Chemical Composition and Antimicrobial Activity of Bark and Leaf Extracts of Cupressus sempervirens and Juniperus phoenicea Grown in Al- Jabel Al-Akhdar Region, Libya

2019 ◽  
Vol 9 (4) ◽  
pp. 268-279
Author(s):  
Mohamed E.I. Badawy ◽  
Ibrahim E.A. Kherallah ◽  
Ahmed S.O. Mohareb ◽  
Mohamed. Z.M. Salem ◽  
Hameda A. Yousef
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Sea Level ◽  
Plant Extracts ◽  
Complex Mixture ◽  
Biologically Active ◽  
Cupressus Sempervirens ◽  
Leaf Extracts ◽  
Juniperus Phoenicea ◽  
The Impact

Background:Plant extracts are important products in the world and have been widely used for isolation of important biologically active products. Because of their significant environmental impact, extensive research has been explored to determine the antimicrobial activity of plant extracts.Methods:Acetone extracts of the bark and leaf of Cupressus sempervirens and Juniperus phoenicea, collected from three different altitudes (125, 391, and 851 m high of sea level) at Al- Jabel Al-Akhdar area, Libya were obtained and analyzed by GC/MS. The antimicrobial activity of the extracts was further evaluated against plant bacteria Rhizobium radiobacter, Erwinia carotovora, Rhodococcus fascians and Ralstonia solanacearum and fungus Botrytis cinerea.Results:The impact of the altitude from the sea level on the quantity and chemical constituents of the extracts was investigated. The yield was largely dependent on tree species and the highest yield (6.50%) was obtained with C. sempervirens L bark of altitude III (851 m of the sea level), while the lowest (1.17%) was obtained with the leaf extract of C. sempervirens L from altitude I (125 m). The chemical composition analyzed by GC/MS confirmed that the leaf extracts of C. sempervirens and J. phoenicea contained a complex mixture of monoterpene hydrocarbons, sesquiterpenes, diterpenes, diterpenoids, terpenophenolic, steroids and phthalates. However, the bark extracts of both trees contained a mixture of sesquiterpenes, diterpenes, diterpenoids, terpenophenolics, phthalates, retinol and steroids. These constituents revealed some variability among the extracts displaying the highest interesting chemotype of totarol (terpenophenolic) in all extracts (14.63-78.19% of the total extract). The extracts displayed a noteworthy antifungal potency with varying degrees of inhibition of growth with EC50 values ranged from 78.50 to 206.90 mg/L. The extracts obtained from the leaves of C. sempervirens showed that the highest inhibitory activity was obtained with the extract of altitude II (391 m) with MIC 565, 510, 380 and 710 mg/L against E. carotovora, R. fascians, and R. radiobacter and R. solanacearum, respectively.Conclusion:Based on antimicrobial activity, raw plant extracts can be a cost-effective way to protect crops from microbial pathogens. Because plant extracts contain several antimicrobial compounds, the development of resistant pathogens can be delayed.

scholarly journals Antimicrobial activity of leaf extracts of Euphorbia paralias L. and Melilotus sulcatus Desf. against some pathogenic microorganisms

2021 ◽  
pp. 10-15
Author(s):  
Miloud M. Miloud ◽  
Najma A. Senussi
Keyword(s):  
Antimicrobial Activity ◽  
Plant Extracts ◽  
Methanolic Extract ◽  
Bacterial Species ◽  
Fungal Species ◽  
Leaf Extracts ◽  
Methanolic Extracts ◽  
Bioactive Agents ◽  
Agar Well Diffusion Assay ◽  
Diffusion Assay

The present study was aimed to investigate the antimicrobial potential of leaf extracts of Euphorbia paralias and Melilotus sulcatus against four bacterial species Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli and Klebsiella sp. and two fungal species Asperigillus niger and Aspergillus flavus. The agar well diffusion assay was used to evaluate the antimicrobial activity. The effect of these extracts was most effective against the bacterial species compared to the fungal species at a used concentration (100 mg/ml). Methanolic extracts of selected plants displayed good antimicrobial activity against all tested microorganisms species, while, no activity for aqueous extracts against tested fungal species. Methanolic extracts were the most effective plant extracts against all tested bacterial species, with MIC and MBC reached 6.2 and 12.5 mg/ml, except Klebsiella sp. which was less sensitive to M. sulcatus methanolic extract and its MIC and MBC reached 12.5 and 25 mg/ml, respectively. These plant extracts which proved to be potentially effective can be used as bioactive agents to control microorganisms caused for diseases and they can be used naturally in the human and veterinary healthcare systems.

scholarly journals The impact of the agrochemical properties of soil on the chemical composition of Jerusalem artichoke flowers

2019 ◽  
Vol 26 (3) ◽  
Author(s):  
Ieva Stočkutė ◽  
Elvyra Jarienė
Keyword(s):  
Chemical Composition ◽  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Complex Mixture ◽  
Jerusalem Artichoke ◽  
Quality Analysis ◽  
Volatile Organic ◽  
Ray Florets ◽  
The Impact ◽  
Different Parts

The objective of this research was to investigate and to evaluate the chemical composition of Jerusalem artichoke flowers grown in different soils. Quality analysis was carried out in the laboratories of the Institute of Agricultural and Food Science of Vytautas Magnus University. The following agrochemical parameters of the soil were determined using standardized methods: pHKCl, the amount of soil humus, mineral nitrogen, mobile phosphorus (P) and potassium (K). The chemical composition of different parts of Jerusalem artichoke flowers (disk florets and ray florets) were evaluated by the standard method: the content of carbohydrates (inulin and total saccharides content), minerals (N, P, K, Ca, Fe, Na, Zn, Mg). Electronic nose (Alpha M. O. S.) measurement technologies were used to recognize and identify the flower fragrance (volatile organic compounds). Processing of the research data was carried out through the application of the analysis of variance (ANOVA), using the computer software Statistica 10. The research results showed that the substantially highest amount of inulin was determined in the disk florets (0.339%, D. M.) of Jerusalem artichoke grown in the soil with the medium humus and medium available potassium amount. The content of total soluble saccharides of different parts of Jerusalem artichoke flowers varied from 2.54 to 4.11% of disk florets and from 0.55 to 0.81% of ray florets. The highest amount of macroelement potassium (3.1%, D. M.) was detected in Jerusalem artichoke flowers grown in the soil with the medium amount of humus and the medium amount of mobile potassium. Volatile organic compounds in Jerusalem artichoke flowers were determined as a complex mixture of esters, alcohols, terpenes, sulphur and other compounds. Esters as volatile compounds prevailed in Jerusalem artichoke flowers. Methyl acetate and ethyl 2-methylbutyrate with fruity, grape odour were the predominant esters group compounds as well as dimethyl trisulfide – sulfur compound in Jerusalem artichoke flowers. The investigated aroma profile of flowers shows that they have fruity-like odour.

Chemical Composition and Antimicrobial Activity of Essential oil from Scales of Moroccan Juniperus phoenicea

2021 ◽  
Vol 1 (30) ◽  
pp. 5-11
Author(s):  
M. Achir M. Dakir
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antifungal Agent ◽  
Antibacterial Agent ◽  
Oil Composition ◽  
High Biological Activity ◽  
Juniperus Phoenicea ◽  
Total Oil ◽  
Antibacterial And Antifungal

Abstract-The essential oil of Juniperus phoenicea was obtained by hydrodistillation method using a Clevenger-type apparatus with a yield of 1.9 % and was analyzed by gas chromatography coupled to a mass spectrometer (GC-MS). Twelve volatile compounds were identified representing 99,85% of the total oil composition, while the α-pinene (78,31%), β-Myrcene (11,92%) and limonene (3,96%) were the major compounds. This essential oil was evaluated as an antibacterial and antifungal agent. The result showed that the oil presents a high biological activity as an antibacterial agent against the three tested strains Escherichia coli, Staphylococcus aureus and Pseudomonas aeroginos. It's also active as an antifungal agent against the Candida albicans with a zone inhibition of 28 mm. Keywords: Medicinal plant, Juniperus, phoenicea, Essential oil, Chemical composition, Antimicrobial activity.

scholarly journals Chemical Investigation of a Biologically Active Schinus molle L. Leaf Extract

2019 ◽  
Vol 2019 ◽  
pp. 1-6 ◽  
Author(s):  
Stefania Garzoli ◽  
Valentina Laghezza Masci ◽  
Elisa Ovidi ◽  
Giovanni Turchetti ◽  
Daniele Zago ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Cytotoxic Activity ◽  
Biological Activities ◽  
Petroleum Ether Extract ◽  
Folk Medicine ◽  
Biological Properties ◽  
Biologically Active ◽  
Leaf Extracts ◽  
Leukaemia Cell Line ◽  
Schinus Molle

The pepper tree Schinus molle L. is an evergreen ornamental plant belonging to the Anacardiaceae family, native to South America and widespread throughout the world. It has biological activities and is used in folk medicine. This paper aims to contribute to a deeper knowledge of its chemical composition and biological properties. S. molle leaf extracts were obtained by sequential extraction with solvents of different polarities and subsequently tested on the HL-60 human leukaemia cell line to define a possible cytotoxic activity. Among the investigated extracts, the petroleum ether extract revealed a high cytotoxic activity, and its chemical composition was further investigated. By a silica column chromatography, eight fractions were obtained, and their compositions were determined by GC-MS analysis. Compounds and relative abundance differed widely among the fractions; sesquiterpenes resulted the main component and alcoholic sesquiterpenes the most abundant.

Chemical composition and antimicrobial activity of Osage orange ( Maclura pomifera ) leaf extracts

2020 ◽  
Author(s):  
Snežana Filip ◽  
Saša Đurović ◽  
Stevan Blagojević ◽  
Ana Tomić ◽  
Aleksandra Ranitović ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Leaf Extracts ◽  
Maclura Pomifera ◽  
Osage Orange

Evaluation of the chemical composition and antimicrobial activity of Mentha pulegium, Juniperus phoenicea, and Cyperus longus essential oils from Morocco

2012 ◽  
Vol 45 (1) ◽  
pp. 313-319 ◽  
Author(s):  
Abdenour Ait-Ouazzou ◽  
Susana Lorán ◽  
Abdelhay Arakrak ◽  
Amin Laglaoui ◽  
Carmen Rota ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Mentha Pulegium ◽  
Juniperus Phoenicea

scholarly journals PHENOLIC COMPOUNDS OF CEDAR WOOD (SIBERIAN PINE) PINUS SIBIRICA DU TOUR.

2020 ◽  
pp. 97-104
Author(s):  
Tat'yana Yevgen'yevna Fedorova ◽  
Sergey Vladimirovich Fedorov ◽  
Vasily Anatolyevich Babkin
Keyword(s):  
Chemical Composition ◽  
Phenolic Compounds ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Complex Mixture ◽  
13C Nmr Spectroscopy ◽  
Biologically Active ◽  
Pinus Sibirica ◽  
Pinus Sibirica Du Tour ◽  
Siberian Pine

As part of the study of extractives of biomass of coniferous trees of Siberia in order to predict the possibility of obtaining valuable biologically active products, a study was conducted of the chemical composition of the ethyl acetate extract of cedar wood (Siberian pine) Pinus sibirica Du Tour. The chemical composition of the phenolic compounds of cedar wood was studied, including the structure of oligomeric polyphenols. The yields of extractives extracted with ethyl acetate and water from cedar wood were 1.6 and 2.71% of a.s. (absolutely dry matter). The content of phenolic compounds in the ethyl acetate extract of cedar wood was 45.9% rel. Based on 1H and 13C NMR spectroscopy data, it was found that the main monomeric phenolic extractive substances of cedar wood are stilbene – pinosilvin monomethyl ether (content in cedar wood 0.25% of a.s.) and flavonoids – tectochrizin, pinocembrin, and pinobankxin, as well as oligomeric and polymeric phenolic compounds. It was shown that the composition of cedar wood oligomers includes a complex mixture of stilbene derivatives of pinosilvin and flavonoids with the inclusion of carbohydrate residues. The content of the fraction of oligomeric and polymeric phenolic compounds in cedar wood is 0.39% of a.s.

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