scholarly journals Potensi Ekstrak Atuna racemosa sebagai Anti - Methicillin Resistant Staphylococcus aureus (MRSA)

2017 ◽  
Vol 35 (2) ◽  
pp. 260
Author(s):  
Siti Isrina Oktavia Salasia ◽  
Novra Arya Sandi ◽  
Fajar Budi Lestari ◽  
Verda Farida ◽  
Nurbani Aziz
Keyword(s):  
Staphylococcus Aureus ◽  
Meca Gene ◽  
New Drugs ◽  
Diffusion Test ◽  
Methicillin Resistant ◽  
Causative Agents ◽  
Mrsa Infection ◽  
Polymerase Chain ◽  
Mrsa Strains ◽  
Multiple Antibiotics

Staphylococcus aureus is one of the major causative agents of mastitis in animals and a variety of human diseases such as septicemia, endocarditis, arthritis dan osteomyelitis. Infection of Methicillin-resistant S. aureus (MRSA) has been widely reported and these strains are usually resistant to multiple antibiotics. The purpose of this study was to evaluate the potential of Atuna racemosa, as an alternative herbal medicine against MRSA infection. The MRSA strains were isolated from human and confirmed based on their resistant to various antibiotics and analyzing of the mecA gene by polymerase chain reaction (PCR). Atuna racemosa originated from Ambon, Maluku, Indonesia, were extracted using 70% ethanol. The activities of the Atuna racemosa extract against MRSA were performed by diffusion disc agar and dilussion agar tests. The results showed that Atuna racemosa extract has the barrier effect of MRSA growth at a concentration of 5% in the diffusion test and at a concentration of 7% in the dilution test. Atuna racemosa could be used as an alternative new drugs with dose of 0.07 g/ml (7%) against MRSA which is multi-resistant to many antibiotics. 

scholarly journals Screening for Methicillin-Resistant Staphylococcus aureus Colonization Using Sponges

2015 ◽  
Vol 36 (1) ◽  
pp. 28-33 ◽  
Author(s):  
Chang-Seop Lee ◽  
Bianca Montalmont ◽  
Jessica A. O’Hara ◽  
Alveena Syed ◽  
Charma Chaussard ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Nasal Swab ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Type Ii ◽  
Chain Reaction ◽  
Methicillin Resistant ◽  
Nasal Swabs ◽  
Positive Specimen ◽  
Mrsa Infection ◽  
Polymerase Chain

OBJECTIVENasal swab culture is the standard method for identifying methicillin-resistant Staphylococcus aureus (MRSA) carriers. However, this method is known to miss a substantial portion of those carrying MRSA elsewhere. We hypothesized that the additional use of a sponge to collect skin culture samples would significantly improve the sensitivity of MRSA detection.DESIGNHospitalized patients with recent MRSA infection were enrolled and underwent MRSA screening of the forehead, nostrils, pharynx, axilla, and groin with separate swabs and the forehead, axilla, and groin with separate sponges. Staphylococcal cassette chromosome mec (SCCmec) typing was conducted by polymerase chain reaction (PCR).PATIENTSA total of 105 MRSA patients were included in the study.RESULTSAt least 1 specimen from 56.2% of the patients grew MRSA. Among patients with at least 1 positive specimen, the detection sensitivities were 79.7% for the swabs and 64.4% for the sponges. Notably, 86.4% were detected by a combination of sponges and nasal swab, and 72.9% were detected by a combination of pharyngeal and nasal swabs, whereas only 50.9% were detected by nasal swab alone (P<0.0001 and P=0.0003, respectively). Most isolates had SCCmec type II (59.9%) and IV (35.7%). No correlation was observed between the SCCmec types and collection sites.CONCLUSIONScreening using a sponge significantly improves MRSA detection when used in addition to screening with the standard nasal swab.Infect Control Hosp Epidemiol 2014;36(1): 28–33

scholarly journals Comparative Genomics of Vancomycin-Resistant Staphylococcus aureus Strains and Their Positions within the Clade Most Commonly Associated with Methicillin-Resistant S. aureus Hospital-Acquired Infection in the United States

mBio ◽  
2012 ◽  
Vol 3 (3) ◽  
Author(s):  
Veronica N. Kos ◽  
Christopher A. Desjardins ◽  
Allison Griggs ◽  
Gustavo Cerqueira ◽  
Andries Van Tonder ◽  
...  
Keyword(s):  
United States ◽  
Staphylococcus Aureus ◽  
The United States ◽  
Methicillin Resistant ◽  
Content Type ◽  
Mrsa Infection ◽  
Vancomycin Resistant ◽  
Foreign Dna ◽  
Mrsa Strains ◽  
Hospital Acquired

ABSTRACTMethicillin-resistantStaphylococcus aureus(MRSA) strains are leading causes of hospital-acquired infections in the United States, and clonal cluster 5 (CC5) is the predominant lineage responsible for these infections. Since 2002, there have been 12 cases of vancomycin-resistantS. aureus(VRSA) infection in the United States—all CC5 strains. To understand this genetic background and what distinguishes it from other lineages, we generated and analyzed high-quality draft genome sequences for all available VRSA strains. Sequence comparisons show unambiguously that each strain independently acquired Tn1546and that all VRSA strains last shared a common ancestor over 50 years ago, well before the occurrence of vancomycin resistance in this species. In contrast to existing hypotheses on what predisposes this lineage to acquire Tn1546, the barrier posed by restriction systems appears to be intact in most VRSA strains. However, VRSA (and other CC5) strains were found to possess a constellation of traits that appears to be optimized for proliferation in precisely the types of polymicrobic infection where transfer could occur. They lack a bacteriocin operon that would be predicted to limit the occurrence of non-CC5 strains in mixed infection and harbor a cluster of unique superantigens and lipoproteins to confound host immunity. A frameshift indprA, which in other microbes influences uptake of foreign DNA, may also make this lineage conducive to foreign DNA acquisition.IMPORTANCEInvasive methicillin-resistantStaphylococcus aureus(MRSA) infection now ranks among the leading causes of death in the United States. Vancomycin is a key last-line bactericidal drug for treating these infections. However, since 2002, vancomycin resistance has entered this species. Of the now 12 cases of vancomycin-resistantS. aureus(VRSA), each was believed to represent a new acquisition of the vancomycin-resistant transposon Tn1546from enterococcal donors. All acquisitions of Tn1546so far have occurred in MRSA strains of the clonal cluster 5 genetic background, the most common hospital lineage causing hospital-acquired MRSA infection. To understand the nature of these strains, we determined and examined the nucleotide sequences of the genomes of all available VRSA. Genome comparison identified candidate features that position strains of this lineage well for acquiring resistance to antibiotics in mixed infection.

scholarly journals Staphylococcus aureus in poultry, with special emphasis on methicillin-resistant strain infection: A comprehensive review from one health perspective

2021 ◽  
pp. 257-267
Author(s):  
Wafaa A. Abd El-Ghany
Keyword(s):  
Staphylococcus Aureus ◽  
Prevention And Control ◽  
Close Contact ◽  
Control Strategies ◽  
Infected Individual ◽  
Surface Proteins ◽  
Methicillin Resistant ◽  
Mrsa Infection ◽  
Mrsa Strains ◽  
And Control

Staphylococcus aureus is a Gram-positive coccus normally present on the skin and internal organs of animals, birds, and humans. Under certain conditions, S. aureus could produce septicemia and affection of the skin, joints, and heart, as well as sepsis and death. The pathogenicity of S. aureus is associated with the presence of some virulent surface proteins and the production of some virulent toxins and enzymes. This pathogen is considered one of the most important and worldwide foodborne causes as it is incriminated in most cases of food poisoning. The hazardous use of antibiotics in the veterinary field leads to the development of multidrug-resistant S. aureus strains that can be transmitted to humans. The incidence of methicillin-resistant S. aureus (MRSA) strains has increased globally. These resistant strains have been detected in live animals, poultry, and humans. In addition, retail animal products, especially those of avian origin, are considered the main source of MRSA strains that can be easily transmitted to humans. MRSA infection is regarded as nosocomial or occupational. Humans get infected with MRSA strains through improper handling or preparation of contaminated animals or poultry carcasses or improper cooking with contaminated meat. Live birds also can transmit MRSA to close-contact workers in poultry farms. Transmission of MRSA infection in hospitals is from an infected individual to a healthy one. Prevention and control of MRSA are based on the application of hygienic measures in farms as well as proper processing, handling, and cooking of retail poultry products. The cooperation between veterinary and human practitioners is a must to avoid the possibility of zoonotic transmission. Accordingly, this review focused on the sources and transmission of MRSA infection, virulence and resistance factors, incidence and prevalence in poultry and different products, antibiotic resistance, and prevention and control strategies.

Community-Acquired Methicillin-Resistant Staphylococcus Aureus Infections in Otolaryngology

1992 ◽  
Vol 107 (3) ◽  
pp. 434-437 ◽  
Author(s):  
Ron D. Gottlieb ◽  
Mahendra K. Shah ◽  
David C. Perlman ◽  
Charles P. Kimmelman
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Therapy ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Intravenous Drug ◽  
Intravenous Drug Use ◽  
Methicillin Resistant ◽  
Mrsa Strains ◽  
Hospital Acquired ◽  
Susceptibility And Resistance ◽  
Multiple Antibiotics

Soon after the introduction of methicillin, strains of Staphylococcus aureus resistant to methicillin were reported. Methicillin-resistant Staphylococcus aureus (MRSA) has become a common hospital pathogen, often resistant to multiple antibiotics, while causing significant morbidity and mortality. Community-acquired MRSA infections have been infrequently documented. Most reports have been associated with intravenous drug abuse. This report reviews 15 patients with community-acquired MRSA infections of the head and neck. None admitted to intravenous drug use. Additionally, no patient was known to be a healthcare worker. The MRSA strains showed antibiotic susceptibility and resistance profiles different from typical hospital-acquired MRSA isolates. All but one infection resolved with adequate surgical or appropriate antibiotic therapy. Clinicians should become aware of the possibility of community-acquired MRSA in the patient who has had continued infection despite antibiotic therapy.

scholarly journals Commercially Distributed Meat as a Potential Vehicle for Community-Acquired Methicillin-Resistant Staphylococcus aureus

2012 ◽  
Vol 78 (8) ◽  
pp. 2797-2802 ◽  
Author(s):  
Kikuyo Ogata ◽  
Hiroshi Narimatsu ◽  
Masahiro Suzuki ◽  
Wataru Higuchi ◽  
Tatsuo Yamamoto ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Drug Susceptibility ◽  
Methicillin Resistant ◽  
Content Type ◽  
Reading Frame ◽  
Type Iv ◽  
Mrsa Infection ◽  
Stool Samples ◽  
Mrsa Strains ◽  
Meat Samples

ABSTRACTThe incidence of community-acquired methicillin-resistantStaphylococcus aureus(CA-MRSA) infection has been increasing; however, the sources of infection remain unclear. Therefore, we investigated the involvement of meat as a possible mediator of CA-MRSA infection. We examined the distribution of MRSA strains in commercially distributed raw meat samples (n= 197) and diarrheal stool samples of outpatients (n= 1,287) that were collected in Oita Prefecture, Japan, between 2003 and 2009 for routine legal inspections. Fourteen MRSA strains were isolated from three meat and 11 stool samples. Among these, seven isolates from three meat and four stool samples exhibited the same epidemiological marker profiles [coagulase type III, staphylococcal enterotoxin C, staphylococcal chromosomal cassettemec(SCCmec) type IV, ST8,spatype 606 (t1767), and toxic shock syndrome toxin-1 (TSST-1) producing type]. Furthermore, of the seven strains, three isolates from two meat samples and one stool sample collected in 2007 exhibited completely identical characteristics with respect to phage open reading frame (ORF) typing, pulsed-field gel electrophoresis, and drug susceptibility profiles. The results suggest that commercially distributed meat could play a role in the prevalence of CA-MRSA in the community.

scholarly journals Characteristics of Erythromycin Resistance in Methicillin-Resistant Staphylococcus aureus Isolated From Raw Milk

2019 ◽  
Vol 7 (4) ◽  
pp. 121-125
Author(s):  
Fatemeh Mahdavi ◽  
Fatemeh Zaboli ◽  
Rahem Khoshbakht
Keyword(s):  
Staphylococcus Aureus ◽  
Resistance Genes ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Methicillin Resistance ◽  
Meca Gene ◽  
Raw Milk ◽  
Erythromycin Resistance ◽  
Methicillin Resistant ◽  
Inducible Clindamycin Resistance ◽  
Mrsa Strains

Background: Methicillin-resistant Staphylococcus aureus (MRSA) strains are one of the most important multidrug resistant microorganisms that threaten human health. Objective: The present study was conducted to evaluate genotypic and phenotypic characteristics of erythromycin resistance among MRSA isolates recovered from raw milk in Iran. Materials and Methods: A total of 50 MRSA isolates were recovered from raw milk. Tests for erythromycin and clindamycin susceptibility and inducible clindamycin resistance were done. In addition, the presence of the methicillin resistance determinant (mecA), erythromycin resistance genes (ermA, ermB, ermC and msrA) and an important virulence gene (Panton– Valentine leukocidin) were investigated using polymerase chain reaction (PCR) method. Results: Forty-eight percent (24/50) and 46% (23/50) of the isolates were resistant to erythromycin and clindamycin, respectively. Seven (14%) isolates showed inducible clindamycin resistance phenotype. The mecA gene was detected in 88% (44/50) of MRSA isolates. The incidence of the ermA, ermB, ermC and msrA genes was 14%, 64%, 12%, and 26%, respectively and the PVL gene was present in 18% (9/50) of MRSA isolates. Conclusion: According to the results of the study, the incidence of erythromycin resistance genes and inducible clindamycin-resistant MRSA strains was high in raw milk samples in Iran.

scholarly journals Frequencies and Antimicrobial Susceptibility of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolated from Horses in South Korea

2018 ◽  
Vol 12 (1) ◽  
pp. 148-155
Author(s):  
Seoung-Kyoon Choi ◽  
Ji-Yong Hwang ◽  
Chul-Song Park ◽  
Gil-Jae Cho
Keyword(s):  
Staphylococcus Aureus ◽  
South Korea ◽  
Antimicrobial Susceptibility ◽  
Test Method ◽  
Methicillin Resistant ◽  
Treatment And Prevention ◽  
Horse Riding ◽  
Horse Farms ◽  
Mrsa Infection ◽  
Mrsa Strains

Background:Methicillin-ResistantStaphylococcus Aureus(MRSA) has become one of the most prevalent pathogens in animals and humans giving rise to various diseases. MRSA infection in horses and transmission between horses and humans have dramatically increased recently.Objective:This study investigated the isolation frequency and antimicrobial susceptibility of MRSA isolated from horses in South Korea.Method:Screening of the MRSA isolates was conducted by conventional methods and multiplex Polymerase Chain Reaction (PCR). Minimum Inhibitory Concentration (MIC) of oxacillin was determined by the broth microdilution test method. Overall antibiogram was obtained by disk diffusion susceptibility test. All antimicrobial tests were conducted in accordance with the Clinical and Laboratory Standards Institute (CLSI) guidelines.Results:S. aureuswas isolated from 116 (19.46%) of 596 horses tested. Of the 116S. aureusisolates, 52 (8.72%) strains were identified as MRSA by multiplex PCR. Among the 52 MRSA strains, 24 (46.15%) and 28 (53.85%) were oxacillin-resistant MRSA (OR-MRSA) and oxacillin-susceptible MRSA (OS-MRSA), respectively. OR-MRSA showed the highest susceptibility to florfenicol (100.00%), followed by doxycycline (95.83%), cefepime (91.67%), tetracycline (75.00%), and trimethoprim/sulphamethoxazole (70.83%). OS-MRSA showed the highest susceptibility to florfenicol (100.00%) and trimethoprim/sulphamethoxazole (100.00%), followed by cefoxitin (96.43%), ciprofloxacin (92.86%), enrofloxacin (92.86%), lincomycin + spectinomycin (89.29%), cefuroxime (89.29%), and cefonicid (89.29%).Conclusion:This study may facilitate treatment and prevention of MRSA infections and further benefit not only horses, but also people related with horse farms, horse riding clubs and animal hospitals.

scholarly journals Methicillin-Resistant Staphylococcus aureus spa Type t002 Outbreak in Horses and Staff at a Veterinary Teaching Hospital after Its Presumed Introduction by a Veterinarian

2015 ◽  
Vol 53 (9) ◽  
pp. 2827-2831 ◽  
Author(s):  
Amir Steinman ◽  
Samira Masarwa ◽  
Sharon Tirosh-Levy ◽  
Dan Gleser ◽  
Gal Kelmer ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Teaching Hospital ◽  
Large Animal ◽  
Prevalence Survey ◽  
Methicillin Resistant ◽  
Content Type ◽  
Staff Members ◽  
Cross Transmission ◽  
Mrsa Infection ◽  
Mrsa Strains

Methicillin-resistantStaphylococcus aureus(MRSA) infection and colonization, involving MRSA strains which differ from common human health care-associated clones, have become serious emerging conditions in equine veterinary hospitals. In 2010, MRSAspatype t535 caused an outbreak involving both horses and personnel in a veterinary teaching hospital in Israel. Since then, surveillance continued, and occasional MRSA isolation occurred. Two years later, MRSA of anotherspatype, t002, was isolated from a veterinarian and, 3 weeks later, from a horse. The appearance ofspatype t002, a common clone in human medicine in Israel, among both personnel and horses, prompted a point-prevalence survey of hospital personnel and hospitalized horses. Fifty-nine staff members (n= 16 equine;n= 43, other) and 14 horses were screened. Ten of 59 staff members (16.9%) and 7 of 14 horses (50%) were MRSA carriers. Among the staff, 44% of large animal department (LAD) personnel, compared with only 7% of non-LAD personnel, were carriers. Isolates from all horses and from 9 of 10 personnel were found to be of MRSAspatype t002. This clone was later isolated from an infected postoperative wound in a hospitalized horse. Measures were taken to contain transmission between horses and personnel, as was done in the previous outbreak, resulting in reduction of transmission and, finally, cessation of cross-transmission between horses and personnel.

Changing epidemiology of methicillin-resistantStaphylococcus aureusin Alberta, Canada: population-based surveillance, 2005–2008

2010 ◽  
Vol 139 (7) ◽  
pp. 1009-1018 ◽  
Author(s):  
J. KIM ◽  
C. FERRATO ◽  
G. R. GOLDING ◽  
M. R. MULVEY ◽  
K. A. SIMMONDS ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Susceptibility ◽  
Population Based ◽  
Age Group ◽  
Prospective Surveillance ◽  
Methicillin Resistant ◽  
Source Of Infection ◽  
Mrsa Infection ◽  
Mrsa Strains ◽  
Predominant Strain

SUMMARYIncreasing prevalence of methicillin-resistantStaphylococcus aureus(MRSA) has been reported in Canada. We report the results of a prospective surveillance of MRSA infections in Alberta over a consecutive 3-year period. A total of 8910 unique clinical MRSA isolates was analysed from July 2005 to June 2008. The incidence of MRSA infection increased over the study period and was highest in males, age group ⩾85 years, and the Calgary Area. CMRSA10 (USA300) and CMRSA2 (USA100/800) were the most common PFGE strain types, representing 53·0% and 27·9% of all isolates, respectively. Significant differences were noted between MRSA strains in the source of infection and antimicrobial susceptibility. The incidence of MRSA infection in Alberta has nearly doubled in the last 3 years; this is attributed to the emergence of CMRSA10 as the predominant strain.

Sign in / Sign up

Export Citation Format

Share Document