SYNTHESIS OF CR(III)-ASPARTATE AND CU(II)-ASPARTATE COMPLEXES AS ANTIDIABETIC COMPOUND

The synthesis of Cr(III)-Aspartate and Cu(II)-Aspartate complexes has been successfully conducted by reacting CrCl3·6H2O and CuCl2·2H2O metals with aspartic acid. Therefore, this study aimed to synthesize Cr(Asp)2Cl2 and Cu(Asp)Cl2 as well as test their antidiabetic effects. The synthesis results of Cr(Asp)2Cl2 and Cu(Asp)Cl2 in the form of light purple and blue solids were 0.3001 g and 0.3095g with a yield of 95.14% and 95.02%, respectively. Furthermore, the antidiabetic test used 27 male mice (Mus musculus) with nine treatments for 21 days. The data obtained were analyzed statistically using analysis of variance, and the antidiabetic activity was expressed in percent glucose lowering. The result showed a decrease in blood glucose levels in mice after alloxan induction, with percent glucose lowering (%GL) values of 74.1874% for Cr(Asp)2Cl2 and 76.1337% for Cu(Asp)Cl2 compounds. Therefore, the Cr(Asp)2Cl2 and Cu(Asp)Cl2 compounds can be used as antidiabetic in mice which are also potentially used as metal-based drugs for the treatment of DM.

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Antidiabetic effects of Cycas edentata aqueous leaf extract on the blood glucose levels of alloxan-induced diabetic imprinting control regions mice (Mus musculus L.)

National Journal of Physiology Pharmacy and Pharmacology ◽

10.5455/njppp.2017.7.0726018082017 ◽

2017 ◽

pp. 1

Author(s):

Elisha Elardo ◽

Abbie Olea ◽

Francesca Cruz ◽

Gloriana Teope ◽

Rodel II

Keyword(s):

Blood Glucose ◽

Mus Musculus ◽

Leaf Extract ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Aqueous Leaf Extract ◽

Antidiabetic Effects ◽

Control Regions

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Antidiabetic activities of 96% ethanol extract of Abelmoschus esculentus on Balb-C mice strain

Farmasains Jurnal Farmasi dan Ilmu Kesehatan ◽

10.22219/farmasains.v4i2.10718 ◽

2020 ◽

Vol 4 (2) ◽

Author(s):

Agnis Pondineka Ria Aditama

Keyword(s):

Antioxidant Activity ◽

Blood Glucose ◽

Ethanol Extract ◽

Negative Control ◽

Abelmoschus Esculentus ◽

Antidiabetic Activity ◽

Male Mice ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Reduce Blood Glucose

Diabetes mellitus (DM) is a progressive metabolic disease that continues to increase every year. Treatment therapy using oral hypoglycemic drugs to treat DM has side effects, and alternative approaches must be taken. Abelmoschus esculentus has been known to be used as an alternative to DM because it has antioxidant activity due to its high flavonoid content. The purpose of this study was to determine the antidiabetic activity of 96% ethanol extract of Okra leaves against male mice of balb-c strain. Male mice were acclimatized for seven days and divided into five test groups and then fasted for 18 hours. Then the mice were induced with 150 mg / 20 g BW alloxan and measured on the 3rd day. Ethanol extract 96% okra leaves were then given in a dose of 5.6 mg / 20g BW; 11.2 mg / 20g BW; 22.4 mg / 20g BW, CMC Na 0.5% as negative control and glibenclamide 0.026 mg / 20g BBs as positive control for 14 days. The results of therapy with 96% ethanol extract of okra leaves can reduce blood glucose levels in mice so that the okra leaves contain flavonoid compounds that have antioxidant activity and can reduce blood glucose levels.

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The effectiveness of addition of noni fruit (Morinda citrifolia L) in yoghurt on blood glucose levels of male mice (Mus musculus)

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/247/1/012009 ◽

2019 ◽

Vol 247 ◽

pp. 012009

Author(s):

Rasbawati ◽

Irmayani

Keyword(s):

Blood Glucose ◽

Mus Musculus ◽

Morinda Citrifolia ◽

Male Mice ◽

Glucose Levels ◽

Blood Glucose Levels

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Antidiabetic Activity Test of Sambiloto Capsules (Andrographis paniculata) with Glibenclamide on White Male Mice DDY Strain

Jurnal Kefarmasian Akfarindo ◽

10.37089/jofar.v0i0.83 ◽

2020 ◽

pp. 19-26

Author(s):

Monik Krisnawati

Keyword(s):

Diabetes Mellitus ◽

Blood Glucose ◽

White Male ◽

Andrographis Paniculata ◽

Antidiabetic Activity ◽

Control Group ◽

Male Mice ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Paired T Test

Diabetes mellitus is a metabolic disorder characterized by hyperglycemia and metabolic abnormality of carbohydrates, fats, and proteins caused by decreased insulin sensitivity. Glibenclamid as a synthesis drug or traditional medicine such as Sambiloto Capsule could use for diabetes mellitus. Hereditary, people have been using decoction of Sambiloto’s leaves to prevent cold, influenza, fevers, jaundice. The bitterness of Sambiloto (Andrographis paniculata) was also believed to cure diabetes. The purpose of this research was to compare between Sambiloto capsule with Glibenclamid on white male mices DDY strain. The research method was an experimental study using pre and post test control group design, using 15 white male mices DDY strain which were given a glucose load and divided into 3 groups. Negatif control group (CMC Na), positive control group (Glibenclamid), and test group (Sambiloto Capsules). The results blood glucose level of this research was analyzed using Paired T-test and with the 95 % convidence level. The comparison test of antidiabetes activity between Sambiloto Capsule and Glibenklamid on white male mice DDY strain showed that the Sambiloto Capsule had antidiabetic activity viewed from the average decline in blood glucose levels. The degradation of blood glucose levels in Glibenklamid group was faster than Sambiloto Capsule group indicated by the statistical analysis using Paired T-test with the significance level smaller than 0,05 and showed by T-value was great than T-tabel. Conclusion of the research showed that the activity Sambiloto Capsules anti diabetic.

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Efek Ekstrak Etanol Kulit Petai (Parkia speciosa Hassk) Terhadap Penurunan Kadar Glukosa Darah Mencit Jantan

Jurnal Katalisator ◽

10.22216/jk.v3i1.2178 ◽

2018 ◽

Vol 3 (1) ◽

pp. 1

Author(s):

Verawaty Verawaty ◽

Dhea Claudia Novel

Keyword(s):

Blood Glucose ◽

Ethanol Extract ◽

Negative Control Group ◽

Positive Control ◽

Negative Control ◽

Control Group ◽

Male Mice ◽

Glucose Levels ◽

The Third ◽

Blood Glucose Levels

Penelitian ini bertujuan untuk melihat pengaruh pemberian ekstrak etanol kulit petai (Parkia speciosa Hassk) terhadap penurunan kadar glukosa darah mencit jantan yang diinduksi aloksan. Hewan percobaan dibagi atas 5 kelompok diantaranya kelompok kontrol negatif, kelompok kontrol positif,dosis I (280 mg/kgBB mencit), dosis II (560 mg/kg BB mencit), dosis III (840 mg/kg BB mencit). Penelitian dilakukan selama 21 hari. Persentase penurunan kadar glukosa darah mencit jantan setelah diberikan ekstrak etanol kulit petai pada hari ke-21 adalah dosis I (77,52 %) lebih besar dibandingkan dengan dosis II (69,5 %) dan dosis III (73,37 %). Data yang diperoleh dianalisis dengan uji Two Way Anova dengan program SPSS 17. Hasil penelitian ini menunjukkan bahwa pemberian ekstrak etanol kulit petai untuk tiga variasi dosis menyatakan perbedaan yang bermakna secara statistik terhadap penurunan kadar glukosa darah mencit jantan.Petai (Parkia speciosa Hassk) has a compound β-sitosterol and stigmasterol that have efficacy to decreased blood glucose levels. This study aimed to determine the effect of ethanol extract of petai peel for decrease blood glucose levels of male mice induced by alloxan. Experimental animals were divided into 5 groups including negative control group, positive control group, the first dose (280 mg/kg in mice), the second dose (560 mg/kg in mice), the third dose (840 mg/kg in mice). The study was conducted for 21 days. After 21 days, the result found that the percentage of blood glucose levels after the male mice given the ethanol extract of petai peel was, the first dose (77.52%) biger than the second dose (69.5%) and the third dose (73.37%). The data obtained were analyzed by Two Way ANOVA using SPSS 17. The results showed that have signicantly difference between three dose variation of ethanol extract of petai peel in blood glucose levels.

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Role of Melanocortin Signaling in Neuroendocrine and Metabolic Actions of Leptin in Male Rats With Uncontrolled Diabetes

Endocrinology ◽

10.1210/en.2014-1169 ◽

2014 ◽

Vol 155 (11) ◽

pp. 4157-4167 ◽

Cited By ~ 16

Author(s):

Thomas H. Meek ◽

Miles E. Matsen ◽

Vincent Damian ◽

Alex Cubelo ◽

Streamson C. Chua ◽

...

Keyword(s):

Male Rats ◽

Melanocortin Receptor ◽

Glucose Lowering ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Uncontrolled Diabetes ◽

Streptozotocin Induced Diabetes ◽

Melanocortin Signaling ◽

Antidiabetic Effects

Abstract Although the antidiabetic effects of leptin require intact neuronal melanocortin signaling in rodents with uncontrolled diabetes (uDM), increased melanocortin signaling is not sufficient to mimic leptin's glucose-lowering effects. The current studies were undertaken to clarify the role of melanocortin signaling in leptin's ability to correct metabolic and neuroendocrine disturbances associated with uDM. To accomplish this, bilateral cannulae were implanted in the lateral ventricle of rats with streptozotocin-induced diabetes, and leptin was coinfused with varying doses of the melanocortin 3/4 receptor (MC3/4R) antagonist, SHU9119. An additional cohort of streptozotocin-induced diabetes rats received intracerebroventricular administration of either the MC3/4R agonist, melanotan-II, or its vehicle. Consistent with previous findings, leptin's glucose-lowering effects were blocked by intracerebroventricular SHU9119. In contrast, leptin-mediated suppression of hyperglucagonemia involves both melanocortin dependent and independent mechanisms, and the degree of glucagon inhibition was associated with reduced plasma ketone body levels. Increased central nervous system melanocortin signaling alone fails to mimic leptin's ability to correct any of the metabolic or neuroendocrine disturbances associated with uDM. Moreover, the inability of increased melanocortin signaling to lower diabetic hyperglycemia does not appear to be secondary to release of the endogenous MC3/4R inverse agonist, Agouti-related peptide (AgRP), because AgRP knockout mice did not show increased susceptibility to the antidiabetic effects of increased MC3/4R signaling. Overall, these data suggest that 1) AgRP is not a major driver of diabetic hyperglycemia, 2) mechanisms independent of melanocortin signaling contribute to leptin's antidiabetic effects, and 3) melanocortin receptor blockade dissociates leptin's glucose-lowering effect from its action on other features of uDM, including reversal of hyperglucagonemia and ketosis, suggesting that brain control of ketosis, but not blood glucose levels, is glucagon dependent.

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Gynura divaricata Effect on Blood Glucose Levels in Alloxan-Induced Diabetes Mice

Proceedings of the Singapore National Academy of Science ◽

10.1142/s2591722619400039 ◽

2019 ◽

Vol 13 (01) ◽

pp. 27-33

Author(s):

Evi Sovia ◽

Dian Anggraeny ◽

Ris Kristiana ◽

Firhan Hamdi Maulida ◽

Mutia Susparini

Keyword(s):

Blood Glucose ◽

Histological Examination ◽

Ethanolic Extract ◽

Ethanol Extract ◽

Antidiabetic Activity ◽

Phytochemical Analysis ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Diabetes Mice ◽

Before And After

Gynura divaricata (GD) also known as Dewa leaf is one of the Indonesian medicinal plants which are also native to India, Thailand and China. GD is widely used as antidiabetic, antihypertensive and other diseases including several tumors. However, research on the effects of GD as antidiabetic is still not widely practiced, especially in Indonesia. The aim of this research is to investigate the effect of GD ethanolic extract on blood glucose levels of alloxan-induced diabetes mice. This research is an experimental research with pre- and post-test design using 24 male DDY strain mice. Animals experiment is divided into four groups, that are one normal group and three alloxan-induced diabetes mice group that give 1% CMC (control), 400[Formula: see text]mg/kg GD ethanolic extract and 0.65[Formula: see text]mg/kg glibenclamide, respectively. Treatment was given for 14 days. Blood glucose levels were measured before and after inducing alloxan, and after 14 days treatment. At the end of study, all of animals experiment were sacrificed for histological examination. Phytochemical analysis revealed that polyphenols, flavonoids, steroids, triterpenoids, terpenoids and and quinones were present in ethanol extract of GD. The results of this study showed significant ([Formula: see text]) decrease of blood glucose levels after GD ethanolic extract and glibenclamide treatment. The results of histological examination showed improvement in pancreas damage and significant increase in the number of beta cells in GD and glibenclamide groups. The results indicate that dewa leaf ethanolic extract has antidiabetic activity and the effectiveness is the same as glibenclamide. GD also could repair damage of pancreas caused by alloxan induction.

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Taxithelium napalense acts against free radicals and diabetes mellitus

Bangladesh Journal of Pharmacology ◽

10.3329/bjp.v12i2.31764 ◽

2017 ◽

Vol 12 (2) ◽

pp. 16 ◽

Cited By ~ 6

Author(s):

Vinay Bharadwaj Tatipamula ◽

Kishore Naidu Killari ◽

Vedula Girija Sastry ◽

Alekhya Ketha

Keyword(s):

Free Radicals ◽

Blood Glucose ◽

Video Clip ◽

Radical Scavenging ◽

Ethanolic Extract ◽

Antidiabetic Activity ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Radical Scavenging Properties ◽

Histopathological Studies

In this work, Taxithelium nepalense ethanolic extract and its fractions were evaluated for the antidiabetic activity in rat based on phytochemical and free radical scavenging properties. The ethanolic extract, fraction IV and V significantly attenuated the blood glucose levels at 600, 200 and 200 mg/kg with 50.0, 33.5 and 42.0% inhibition respectively. The histopathological studies were manifesting the recuperation of damaged cells in liver and pancreas tissues. The outcomes of the present work affirm that the T. nepalense has a potency to plummet the overproduction of free radicals and blood glucose levels in the diabetic-induced rat.Video Clip of Methodology:1 min 50 sec <a href="https://youtube.com/v/GUZp4QY9kCs">Full Screen</a> <a href="https://youtube.com/watch?v=GUZp4QY9kCs">Alternate</a>

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Leptin contributes to the beneficial effects of insulin treatment in streptozotocin-diabetic male mice

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.00159.2018 ◽

2018 ◽

Vol 315 (6) ◽

pp. E1264-E1273

Author(s):

Ursula H. Neumann ◽

Michelle M. Kwon ◽

Robert K. Baker ◽

Timothy J. Kieffer

Keyword(s):

Blood Glucose ◽

Insulin Therapy ◽

Daily Injection ◽

Diabetic Mice ◽

Glucose Lowering ◽

Beneficial Effects ◽

Glucose Levels ◽

Lowering Effect ◽

Blood Glucose Levels ◽

Glucose Lowering Effect

It was long thought that the only hormone capable of reversing the catabolic consequences of diabetes was insulin. However, various studies have demonstrated that the adipocyte-derived hormone leptin can robustly lower blood glucose levels in rodent models of insulin-deficient diabetes. In addition, it has been suggested that some of the metabolic manifestations of insulin-deficient diabetes are due to hypoleptinemia as opposed to hypoinsulinemia. Because insulin therapy increases leptin levels, we sought to investigate the contribution of leptin to the beneficial effects of insulin therapy. To do this, we tested insulin therapy in streptozotocin (STZ) diabetic mice that were either on an ob/ ob background or that were given a leptin antagonist to determine if blocking leptin action would blunt the glucose-lowering effects of insulin therapy. We found that STZ diabetic ob/ ob mice have a diminished blood glucose-lowering effect in response to insulin therapy compared with STZ diabetic controls and exhibited more severe weight loss post-STZ injection. In addition, STZ diabetic mice administered a leptin antagonist through daily injection or plasmid expression respond less robustly to insulin therapy as assessed by both fasting blood glucose levels and blood glucose levels during an oral glucose tolerance test. However, leptin antagonism did not prevent the insulin-induced reduction in β-hydroxybutyrate and triglyceride levels. Therefore, we conclude that elevated leptin levels can contribute to the glucose-lowering effect of insulin therapy in insulin-deficient diabetes.

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