Photobioreactor Stability by Phase Plane Technique Applied to Spirulina Maxima Growth
Spirulina maxima is a worthy multicellular filamentous micro-algae used as a food supplement and raw material for fine chemicals and biologically active compounds production. Intensive approach consisting of cultivating pure strains of this photoautotroph microorganism in photobioreactors is more desirable than extensive ones, largely incontrollable with regard to production stability. Determining the best reaction conditions to reach a steady state in the runway events is often needed in biological systems. For a biochemical engineer, knowing the system stability for an optimal bioreactor configuration is crucial to estimate the rate at which dependent variables grow or decay with the time reaction. The stability analysis becomes important in recycle processes in which possibility that these systems influence themselves exists. The aim of this work deals with the 1) description of the growth kinetics by a logistic and unstructured model based on Monod kinetics taking into account the maintenance in life of viable cells; 2) establishment of a dynamic growth model for Spirulina maxima cultivated in continuous lamellar photobioreactors using industrial manures as macro-nutrients; 3) determination of optimal culture conditions sustaining a stable growth of S. maxima in a system of two bioreactors in series; and 4) investigation of the dynamic stability of this multivariable system with nonlinear dynamics using phase plane technique (PPT). Although good mixing of the culture is essential for ensuring adequate supply of nutrients and prevention of the accumulation of toxic metabolites. Excessive agitation causes mechanical damage to Spirulina cells. An air flow rate of 2.5 L/min for airlift agitation represented a balance between the need to provide good mixing and to avoid cell damage. A stable steady state was achieved corresponding to a productivity of 10.8 g. m2/day when the system was supplied with 0.2 g N/L of minerals, at a dilution rate of 0.1 1/day, temperature of 30 °C under light intensity of 18 Klux. PPT as a powerful procedure successfully predicts the stability of such a complex system very well.