scholarly journals The Role of Endothelial Dysfunction Markers in Pregnant Women with Chorion Detachment, Included in the Program of Auxiliary Reproductive Technologies

2017 ◽  
Vol 24 (3) ◽  
Author(s):  
Natalya Lytvyn
Keyword(s):  
Endothelial Dysfunction ◽  
Pregnant Women ◽  
Reproductive Function ◽  
Reproductive Technologies ◽  
The Body ◽  
Control Group ◽  
Vitro Fertilization ◽  
Endothelin 1

An urgent medical and social problem is the restoration of reproductive function of womenwho suffer from infertility, which became possible due to auxiliary reproductive technologies. Women with induced pregnancy make thegroup of a high-risk on miscarriage, due to interrelated processes –immunological disorders and endothelial dysfunction that occur in the body of pregnant women after the use of extracorporal fertilization programs, and can lead to the chorion detachment and the formation of subchorionic hematomas.The purpose of the study is to determine the role of endothelial dysfunction as one of the leading factors that determine the development of a local non-progressive chorion detachment in infertile patients included in the program of auxiliary reproductive technologies.Materials and methods. We have examined 130 pregnant women, who were divided into groups: the control group included 30 women, whose pregnancy occurred in the natural cycle and with uncomplicated gestational course; the main group – 50 patients with induced pregnancy and risk factors of the occurrence of chorion detachment, who wereperformed the proposed pre-gravidapreparation; the comparative group – 50 pregnant women who received a standard scheme of pregnancy management before and after in-vitro fertilization. A general clinical examination, ultrasound examination, homocysteine level determination, endothelin-1 and nitrogen oxide metabolites were performed.Results. In women included into the program of auxiliary reproductive technologies with local chorion detachment were recorded changes of vascular endothelial function with a possible increase in endothelin-1 production and a decrease of the nitric oxidesynthesis. During the induced pregnancy with the presence of subchorionic hematoma, an increase of the level of endothelium-damaging factor of homocysteine was noted.Conclusions.This study identifies the parameters that reflect the main links of endothelial dysfunction and can be used as markers of local chorion detachment.

Peculiarities of pregnancy and delivery course in women after the application of assisted reproductive technologies against obesity (Based on the retrospective analysis)

2018 ◽  
pp. 122-126
Author(s):  
I.A. Zhabchenko ◽  
◽  
O.R. Sudmak ◽  
Keyword(s):  
In Vitro Fertilization ◽  
Pregnant Women ◽  
Retrospective Analysis ◽  
Perinatal Outcomes ◽  
Reproductive Technologies ◽  
Ischemic Lesion ◽  
Vitro Fertilization ◽  
Pregnancy And Delivery

The objective: to study the structure and frequency of complications of pregnancy, deliveries and perinatal outcomes in three groups of women: women with infertility and obesity, treated by application of in vitro fertilization (hereinafter IVF), pregnant women after IVF application with normal body weight, and pregnant women on the background of obesity which did not have an infertility in past history. Materials and methods. A retrospective analysis of 221 case histories of pregnancies and labors in women who were treated and gave birth in the Pregnancy and delivery pathology Department of SI «Institute of Pediatrics, Obstetrics and Gynecology named after Acad. O. M. Lukyanova of NAMS of Ukraine» for 2012 – 2016 years was carried out. Results. The overwhelming majority of pregnant women after IVF on the background of obesity are primaparas, who have a complicated obstetric history, hormonal changes in the form of progesterone deficiency predominantly and chronic inflammatory processes. Pregnancy with a combination of infertility, treated by the means of IVF application, and obesity, in most cases is accompanied by a long-term threat of termination of pregnancy (48.8%), threatening preterm deliveries (56%), placental dysfunction (41.5%), premature rupture of the amniotic membranes (41.5%), other problems during pregnancy, at the same time, every second woman (58.5%) had a combination of several complications, and required a long-term and repeated inpatient treatment (53.7%). The specific gravity of surgical delivery was 90%, and 16.2% of such deliveries were complicated by pathological blood loss. The number of preterm deliveries was 17.1%, with perinatal losses up to 11.3‰. Among full-term newborns 21.3% of newborns had malnutrition of the I degree and 17% of them had hypoxic-ischemic lesion of CNS. Conclusion. The course of pregnancy, delivery and the postpartum period in the studied contingent of women has a significant frequency of complications, mainly the coinciding ones, which affects on the consequences of perinatal outcomes and requires further study of this problem and the development of differentiated algorithms for antenatal observation. Key words: pregnancy, obesity, in vitro fertilization, complications, delivery, newborn.

scholarly journals IN VITRO FERTILITY TEST OF HUMAN SPERMATOZOA MEMBRANE PROTEIN FERTILIN BETA ANTIBODY IN MICE (Mus musculus Balb/c) AS IMMUNOCONTRACEPTIVE CANDIDATE

2017 ◽  
Vol 52 (3) ◽  
pp. 209
Author(s):  
Reny I’tishom ◽  
Doddy M Soebadi ◽  
Aucky Hinting ◽  
Hamdani Lunardhi ◽  
Rina Yudiwati
Keyword(s):  
In Vitro Fertilization ◽  
Membrane Protein ◽  
Mus Musculus ◽  
Human Spermatozoa ◽  
Control Group ◽  
Reproductive Process ◽  
Vitro Fertilization ◽  
Antibody Induction

One of the materials as potential candidates immunocontraception material is spermatozoa. Fertilin beta is spermatozoa membrane protein and is found only in mature spermatozoa and ejaculate, which serves as an adhesion molecule. Spermatozoa membrane protein that is used as an ingredient immunocontraception candidate, must have specific criteria that the specificity of spermatozoa, the role of antigen in the fertilization process, which includes the formation of immunogenicity sufficient antibody response has the potential to block fertilization. Antibodies against spermatozoa affect the stages before fertilization of the reproductive process and can hinder the development of the embryo after fertilization. Until now very little research data spermatozoa membrane protein as an ingredient immunocontraception are up to the test of experimental animals. The research objective is to prove the role of the resulting antibody induction of antibodies fertilin beta protein in the membrane of human spermatozoa induce agglutination and reduce motility thus reducing the number of in vitro fertilization. Research conducted at the IVF Laboratory, Department of Biology of Medicine, Faculty of Medicine, University of Airlangga. This research includes: Test the potential of antibody protein beta fertilin membrane of human spermatozoa and inhibit the role of antibodies in vitro fertilization in mice (Mus musculus Balb/c). In vitro studies have resulted in fertilization figure of 25% is smaller than the number that is equal to control fertilization of 58.7%, whereas previously the spermatozoa were incubated first with a beta membrane protein antibody fertilin human spermatozoa. While the percentage of inhibition of sperm to fertilize an oocyte by 33.75%. Potential imunokontraseptif considered effective if it decreased significantly (P <0.05) than the numbers fertilization in the treatment group compared with the control group. This shows fertilin beta membrane protein antibody has the ability to inhibit human spermatozoa to fertilize oocytes that reduce the number of fertilization.

scholarly journals The Role of Interleukin-18 in Serum and Follicular Fluid during In Vitro Fertilization and Intracytoplasmic Sperm Injection

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Veronika Günther ◽  
Ibrahim Alkatout ◽  
Corinna Fuhs ◽  
Ali Salmassi ◽  
Liselotte Mettler ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Follicular Fluid ◽  
Intracytoplasmic Sperm Injection ◽  
Ovarian Stimulation ◽  
The Body ◽  
Interleukin 18 ◽  
Vitro Fertilization ◽  
Median Level

Cytokines are key modulators of the immune system and play an important role in the ovarian cycle. IL-18 levels in serum and follicular fluid were analyzed in women undergoing in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI) treatment. The cohort study group consisted of 90 women, who were undergoing IVF or ICSI. The body mass index (BMI) was determined in all patients; IL-18 levels were measured in follicular fluid and serum. IL-18 levels in serum were significantly higher than those in follicular fluid. The median level in serum was 162.75 (80.21) pg/mL and that in follicular fluid, 138.24 (91.78) pg/mL. Women undergoing IVF treatment had lower IL-18 levels in serum (median, 151.19 (90.73) pg/mL) than those treated with ICSI (median, 163.57 (89.97) pg/mL). The correlation between IL-18 levels in serum and BMI was statistically significant, as well as the correlation between IL-18 levels in follicular fluid and ovarian stimulation response (p=0.003). IL-18 was correlated with the response to ovarian stimulation and was the reason for successful pregnancy after IVF or ICSI treatment. Among other cytokines, IL-18 appears to be a promising prognostic marker of success in reproductive treatment and should be evaluated as such in further prospective studies.

scholarly journals The effect of combined oral contraceptives and hormone therapy during assisted reproductive technologies on the function of the hemostatic system in non-pregnant and pregnant women

2020 ◽  
Vol 7 (1) ◽  
pp. 10-18
Author(s):  
E. V. Kirakosyan ◽  
E. A. Sosnova
Keyword(s):  
Pregnant Women ◽  
Hormone Therapy ◽  
Oral Contraceptives ◽  
World Literature ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Combined Oral Contraceptives ◽  
Vitro Fertilization ◽  
Hemostatic System

A review of 130 sources of world literature was conducted in the databases Scopus, Web of Science, MedLine, Cochrane CENTRAL, Cochrane Database of Systematic Reviews (CDSR), Database of Abstracts of Reviews of Effectiveness (DARE), EMBASE, Global Health, CyberLeninka, RSCI on the problem of the effect of combined oral contraceptives and hormone therapy during assisted reproductive technologies on the function of the hemostatic system in non-pregnant and pregnant women. In this review, we have summarized and analyzed information on the effect of sex hormones entering the womans body from the outside on individual links and the hemostatic system as a whole, determined the relationship between drug administration and thrombotic complications, optimized the sequence of diagnosis of conditions predisposing to thrombosis, substantiated the tactics of preparation and management patients taking exogenous hormones before and during pregnancy, including through in vitro fertilization.

Clinical and immunological aspects of treatment women with repeated in-vitro fertilization failure

2014 ◽  
Vol 63 (5) ◽  
pp. 89-95
Author(s):  
Marina Ivanovna Krivonos ◽  
Marina Sabirovna Zaynulina ◽  
Sergey Vladimirovich Chepanov ◽  
Alexandr Vasilevich Selutin ◽  
Sergey Alekseevich Selkov ◽  
...  
Keyword(s):  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Implantation Failure ◽  
Fertilization Failure ◽  
Vitro Fertilization ◽  
Repeated Implantation Failure ◽  
Assisted Reproductive Treatment ◽  
Immune Pathology

This review presents the existing literature concerning repeated implantation failure in assisted reproductive technologies. There are also discussed the role of immune pathology in the occurrence of repeated implantation failure in assisted reproductive treatment and the prospects of improving implantation rates with intravenous immunoglobulin in women with different immune pathologies.

Stateof-the-art of realization of the fertility potential in the women of late reproductive age

2016 ◽  
Vol 94 (1) ◽  
pp. 10-15
Author(s):  
Kristina S. Ermolenko ◽  
V. E. Radzinsky ◽  
S. I. Rapoport
Keyword(s):  
In Vitro Fertilization ◽  
Reproductive Technologies ◽  
Reproductive Age ◽  
Vitro Fertilization ◽  
Biological Potential ◽  
Reproductive Material ◽  
Seasonal Biorhythms

Biological potential of childbearing in the women of late reproductive age is limited by natural impairment and loss of fertility. Despite a considerable progress in clinical application of new diagnostic and reproductive technologies, the problem of infertility remains a most serious challenge. Women’s age is one of the main factors responsible for the outcome of in vitro fertilization. The low effectiveness of in vitro fertilization programs is attributed to discoordination in the hypothalamic-pituitary system, depletion of ovarian resources, and deterioration of quality of reproductive material. Bearing in mind the role of melatonin in synchronizing circadian and seasonal biorhythms and regulating physiological and pathological processes, it is natural to suggest its role in the enhancement of efficiency of in vitro fertilization programs for women of late reproductive age.

The combination of polymorphisms of genes PAI-1 -675 5G>4G, FXIII: Val34Leu G>T and ITGA2: 807 C>T in women with infertility can be considered as a predictor of failures of assisted reproductive technologies

2019 ◽  
Author(s):  
С.И. Сафиуллина ◽  
Я.Н. Котова ◽  
Е.С. Ворошилина ◽  
Н.А. Илизарова ◽  
Л.Ш. Ягудина ◽  
...  
Keyword(s):  
Assisted Reproductive Technologies ◽  
Treatment Effectiveness ◽  
Risk Groups ◽  
Reproductive Technologies ◽  
Fertility Treatment ◽  
Control Group ◽  
Vitro Fertilization ◽  
Ivf Failure ◽  
Pai 1

Введение. Результативность программ вспомогательных репродуктивных технологий остается неизменно низкой и не превышает 40 по числу положительных результатов хорионического гонадотропина человека и 23 по коэффициенту рождаемости. Актуальны новые способы увеличения эффективности лечения методом экстракорпорального оплодотворения (ЭКО) и вынашивания наступившей беременности. Цель исследования: на основании исследования генов полиморфизмов системы гемостаза выделить группы риска неудачных исходов программ ЭКО у женщин с бесплодием. Материалы и методы. Изучена когорта 130 женщин, планирующих лечение бесплодия методом ЭКО, и 49 женщин группы контроля. У всех женщин исследованы наиболее распространенные полиморфизмы системы гемостаза: FV: 1691 GA, FII: 20210 GA, FXIII: Val34Leu GT, FGB: 455 GA, ITGA2: 807 СT, ITGB3: 1565 TC, PAI 1: 675 5G4G методом полимеразной цепной реакции, выполнено сравнение их распространенности с аналогичными показателями контрольной группы. Проанализированы частоты встречаемости и значимости изученных полиморфизмов в 140 протоколах с переносом эмбрионов в зависимости от исхода. Результаты. У женщин с бесплодием, планирующих проведение программы ЭКО, не обнаружено достоверных различий в частоте распространенности изученных полиморфизмов системы гемостаза по сравнению с контрольной группой. Установлена достоверно высокая частота распространения триады полиморфизмов PAI1: 675 4G/4G, ITGA2: 807 СT и FХIII: Val34Leu GT у женщин с отрицательными исходами программы ЭКО по сравнению с положительными исходами. Заключение. Перспективно выделение группы риска неудач ЭКО на основании результатов генетического тестирования полиморфизмов системы гемостаза. Introduction. The effectiveness of assisted reproductive technology programs remains consistently low and does not exceed 40 in the number of positive results of human chorionic gonadotropin and 23 in terms of the birth rate. New ways of increasing the treatment effectiveness with in vitro fertilization (IVF) and carrying the new pregnancy are actual. Aim: to identify risk groups of IVF unsuccessful outcomes in women with infertility by studying of hemostasis genes polymorphisms. Materials and methods. We examined 130 women planning fertility treatment using IVF and 49 women as a control group. In all women we studied the most common hemostasis polymorphisms: FV: 1691 GA, FII: 20210 GA, FXIII: Val34Leu GT, FGB: 455 GA, ITGA2: 807 СT, ITGB3: 1565 TC, PAI1: 675 5G4G by polymerase chain reaction, and compared their prevalence with similar parameters of the control group. We analyzed the frequency of occurrence and significance of studied polymorphisms in 140 protocols with embryo transfer in dependence to outcome. Results. In women with infertility planning IVF program, there were no significant differences in the prevalence rate of studied hemostasis polymorphisms in comparison with the control group. We revealed significantly high frequency of 3 polymorphisms occurrence PAI1: 675 4G/4G, ITGA2: 807 CT and FХIII: Val34Leu GT in women with negative outcomes of IVF program in comparison with positive outcomes. Conclusion. Identification of risk groups of IVF failure based on the results of genetic testing of hemostasis polymorphisms is promising.

scholarly journals Auxiliary reproductive technologies in the samara region: efficiency, complications, perspective strands of work

2020 ◽  
Vol 20 (1-2) ◽  
pp. 19-27
Author(s):  
Mikhail A. Ratmanov ◽  
Armen S. Benyan ◽  
Ludmila S. Tshelkovich ◽  
Olga V. Tumina ◽  
Regina B. Balter ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Pregnancy Rate ◽  
Reproductive Function ◽  
Scientific Work ◽  
Correct Choice ◽  
Reproductive Technologies ◽  
Clinical Pregnancy ◽  
Clinical Pregnancy Rate ◽  
Vitro Fertilization

The paper studies the performance of Samara fertility specialists. It is noted that in 2019 the Samara Region conducted twice as many successful IVF cycles as compared to 2012, when it was 1840 cycles. The clinical pregnancy rate in the region is 47%, which is significantly higher than the same indicator of 38.5% in Russia. The trends of scientific work of reproductologists in Samara are presented in the article. The purposes of their work are the correct choice of the in vitro fertilization protocol, the prevention of complications of induced pregnancy, the restoration of reproductive function after unsuccessful IVF attempts.

scholarly journals CHRONIC ENDOMETRITIS AS A FACTOR OF FAILURE ATTEMPT IN ASSISTED REPRODUCTIVE TECHNOLOGIES

2019 ◽  
pp. 13-21
Author(s):  
O. V. Onysko ◽  
О. О. Korchynska ◽  
Stefania Andrashchikova ◽  
Sylvia Zhultakova ◽  
Alena Shlosserova
Keyword(s):  
Plasma Cells ◽  
Assisted Reproductive Technologies ◽  
Proteolytic Enzymes ◽  
Uterine Cavity ◽  
Reproductive Function ◽  
Reproductive Technologies ◽  
Careful Study ◽  
Chronic Endometritis ◽  
Vitro Fertilization

Increase in frequency of disorders of women's reproductive function justifies a careful study of the underlying pathological processes. In recent years, increased attention in solving fertility problems is paid to endometrial pathology when chronic endometritis plays an important role. Current worksarefeeding an in-depth study of the etiology and pathogenesis of chronic endometritis. However, results of research pose new questions with an imaginary comprehensive study of this problem. Chronic endometritis is one of the causes of infertility and may play a role in certain complications of pregnancy and childbirth (30.3 % in patients with repeated failures of in vitro fertilization, 9.3 % with recurrent miscarriages, 9.8 % in infertility). Clinically, chronic endometritis is often asymptomatic or accompanied by nonspecific symptoms such as pelvic pain, dyspareunia, abnormal uterine bleeding, and discharges. Previously, scientists considered the uterine cavity as a sterile container. But a team of Spanish scientists sought to test for the presence of intrauterine microflora, which differs from the vaginal. As a result, the study of endometrial fluid and vaginal samples from the same patients revealed different bacterial compositions. Microflora in the intrauterine fluid was classified as Lactobacillus, and women with non-lactobacillary flora of the endometrium had a significantly lower frequency of implantation. At the initial stage of endometrial inflammation is an activation of neutrophils and macrophages, increased cytokines synthesis, extracellular matrix degradation by proteolytic enzymes. Disorders of microcirculation and sclerotic processes in the area of ​​injury lead to the ischemia and tissue hypoxia, which activates the processes of sclerosis and angiogenesis. Chronization of the process increases the expression of chemokines and adhesion molecules, that is lead to migration of B-lymphocytes from the bloodstream and their differentiation into plasma cells, increases the activity of matrix metalloproteinases, changes local expression of estrogen and progesterone receptors. Lack of a pronounced specific clinical picture of chronic endometritis, its later detection causes impaired reproductive function. Untimely diagnosis and treatment of chronic endometritis has significant consequences in terms of the possibility of implantation of a fertilized egg in the programs of assisted reproductive technologies.

scholarly journals Possible Retard in the Language Development to the Children Born through IVF (In Vitro Fertilisation)

2019 ◽  
Vol 70 (5) ◽  
pp. 1859-1858
Author(s):  
Ramona Nedelcuta ◽  
Vlad Dumitru Baleanu ◽  
Cosmin Ciora ◽  
Gigi Calin ◽  
Mihail-Relu Stanescu ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Breast Milk ◽  
Language Development ◽  
In Vitro Fertilisation ◽  
Control Group ◽  
Nerve Growth ◽  
Vitro Fertilization ◽  
Natural Way

Research in recent years on a possible and evident pathology, appeared in children s case from pregnancies obtained through in vitro fertilization (IVF), did not show significant differences than the children born from pregnancies obtained naturally. Having a good start in life, the consignments studied were feed exclusively in a natural way in the first 6 months, knowing the complex role of breast milk, including nerve growth. The delay in language development has shown significant differences between the IVF consignment and the control group and the pathology exam a possible involvement in cerebral embryogenesis, especially the Broca s area, of some disturbing factors, with a maximum of action before the 23rd week.

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