scholarly journals IN VITRO CYTOTOXIC STUDY OF GREEN SYNTHESIZED GOLD AND SILVER NANOPARTICLES USING ECLIPTA PROSTRATA (L.) AGAINST HT-29 CELL LINE

2016 ◽  
Vol 9 (5) ◽  
pp. 189 ◽  
Author(s):  
Arvindganth Rajashekar ◽  
Vardhana Janakiraman ◽  
Kathiravan Govindarajan
Keyword(s):  
Silver Nanoparticles ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Cell Line ◽  
Plant Extract ◽  
Biological Synthesis ◽  
Gold And Silver Nanoparticles ◽  
Eclipta Prostrata ◽  
Ht 29 ◽  
Scanning Electron

ABSTRACTObjective: The objective of this study is to synthesize gold and silver nanoparticles using the extracts of Eclipta prostrata.Methods: The nanoparticles synthesize is carried out using the powdered leaves and mixed with distilled water. The filtered extract was then mixedwith aqueous solution of HAuCl4 (1 mM)and AgNo(1 mM), and the reaction volume was made up to 100 ml. Then, the characterization of nanoparticleswas carried out using ultraviolet, infrared, scanning electron microscope, and the cytotoxic activity of the nanoparticles were investigated against HT29cancer cell lines. 3 Results: From the study, it was found that the plant extract was able to synthesize nanoparticles, and the synthesized nanoparticles were found to betoxic against the cancer cell line HT-29.Conclusion: In the present study, both silver and gold nanoparticles were synthesized using the plant extract of E. prostrata. The synthesizednanoparticles were found to be effective against HT 29 cancer cells. The green synthesized nanoparticles were found to be cost-effective, simpler andenvironmentally safe. As the nanotechnology is an emerging field in medicine, the biological synthesis of nanoparticles helps in the other way. Fromthe present study, the nanoparticles synthesized were thus proved against various studies novely. Hope this paves way for the better development ofnanoparticle production in the large scale amount.Keywords: Nanoparticles, Eclipta prostrata, Scanning electron microscope, HT-29 cell line. §Both authors are equally contributed

scholarly journals SILVER NANOPARTICLES FROM MEDICINALLY IMPORTANT EUPHORBIA CYATHOPHORA EXTRACT: BIOSYNTHESIS, CHARACTERIZATION, AND ANTICANCER ACTIVITY.

2018 ◽  
Vol 11 (2) ◽  
pp. 154
Author(s):  
Robert Lotha ◽  
Aravind Sivasubramanian ◽  
Meenakshi Sundaram Muthuraman
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Cell Line ◽  
Aqueous Extract ◽  
Visible Spectroscopy ◽  
X Ray ◽  
Transmission Electron ◽  
The Stability ◽  
Ht 29 ◽  
Scanning Electron

Objective: The present study was aimed at the biosynthesis of silver nanoparticles (AgNPs) using aqueous extract of Euphorbia cyathophora leavesand testing their anticancer potential using HT-29 cell line model.Methods: Green synthesis of silver nanoparticles was obtained with the aqueous extract of E. cyathophora. The synthesized nanoparticles wereconfirmed initially by ultraviolet-visible spectroscopy. Further, scanning electron microscopy, transmission electron microscopy, and X-Ray diffractionstudies also ensured the presence of silver nanoparticles. Zeta potential studies revealed the stability of the silver nanoparticles.Results: Antioxidant and anticancer studies of the nanoparticles against HT-29 cell line exhibited remarkable results.Conclusion: This ensures that the synthesized nanoparticles play an important role in medicinal biology.

scholarly journals Aktivitas Antibakteri Membran Nanokomposit Kitosan/Nanopartikel Perak

ALCHEMY ◽  
2021 ◽  
Vol 9 (1) ◽  
pp. 26-31
Author(s):  
Doni Notriawan ◽  
Nesbah Nesbah ◽  
Gustria Ernis ◽  
Muhammad Adeng Fadhila ◽  
Risky Hadi Wibowo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Nanocomposite Membrane ◽  
Nanocomposite Membranes ◽  
E Coli ◽  
Brucea Javanica ◽  
Chitosan Membrane ◽  
Scanning Electron

The aim of this study was to make and test the antibacterial activity of chitosan/silver nanoparticles nanocomposite membranes. Nanocomposite membranes were synthesized by chitosan with pluronic using acetic acid as a solvent. Silver nanoparticles were synthesized using the green synthesis method and the makasar fruit (Brucea javanica L. Merr) peel extract as a bioreductor. The silver nanoparticles were composited with a chitosan/pluronic mixture and printed on a glass plate. Nanocomposite membranes were characterized using the FTIR spectrophotometer and the scanning electron microscope (SEM). Nanocomposite membrane were tested for antibacterial activity against Escherechia coli. The UV-Vis spectra showed the formation of silver nanoparticles which were indicated by the absorption at 454 nm and the absorbance value of 0.405. Characterization using FTIR showed no new functional groups formed in the composites of chitosan and pluronic. SEM results showed the difference between the chitosan membrane and the nanocomposite membrane. The surface of nanocomposite membrane showed uneven compared to the chitosan membrane. Nanocomposite membranes have antibacterial activity to inhibit E. coli growth.   Keywords: nanocomposite, chitosan/silver nanoparticles, antibacterial Penelitian ini bertujuan untuk membuat dan menguji aktivitas antibakteri membran nanokomposit kitosan/nanopartikel perak. Membran nanokomposit dibuat dengan mensintesis kitosan dengan pluronik menggunakan pelarut asam asetat. Nanopartikel perak disintesis menggunakan metode green synthesis dengan ekstrak kulit buah makasar (Brucea javanica L. Merr) sebagai bioreduktor. Nanopartikel perak dikompositkan dengan campuran kitosan/pluronik dan dicetak di atas plat kaca. Membran nanokomposit dikarakterisasi menggunakan spektrofotometer FTIR dan scanning electron microscope (SEM). Membran nanokomposit dilakukan uji aktivitas antibakteri terhadap Escherechia coli. Spektra UV-Vis menunjukkan terbentuknya nanopartikel perak yang ditandai adanya serapan pada panjang gelombang 454 nm dan absorbansi 0,405. Karakterisasi dengan FTIR menunjukkan tidak adanya gugus fungsi baru yang terbentuk pada komposit dari kitosan dan pluronik. Hasil SEM menunjukkan adanya perbedaan antara membran kitosan dengan membran nanokomposit. Permukaan membran nanokomposit terlihat tidak rata dibandingkan membran kitosan. Membran nanokomposit memiliki aktivitas bakteri sehingga dapat mengambat pertumbuhan bakteri E. coli. Kata kunci: nanokomposit, kitosan/nanopartikel perak, antibakteri

scholarly journals In-Situ Biofabrication of Silver Nanoparticles in Ceiba pentandra Natural Fiber Using Entada spiralis Extract with Their Antibacterial and Catalytic Dye Reduction Properties

Nanomaterials ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 1104 ◽  
Author(s):  
Wan Khaima Azira Wan Mat Khalir ◽  
Kamyar Shameli ◽  
Seyed Davoud Jazayeri ◽  
Nor Azizi Othman ◽  
Nurfatehah Wahyuny Che Jusoh ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Natural Fiber ◽  
Ag Nps ◽  
X Ray ◽  
Ceiba Pentandra ◽  
Dye Reduction ◽  
Scanning Electron

It is believed of great interest to incorporate silver nanoparticles (Ag-NPs) into stable supported materials using biological methods to control the adverse properties of nanoscale particles. In this study, in-situ biofabrication of Ag-NPs using Entada spiralis (E. spiralis) aqueous extract in Ceiba pentandra (C. pentandra) fiber as supporting material was used in which, the E. spiralis extract acted as both reducing and stabilizing agents to incorporate Ag-NPs in the C. pentandra fiber. The properties of Ag-NPs incorporated in the C. pentandra fiber (C. pentandra/Ag-NPs) were characterized using UV-visible spectroscopy (UV-vis), X-ray Diffraction (XRD), Field Emission Transmission Electron Microscope (FETEM), Scanning Electron Microscope (Scanning Electron Microscope (SEM), Energy Dispersive X-ray (EDX), Brunauer-Emmett-Teller (BET), Thermogravimetric (TGA) and Fourier Transform Infrared (FTIR) analyses. The average size of Ag-NPs measured using FETEM image was 4.74 nm spherical in shape. The C. pentandra/Ag-NPs was easily separated after application, and could control the release of Ag-NPs to the environment due to its strong attachment in C. pentandra fiber. The C. pentandra/Ag-NPs exposed good qualitative and quantitative antibacterial activities against Staphylococcus aureus (ATCC 25923), Enterococcus faecalis (ATCC 29212), Escherichia coli (ATCC 25922) and Proteus vulgaris (ATCC 33420). The dye catalytic properties of C. pentandra/Ag-NPs revealed the dye reduction time in which it was completed within 4 min for 20 mg/L rhodamine B and 20 min for 20 mg/L methylene blue dye, respectively. Based on the results, it is evident that C. pentandra/Ag-NPs are potentially promising to be applied in wound healing, textile, wastewater treatment, food packaging, labeling and biomedical fields.

Synthesis of Silver Nanoparticles by Sparking Process

2016 ◽  
Vol 675-676 ◽  
pp. 73-76
Author(s):  
Thanut Jintakosol
Keyword(s):  
Raman Spectroscopy ◽  
Silver Nanoparticles ◽  
Optical Properties ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Low Cost ◽  
Ag Nps ◽  
Spherical Structure ◽  
Atomic Force ◽  
Scanning Electron

Silver nanoparticles (Ag NPs) were synthesized by sparking process. The NPs were continuously deposited onto the substrate by varying the number of sparking cycles ranging from 100-200 and deposited on the glass substrate and annealing at 250-500 °C for 1 hr. The effect of Ag NPs on their structure and optical characteristics were investigated. The optical properties of the NPs have been studied from by Raman spectroscopy and UV-vis spectrophotometer. The morphology of Ag NP was characterized by Scanning Electron Microscope (SEM) and Atomic force microscope (AFM). The results have shown mainly spherical structure of Ag NPs in diameter ranging from 80-100 nm. This study demonstrated that the Ag NPs can be synthesized by sparking process and simply with low cost.

scholarly journals Anti-Corrosive Behavior of the Seed Extract of Amomum sabulatum

2021 ◽  
Vol 309 ◽  
pp. 01026
Author(s):  
Neha Daksh ◽  
Akhil Saxena ◽  
Jayahari Lade ◽  
Nakul Gupta
Keyword(s):  
Weight Loss ◽  
Electron Microscope ◽  
Corrosion Rate ◽  
Scanning Electron Microscope ◽  
Mild Steel ◽  
Plant Extract ◽  
Inhibition Efficiency ◽  
Protective Layer ◽  
Seed Extract ◽  
Scanning Electron

An aqueous extract of Amomum sabulatum seeds was evaluated for anticorrosive effects on mild steel in the presence of HCl. Weight loss measurements were taken to determine the corrosion rate and inhibition efficiency at various concentrations. With the increasing concentrations of plant extract, the results showed an improvement in inhibition efficiency and a decrease in corrosion rate. In the presence of 1500 ppm concentration of plant extract, it has an inhibition efficiency of 82.08 % at 303 K. The scanning electron microscope was used to examine the surface, which proves that while the extract was there, a protective layer formed on the metal surface.

Fabricating Polyvinyl Alcohol Fibers with Evenly Distributed Nano Silver

2015 ◽  
Vol 827 ◽  
pp. 95-98 ◽  
Author(s):  
Harsojo ◽  
Anna Layla ◽  
Kuwat Triyana ◽  
Harini Sosiati
Keyword(s):  
Silver Nanoparticles ◽  
Electron Microscope ◽  
Optical Property ◽  
Scanning Electron Microscope ◽  
Polyvinyl Alcohol ◽  
Room Temperature ◽  
Hole Diameter ◽  
Dc Voltage ◽  
Transmission Electron ◽  
Scanning Electron

Fabrication of polyvinyl alcohol (PVA) fibers loaded with evenly distributed nanosilver has been sucessfully done using electrospinner. The electrospinner is set at 15 kV DC voltage with distance between electrodes 13 cm, using a syringe hole diameter 0,5 mm. The feeding solution for the electrospinning was prepared by directly mixing the solution of PVA in water with a stable colloid of nanosilver at room temperature. The fibers morphology was characterized using scanning electron microscope. The optical property was tested using spectrometer. The distribution of silver nanoparticles in the fibers was tested using transmission electron microscope. The result indicates that the fibers still showing plasmonic property of silver having peak at 410 nm with no crystaline changes. The diameter of fibers loaded with nanosilver are smaller compared to that of the ones without nanosilver.The distribution of nanosilver in fibers made of PVA and the ones made of PVA and chitosan are compared and discussed.

scholarly journals POTENSI EKSTRAK KULIT JERUK KUNCI (Citrus microcarpa Bunge) SEBAGAI BIOREDUKTOR DALAM SINTESIS NANOPARTIKEL PERAK

2021 ◽  
Vol 7 (1) ◽  
pp. 12-20
Author(s):  
Athiah Masykuroh ◽  
Nadia Nia Nurulita
Keyword(s):  
Silver Nanoparticles ◽  
Surface Plasmon Resonance ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Fruit Peel ◽  
Color Test ◽  
Agno3 Solution ◽  
Average Size ◽  
Peel Extract ◽  
Scanning Electron

Nowadays silver nanoparticles (AgNPs) synthesized so often by plant extracts as a reductor. The synthesis of AgNPs was carried out by Citrus microcarpa Bunge fruit peel extract a reductor in various extract concentrations (10, 15, and 20%), concentration of AgNO3 solution of 0.15M and temperature of 700C. The presence of AgNPs was determined by color test and the formation of Surface Plasmon Resonance (SPR) using UV-Vis Spectrophotometer while to determine the morphology and size of the nanoparticles using Scanning Electron  Microscope (SEM). The results of the analysis showed that AgNPs was formed at colloidal phase with dark brown color with wavelengths of 457.30 nm, 478.90 nm, and 422.80 nm for variation concentration of 10, 15 and 20% with slightly spherical, slightly elongated and jagged morphology with average size of 253.8 nm (10%), 254.2 nm (15%) and 253.9 nm (20%).

scholarly journals Formation of planar plasmon microstructures by dry aerosol printing

2021 ◽  
Vol 2086 (1) ◽  
pp. 012147
Author(s):  
K M Khabarov ◽  
M Nouraldeen ◽  
A A Lizunova ◽  
M N Urazov ◽  
V V Ivanov
Keyword(s):  
Silver Nanoparticles ◽  
Optical Properties ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Transmission Electron Microscope ◽  
Q Factor ◽  
Transmission Electron ◽  
Aerosol Printing ◽  
Individual Nanoparticles ◽  
Scanning Electron

Abstract Optical properties and microstructure of samples formed by dry aerosol printing are studied. Silver nanoparticles flat layers of two types were formed on substrates surfaces and were investigated by a spectrophotometer, a scanning electron microscope, and a transmission electron microscope. It is shown that all microstructures support plasmon resonance on individual nanoparticles with the Q factor depending both on the width of the nanoparticles size distribution in the aerosol and on their tendency to agglomeration and aggregation.

The Alteration of the Pore Spaces in Sandstones

1973 ◽  
Vol 31 ◽  
pp. 210-211
Author(s):  
R. E. Ferrell ◽  
G. G. Paulson
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
The Other ◽  
Coarse Grained ◽  
Depositional Fabric ◽  
Soluble Components ◽  
Scanning Electron ◽  
Shape And Size

The pore spaces in sandstones are the result of the original depositional fabric and the degree of post-depositional alteration that the rock has experienced. The largest pore volumes are present in coarse-grained, well-sorted materials with high sphericity. The chief mechanisms which alter the shape and size of the pores are precipitation of cementing agents and the dissolution of soluble components. Each process may operate alone or in combination with the other, or there may be several generations of cementation and solution.The scanning electron microscope has ‘been used in this study to reveal the morphology of the pore spaces in a variety of moderate porosity, orthoquartzites.

Sign in / Sign up

Export Citation Format

Share Document