Cytotoxic Constituents from the Bark of Erythrina poeppigiana Against the MCF-7 Breast Cancer Cell Lines

2020 ◽  
Vol 10 (3) ◽  
pp. 257-261
Author(s):  
Tati Herlina ◽  
Merlin ◽  
Mohd. Azlan ◽  
Unang Supratman
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Chemical Structure ◽  
Breast Cancer Cell Lines ◽  
Erythrina Poeppigiana ◽  
Structure Activity ◽  
Cancer Line ◽  
Cytotoxic Compounds ◽  
Ic50 Values ◽  
Mcf 7

Background: Erythrina poeppigiana (Leguminosae) is a high-growing plant with an orange flower that is widely distributed in tropical and subtropical countries. This particular plant is widely used in traditional medicine for gynecological complications and the treatment of various diseases. There exists no previous information regarding cytotoxic compounds from this plant. Objective: This research is to isolate cytotoxic compounds from E. poeppigiana. Methods: The isolation step was carried out using a combination of chromatographic techniques to obtain isolated three compounds (1, 2, and 3). Results: The chemical structure of isolated compounds was elucidated by spectroscopic methods and identified as β-erythroidine (1), 8-oxo-β-erythroidine (2), and 8-oxo-α-erythroidine (3). Compounds (1-3) showed cytotoxic activity against MCF-7 breast cancer line with IC50 values of 36.8, 60.8 and 875.4 μM, respectively. Conclusion: Three compounds have been successfully isolated from Erythrina poeppigiana (Leguminosae), showing cytotoxic properties against MCF-7 breast cancer line. Structure-activity relationship studies showed that the presence of enone moiety on compound 1 can reduce its cytotoxic activity towards MCF-7 breast cancer line.

Cytotoxic activity and anti-cancer potential of Ontario grown onion extracts against breast cancer cell lines

2018 ◽  
Vol 8 (3) ◽  
pp. 159 ◽  
Author(s):  
Meghan Fragis ◽  
Abdulmonem I. Murayyan ◽  
Suresh Neethirajan
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
Cytotoxic Activities ◽  
Cancer Line ◽  
Mcf 7

Background: Breast cancer is the most commonly diagnosed cancer and the second leading cause of cancer deaths among Canadian women. Cancer management through changes in lifestyle, such as increased intake of foods rich in dietary flavonoids, have been shown to decrease the risk associated with breast, liver, colorectal, and upper-digestive cancers in epidemiologic studies. Onions are high in flavonoid content and one of the most common vegetables. Additionally, onions are used in most Canadian cuisines.Methods: We investigated the effect of five prominent Ontario grown onion (Stanley, Ruby Ring, LaSalle, Fortress, and Safrane) extracts on two subtypes of breast cancer cell lines: a triple negative breast cancer line MDA-MB-231 and an ER+ breast cancer line MCF-7.Results: These onion extracts elicited strong anti-proliferative, anti-migratory, and cytotoxic activities on both the cancer cell lines. Flavonoids present in these onion extracts induced apoptosis, cell cycle arrest in the G2/M phase, and a reduction in mitochondrial membrane potential at dose-dependent concentrations. Onion extracts were more effective against MDA-MB-231 compared to the MCF-7 cell line. Conclusion: In this study, we investigated the extracts synthesized from Ontario-grown onion varieties in inducing anti-migratory, cytostatic, and cytotoxic activities in two sub-types of human breast cancer cell lines. Anti-tumor activity of these extracts depends upon the varietal and can be formulated into nutraceuticals and functional foods for the wellbeing of cancer patients. Overall, the results suggest that onion extracts are a good source of flavonoids with anti-cancerous properties.Keywords: onion extracts; flavonoids; anti-proliferative; breast cancer; cytotoxic activity

scholarly journals Senyawa Damara-20,24-dien-3-on yang beraktivitas Sitotoksis terhadap Sel Mcf-7 dari Kulit Batang Chisocheton Marcrophyllus (Meliaceae)

2015 ◽  
Vol 5 (3) ◽  
Author(s):  
Nurlelasari Nurlelasari ◽  
Nurabi Ferdiana ◽  
Subekti Mauluddin ◽  
Desi Harneti ◽  
Moelyono Moektiwardoyo ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Breast Cancer Cell ◽  
Human Breast Cancer ◽  
Chemical Structure ◽  
Human Breast Cancer Cell ◽  
Breast Cancer Cell Lines ◽  
Human Breast ◽  
Ic50 Value ◽  
Mcf 7

AbstrakSenyawa triterpenoid damaran, damara-20,24-dien-3-on telah diisolasi dari kulit batang Chisocheton Marcrophyllus (Meliaceae). Struktur kimia kedua senyawa tersebut telah ditetapkan berdasarkan data spektroskopi dan perbandingan data spektra yang diperoleh dari literatur. Senyawa damara-20,24-dien-3-on menunjukkan aktivitas sitotoksik terhadap sel kanker payudara manusia MCF-7 dengan nilai IC50 28 mM.Kata Kunci : aktivitas sitotoksik, sel MCF-7, triterpenoid damaran, Chisocheton Marcrophyllus,  MeliaceaeAbstractDammarane triterpenoid, damara-20,24-dien-3-on has been isolated from the bark of Chisocheton Marcrophyllus (Meliaceae). Chemical Structure of compound was dtermined on the basic of spektroscopic data and comparison with those spectra data previously reported. Compound damara-20,24-dien-3-on exhibited cytotoxic activity againts MCF-7 human breast cancer cell lines with IC50 value 28 mM respectively.Kata Kunci : cytotoxic activity, MCF-7 cell, dammarane triterpenoid, Chisocheton Marcrophyllus,  Meliaceae

scholarly journals A Nitrocarbazole as a New Microtubule-Targeting Agent in Breast Cancer Treatment

2021 ◽  
Vol 11 (19) ◽  
pp. 9139
Author(s):  
Maria Stefania Sinicropi ◽  
Cinzia Tavani ◽  
Camillo Rosano ◽  
Jessica Ceramella ◽  
Domenico Iacopetta ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Anticancer Activity ◽  
Breast Cancer Cell Lines ◽  
Cellular Functions ◽  
Anticancer Properties ◽  
Cycle Arrest ◽  
In Silico Studies ◽  
Ic50 Values ◽  
Mcf 7

Breast cancer is still considered a high-incidence disease, and numerous are the research efforts for the development of new useful and effective therapies. Among anticancer drugs, carbazole compounds are largely studied for their anticancer properties and their ability to interfere with specific targets, such as microtubule components. The latter are involved in vital cellular functions, and the perturbation of their dynamics leads to cell cycle arrest and subsequent apoptosis. In this context, we report the anticancer activity of a series of carbazole analogues 1–8. Among them, 2-nitrocarbazole 1 exhibited the best cytotoxic profile, showing good anticancer activity against two breast cancer cell lines, namely MCF-7 and MDA-MB-231, with IC50 values of 7 ± 1.0 and 11.6 ± 0.8 μM, respectively. Furthermore, compound 1 did not interfere with the growth of the normal cell line MCF-10A, contrarily to Ellipticine, a well-known carbazole derivative used as a reference molecule. Finally, in vitro immunofluorescence analysis and in silico studies allowed us to demonstrate the ability of compound 1 to interfere with tubulin organization, similarly to vinblastine: a feature that results in triggering MCF-7 cell death by apoptosis, as demonstrated using a TUNEL assay.

scholarly journals LC-MS/MS and Cytotoxic Activity Analysis of Extract and Fraction of Calophyllum soulattri Stembark

2021 ◽  
pp. 356-364
Author(s):  
Muhamad Salman Fareza ◽  
Nur Amalia Choironi ◽  
Sri Sutji Susilowati ◽  
Melina Puspita Rini ◽  
Vyola Festihawa ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Stem Bark ◽  
Positive Control ◽  
Bark Extract ◽  
Ic50 Values ◽  
Stem Bark Extract ◽  
Mcf 7

Calophyllum soulattri (Sulatri), a plant from Clusiaceae family, has been empirically used as traditional medicine. In the present study, C. soulattri stem bark extract and fractions were evaluated for their toxicity against MCF-7 breast cancer cell. The extract and fraction’s chemical content was analyzed using the combination of liquid chromatography with mass spectrometry (LC-MS/MS). The results showed that methanol extract and n-hexane fractions have strong cytotoxic activity with IC50 values 93.6 and 36 µg/mL, respectively. Meanwhile, as the positive control, ethyl acetate and cisplatin fractions have IC50 values 233 and 16.2 µg/mL, respectively. The LC-MS/MS analysis showed that the extract and fractions contained polyporusterone A, poricoic acid D, polyporusterone F, esulentagenin, and 1-acetyl-3-(methoxy-carbonyl)-β-carboline. Therefore, C. soulattri stem bark extract and fractions have potential activity as an anticancer agent that was able to inhibit MCF-7 breast cancer cell growth.  

Catechin Loaded Poly(lactic-co-glycolic acid) Nanoparticles: Characterization, Antioxidant and Cytotoxic Activity Against MCF-7 Breast Cancer Cells

2020 ◽  
Vol 20 (9) ◽  
pp. 5313-5321
Author(s):  
Burcu Turkoglu ◽  
Banu Mansuroglu
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Encapsulation Efficiency ◽  
Average Particle Size ◽  
Antioxidant Properties ◽  
Breast Cancer Cell Lines ◽  
Reaction Yield ◽  
Average Particle ◽  
Dpph Method ◽  
Mcf 7

Here we evaluate the cytotoxic and antioxidant properties of Catechin (Cat) loaded nanoparticles (CatNps) on breast cancer cell lines. CatNps were prepared by the modified single emulsion solvent evaporation method. The structural, physical and chemical properties of the CatNps including PLGA-Cat interactions, and surface characteristics, were analyzed by dynamic light scattering (DLS), Fourier transform infrared (FT-IR), and Scanning Electron Microscopy (SEM). The average particle size of CatNps was 190.5±1.762 nm with an encapsulation efficiency of 10% and zeta potential value of 13.7±1.258 mV. The CatNps had reaction yield of 43.47±1.1% and loading capacity of 3.710±2.6% Treating MCF-7 cells with CatNps for 48 h led to a decrease in cell viability with an of IC50 22.59 μg/mL. The antioxidant behavior of CatNps was evaluated by the DPPH method in various pH environments (pH 3-, 6-, 7.4-, 9- and 12). Despite the low encapsulation efficiency, CatNps showed significant antioxidant efficacy against DPPH radicals relative to Cat. Our results revealed that CatNps could be used as promising anti-cancer agents due to their enhanced cytotoxic activity, increased bioavailability, and antioxidant properties.

scholarly journals Indonesian Micromelum minutum Leave Extracts and Their Cytotoxic Activities toward Breast Cancer Cell Lines

2021 ◽  
Vol 53 (1) ◽  
pp. 109-117
Author(s):  
Ratna Asmah Susidarti ◽  
Edy Meiyanto ◽  
Muthi' Ikawati ◽  
Normaidah Normaidah ◽  
Nurramadhani A. Sida
Keyword(s):  
Breast Cancer ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cell Lines ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cancer Cell Lines ◽  
Breast Cancer Cell Lines ◽  
4T1 Cells ◽  
Mcf 7

Isolation and identification of compounds and pharmacological activity of the Micromelum minutum grown in some countries has been done, but the Indonesian M. minutum has not been studied, either phytochemically or pharmacologically. This study aimed to determine the cytotoxic activity of Indonesian M. minutum leave extracts toward MCF-7 and 4T1 breast cancer cell lines. The leaves were obtained from M. minutum grow in Bantimurung National Park, Bulusaraung, South Sulawesi, and then were macerated gradually in hexane, ethyl acetate, and methanol. The cytotoxic activity of obtained extracts was determined by MTT assay. The extraction yielded hexane (HEM), ethyl acetate (EEM), and methanol (MEM) extracts of 2.65, 6.12, and 6.49%, respectively. HEM was the most potent extract with IC50 values of 148 and 87 µg/mL on MCF-7 and 4T1 cells, respectively, followed by EEM (185 and 170 µg/mL). MEM possessed a weak potency with an IC50 value of 384 µg/mL on MCF-7 cells and was not toxic toward 4T1 cells. Therefore, HEM is important to be further investigated for its active constituents.

scholarly journals Biotinylated xanthohumol: synthesis and in vitro biological evaluation for anticancer therapy

2018 ◽  
Author(s):  
Monika Stompor ◽  
Rafał Podgórski ◽  
Marta Świtalska ◽  
Joanna Wietrzyk
Keyword(s):  
Breast Cancer ◽  
Antioxidant Activity ◽  
Cancer Cells ◽  
Cell Lines ◽  
Biological Evaluation ◽  
Breast Cancer Cell Lines ◽  
Dpph Method ◽  
Ic50 Values ◽  
Mcf 7

Two biotinylated derivatives of the main hop chalcone xanthohumol (1) were prepared by a one-step synthesis via esterification using biotin and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC HCl) and 4-dimethylaminopyridine (DMAP) as coupling reagents. The products were characterized spectroscopically and their antiproliferative activity toward MCF-7, MCF-10A, HepG2, MDA-MB-231, 4T1 and Balb/3T3 cell lines was investigated using the SRB assay. For all three tested compounds the best activity was noted in the case of human (MCF-7) and mice (4T1) breast cancer cell lines (IC50 values < 9 μM). Both biotinylated derivatives showed higher anticancer activity than xanthohumol (1) towards all types of tested breast cancer cells. Double biotinylated xanthohumol (3) proved to be the most active in inhibiting cell growth, with IC50 values equal to 5.35 ± 1.5 μM for 4T1 and 8.03 ± 0.53 µM for MCF-7 cell lines. Compound 3 was also more active than 1 and 2 against liver cancer cells HepG2 (IC50 = 17.37 ± 5.1 μM), while the IC50 values for 1 and 2 were equal to 21.5 ± 2.7 and 22.1 ± 3.9 µM, respectively. 4‑O‑biotinylxanthohumol (2) was the second most active growth inhibitor, particularly with respect to MCF-7 (IC50 = 6.19 ± 1.7 μM) and 4T1 (IC50 = 6.64 ± 0.4 μM) cell lines. The antioxidant activity was evaluated using the 1.1-diphenyl-2-picrylhydrazyl radical (DPPH) method. All tested compounds (1-3) have antioxidant activity between 2.73 and 3.38 mM. It was reported for the first time that new prenylated chalcones containing the biotin moiety effectively inhibited proliferation of cancer cells in vitro.

scholarly journals Triterpenoids from The Bark of Garcinia porecta and their Cytotoxic Activity against MCF7 Breast Cancer Lines

2019 ◽  
Vol 15 (1) ◽  
pp. 1
Author(s):  
Darwati Darwati ◽  
Alya Tsamrotul ◽  
Tati Herlina ◽  
Tri Mayanti ◽  
Nurlelasari Nurlelasari ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cytotoxic Activity ◽  
Structure Elucidation ◽  
Breast Cancer Cells ◽  
Chemical Structure ◽  
Spectrometric Analysis ◽  
Tropical Plant ◽  
Phytochemical Studies ◽  
First Time ◽  
Mcf 7

The <em>Garcinia</em> genus is a well known tropical plant in the Indo-Malesiana region and mainly distributed in tropical countries including Indonesia, Thailand, and Malaysia. Previous phytochemical studies on <em>Garcinia </em>species have led to the identification and isolation of mainly prenylated xanthones. This research describes the isolation and structure elucidation of isolated triterpenoids compounds from the bark of <em>Garcinia porecta</em>. Dried powder bark of <em>G. porecta </em>was extracted with methanol and then partitioned with <em>n</em>-hexane, ethyl acetate, and <em>n</em>-butanol. The <em>n</em>-hexane extract then was separated and purified with chromatography techniques to obtain isolated compounds <strong>1</strong><strong> </strong>and<strong> 2</strong>. The chemical structure of isolated compounds was elucidated by spectroscopic methods including one and two-dimensional NMR as well as high-resolution mass spectrometric analysis and identified as lanosterol (<strong>1</strong>) dan arabidiol (<strong>2</strong>), respectively. These triterpenoids were isolated from this plant for the first time. Compound <strong>1</strong> and <strong>2</strong> showed weak cytotoxic activity against MCF-7 breast cancer cells with IC<sub>50</sub> values of 60.09 dan 46.17 µM, respectively.

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