Antidiabetic Activity of the Methanolic Extracts of Thuja occidentalis Twings in Alloxan-induced Rats

Objective and Background: In hyperglycemic and alloxan-induced diabetic rats, the effect of Thuja occidentalis methanolic residue extract on blood glucose levels and some biochemical parameters were investigated. Methods: Significantly decreased blood glucose level by 22.85% and 27.66%, in hyperglycemic rats, respectively after 3 h, were seen in a single oral administration of the extract at a dose of 200 and 400 mg/kg. Blood glucose level was decreased by 50% in alloxan-induced diabetic rats within three weeks of daily treatment of Thuja occidentalis methanolic residue extract (200 and 400 mg/kg p.o). Alloxan-induced diabetic rats showed significant hypercholesterolemia in comparison with the control in alloxan induced rats. Results: In both normal and diabetic rats, there was also a significant decrease of elevated serum cholesterol and triglycerides. Hypertriglyceridemia was also shown to be prevented by treatment with plant extract (200 and 400mg/kg p.o) (p<0. 05). As compared with the control animals, diabetic control rats did not show any change in a level of creatinine and urea. Conclusion: Thuja occidentalis treatment may improve glucose homeostasis in alloxaninduced diabetes and alleviate kidney and liver function. Thuja occidentalis twigs could be a potential source of the new oral antidiabetic drug.

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Antidiabetic Activity ofAcacia tortilis(Forsk.) Hayne ssp. raddiana Polysaccharide on Streptozotocin-Nicotinamide Induced Diabetic Rats

BioMed Research International ◽

10.1155/2014/572013 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 9

Author(s):

Pradeep Kumar Bhateja ◽

Randhir Singh

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Total Cholesterol ◽

Glucose Level ◽

Fasting Blood Glucose ◽

Diabetic Control ◽

Diabetic Rats ◽

Antidiabetic Activity ◽

Control Group ◽

Fasting Blood Glucose Level

The present study was designed to investigate the antidiabetic activity of aqueous extract ofAcacia tortilispolysaccharide (AEATP) from gum exudates and its role in comorbidities associated with diabetes in STZ-nicotinamide induced diabetic rats. Male albino Wistar rats were divided into control, diabetic control, glimepiride treated (10 mg/kg), and diabetic rats treated with 250, 500, and 1000 mg/kg dose of AEATP groups and fasting blood glucose, glycated hemoglobin, total cholesterol, triglyceride, LDL, VLDL, HDL, SGOT, and SGPT levels were measured. STZ significantly increased fasting blood glucose level, glycated hemoglobin, total cholesterol, triglyceride, LDL, VLDL, SGOT, and SGPT levels, whereas HDL level was reduced as compared to control group. After 7 days of administration, 500 and 1000 mg/kg dose of AEATP showed significant reduction(P<0.05)in fasting blood glucose level compared to diabetic control. AEATP has also reduced total cholesterol, triglyceride, LDL, VLDL, SGOT, and SGPT levels and improved HDL level as compared to diabetic control group. Our study is the first to report the normalization of fasting blood glucose level, lipid profile, and liver enzyme in AEATP treated diabetic rats. Thus, it can be concluded that AEATP may have potentials for the treatment of T2DM and its comorbidities.

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Pharmacodynamics Drug Interactions of Metformin with Aspirin and Nifedipine

Asian Journal of Pharmaceutical Research and Health Care ◽

10.18311/ajprhc/2016/683 ◽

2016 ◽

Vol 8 (1) ◽

pp. 4 ◽

Cited By ~ 1

Author(s):

Khidir A. M. Hassan ◽

Mahmoud M. E. Mudawi ◽

Mansour I. Sulaiman

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Diabetic Control ◽

Serum Glucose ◽

Diabetic Rats ◽

Control Group ◽

Citrate Buffer ◽

Treatment Groups ◽

Diabetic Control Group

Metformin is now being recognized as the standard therapy in T2D patients who are overweight. Metformin has many drug-disease interactions that can increase the risk of metformin-associated lactic acidosis. Therefore this study was conducted to evaluate any possible pharmacodynamic interactions between metformin and drugs used to treat chronic diseases e.g. Hypertension. The rats were fasted overnight before inducing diabetes with streptozotocin. The rats were given an intraperitoneal injection of streptozotocin (50 mg kg−1) freshly prepared in 0.1M sodium citrate buffer. The diabetic state was confirmed 72 h after streptozotocin injection. Diabetic rats were grouped into seven groups each group of five rats and distributed among the normal control group diabetic control group and the treatment groups. The treatment continued for 10 days. Blood samples were taken before treatment and after 10 days and analyzed for serum glucose, cholesterol, HDL, LDL, and triglycerides. In the diabetic control group which was given STZ alone the blood glucose level decreased significantly (p < 0.05) after 10 days but still above the hyperglycemic level (200mg/dl). The same was observed in the group treated with metformin. The group treated with nifedipine and aspirin showed significant reduction (p < 0.01) in the glucose level below the hyperglycemic level (200mg/dl). While the groups treated with (Metformin + Nifedipine) and (Metformin +Aspirin) showed highly significant reduction (P<0.001) in blood glucose level. These results conclude that the combination of (metformin +Nifedipine) and the combination of (Metformin + Aspirin) have highly significant hypoglycemic effect. It also showed that Nifedipine has promising role in reducing blood glucose level, lipid profile especially LDL-cholesterol, and body weight.

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Antidiabetic activity of musa acuminata colla fruit peel (MACFP) ethanol extract in glucose-induced diabetic rats

MATEC Web of Conferences ◽

10.1051/matecconf/201819707007 ◽

2018 ◽

Vol 197 ◽

pp. 07007

Author(s):

Erza Genatrika ◽

Visda Nila Happy Laksari ◽

Tjiptasurasa Tjiptasurasa

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Ethanol Extract ◽

Positive Control ◽

Diabetic Rats ◽

Musa Acuminata ◽

Antidiabetic Activity ◽

Control Group ◽

Fruit Peel

Diabetes mellitus is a metabolic disorder disease characterized by increased blood glucose levels due to impaired insulin secretion or increased insulin resistance. The study was performed to investigate the blood glucose lowering effect of Musa acuminata Colla fruit peel (MACFP) ethanol extract in experimentally induced diabetic rats. 25 rats were divided into five groups, i.e negative control group (0.5% NaCMC), positive control group (glibenclamide), and treatment group of MACFP ethanol extract with dose 250, 375, and 500 mg/kg body weight (mg/kg BW). The blood sample was taken from the lateral vein of the tail, and then blood glucose level was observed on 0, 30, 60, 90, and 120 minutes. The results provide information that MACFP ethanol extract with dose 250, 375, and 500 mg/kg BW have the same effect with a positive control (glibenclamide) in lowering blood glucose level on diabetic rats (p>0,05). MACFP ethanol extract with dose 500 mg/kg BW had the highest percentage of decrease in blood glucose level (42,62%), followed by doses 375 mg/kg BW (37,26%) and 250 mg/kg BW (24,12%).

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Antimicrobial and antidiabetic potentials of processed finger millet (Eleusine coracana)

International Journal of Biological Research ◽

10.14419/ijbr.v6i1.10726 ◽

2018 ◽

Vol 6 (1) ◽

pp. 18

Author(s):

Japhet Erasmus Aisoni ◽

Muhammad Yusha’u ◽

Olukayode Olugbenga Orole

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Finger Millet ◽

Islet Cells ◽

Salmonella Typhi ◽

Glycaemic Index ◽

Diabetic Rats ◽

Albino Rats ◽

Methanolic Extracts

Finger millet is a food crop with energy value, phytochemical constituents, and minerals, hence its use as whole flour for traditional food preparation among traditional people. The study was designed to determine the glycaemic index and blood glucose level in albino rats, and the antimicrobial potential of processed finger millets. Finger millets were processed by fermentation and roasting, and methanolic extracts obtained used to test antimicrobial activity. Albino rats (18) divided into three groups were fed fermented, roasted, and unprocessed millet (control), and glycaemic index and blood glucose level determined. Another 25 rat were treated into 5 groups of diabetic rats without treatment, diabetic rats treated daily with Metformin, diabetic rats fed 20% millet, diabetic rats fed 40% millet, and untreated group (control). At 100% concentration, roasted and fermented millet obtained inhibition of 15mm and 14mm against Salmonella typhi, while the control drug 25mg/ml streptomycin had 17mm inhibition. While values obtained for the glycemic indexes were close though significantly different from each other (roasted - 36.83±1.23; fermented - 38.73±1.87), roasted millet brought about the highest reduction in blood glucose level 90.9 and 65.1mg/dl at 60 and 120min respectively compared with fermented millet with 125.1 and 100.2mg/dl respectively at the same time. Animals administered finger millet in Groups 3 and 4-showed regeneration of the islet cells. The millet had the capacity to bring about healing of necrotic cells while also restoring and maintaining glucose levels in blood of subjects.

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EVALUATION OF PHYTOCHEMICAL PROFILE AND ANTIDIABETIC ACTIVITY OF FICUS RACEMOSA (LINN.) STEM BARK IN RATS

INDIAN DRUGS ◽

10.53879/id.54.01.10279 ◽

2017 ◽

Vol 54 (01) ◽

pp. 49-54

Author(s):

N. D Solanki ◽

S. K. Bhavsar ◽

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Diabetic Rats ◽

Antidiabetic Activity ◽

Phytochemical Analysis ◽

Phytochemical Profile ◽

Ficus Racemosa ◽

Tlc Analysis ◽

Mean Blood Glucose

Herbal plants are true bio-resources in traditional systems of medicine. Ficus racemosa (FR) Linn is a herb mentioned in ancient scriptures of Ayurveda having great medicinal importance. The present study aims to investigate phytochemical & antidiabetic activity of FR. Aqueous (AE) and ethanolic (EE) extracts of powdered shade dried bark of FR was used in the study. Phytochemical profile of extracts were evaluated by standard chemical tests & TLC analysis. The antidiabetic activity of AE and EE of FR was evaluated through STZ induced diabetes model by analyzing the blood glucose level before and after the study & mean blood glucose level through an HPLC method in Wistar rats. Prelimianry phytochemical analysis of EE showed presence of major phytoconstituents like alkaloid, glycoside, sterols, flavanoid, phenol, tannins while AE contains carbohydrates, cardiac glycosides, phytosterol, saponin, phenols, and protein. TLC analysis showed higher Rf value in EE in a different set of mobile phase. In acute toxicity study, a single oral dose (2000 mg/kg) did not produce any mortality in rats. 28 days treatment with AE (500 mg/kg) & EE (400 mg/kg) of FR showed a significant reduction (P<0.05) in blood glucose level compared to diabetic control rats (562.4±14.4), while the mean blood glucose level in treated diabetic rats was found significantly 92.69 mg/dl (AE) & 97.6 mg/dl (EE) (P<0.05) low as compared to diabetic rats (171.4 mg/dl). Our study concluded that presence of varieties of phytochemicals in FR provided strong antidiabetes potential.

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Formulation and evaluation of herbal tablet containing Terminalia chebula extract

Letters in Applied NanoBioScience ◽

10.33263/lianbs84.692697 ◽

2019 ◽

Vol 8 (4) ◽

pp. 692-697 ◽

Cited By ~ 1

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

International Diabetes Federation ◽

Diabetic Rats ◽

Antidiabetic Activity ◽

Albino Rats ◽

Terminalia Chebula ◽

Disintegration Time ◽

Weight Variation

According to WHO, the prevalence of diabetes is likely to increase by 35%. Currently there are over 150 million diabetics worldwide and this is likely to increase to 300 million or more by the year 2025. International Diabetes Federation (IFD) estimates the total number of diabetic subjects to be around 40.9 million in India and this if further set to rise to 66.9 million by the 2025. In view of the above discussion this study was undertaken to investigate antidiabetic activity of herbal tablet of Terminalia chebula in alloxan induced diabetic rats. Solid pharmaceutical dosage formulations using a novel dry plant extract (Terminalia chebula fruits) using various excipients i.e.carbopol, lactose, gelatin, magnesium stearate and dicalcium phosphate by the wet granulation was reported to the statically significant. The present communication deals with the evaluation of formulated tablets (weight variation, friability and hardness and disintegration time). Diabetes was induced in Wistar albino rats (170-200g) by a single dose (I.P.) of alloxan monohydrate (150mg/kg) dissolved in normal saline, treatment were given orally for 21days and blood glucose level was estimated on Two different batches of herbal tablets of Terminalia chebula extract (carbopol & gelatin) were studied for blood glucose level in two different groups of animals. Oral administration of Terminalia chebula tablet having carbopol to diabetic rats at a dose of 200mg/kg body weight to wistar rats in a significant reduction in biochemical parameters in alloxan diabetic rats, and the best formulation according to disintegration time. Thus our investigation clearly shows that the Terminalia chebula tablet has antidiabetic effects.

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Antidiabetic activity of herbal green tea extract from white mangrove (Avicennia marina) leaves towards blood glucose level of diabetic wistar rats (Rattus novergicus)

International Journal of Food Studies ◽

10.7455/ijfs.v8i2.553 ◽

2019 ◽

Vol 8 (2) ◽

Author(s):

Hardoko Hardoko ◽

Binti Nafi’ah ◽

Bambang B. Sasmito ◽

Yuniwaty Halim

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Green Tea ◽

Glucose Level ◽

Avicennia Marina ◽

Diabetic Rats ◽

Green Tea Extract ◽

Antidiabetic Activity ◽

White Mangrove ◽

Tea Extract

Green tea Camellia sinensis contains polyphenol that has antidiabetic activity. Mangrove leaves also contain polyphenol which potentially gives these leave antidiabetic activity. The aim of this research was to determine the ability of herbal green tea extract from white mangrove (Avicennia marina) leaves to decrease blood glucose level of diabetic Wistar rats (Rattus novergicus). The method used was experimental and involved giving a herbal green tea extract from white mangrove leaves with concentration of 100, 200 and 300 mg/200g BW/day, and positive control, i.e. glybenclamid (0.09 mg/200 g BW/day), to diabetic rats injected with Streptozotocin (STZ) and Nicotinamide (NA). The rats were observed on day 0, 5, 10 and 15. The results showed that the herbal green tea extract from white mangrove leaves decreased the blood glucose level of diabetic rats. The effective extract dose that decreased the blood glucose level of diabetic rats was 300 mg/200 g BW, which is comparable to the effect produced by glybenclamid (antidiabetic medicine). This dose could decrease the blood glucose level of diabetic rats to reach a normal blood glucose level after 20 days.

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Squalene decreased fasting blood glucose level of type ii diabetic rats

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/912/1/012088 ◽

2021 ◽

Vol 912 (1) ◽

pp. 012088

Author(s):

T Widyawati ◽

S Syarifah ◽

I B Sumantri

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Fasting Blood Glucose ◽

Diabetic Control ◽

Diabetic Rats ◽

High Dose ◽

Type Ii ◽

Fasting Blood Glucose Level ◽

Group I

Abstract Squaleneis a chemical compound that has been reported to have antidiabetic activity. The present study aimed to investigate the effect of squalene on fasting blood glucose level (FBGL) in type 2 diabetic rats. Diabetes type II in rats was obtained by giving nicotinamide (120 mg/kg) before high dose streptozotocin (60 mg/kg) intraperitoneally. A total of 18 diabetic rats were divided into 3 groups and served once daily for 12 days as follows; Group I Aquades (Diabetic Control) 10 ml/kg, Group II (Metformin 45 mg/kg) and Group III (Squalene 160 mg/kg). FBGL was measured at day 0, day 6 and day 12. The results showed that FBGL in both Squalene- (194.67 ± 28.32 mg/dL) and Metformin- (178.50 ± 34.27 mg/dL) were significantly decreased after 12 days intervention compared to Diabetic Control-treated groups (438.33 ± 65.79 µmol/L) with p<0.001. This study concluded that squalene was able to decrease FBGL in type II diabetic rats.

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PENGENDALIAN KADAR GLUKOSA DARAH OLEH TEH HIJAU DAN ATAU TEH DAUN MURBEI PADA TIKUS DIABETES

Jurnal Gizi dan Pangan ◽

10.25182/jgp.2010.5.2.87-94 ◽

2010 ◽

Vol 5 (2) ◽

pp. 87

Author(s):

Rusman Efendi ◽

Evy Damayanthi ◽

Lilik Kustiyah ◽

Nastiti Kusumorini

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Green Tea ◽

Glucose Level ◽

Diabetic Rats ◽

Feed Consumption ◽

Font Size ◽

High Prevalence ◽

The Difference ◽

Control Diabetes

Diabetes mellitus is degeneratif disease with high prevalence that happens in many countries. Several studies had been done to control diabetes by using green tea, mullberry leaf tea, and their mixture. The aim of this research was to analyze the influence of the administration green tea, mullbery leaf tea, and their mixtures to blood glucose level of diabetic rats both during 120 minutes after administration. This research had four phases, first to determine the best mullberry leaf tea, second to fourth phases respectively, determine turnover of blood glucose level on normal rats; attempt during 120 minutes on diabetic rats. The result of research during 120 minutes have showed that blood glucose level on diabetic rats which were administered by green tea, mullberry leaf tea and their mixture is significantly difference with diabetic rats which were administered by water. Blood glucose level at baseline increased at 30th minutes and showed the difference significantly and then until 60th and 120th minutes and relatively stable. During 120 minutes after feed consumption, inhibition of blood glucose level occured increasingly on diabetic rats which were administered by green tea, mullberry leaf tea, and their mixture compared to diabetic rats which were administered by water.

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Anti-diabetic activity of diphenhydramine in diabetic rats

International Journal of Research in Pharmaceutical Sciences ◽

10.26452/ijrps.v11i4.3102 ◽

2020 ◽

Vol 11 (4) ◽

pp. 5067-5070

Author(s):

Pang Jyh Chayng ◽

Nurul Ain ◽

Kaswandi Md Ambia ◽

Rahim Md Noah

Keyword(s):

Blood Glucose ◽

Blood Glucose Level ◽

Glucose Level ◽

Fasting Blood Glucose ◽

Insulin Level ◽

Diabetic Rats ◽

Group Iv ◽

Diabetic Rat ◽

Control Group ◽

Group I

The purpose of this project is to study the anti-diabetic effect of on a diabetic rat model. A total of Twenty male Sprague rats were used and it randomly distributed into four groups which are Group I: , Group II: negative control, Group III: and Group IV: and . In diabetic model were induced with via injection at the dosage of 65mg/kg. and FBG (Fasting Blood Glucose) level of diabetic rats were assessed every three days. Blood was collected via cardiac puncture at day 21 after the induction of treatment. Insulin level of the rats was assessed with the Mercodia Rat Insulin ELISA kit. FBG level of group I (12.16 ±3.96, p<0.05) and group IV (11.34 ±3.67, p<0.05) were significantly decreased. Meanwhile, the for all rats did not show any significant increase. However, the insulin level was escalated in group IV (0.74+0.25, p<0.05) significantly. The present study shows that the and the combination of and lowered blood glucose level and enhanced insulin secretion.

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