scholarly journals Isolation of bacteria present in the veterinary necropsy room and health risks

2021 ◽  
Vol 27 (1) ◽  
pp. e2021
Author(s):  
Isabela Fernanda Spinelli Perossi ◽  
Paulo Baptista Martinelli ◽  
Marita Vedovelli Cardoso ◽  
Julieta Rodini Engracia De Moraes
Keyword(s):  
Health Risks ◽  
Protective Equipment ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Antimicrobial Susceptibility Profile ◽  
Alkylbenzene Sulfonate ◽  
Before And After ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Objective. The aim of this study was to characterize the bacteria of a veterinary autopsy room, their antimicrobial susceptibility profile and testing the efficiency of two sanitizers against these microorganisms. Materials and methods. Three points of the room that professionals do not normally wear personal protective equipment (PPE) getting in direct contact with these bacterias. Anaerobic and aerobic mesophilic count were performed before and after disinfection with hypochlorite and sodium alkylbenzene sulfonate then, isolates were identified by their morphotintorials and biochemical characteristics and their antibiotic susceptibility. Results. Preliminary results indicated that the hypochlorite was the sanitizing agent of choice for surface disinfection and against the most frequent potential pathogenic bacterials isolated such as Staphylococcus spp (75%), E. coli and Klebsiella spp (15%), and Pseudomonas spp (10%). In addition, 25% of the staphylococci were resistant to at least one antimicrobial tested and Klebsiella spp, E. coli, and Pseudomonas spp were taken into consideration, wide antimicrobial resistance tested were observed. Conclusions. The characterization of these bacteria found in the autopsy room is important to alert professionals about the biological risks they are exposed to, as well as the precautions they should take.

PREVALENCIA DE INFECCIÓN URINARIA, UROPATÓGENOS Y PERFIL DE SUSCEPTIBILIDAD ANTIMICROBIANA, MEDELLÍN 2011-2012

2014 ◽  
Vol 39 (4) ◽  
pp. 352-358
Author(s):  
Jaiberth Antonio Cardona Arias ◽  
Claudia Patricia Orrego Marin ◽  
Claudia Patricia Henao Mejia
Keyword(s):  
Escherichia Coli ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Enterococcus Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Introducción: Las infecciones del tracto urinario (ITU), constituyen una de las infecciones bacterianas más prevalentes, sus agentes etiológicos incluyen Escherichia coli, Klebsiella spp, Proteus spp, Enterococcus spp, Pseudomonas spp y Staphylococcus spp, los cuales presentan prevalencias y perfiles de susceptibilidad antibiótica diferentes entre poblaciones. Objetivo: Determinar la prevalencia de ITU, uropatógenos y el perfil de susceptibilidad antimicrobiana en una Institución Prestadora de Servicios de Salud (IPS) de Medellín, 2011-2012. Métodos: Estudio de prevalencia en 1.959 individuos atendidos en una IPS de tercer nivel. Se calcularon medidas de resumen, proporciones, razones de prevalencia, Chi cuadrado y Fisher. Se cuantificó la modificación del efecto (confusión o interacción) con análisis estratificado y modelos de regresión logística binaria en SPSS 21.0®. Resultados: La prevalencia de ITU fue 31% los principales agentes etiológicos fueron E. coli (69%), Enterococcus spp (11%) y Klebsiella spp (8%). La ITU y la infección por E. coli fueron estadísticamente mayores en mujeres y adultos mayores. La mayor frecuencia de resistencia de E. coli fue para ampicilina (61%), Ácido nalidixico (48%), TrimetropinSulfa (48%) y Ciprofloxacina (42%); mientras que en Klebsiella spp fue Ampicilina (100%), TrimetropinSulfa (23%), Ampicilina- Sulbactam (22%) y Cefalotina (19%). Conclusión: La elevada prevalencia de ITU, la multiplicidad de uropatógenos aislados, la identificación de grupos de mayor riesgo y la diversidad de perfiles de resistencia antibiótica, evidencian la necesidad de desarrollar investigaciones locales que permitan orientar las acciones en salud y vigilancia epidemiológica, acordes con las particularidades de cada población.

scholarly journals Infecções do trato urinário (ITU) de cães e gatos: etiologia e resistência aos antimicrobianos

2014 ◽  
Vol 34 (1) ◽  
pp. 62-70 ◽  
Author(s):  
Vania M. Carvalho ◽  
Tatiana Spinola ◽  
Fabrizia Tavolari ◽  
Kinue Irino ◽  
Rosana M. Oliveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Enterococcus Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

As infecções bacterianas do trato urinário (ITUs) são causa comum de doença em cães, gatos e humanos. Embora bactérias Gram positivas como Staphylococcus spp., Streptococcus spp. e Enterococcus spp., possam ocasionar ITUs, as bactérias Gram negativas (Escherichia coli, Proteus spp., Klebsiella spp., Pseudomonas spp. e Enterobacter spp.) respondem por 75% dos casos. Este estudo teve como objetivo determinar a frequência de diferentes gêneros de bactérias em ITUs em cães e gatos, bem como a sua sensibilidade aos antimicrobianos utilizados na rotina clínica. Portanto, amostras de urina de 100 cães e gatos com sinais de ITU foram coletadas assepticamente, sofrendo avaliação microbiológica por meio de métodos qualitativos e quantitativos, além de urinálise. Todos os isolados foram submetidos a testes de sensibilidade aos antimicrobianos. ITU foi confirmada em 74% dos animais, não havendo predominância quanto ao sexo. No que diz respeito à idade, 85% dos cães e 87% dos gatos tinham idades superiores a seis anos. Noventa e cinco cepas bacterianas foram isoladas, com maior frequência de Escherichia coli (55% do total) dos sorogrupos O6 e O2. Constatou-se níveis elevados de resistência a antimicrobianos nas cepas isoladas. Para as cepas Gram positivas, tetraciclina (46,1%), enrofloxacina, cotrimazol e estreptomicina (42,3% cada) foram as drogas com os maiores índices de resistência. Para as Gram negativas, amoxacilina e tetraciclina apresentaram percentuais acima de 50%. Multiresistência foi verificada em mais de 50% dos principais gêneros isolados. Considerando-se que as cepas de E. coli apresentam potencial zoonótico e forte participação na disseminação de resistência aos antimicrobianos, ressalta-se a importância do papel do médico veterinário na prevenção e controle das ITUs animais e sua contribuição para a saúde pública.

Bakterienisolate aus dem unteren Respirationstrakt von erkrankten Hunden und deren aktuelle Resistenzsituation

2012 ◽  
Vol 40 (05) ◽  
pp. 309-317 ◽  
Author(s):  
E. Prenger-Berninghoff ◽  
N. Bauer ◽  
R. Weiß ◽  
A. Moritz ◽  
A. Steinfeld
Keyword(s):  
Escherichia Coli ◽  
Polymyxin B ◽  
Bordetella Bronchiseptica ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Klinische Relevanz ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Zusammenfassung Gegenstand: Retrospektive Untersuchung der mittels bronchoalveolärer Lavage (BAL) gewonnenen Keimflora der tiefen Atemwege erkrankter Hunde auf ihre Antibiotikaempfindlichkeit über 5 Jahre. Material und Methoden: Auswertung der Ergebnisse der Agardiffusionstests von Bakterienisolaten, die in den Jahren 2004–2009 von 84 Hunden isoliert wurden, und Vergleich mit den Resultaten einer gleichartigen Analyse der Jahre 1999/2000. Ergebnisse: Bei den 99 geprüften Bakterienisolaten handelte es sich um Pasteurella spp. (27,3%), Bordetella bronchiseptica (20,2%), Staphylococcus spp. (18,2%), Escherichia coli (15,2%), Klebsiella spp. (8,1%), Pseudomonas spp. (7,0%) und Streptococcus spp. (4,0%). Bei acht Hunden lagen Mischkulturen vor. Die Mehrzahl der Bordetella-(B.-)bronchiseptica-Isolate erwies sich als sensibel gegenüber den Fluorchinolonen sowie Tetracyclin, Doxycyclin und Polymyxin B. Im Vergleich zu 1999/2000 zeigte sich ein höherer Anteil der gegenüber Amoxicillin/Clavulansäure und Chloramphenicol empfindlichen B.-bronchiseptica-Isolate, wobei die Zunahme bezüglich Chloramphenicol statistisch signifikant war. Die überwiegende Anzahl der Staphylococcus-Isolate erwies sich gegenüber Enrofloxacin, Marbofloxacin, Amoxicillin/Clavulansäure, Chloramphenicol, Cephalexin, Doxycyclin und Polymyxin B sensibel. Im Vergleich zu 1999/2000 ergab sich ein erhöhter Anteil an Chloramphenicolund Tetracyclin-sensiblen Staphylococcus-Isolaten. Der Anteil Enrofloxacin-sensibler KlebsiellenIsolate betrug 62,5%, während er 1999/2000 bei 100% lag. Eine Sensibilität aller getesteten Klebsiellen-Isolate ließ sich noch gegenüber Polymyxin B nachweisen. Kein Antibiotikum zeigte eine Wirksamkeit gegenüber allen E.-coli-Isolaten. Statistisch signifikant war die Abnahme der gegenüber Amoxicillin/Clavulansäure-sensiblen Isolate. Schlussfolgerung und klinische Relevanz: Die Studie unterstreicht den Nutzen der BAL für eine ätiologische Diagnostik und den anschließenden fundierten Einsatz von Antibiotika. Die Mehrzahl der Bakterienisolate erwies sich als empfindlich gegenüber den Fluorchinolonen.

scholarly journals Antibiotic Susceptibility Profile of Bacteria Isolated from Fitness Machines in Selected Fitness Centers at Akure and Elizade University in Ondo State Nigeria

2019 ◽  
pp. 1-9
Author(s):  
O. S. Fadare ◽  
O. B. Durojaye
Keyword(s):  
Antibiotic Susceptibility ◽  
Pathogenic Bacteria ◽  
Physiological Solution ◽  
Susceptibility Pattern ◽  
E Coli ◽  
Before And After ◽  
Bacillus Spp ◽  
Microbiological Techniques ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Aim: This study seeks to determine the antibiotic susceptibility pattern of bacteria isolated from surfaces of fitness machines at fitness center located at Elizade University and Akure town. Methods: Samples were collected from the different site of gym equipment including thread mill (handle, floor), bicep bench (handle), bike (handle, paddle), cruncher (handle, elbow) using sterile swab stick moistened with sterile buffered physiological solution. The swab sticks were immediately transferred to the laboratory for analysis. Standard microbiological techniques were used to identify the bacterial isolates. The antibiotic susceptibility profile of the isolates was determined by using standard antibiotics discs.  Results: Out of the 31 isolates identified, Staphylococcus aureus 12(38.7%) was the predominant bacteria followed by Bacillus spp. 11(35.5%), Klebsiella spp. 4(12.9%), E. coli and Staphylococcus saprophyticus 2(6.45%) and Enterococcus spp. 1(3.23%). The susceptibility profile showed that all isolates were resistant to Amoxicillin (AM) and Augmentin (AU), Staphylococcus spp. isolated from different surfaces shows different susceptibility pattern to the used antibiotics, while Bacillus spp. Klebsiella spp. and E. coli also confer resistance to more than one commonly used antibiotic. Conclusion: The results showed the occurrence of potential pathogenic bacteria in which their presence on the equipment surfaces could easily be transmitted between users and to the environment generally. The spread of these potential pathogenic microorganisms in the fitness centre can be prevented through frequent hand washing and use of hand sanitizer as well as daily cleaning of equipment surfaces before and after activities with disinfectants.

scholarly journals Estimation of microbiological propagation and antimicrobial traits of the frequently accessible flowers

2015 ◽  
Vol 4 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Marufa Sharmin ◽  
Kamal Kanta Das ◽  
Mrityunjoy Acharjee
Keyword(s):  
Pathogenic Bacteria ◽  
Antimicrobial Activities ◽  
Salmonella Spp ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Bacillus Spp ◽  
Almost All ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Present study portrayed a complete microbiological profile of commonly available flowers including Rosa kordesii, Gladiolus hybrid, Acmella oleracea, Nyctanthes arbortristis and Pseudomussaenda flava which were randomly collected from Dhaka city, Bangladesh. The microbial contamination was quantified up to 108 cfu/g. Exploration of specific pathogenic bacteria was estimated within the range of 103 to 108 cfu/g of which Pseudomonas spp. was found in G. hybrid, A. oleracea and P. flava (~106 cfu/g), whereas Escherichia coli and Staphylococcal contamination was evident in almost all samples up to 108 cfu/g. The in vitro antimicrobial activities of the flower extracts were notable against most of the test bacteria. The ethanolic extracts of R. kordesii showed anti-bacterial activity against most of the bacteria except E. coli and Salmonella spp. G. hybrid extracts showed activity against Klebsiella spp. and Bacillus spp., A. oleracea against E. coli, Pseudomonas spp., Bacillus spp., Klebsiella spp., Staphylococcus spp. and Salmonella spp., P. flava against Pseudomonas spp. and Bacillus spp., and N. arbortristis against Bacillus spp. The methanol extracts of G. hybrid possessed activity against E. coli, Listeria spp. and Pseudomonas spp., N. arbortristis extracts against E. coli, Vibrio spp., Bacillus spp., Klebsiella spp. and Staphylococcus spp., P. flava extracts against E. coli. DOI: http://dx.doi.org/10.3329/sjm.v4i1.22756 Stamford Journal of Microbiology, Vol.4(1) 2014: 19-23

scholarly journals Molecular Characterization of Isolated Multidrug-Resistant Bacteria from Tertiary Care Hospitals of Ahmedabad: A Comparison Study Between Previous to COVID-19 and Current Scenario

2021 ◽  
Author(s):  
Anurag D. Zaveri ◽  
Dilip N. Zaveri ◽  
Lakshmi Bhaskaran
Keyword(s):  
Molecular Characterization ◽  
Tertiary Care ◽  
Future Generation ◽  
Multidrug Resistant ◽  
Resistant Bacteria ◽  
E Coli ◽  
Pseudomonas Spp ◽  
Bacillus Spp ◽  
Current Scenario ◽  
Klebsiella Spp

Hospital Acquired Infections (HAIs) are a significant concern for healthcare setups, as it increases the overall cost of treatment, patients stay in hospitals, making them susceptible to secondary and tertiary infections and, sometimes, mortality1. To prevent or control HAIs, evaluating the organisms isolated from the critically maintained areas is considered of epitome importance and everlasting practice in the healthcare industry. Identifying such organisms and screening them for antibiotic resistance is mandatory, but it also helps professionals understand colonization trends. Sensitive areas of healthcare setups were screened monthly from years 2017 to 2020. A total of 4400 samples of hospital hygiene, e.g., intravenous drip stands, ventilator surface, anesthetist’s trolley, patient’s bed, instrument trolley, etcetera, were collected. Isolated organisms were cultured and screened using the CLSI technique. E. coli, Pseudomonas spp., and Klebsiella spp. were found in both previous to COVID current samples. Multidrug-resistant organisms were subjected to molecular characterization to detect the presence of carbapenem genes. Evaluation data of both pre-and during Coronavirus Disease or COVID-19 were compared. The prevalence of pathogenic (Klebsiella spp., E. coli, and Pseudomonas spp.) and non-pathogenic (Staphylococcus aureus and Bacillus spp.) strains in healthcare setups decreased drastically (Klebsiella spp. from 80% to 20%, E.coli from 90% to 10% and Pseudomonas spp. from 80% to 20%). It is possible only because of the awareness in non-specialists and healthcare workers due to the unforeseen critical situation proving to be a blessing for the future generation.

scholarly journals IN INTRA-ABDOMINAL INFECTIONS AND PERITONITIS

2021 ◽  
pp. 90-96
Author(s):  
A. V. Kotov ◽  
V. I. Desyaterik ◽  
S. P. Mikhno
Keyword(s):  
Absolute Number ◽  
Multidrug Resistant ◽  
E Coli ◽  
The Past ◽  
Rational Therapy ◽  
Acinetobacter Spp ◽  
The Difference ◽  
Abdominal Infections ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Summary. Purpose of the reserche. To study changes in microflora and sensitivity of isolated strains to antibiotics using bacteriological research methods for intra-abdominal infections and peritonitis to determine their resistance when planning antibiotic therapy. Results and discussion. The paper presents data from a retrospective analysis of the results of studies of microflora and its resistance in intra-abdominal infections (IAI) and peritonitis for 2 periods: I period — from january 2007 to december 2008, II period — from january 2018 to december 2019. The difference between period II and I was in the increase in the number of individual pathogenic strains of microorganisms. Thus, the number of Klebsiella spp. increased from 13.8 % to 23.8 %, which in absolute number of their total number amounted to a 73.5 %, ranking second in the overall structure among pathogens sown in IAI and peritonitis. The number of strains of Staphylococcus spp., Enterobacter spp., Acinetobacter spp. has also increased by 2.4–5.8 % respectively. Among the strains Klebsiella spp., E. coli and Staphylococcus spp. the number of poly- and multi-polyresistant forms has increased. Thus, the number of such forms among Klebsiella spp. increased from 19.2 to 44.5 %, Staphylococcus spp. — from 43.8 to 67.1 % and E. coli from 18.2 to 23.5 %, respectively. Conclusions. Over the past 10 years, with IAI and peritonitis, the most significant changes were revealed on the part of pathogenic strains of Klebsiella spp., Staphylococcus spp. and Acinetobacter spp. Multidrug resistant strains were predominantly susceptible to carbapenems, semisynthetic ureidopenicillins, aminoglycosides, glycopeptides, oxazalidinones, phosphonoic acid derivatives. In the context of changes in the microbial landscape observed over the past decade, an increase in the number of poly- and multi-resistant forms of pathogenic microorganisms in IAI and peritonitis, the choice of antibiotics for rational therapy should be based solely on bacterioscopy and antibiograms.

scholarly journals Isolation and characterization of bacteria residing in the oral, gut, and fecal samples of different pheasant species

2023 ◽  
Vol 83 ◽  
Author(s):  
M. Mushtaq ◽  
S. M. Bukhari ◽  
S. Ahmad ◽  
A. Khattak ◽  
M. B. Chattha ◽  
...  
Keyword(s):  
Bacterial Species ◽  
Gut Contents ◽  
Phasianus Colchicus ◽  
Gut Content ◽  
E Coli ◽  
Colony Forming Unit ◽  
Isolation And Characterization ◽  
Staphylococcus Spp ◽  
Campylobacter Spp

Abstract There is a paucity of research conducted on microbial prevalence in pheasants. The microbiota of captive birds has zoonotic significance and must be characterize. Present study is therefore planned to assess the microbiota from oral, fecal and gut content of captive avian species. It will be helpful in characterization of harmful microbes. Different samples taken from oral, gut and feces of ring-necked pheasants (Phasianus colchicus), green pheasants (Phasianus versicolor), golden pheasant (Chrysolophus pictus) and silver pheasant (Lophura nycthemera). Samples were collected, diluted, and inoculated onto different agar plates (MacConkey, SS agar, MSA and nutrient agar) for cultivation of bacterial species. Colonies of E.coli, Staphylococcus spp. Brachyspira spp. and Campylobacter spp were observed based on colony morphology. Colony forming unit showed E. coli as frequently found bacteria in fecal, oral and gut contents of all the above pheasants. The overall significance difference was found among bacterial species of golden pheasants, green pheasant, ring-necked pheasant, and silver pheasants. It was concluded that E.coli is predominant isolated from heathy pheasants followed by Campylobacter, Staphylococcus and Brachyspira.

scholarly journals Prevalence of bovine mastitis pathogens and antibioresistance of Escherichia coli isolates

2017 ◽  
Vol 55 (2) ◽  
pp. 113
Author(s):  
A. ZDRAGAS (Α. ΖΔΡΑΓΚΑΣ) ◽  
P. TSAKOS (Π. ΤΣΑΚΟΣ) ◽  
K. ANATOLIOTIS (Κ. ΑΝΑΤΟΛΙΩΤΗΣ)
Keyword(s):  
Escherichia Coli ◽  
Bovine Mastitis ◽  
Clinical Mastitis ◽  
Resistance Rate ◽  
Northern Greece ◽  
E Coli ◽  
Milk Samples ◽  
Staphylococcus Spp ◽  
Klebsiella Spp ◽  
Mastitis Pathogens

Nine hundred and fifty two milk samples from clinical bovine mastitis cases, originated from 269 farms in Northern Greece, were tested. Escherichia coli was isolated in 49.3% of samples. Furthermore, Staphylococcus spp, Corynebacterium spp, Streptococcus spp, Pseudomonas spp, Proteus spp, Klebsiella spp or a combination of the above bacteria were isolated. The bacteriological result from 4.3% of samples was negative. No correlation between clinical mastitis cases and seasonal variation was observed. Resistance of E. coli isolates to tetracycline was 82-96%, to enrofloxacin 10-30%, to gentamicin 58-80%, to cephalosporins 47-75%, to sulfamethoxazole-trimethoprime 46-81%, to ampicillin 71-92% and to neomycin 83-97%. The highest resistance rate and the appearance of multi-resistant isolates of E. coli (6%), to 8 antibacterials, were recorded during the last year of the survey.

scholarly journals Análisis epidemiológico de las infecciones urinarias en pacientes ambulatorios del Centro Médico Bautista

2020 ◽  
Vol 8 ◽  
pp. 109
Author(s):  
Lisa Ibarrola de Ávila ◽  
Lourdes Talavera ◽  
María Marta González ◽  
Fernando Ávila
Keyword(s):  
Escherichia Coli ◽  
Pseudomonas Spp ◽  
Enterococcus Spp ◽  
Las Mujeres ◽  
Staphylococcus Spp ◽  
Los Grupos ◽  
Klebsiella Spp

Las infecciones del tracto urinario son las infecciones más comunes en todo el mundo y se presentan en todos los grupos de población, se estima que entre el 40% y el 50% de las mujeres  presentarán al menos una durante su vida, y de estas el  11% tendrá una infección al año, siendo la causa más frecuente de origen bacteriano (80%-90%). Constituyen una causa frecuente de consulta en atención primaria, afecta mayoritariamente a las mujeres, generalmente son monobacterianas ,sus  agentes etiológicos incluyen :  Escherichia coli, Klebsiella spp, Proteus spp, Enterococcus spp, Pseudomonas spp y Staphylococcus spp, los cuales presentan prevalencias y perfiles de susceptibilidad antibiótica diferentes entre poblaciones por lo que es relevante contar con esta  información para generar un perfil clínico-epidemiológico propio que permita la implementación de medidas terapéuticas correctas, así como  la atención y control de la resistencia bacteriana, acordes con las particularidades de la población del lugar de estudio.

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