Optimization of an Enzyme Assisted Process for Juice Extraction and Clarification from Litchis (Litchi Chinensis Sonn.)

2007 ◽  
Vol 3 (3) ◽  
Author(s):  
Narendra Shah
Keyword(s):  
Apparent Viscosity ◽  
Desirability Function ◽  
Hydrolytic Enzymes ◽  
Enzymatic Treatment ◽  
Soluble Solids ◽  
Enzyme Treatment ◽  
Optimum Process ◽  
Process Conditions ◽  
Juice Yield ◽  
Juice Extraction

Litchi pulp was treated with various concentration levels of hydrolytic enzymes viz. pectinase (0-0.133%w/w), cellulase (0-0.266%w/w) and hemicellulase (0-0.20%w/w) for different durations (30-150 min) at 45°C. The effect of enzyme treatment conditions was studied on yield, clarity, apparent viscosity and total soluble solids (TSS) of juice obtained from the pulp. The optimum process conditions were determined by employing a second order central composite rotatable design in combination with response surface methodology. Yield, clarity and TSS of juice were found to increase and apparent viscosity was found to decrease significantly by enzymatic treatment. The optimum conditions for enzymatic treatment of pulp obtained after a 2 sided desirability function with the responses juice yield, clarity and TSS to be maximized and viscosity to be minimized were 0.076% (w/w) pectinase, 0.138% (w/w) cellulase, 0.107% (w/w) hemicellulase and incubation time of 106.5 min. The predicted values for juice yield, clarity, viscosity and TSS under optimized conditions were 77.19 %, 93.53%, 1.359mPa s and 19.68°brix which showed a good agreement with the experimental values under the same set of conditions.

Optimization of an Enzyme Assisted Process for Juice Extraction and Clarification from Litchis (Litchi Chinensis Sonn.)

2007 ◽  
Vol 3 (2) ◽  
Author(s):  
Narendra Singh Shah ◽  
Nirankar Nath
Keyword(s):  
Apparent Viscosity ◽  
Desirability Function ◽  
Hydrolytic Enzymes ◽  
Enzymatic Treatment ◽  
Soluble Solids ◽  
Enzyme Treatment ◽  
Optimum Process ◽  
Process Conditions ◽  
Juice Yield ◽  
Juice Extraction

Litchi pulp was treated with various concentration levels of hydrolytic enzymes viz. pectinase (0-0.133%w/w), cellulase (0-0.266%w/w) and hemicellulase (0-0.20%w/w) for different durations (30-150 min) at 45°C. The effect of enzyme treatment conditions was studied on yield, clarity, apparent viscosity and total soluble solids (TSS) of juice obtained from the pulp. The optimum process conditions were determined by employing a second order central composite rotatable design in combination with response surface methodology. Yield, clarity and TSS of juice were found to increase and apparent viscosity was found to decrease significantly by enzymatic treatment. The optimum conditions for enzymatic treatment of pulp obtained after a 2 sided desirability function with the responses juice yield, clarity and TSS to be maximized and viscosity to be minimized were 0.076% (w/w) pectinase, 0.138% (w/w) cellulase, 0.107% (w/w) hemicellulase and incubation time of 106.5 min. The predicted values for juice yield, clarity, viscosity and TSS under optimized conditions were 77.19%, 93.53%, 1.359mPa s and 19.68°brix which showed a good agreement with the experimental values under same set of conditions.

scholarly journals Evaluation of Process Conditions for Ultrasonic Spray-Freeze Drying of Transglutaminase

2020 ◽  
Vol 58 (1) ◽  
pp. 38-48
Author(s):  
Hilal Isleroglu ◽  
Izzet Turker
Keyword(s):  
Enzyme Activity ◽  
Flow Rate ◽  
Freeze Drying ◽  
Desirability Function ◽  
Spherical Particles ◽  
Optimum Process ◽  
Process Conditions ◽  
Plate Temperature ◽  
Ultrasonic Spray ◽  
Spray Freeze Drying

In this study, a commercial transglutaminase enzyme was dried using an ultrasonic spray freeze drying method and the effects of the process conditions were optimized to maximize the final transglutaminase activity. Accordingly, process parameters affecting enzyme activity were selected, such as nozzle frequency (48 and 120 kHz), flow rate (2, 5 and 8 mL/min) and plate temperature for secondary drying (25, 35 and 45 °C). Moreover, the effects of different pH values (pH=2.0 and 9.0) and high temperature (80 °C) on enzyme activity, physical properties and particle morphology of transglutaminase were discussed. According to the results, transglutaminase preserved its activity despite ultrasonic spray freeze drying. Sonication enhanced the enzyme activity. Using the desirability function method, the optimum process conditions were determined to be flow rate 3.10 mL/min, plate temperature 45 °C and nozzle frequency 120 kHz. The predicted activity ratio was 1.17, and experimentally obtained ratio was 1.14±0.02. Furthermore, enzyme produced by ultrasonic spray freeze drying had low moisture values (2.92-4.36 %) at 8 h of drying. When the morphological structure of the transglutaminase particles produced by ultrasonic spray freeze drying under the optimum conditions was examined, spherical particles with pores on their surfaces were observed. In addition, flow properties of the transglutaminase powders were considered as fair under most conditions according to the Carr index.

scholarly journals Effect of Multi-enzyme (Pectinase, Cellulase and Hemicellulase) Treatment on Clarification of Papaya (Carica papaya) Fruit Juice

2019 ◽  
Vol 8 (4) ◽  
pp. 12708-12721
Keyword(s):  
Incubation Time ◽  
Fruit Juice ◽  
Incubation Temperature ◽  
Enzyme Concentration ◽  
Enzyme Treatment ◽  
High Yield ◽  
Optimum Process ◽  
Process Conditions ◽  
Simultaneous Treatment ◽  
Market Opportunities

Clarified fruit juices are preferred by a significant portion of the population and are becoming popular in global fruit juice market. Many of the other processed fruit products like, clear juice cocktails, cordials, ready to serve drinks, clear nectars, clear juice blends, translucent jelly products, candies etc. are made from clarified juices. Apart from the traditional clear juices from apple and other citrus fruits, several market opportunities exist for clarified juices produced from the tropical fruits of high pulp content like banana, papaya, guava, mango etc. which are being produced abundantly in India. This study was carried out with an objective of producing high quality clarified papaya juice with high yield by multi-enzyme treatment. Papaya juice was given treatments with various concentration levels of the enzymes pectinase, cellulase and hemicellulase for different incubation times (30-150 min) at incubation temperature of 55 0C. The effect of enzyme treatments was studied on turbidity and yield of clarified papaya juice obtained for each enzyme separately, and optimum process conditions were determined. Based on these results papaya juice was given simultaneous treatment of all the three enzymes with compromised process conditions (Pectinase 0.90%, Cellulase 0.40%, and Hemicellulase 0.50% with 90 min incubation time at 55 0C) which produced clarified juice with very small value of turbidity as 11 NTU and yield as 77.4%. RSM(Response Surface Methodology) employing a second order central composite design was used to obtain optimum process conditions for simultaneous treatment with the range of variables for enzymatic treatment process conditions (Enzyme concentration: 0.20-1.20% w/w, 0.20-0.80% w/w and 0.20-0.80% w/w for pectinase, cellulase and hemicellulase respectively, Incubation time 30-150 min and Incubation temperature 55 0C) based on the above experiments. The results obtained for optimum process conditions were very close to that obtained in previous experiments giving the optimum values as 0.87%, 0.50% and 0.50% enzyme concentration for Pectinase, Cellulase and Hemicellulase respectively, and incubation period of 86 min at incubation temperature of 55 0C. Under this condition, the papaya juice was obtained with turbidity value as 9 NTU and 78.8 per cent of yield.

scholarly journals Optimising Tropical Fruit Juice Quality Using Thermosonication-Assisted Extraction via Blocked Face-Centered Composite Design

Processes ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 3
Author(s):  
Norazlin Abdullah ◽  
Nyuk Ling Chin
Keyword(s):  
Ascorbic Acid ◽  
Fruit Juice ◽  
Ascorbic Acid Content ◽  
Soluble Solids ◽  
Quadratic Model ◽  
Juice Quality ◽  
Tropical Fruit ◽  
Total Soluble Solids ◽  
Juice Yield ◽  
Solids Content

Extraction of tropical fruit juice using simple, efficient, and environmentally friendly technologies is gaining importance to produce high quality juices. Juice from pink-fleshed guava, pink-fleshed pomelo, and soursop was extracted using direct and indirect thermosonication methods by varying intensity, time, and temperature, and compared to those extracted using water bath incubation. Improvised models of juice yield, ascorbic acid, and total soluble solids responses were generated by eliminating insignificant model terms of the factors in full quadratic model using backward eliminating procedure. Main effects, 3D, or 4D plots for each response were developed based on factors that influenced the response. Results showed that the best extraction method for guava and pomelo juices were within indirect thermosonication method of 1 kW, 55 °C and 30 min, and 2.5 kW, 54 °C and 23 min, respectively. Direct thermosonication method at 10% amplitude, 55 °C for 2 to 10 min was more suitable for soursop juice. Thermosonicated extraction of tropical fruit juice can improve its juice yield, ascorbic acid content, and total soluble solids content.

Analyzing the Effect of Fiber, Yarn and Fabric Variables on Bagging Behavior of Single Jersey Weft Knitted Fabrics

2013 ◽  
Vol 8 (3) ◽  
pp. 155892501300800 ◽  
Author(s):  
Mitra Karimian ◽  
Hossein Hasani ◽  
Saeed Ajeli
Keyword(s):  
Optimum Process ◽  
Process Conditions ◽  
Knitted Fabrics ◽  
Yarn Twist ◽  
Fabric Density ◽  
Fabric Structure ◽  
Controllable Factors ◽  
Yarn Count ◽  
The Individual ◽  
Single Jersey

This research investigates the effect of fiber, yarn and fabric variables on the bagging behavior of single jersey weft knitted fabrics interpreted in terms of bagging fatigue percentage. In order to estimate the optimum process conditions and to examine the individual effects of each controllable factor on a particular response, Taguchi's experimental design was used. The controllable factors considered in this research are blending ratio, yarn twist and count, fabric structure and fabric density. The findings show that fabric structure has the largest effect on the fabric bagging. Factor yarn twist is second and is followed by fabric density, blend ratio and yarn count. The optimum conditions to achieve the least bagging fatigue ratio were determined.

Study on the Enzyme Desizing Process for Cotton Fabric Using Ultrasonication

2011 ◽  
Vol 331 ◽  
pp. 261-264 ◽  
Author(s):  
Qi Ming Zhao ◽  
Shan Yan Zhang
Keyword(s):  
Cotton Fabric ◽  
Ph Value ◽  
Orthogonal Experiment ◽  
Research Result ◽  
Strength Loss ◽  
Cotton Fabrics ◽  
Optimum Process ◽  
Process Conditions ◽  
Fabric Strength ◽  
Enzyme Dosage

The auxiliary devices of ultrasonic treatment was designed and manufactured. The cotton fabric was desized using 2000L desizing enzyme with the conventional enzyme desizing process and ultrasonic enzyme desizing process respectively. Through the orthogonal experiment, the optimum process conditions of conventional enzyme desizing process and ultrasonic enzyme desizing process were determined. For the conventional enzyme desizing process, the optimized desizing conditions of cotton fabrics were: desizing enzyme dosage was 1.5g/l, temperature was 80°C, PH value was 6, and time was 60mins. The optimum process conditions of ultrasonic enzyme desizing process were: desizing enzyme dosage was 1.5g/l, temperature was 50°C, PH value was 6 and time was 45minutes. The research result indicates that, under the same desizing condition, ultrasonication can improve the desizing percentage and whiteness of cotton fabric, but the fabric strength loss increases slightly. And for the same required desizing percentage, the ultrasonic enzyme desizing process saved time and reduced the temperature of experiments compared with traditional enzyme desizing process

scholarly journals Characterization and Prebiotic Potential of Longan Juice Obtained by Enzymatic Conversion of Constituent Sucrose into Fructo-Oligosaccharides

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2596 ◽  
Author(s):  
Yongxia Cheng ◽  
Haibo Lan ◽  
Lei Zhao ◽  
Kai Wang ◽  
Zhuoyan Hu
Keyword(s):  
Soluble Sugar ◽  
Monosaccharide Composition ◽  
Water Soluble ◽  
Lactobacillus Delbrueckii ◽  
Soluble Solids ◽  
Enzyme Treatment ◽  
Bacteria Growth ◽  
Before And After ◽  
Soluble Polysaccharide

The prebiotic potential of longan juice obtained by a commercial Viscozyme L for conversion of constituent sucrose to fructo-oligosaccharide was investigated. The physicochemical properties and carbohydrate composition of the longan juice was evaluated before and after enzymatic treatment. The stimulation effects of the treated longan juice on probiotic bacteria growth were also studied in vitro. The results showed that total soluble solids, yield and clarity of longan juice were all significantly improved after enzyme treatment. The water-soluble polysaccharide content, including pectin, was significantly increased. Compared with the natural longan pulp, the enzyme treated juice showed a significant decrease in sucrose content. Substantial fructo-oligosaccharides including 1-kestose and nystose were synthesized after enzyme treatment. The molecular weight distribution and the monosaccharide composition of the water-soluble polysaccharide were significantly changed by enzyme treatment. The treated longan juice and its ethanol-soluble sugar fraction promoted the growth of Streptococus thermophiles, Lactobacillus acidophilus and Lactobacillus delbrueckii, showing a good potential of the treated longan juice for producing functional foods and nutraceuticals.

scholarly journals Heterosis in sweet sorghum

2018 ◽  
Vol 53 (5) ◽  
pp. 593-601 ◽  
Author(s):  
Gabrielle Maria Romeiro Lombardi ◽  
Patricia Cardoso Andrade Navegantes ◽  
Carlos Henrique Pereira ◽  
Jales Mendes Oliveira Fonseca ◽  
Rafael Augusto da Costa Parrella ◽  
...  
Keyword(s):  
Sweet Sorghum ◽  
Accumulation Rate ◽  
Diallel Cross ◽  
Soluble Solids ◽  
Male Sterile ◽  
Fertility Restorer ◽  
Juice Extraction ◽  
Per Se ◽  
Solids Content ◽  
Partial Diallel

Abstract: The objective of this work was to evaluate the potential per se of male-sterile and fertility-restorer lines of sweet sorghum (Sorghum bicolor), as well as to detail the heterosis manifested for some traits directly or indirectly related to ethanol production, accumulation rate, and predictability. Evaluations were performed for 20 genotypes, of which 4 are fertility-restorer lines (R), 3 are male-sterile lines (A), and 12 are experimental hybrids (H) resulting from the partial diallel cross between lines A and R, besides a commercial hybrid CV198 used as a check, in four harvest seasons. The experiments were carried out in the municipalities of Lavras and Sete Lagoas, in the state of Minas Gerais, Brazil. The measured traits were plant height, green mass production, juice extraction, total soluble solids content, and megagrams of Brix per hectare. The male-sterile A1 and the fertility-restorer R1 and R3 lines show the best potential per se, considering all traits and their accumulation rate and predictability over harvest times. Heterosis is significant for all traits. The H11, H13, H14, H21, H22, and H33 hybrids are promising because of their better performance per se and higher heterosis.

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