Minimum Inhibitory Concentration of Marine Microalgae Dunaliella salina on Fish Pathogenic Bacteria Edwardsiella tarda

Natural products are an important source of antibacterial agents. Canthin-6-one alkaloids have displayed potential antibacterial activity based on our previous work. In order to improve the activity, twenty-two new 3-N-benzylated 10-methoxy canthin-6-ones were designed and synthesized through quaternization reaction. The in vitro antibacterial activity against three bacteria was evaluated by double dilution method. Four compounds (6f, 6i, 6p and 6t) displayed 2-fold superiority (minimum inhibitory concentration (MIC) = 3.91 µg/mL) against agricultural pathogenic bacteria R. solanacearum and P. syringae than agrochemical propineb. Moreover, the structure–activity relationships (SARs) were also carefully summarized in order to guide the development of antibacterial canthin-6-one agents.

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Daya Antibakteri Ekstrak Daun Api-Api (Avicennia alba) terhadap Vibrio harveyi Penyebab Vibriosis secara Invitro [In Vitro Antibacteria Activity of Api-Api (Avicennia alba) Leave Extract Against Vibrio harveyi Causes Vibriosis]

Jurnal Ilmiah Perikanan dan Kelautan ◽

10.20473/jipk.v10i2.10527 ◽

2018 ◽

Vol 10 (2) ◽

pp. 131

Author(s):

Zurica Melati Fitri ◽

Kismiyati Kismiyati ◽

Ahmad Shofy Mubarak

Keyword(s):

Minimum Inhibitory Concentration ◽

Experimental Method ◽

Pathogenic Bacteria ◽

Inhibitory Concentration ◽

Vibrio Harveyi ◽

Colony Growth ◽

Minimum Concentration ◽

Extract Concentration ◽

Avicennia Alba

AbstrakBudidaya udang dihadapkan pada berbagai kendala penyakit yang timbul dan dapat menyebabkan kematian masal pada larva udang windu, salah satunya vibriosis yang disebabkan oleh bakteri Vibrio harveyi. Zat antibakteri yaitu : flavonoid, saponin dan tanin terbukti dapat membunuh beberapa bakteri patogen. Daun api – api Avicennia alba mengandung zat anti bakteri sehingga berpotensi digunakan untuk membunuh bakteri Vibrio harveyi. Penelitian ini bertujuan untuk mengetahui konsentrasi optimum ekstrak daun api-api (Avicennia alba) dalam menghambat dan membunuh pertumbuhan bakteri Vibrio harveyi. Penelitian ini mengunakan metode eksperimental dengan perlakuan konsentrasi ekstrak daun api-api (Avicennia alba) sebesar 100%, 90% 80%, 70%, 60%, 50%, 40%. 30%, 20%, 10%, 0%. Parameter yang diamati dalam penelitian ini adalah Minimum Inhibitory Concentration (MIC) dan pertumbuhan koloni pada uji Minimum Bacteria Concentration (MBC). Hasil penelitian menunjukan bahwa pada konsentrasi 60% ekstrak daun Avicennia alba dapat menghambat pertumbuhan V.harveyi. Sedangkan pada konsentrasi 90% ekstrak daun Avicennia alba menyebabkan bakteri V.harveyi terbunuh. AbstractShrimp culture has various obstacles such as the emergence of diseases that can cause deaths in tiger shrimp larvae such as vibriosis caused by Vibrio harveyi. Some antibacterial substances such as flavonoid, saponin, and tannin inhibit and kill several pathogenic bacteria. Avicennia alba leaves contain anti-bacterial substances that have the potential to be used to kill Vibrio harveyi bacteria. This study aimed to determine the minimum concentration of Avicennia alba leaves extract for inhibiting and kill the growth of Vibrio harveyi. This study used an experimental method, using Avicennia alba leaves extract concentration treatment of 100%, 90% 80%, 70%, 60%, 50%, 40%. 30%, 20%, 10%, 0%. The parameters observed in this study were the Minimum Inhibitory Concentration (MIC) and bacteria colony growth in Minimum Bacteria Concentration (MBC) test. The results showed that at a concentration of 60% Avicennia alba leaves extract could inhibit the growth of V.harveyi. While at a concentration of 90% Avicennia alba leaves extract, Vibrio harveyi bacteria was killed.

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STUDY CYTOTOXICITY, ANTIBACTERIAL ACTIVITY, AND COMPOSITIONS BY FT-IR AND GC-MS FOR ORGANIC EXTRACT OF WHITE IRAQI DESERT TRUFFLE TIRMANIA NIVEA

Periódico Tchê Química ◽

10.52571/ptq.v17.n36.2020.34_periodico36_pgs_18_31.pdf ◽

2020 ◽

Vol 17 (36) ◽

pp. 18-31

Author(s):

Ahmad khadem HACHIM ◽

Rashid Rahim HATEET ◽

Tawfik Muhammad MUHSIN

Keyword(s):

Antibacterial Activity ◽

Minimum Inhibitory Concentration ◽

Cell Line ◽

Pathogenic Bacteria ◽

Inhibitory Concentration ◽

Biological Activities ◽

Gas Chromatography Mass Spectrometry ◽

Bacterial Strains ◽

Organic Extract

The purpose of the present work aimed at exploring the potential biochemical components and biological activities of an organic extract of the white truffle Tirmania nivea collected from the Iraqi desert, then test the organic extract against the Cytotoxicity on Human Larynx carcinoma cells and selected strains of pathogenic bacteria. Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectrometry GC/MSS were used to analyze mycochemical compositions. The antibacterial activity and Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) was investigated using a disk diffusion agar method. The truffle extract's cytotoxicity effect against the larynx cell line (Hep-2) was assessed by the MTT assay (in vitro). FTIR results provided the presence of phenol, carboxylic acid, and alkane's functional group, The GC-MS analysis of T. nivea disclose the existence of nineteen compounds that can contribute to the pharmaceutical properties of the truffle. As for antibacterial activity result, A growth inhibition activity of truffle extract at (18-40 mm inhibition zones) against the tested pathogenic bacterial strains was detected, which minimum inhibitory concentration values ranged from 3.12 to 6.25 mg/mL for Escherichia coli (ATCC 25922) and Staphylococcus aureus (ATCC 25923) Respectively. The results of cytotoxicity shown that the organic truffle extract exhibited a high inhibitory rate (52.685%) against cell line (Hep-2) at a concentration of 1.56 ?g/mL. In this work, the results showed that the organic extracts of T. nivea are very promising as cancer cytotoxicity and antibacterial agent for future medical applications.

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SENSITIVITY OF PINEAPPLE PEEL (Ananas comosus) EXTRACT AGAINSTEdwardsiella tarda BACTERIA

Jurnal Ruaya Jurnal Penelitian dan Kajian Ilmu Perikanan dan Kelautan ◽

10.29406/jr.v8i1.1847 ◽

2020 ◽

Vol 8 (1) ◽

Author(s):

Muhammad Azaldin ◽

Henni Syawal ◽

Iesje Lukistyowati

Keyword(s):

Minimum Inhibitory Concentration ◽

Inhibitory Concentration ◽

Edwardsiella Tarda ◽

Ananas Comosus ◽

Pineapple Peel

ABSTRAK Kulit nanas (Ananas comosus) merupakan limbah organik yang dapat dimanfaatkan sebagai antimikroba / antibakteri karena mengandung senyawa metabolit sekunder, seperti flavonoid, tanin, dan saponin. Edwardsiella tarda adalah patogen yang dapat menimbulkan penyakit pada ikan budidaya. Penelitian ini bertujuan untuk mengetahui sensitivitas dari ekstrak kulit nanas (A.comosus) dalam menghambat pertumbuhan E.tarda dan Minimum Inhibitory Concentration (MIC). Konsentrasi ekstrak kulit nanas yang digunakan adalah 100%-10% dan sebagai kontrol antibiotik oxytetracycline. Hasil penelitian menunjukkan bahwa sensitivitas dari kulit nanas terhadap E. tardaberkisar antara 6,13 mm - 14,78 mm dan pada konsentrasi 18% ditetapkan sebagai nilai MIC dengan kepadatan bakteri 265,33 CFU/mL. Kata Kunci : Ekstrak Kulit Nanas(Ananas comosus), Edwardsiella tarda, Sensitivitas, Minimum Inhibitory Concentration

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Antibacterial Properties of Local Malaysian Trigona Sp. Honey Towards Different of Pathogenic Bacteria in Vitro

Diyala Agricultural Sciences Journal ◽

10.52951/dasj.21130101 ◽

2021 ◽

Vol 13 (1) ◽

pp. 1-9

Author(s):

Fawzia Jassim Shalsh ◽

Ahmed Mohammed Khalaf ◽

Mohammed Hafiz ◽

Imad Ibrahim Ali

Keyword(s):

Minimum Inhibitory Concentration ◽

Pathogenic Bacteria ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

Antibacterial Properties ◽

Antimicrobial Properties ◽

Therapeutic Effects ◽

Disc Diffusion ◽

Diffusion Test

Honey is one of the oldest natural medicines known with a very high therapeutic value. Nowadays, in the medical field, several important therapeutic effects of honey have been elucidated. This study was conducted to reveal the antimicrobial activity of the commercially available local Malaysian Trigona sp. honey towards different pathogenic bacteria specifically Staphylococcus aureus, Streptococcus pyogenes, Escherichia coli, and Salmonella typhimurium. The Minimum inhibitory concentration and Minimum bactericidal concentration were determined by the disc-diffusion test and agar well diffusion test. Different concentration of the honey was tested in the disc-diffusion and agar well diffusion test. The results of these tests were in terms of Inhibition zone diameter. The results obtained from the current study are the dilution of different concentration of honey from Trigona sp. are very significant because the only net concentration of both of honey Trigon a sp. possessed antimicrobial properties in term of Minimum inhibitory concentration) and Minimum bactericidal concentration. The result also can say that Trigona sp. honey possessed antibacterial properties and can be used as alternative medicine in the veterinary field in the future.

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Isolation and Characterization of Lactic Acid Bacteria From Milk and Their Effects on the Pathogenic Bacteria

Research in Molecular Medicine ◽

10.32598/rmm.8.4.4 ◽

2020 ◽

Vol 8 (4) ◽

pp. 189-200

Author(s):

Ciamak Ghazaei ◽

Keyword(s):

Lactic Acid ◽

Minimum Inhibitory Concentration ◽

Lactic Acid Bacteria ◽

Pathogenic Bacteria ◽

Inhibitory Concentration ◽

Inhibitory Effect ◽

Cow Milk ◽

Milk Samples ◽

Isolation And Characterization ◽

Antibiotic Susceptibility Test

Background: Probiotics are “live microbial cells” that are beneficial for human and animal health. Lactobacilli are such a diverse group of bacteria with similar metabolic and physiological characteristics, and constitute important and beneficial gut microflora. During carbohydrate fermentation, lactobacilli produce lactic acid as an end product in metabolism. Hence, lactobacilli have high significance to be used as probiotics in the food industry, because of their acidifying properties. Also, lactobacilli are considered “safe”, owing to their ubiquitous presence in the food. Many researchers provided evidence for the presence of lactobacilli in milk sources. Thus, the present study aimed to isolate and characterize different lactobacilli strains from milk sources and analyze their “probiotic potential”. Materials and methods: Forty-one lactobacilli isolates were obtained from raw cow milk. Then, the strains were characterized by morphological identification and biochemical tests. Besides, probiotic potentials were evaluated with the bile tolerance test, antibiotic susceptibility test, and determining suitable pH for the optimal growth of lactobacilli. The lactobacilli isolates were also analyzed for their probiotic characteristics and the release of antimicrobial substances. Their antimicrobial activities against pathogenic strains were assessed by determining the minimum inhibitory concentration, with the help of agar diffusion methods. Results: From 50 milk samples, 41 lactobacilli isolates were obtained, out of which five lactobacilli strains were identified as Lactobacillus casei, Lactobacillus plantarum, Lactobacillus brevis, Lactobacillus acidophilus, and Lactobacillus lactis. Moreover, 35 isolates showed an inhibitory effect. These strains were able to survive and grow in 0.5% to 2.5% bile salt concentrations. Lactic acid bacteria were susceptible to antibiotics, and 35 isolates obtained from raw milk showed an inhibitory effect against pathogenic bacteria. The observed minimum inhibitory concentration ranged from 50 to 100 µL and varied between the different pathogens. Conclusion: Out of 41 Lactobacillus isolates obtained from cow milk samples, 35 were identified with probiotic characteristics. Hence, this study highlighted the novel probiotic bacteria and validated the antimicrobial properties of the Lactobacillus spp against pathogenic bacteria.

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Antibacterial and Cytotoxic Activity of Longiverbenone Isolated from the Rhizome of Cyperus scariosu

Bangladesh Journal of Microbiology ◽

10.3329/bjm.v25i1.4866 ◽

1970 ◽

Vol 25 (1) ◽

pp. 82-84 ◽

Cited By ~ 1

Author(s):

M Shafiqur Rahman ◽

M Nural Anwar

Keyword(s):

Antibacterial Activity ◽

Minimum Inhibitory Concentration ◽

Cytotoxic Activity ◽

Brine Shrimp ◽

Pathogenic Bacteria ◽

Inhibitory Concentration ◽

Ethanolic Extract ◽

Artemia Salina ◽

Diffusion Method ◽

Chromatographic Technique

Longiverbenone is a naturally occurring sesquiterpene isolated from ethanolic extract of Cyperus scariosus rhizome by solvent-solvent portioning and chromatographic technique. The antibacterial activity of longiverbenone was evaluated against eleven potential human pathogenic bacteria using disc diffusion method. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by broth macrodilution method. Cytotoxic activity (lethal concentration 50%, LC50) of longiverbenone was determined on new borne brine shrimp (Artemia salina). Longiverbenone showed moderate to good antibacterial activity against the test organisms tested herein. It exhibited the lowest MIC (20 μg/ml) and MBC (80 μg/ml) against Vibrio cholerae. The LC50 of the isolated sesquiterpene was found to be 14.38 μg/ml against new borne brine shrimp. Keywords: Antibacterial activity; Cytotoxic activity (LC50); Cyperus scariosu; Longiverbenone; Minimum inhibitory concentration (MIC)DOI: http://dx.doi.org/10.3329/bjm.v25i1.4866 Bangladesh J Microbiol, Volume 25, Number 1, June 2008, pp 82-84

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Characterization and partial purification of an antibacterial agent from halophilic actinomycetes Kocuria sp. strain rsk4

Bioimpacts ◽

10.15171/bi.2018.28 ◽

2018 ◽

Vol 8 (4) ◽

pp. 253-261 ◽

Cited By ~ 5

Author(s):

Ravi Ranjan Kumar ◽

Vasantba J Jadeja

Keyword(s):

Minimum Inhibitory Concentration ◽

Broad Spectrum ◽

Antibacterial Agent ◽

Pathogenic Bacteria ◽

Inhibitory Concentration ◽

Flash Chromatography ◽

Resistant Bacteria ◽

Antibiotic Resistant ◽

Antibacterial Compound ◽

Halophilic Actinomycetes

Introduction: The inevitable rise of antibiotic-resistant bacteria is a global health problem. These pathogens erode the utility of available antibiotics. Staphylococcus aureus is one of the major causes of community-acquired infections. The aim of work was to evaluate the marine actinomycetes for production of the antibacterial agent against pathogens. Methods: Halophilic actinomycetes were isolated, characterized and screened for production of antibacterial agent against pathogenic bacteria. The antibacterial compounds were extracted by solvent extraction and separated by TLC based bioautography. Antibacterial compound was further purified by flash chromatography followed by high-performance liquid chromatography (HPLC) techniques. The active fraction was characterized by spectroscopy techniques. The minimum inhibitory concentration of antibiotic was determined against pathogens. Results: A new halophilic actinomycetes strain rsk4 was isolated from marine water. It was designated as Kocuria sp. based on the physiological, biochemical and 16S rDNA sequence-based characters. It was able to produce broad-spectrum antibacterial compound and exhibited significant inhibitory activities against antibiotic-resistant S. aureus. The antibacterial compound was secreted optimally at 5% NaCl and neutral pH in the starch casein medium during stationary phase. The crude ethyl acetate extract was separated by chloroform-methanol, 24:1, v/v having Rf value 0.45. Bioassay of HPLC fractions confirms the presence of antibiotics picks at retention time: 3.24 minutes. The UV-Visible and mass spectra of the compound revealed that the active compound was different from other known antibiotics. The lowest minimum inhibitory concentration was recorded against S. aureus (30 µg/mL). Conclusion: The result suggests that a broad-spectrum antibacterial compound obtained from halophilic actinomycetes is effective against pathogenic bacteria. This compound may be a good alternative treatment against antibiotic-resistant pathogen S. aureus.

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Antibacterial Activities of Some Schiff Bases Involving Thiosemicarbazide and Ketones

International Letters of Chemistry Physics and Astronomy ◽

10.18052/www.scipress.com/ilcpa.26.53 ◽

2014 ◽

Vol 26 ◽

pp. 53-61 ◽

Cited By ~ 1

Author(s):

S.M.S. Shariar ◽

M. Jesmin ◽

M.M. Ali

Keyword(s):

Antibacterial Activity ◽

Minimum Inhibitory Concentration ◽

Schiff Bases ◽

Cytotoxic Effect ◽

Pathogenic Bacteria ◽

Inhibitory Concentration ◽

Antibacterial Activities ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method

Three Schiff bases derived from thiosemicarbazide and ketones (Vanillin, Benzophenone and Acetophenone) were used to study their antibacterial activities against some pathogenic bacteria by disc diffusion method. Of these, benzophenone thiosemicarbazone showed significant antibacterial activity as compared with that of Kanamycin. All these three compounds were found to possess cytotoxic effect. Minimum inhibitory concentration of these compounds was also determined.

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