Provitamin A Carotenoïd Content of Dried Fermented Cassava Flour: The Effect of Palm Oil Addition During Processing

Author(s):  
Inocent Gouado ◽  
Adelaide Demasse Mawamba ◽  
Ruphine Solange Meyimgo Ouambo ◽  
Issa Touridomon Some ◽  
Tchouanguep Mbiapo Félicité

Vitamin A deficiency is a public health problem in developing countries. Cassava is a major staple food for most Africans. Most often, it is processed into dried fermented cassava (commonly called gari) to limit post harvest losses. The white or yellow type of gari can be obtained without or with the addition of red palm oil respectively. However in most African countries, the process is still traditional and the quantity of red palm oil varies from one producer to another. This study aimed to standardize the processing of cassava into gari and determine the carotenoïd content of dried fermented cassava roots cooked with different quantities of red palm oil. Grated samples from 210g of cassava tubers were fermented and fried at 120°C ± 5 for 10 minutes with (yellow gari) and without whole red palm oil (white gari). The quantities of red palm oil used were 2, 3, 4, 5 and 8 ml. The alpha and beta carotene contents of gari (experimental samples) and of yellow gari bought from a local market (commercial samples) were evaluated by HPLC. The organoleptic properties of the gari were assessed using 18 volunteers aged between 20 to 30 years accustomed to gari. From the results, the provitamin A carotenoïd content of gari obtained increased significantly (P< 0.05) with the quantity of oil used and were higher than that of the commercial samples. The alpha carotene ranged from 352.6 ± 5.5 µg/g to 1572.5 ± 15.9 µg/g (for 2 and 8 ml of palm oil respectively) and the beta carotene from 309.7 ± 24.8 µg/g to 1624.3 ± 45.8 µg/g (for 2 and 8 ml of oil respectively). These results suggest that the addition of red palm oil (4 ml/210 g of cassava) during the processing of cassava into yellow gari resulted in good retention of carotenoïd. Gari is a dry product, thus if it is well conserved after processing in dark airtight packages, it could be used as a good tool to reduce vitamin A deficiency.

2001 ◽  
Vol 71 (2) ◽  
pp. 111-116 ◽  
Author(s):  
Brahim Lachili ◽  
Henri Faure ◽  
Josiane Arnaud ◽  
Marie-Jeanne Richard ◽  
Cherifa Benlatreche ◽  
...  

Despite trace elements and vitamins are major public health problems in some African countries, there are few studies reporting micronutrient status in North Africa. Therefore, it could be interesting to evaluate plasma concentrations of vitamin A, E and beta-carotene, along with zinc, copper, selenium erythrocyte glutathione peroxidase and superoxide dismutase in Algeria. Volunteers were randomly recruited in Constantine, Batna and Mila. Vitamins, trace elements and enzymes were measured in the University Hospital of Grenoble. 455 persons were included in the study. Subjects were divided in 3 groups: group I: 15 girls and 12 boys who were 6 to 12.9 years old, group II: 190 women and 192 men 13 to 49.9 years old, group III: 24 women and 24 men 50 to 65 years old. Plasma concentrations of micronutrients and enzymes are close to those commonly observed in Europe, except for vitamin A concentrations. Indeed, retinol levels are 30–35% lower than those reported in European countries, moreover almost 8% of the population showed retinol concentrations less than 1.05 mumol/l. Beta-Carotene levels were also lower than in the French average population. Ten per cent of the population had plasma zinc levels lower than 10.6 mumol/l. Vitamin E, copper and selenium status seems satisfactory in Algeria. Vitamin A is not a public health problem, however a significant percentage of residents exhibits impaired vitamin A levels and may benefit from retinol supplementation. Larger studies are needed, and particularly in children, to detect possible higher prevalence of vitamin A deficiency in poor socio-economical classes and in inner geographic areas.


2012 ◽  
Vol 2 (2) ◽  
pp. 60 ◽  
Author(s):  
Beatrice Nakhauka Ekesa ◽  
Judith Kimiywe ◽  
Inge Van den Bergh ◽  
Guy Blomme ◽  
Claudie Dhuique-Mayer ◽  
...  

<p>Changes in the concentrations and retention levels of total and individual provitamin A carotenoids (pVACs) during ripening and local processing of the four most popular <em>Musa</em> cultivars of Eastern Democratic Republic of Congo were established through HPLC analysis. The predominant pVACs were all <em>trans</em> ?- and ?-carotene, together constituting about 90% of total pVACs content in raw and processed <em>Musa</em> fruit pulp. The proportion of ?- and ?-carotene was not significantly different in the tested East African Highland Bananas (AAA-EAHB) (‘Nshikazi’ and ‘Vulambya’); in the plantains (‘Musilongo’ and ‘Musheba’), proportion of ?-carotene was almost twice that of ?-carotene. An increase in total pVACs was observed during ripening, with highest levels at ripening stage 3 in all four cultivars. Total pVACs values were as high as 1081µg/100gfw in ‘Vulambya’ and 1819µg/100gfw in ‘Musilongo’. Boiling of the AAA-EAHB and AAB-Plantains resulted to retention of between 40-90% and &gt;95% respectively. Plantains deep-fried in fully-refined palm oil and crude red palm oil for 2 minutes did not seem to lose any pVACs, the levels of total pVACs observed after frying were 100% of what was observed when the fruit was raw. Retinol Activity Equivalents (RAE), in boiled products varied between 22.3 and 173 RAEµg/100gfw, whereas deep fried products had &gt;190 RAEµg/100g edible portion. These results show that the tested AAA-EAHBs and the plantains could meet at least 14% and 30 % of Vitamin A recommended dietary intakes respectively. The findings can therefore guide consumer consumption patterns to maximize vitamin A intake for improved health in these regions and also direct researchers in the selection of <em>Musa</em> cultivars to be incorporated within existing farming systems in the fight against vitamin A deficiency (VAD).</p>


1994 ◽  
Vol 15 (2) ◽  
pp. 1-8 ◽  
Author(s):  
Choo Yuen May

Crude palm oil is the richest natural plant source of carotenoids in terms of retinol (provitamin A) equivalent. This article reports on » the carotenoids found in palm oil, its fractions, byproducts, and derivatives from the Elaeis guineensis and E. oleifera palms, including their hybrids and a back-cross, as well as the carotenoids of pressed palm fibres, second-pressed oil, palm leaves, and palm-derived alkyl esters; » two novel procedures for preparing highly concentrated sources of carotenoids (>80,000 ppm), by recovery by palm alkyl esters, and by retention and concentration in deacidified and deodorized red palm oil; » the carotenoid content and profiles of the above sources obtained by high-performance liquid chromatography; and » nutritional effects of palm oil carotenoids and their potential applications for health promotion and disease prevention.


2009 ◽  
Vol 102 (3) ◽  
pp. 342-349 ◽  
Author(s):  
Julie A. Howe ◽  
Bussie Maziya-Dixon ◽  
Sherry A. Tanumihardjo

Efforts to increase β-carotene in cassava have been successful, but the ability of high-β-carotene cassava to prevent vitamin A deficiency has not been determined. Two studies investigated the bioefficacy of provitamin A in cassava and compared the effects of carotenoid content and variety on vitamin A status in vitamin A-depleted Mongolian gerbils (Meriones unguiculatus). Gerbils were fed a vitamin A-free diet 4 weeks prior to treatment. In Expt 1, treatments (ten gerbils per group) included 45 % high-β-carotene cassava, β-carotene and vitamin A supplements (intake matched to high-β-carotene cassava group), and oil control. In Expt 2, gerbils were fed cassava feeds with 1·8 or 4·3 nmol provitamin A/g prepared with two varieties. Gerbils were killed after 4 weeks. For Expt 1, liver vitamin A was higher (P < 0·05) in the vitamin A (1·45 (sd 0·23) μmol/liver), lower in the control (0·43 (sd 0·10) μmol/liver), but did not differ from the β-carotene group (0·77 (sd 0·12) μmol/liver) when compared with the high-β-carotene cassava group (0·69 (sd 0·20) μmol/liver). The bioconversion factor was 3·7 μg β-carotene to 1 μg retinol (2 mol:1 mol), despite 48 % cis-β-carotene [(Z)-β-carotene] composition in cassava. In Expt 2, cassava feed with 4·3 nmol provitamin A/g maintained vitamin A status. No effect of cassava variety was observed. Serum retinol concentrations did not differ. β-Carotene was detected in livers of gerbils receiving cassava and supplements, but the cis-to-trans ratio in liver differed from intake. Biofortified cassava adequately maintained vitamin A status and was as efficacious as β-carotene supplementation in the gerbil model.


Nutrients ◽  
2013 ◽  
Vol 5 (8) ◽  
pp. 3257-3271 ◽  
Author(s):  
Ellie Souganidis ◽  
Arnaud Laillou ◽  
Magali Leyvraz ◽  
Regina Moench-Pfanner

2000 ◽  
Vol 51 (sup1) ◽  
pp. s43-s50 ◽  
Author(s):  
M. E. van Stuijvenberg ◽  
M. Faber ◽  
M. A. Dhansay ◽  
C. J. Lombard ◽  
N. Vorster ◽  
...  

1994 ◽  
Vol 15 (2) ◽  
pp. 1-6 ◽  
Author(s):  
C. Rukmini

Red palm oil (RPO), besides providing calorie density to the diet, is also the richest natural source of β-carotene, a precursor of vitamin A and an antioxidant that destroys singlet oxygen and free radicals. Chemical analysis of the fatty acid composition of RPO indicates that it has 50% saturated, 40% mono-unsaturated, and 10% polyunsaturated fatty acids. RPO contains 550 mg/g of total carotenoids, of which 375 mg/g represent β-carotene. It also contains 1,000 mg/g of tocopherols and tocotrienols. Nutritional values in rats fed 10% RPO in a 10% casein diet were comparable to those fed 10% ground nut oil (GNO) or 10% RBDPO (refined, bleached, deodorized palm oil). Rats fed RPO or RBDPO had significantly lower plasma cholesterol concentrations than those fed GNO. Significant inhibition of micro-somal 3-hydroxy-3-methylglutaryl coenzyme A reductase activity was observed in the RPO and RBDPO groups, indicating reduced synthesis of endogenous cholesterol. Toxicological studies also indicate that RPO is safe for human consumption. Indian school children fed supplementary snacks prepared with RPO for 60 days had significant increases in serum retinol levels as well as an increased liver retinol store, suggesting the ready bioavailability of β-carotene.


2006 ◽  
Vol 12 (1) ◽  
pp. 85-90 ◽  
Author(s):  
M. Sadiq Butt ◽  
J. Rasool ◽  
K. Sharif

Cake rusks were prepared by using different combinations of normal and red palm oil fortified shortening to explore the possibility of red palm oil (RPO) fortified shortening to enhance the vitamin A status in human dietary intake. The replacement of normal shortening with RPO up to 50% level was the most acceptable, however, the use of RPO shortening beyond this limit showed a somewhat oily after taste. Carotenoid contents in flour, normal shortening, RPO shortening and egg were 3.57 ppm, 5.85ppm, 405.39 ppm and 5.13 ppm, respectively. Moisture content increased from 3.11% to 3.22% after 28 days of storage. During baking, loss of carotenoid contents ranged from 12.07% to 14.91%. A minimum carotenoid content of 7.19 ppm were found in the cake rusks (T1) prepared with 100% normal shortening (NS) while a maximum of 105.64 ppm was observed in (T5) 100% RPO in the freshly prepared product. The mean carotenoid content in T3 (50% RPO 50% NS) was 57.80ppm however it ranged from 6.94ppm (T1) to 102.34 ppm in (T5). A gradual decrease (7–10%) in carotenoid contents during 28 days’ storage was observed. There was significant increase (0.04–0.08 mg malonaldehyde/kg) in thiobarbituric acid number during storage. Results regarding sensory attributes reflect that T3 comprising of 50% RPO and 50% NS got the highest score. Furthermore consumer response in the acceptability study also showed that T3 treatment of cake rusk is suitable for commercialisation.


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