short- and long-term energy deprivation and leptin administration in human subjects in vivo.Design and methodsWe measured circulating levels of vaspin and visfatin i) before and after 72 h of starvation (leading to severe hypoleptinemia) with or without leptin administration in replacement doses in 13 normal-weight subjects, ii) before and after 72 h of starvation with leptin administration in pharmacological doses in 13 lean and obese subjects, iii) during chronic energy deficiency in eight women with hypothalamic amenorrhea on leptin replacement for 3 months, and iv) during chronic energy deficiency in 18 women with hypothalamic amenorrhea on leptin replacement or placebo for 3 months.ResultsAcute starvation decreased serum leptin to 21% of baseline values, (P=0.002) but had no significant effect on vaspin and visfatin concentrations (P>0.05). Nor did normalization of leptin levels affect the concentrations of these two adipokines (P>0.9). Leptin replacement in women with hypothalamic amenorrhea did not significantly alter vaspin and visfatin concentrations, whether relative to baseline or placebo administration (P>0.25). Pharmacological doses of leptin did not affect circulating vaspin and visfatin concentrations (P>0.9).ConclusionsCirculating vaspin and visfatin are not affected by acute or chronic energy deficiency leading to hypoleptinemia and are not regulated by leptin in human subjects, indicating that these adipocyte-secreted hormonal regulators of metabolism are independently regulated in humans.

Glycine Propionyl-L-carnitine Modulates Lipid Peroxidation and Nitric Oxide in Human Subjects

2009 ◽  
Vol 79 (3) ◽  
pp. 131-141 ◽  
Author(s):  
Richard J. Bloomer ◽  
Lesley C. Tschume ◽  
Webb A. Smith
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Aerobic Exercise ◽  
Human Subjects ◽  
Blood Lipid ◽  
Lipid Profiles ◽  
Post Intervention ◽  
Double Blind ◽  
Before And After ◽  
Main Effects

Objective: To determine the efficacy of glycine propionyl-L-carnitine (GPLC) to decrease lipid peroxidation, elevate nitric oxide, and improve blood lipid profiles in human subjects. Methods: Thirty untrained, normolipidemic subjects performed eight weeks of supervised aerobic exercise while supplementing GPLC at one of two doses (1 or 3 grams daily of PLC + glycine) or placebo, following random assignment in a double-blind manner. Fasting blood samples were analyzed at rest for malondialdehyde, nitric oxide, and lipids before and after the intervention. Results: Malondialdehyde was decreased (p<0.05) from pre- to post-intervention with 1 g GPLC (1.08±0.24 vs. 0.69±0.25 µmol·L -1) and 3 g GPLC (0.94±0.18 vs. 0.66±0.17 µmol·L -1), but did not change statistically (p>0.05) with placebo (1.12±0.21 vs. 1.03±0.23 µmol·L -1). Nitric oxide was increased (p<0.05) from pre- to post-intervention with 3 g GPLC (21.34±2.27 vs. 29.46±3.61 µmol·L -1), but did not change statistically (p>0.05) with 1 g GPLC (23.22±4.13 vs. 26.24±4.32 µmol·L -1) or placebo (24.31±3.90 vs. 26.14±4.11 µmol·L -1). No main effects or interaction effects were noted for blood lipids (p>0.05). Conclusion: GPLC supplementation combined with eight weeks of aerobic exercise decreases lipid peroxidation and elevates nitric oxide, but does not further improve blood lipid profiles in normolipidemic subjects.

scholarly journals Association betweenFaecalibacterium prausnitziiand dietary fibre in colonic fermentation in healthy human subjects

2010 ◽  
Vol 104 (5) ◽  
pp. 693-700 ◽  
Author(s):  
Robin F. J. Benus ◽  
Tjip S. van der Werf ◽  
Gjalt W. Welling ◽  
Patricia A. Judd ◽  
Moira A. Taylor ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Dietary Fibre ◽  
Human Subjects ◽  
Healthy Human ◽  
Formulated Diets ◽  
Disease States ◽  
Before And After ◽  
Complex Ecosystem ◽  
Positive Correlations ◽  
Proportional Reduction

The intestinal microbiota are a complex ecosystem influencing the immunoregulation of the human host, providing protection from colonising pathogens and producing SCFA as the main energy source of colonocytes. Our objective was to investigate the effect of dietary fibre exclusion and supplementation on the intestinal microbiota and SCFA concentrations. Faecal samples were obtained from healthy volunteers before and after two 14 d periods of consuming formulated diets devoid or supplemented with fibre (14 g/l). The faecal microbiota were analysed using fluorescentin situhybridisation and SCFA were measured using GLC. There were large and statistically significant reductions in the numbers of theFaecalibacterium prausnitzii(P ≤ 0·01) andRoseburiaspp. (P ≤ 0·01) groups during both the fibre-free and fibre-supplemented diets. Significant and strong positive correlations between the proportion ofF. prausnitziiand the proportion of butyrate during both baseline normal diets were found (pre-fibre freer0·881,P = 0·001; pre-fibre supplementedr0·844,P = 0·002). A significant correlation was also found between the proportional reduction inF. prausnitziiand the proportional reduction in faecal butyrate during both the fibre-free (r0·806;P = 0·005) and the fibre-supplemented diet (r0·749;P = 0·013). These findings may contribute to the understanding of the association between fibre, microbiota and fermentation in health, during enteral nutrition and in disease states such as Crohn's disease.

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