scholarly journals PRODUCTION OF KERATINASES FROM NOCARDIOPSIS SP. 28ROR AS A NOVEL IRAQI STRAIN

2017 ◽  
Vol 10 (4) ◽  
pp. 160
Author(s):  
Rabab Omran Al-jelawi-
Keyword(s):  
Potential Application ◽  
Skim Milk ◽  
Gene Analysis ◽  
Poultry Farm ◽  
Chicken Feather ◽  
Feather Meal ◽  
Skim Milk Agar ◽  
16S R Rna ◽  
Acid Type

Objectives: isolate a novel feather- degrading actinobacterial species had the ability to produce wide pH activity keratinases.Methods: Of 23 actinobacterial isolates were recovered from farm soil, poultry farm soil and feather wastes, these isolates were screened for protease and keratinase production on skim milk agar, feather  media, and  antimicrobial production. One potential  isolate was identified depending on phenotypical, physiological and molecular according to partial sequences of 16S r RNA gene analysis and optimized  keratinase production. Results:   11 isolates out of 22 protease producer  had the ability to degrade raw chicken feather and some of these  isolates produced  antifungal and antibacterial metabolites.The potential isolate,  Nocardiopsis sp. 28ROR (GenBank: KC702802.1), produced two types of extracellular keratinases in feather meal  medium at pH6 (acid type), 30-35°C  for 7d  and  the alkaline keratinase at pH10, 40°C  for 7d.Conclusion: The Nocardiopsis sp. 28ROR was a novel strain produced keratinases using feather meal degradation as a cheap waste medium. The wide tolerance of temperature and pH by keratinase makes it an ideal contender to be investigated further for potential application as a detergent additive.Keywords: Nocardiopsis, Keratinase, Optimization, Feather medium, Antibiotic. 

scholarly journals Complex regulation of AprA metalloprotease in Pseudomonas fluorescens M114: evidence for the involvement of iron, the ECF sigma factor, PbrA and pseudobactin M114 siderophore

Microbiology ◽  
2006 ◽  
Vol 152 (1) ◽  
pp. 29-42 ◽  
Author(s):  
Bláithín Maunsell ◽  
Claire Adams ◽  
Fergal O'Gara
Keyword(s):  
Pseudomonas Fluorescens ◽  
Proteolytic Activity ◽  
Yeast Extract ◽  
Sigma Factor ◽  
Skim Milk ◽  
Protease Production ◽  
Iron Starvation ◽  
Transcription Analysis ◽  
Skim Milk Agar ◽  
Complex Regulation

In the soil bacterium Pseudomonas fluorescens M114, extracellular proteolytic activity and fluorescent siderophore (pseudobactin M114) production were previously shown to be co-ordinately negatively regulated in response to environmental iron levels. An iron-starvation extracytoplasmic function sigma factor, PbrA, required for the transcription of siderophore biosynthetic genes, was also implicated in M114 protease regulation. The current study centred on the characterization and genetic regulation of the gene(s) responsible for protease production in M114. A serralysin-type metalloprotease gene, aprA, was identified and found to encode the major, if not only, extracellular protease produced by this strain. The expression of aprA and its protein product were found to be subject to complex regulation. Transcription analysis confirmed that PbrA was required for full aprA transcription under low iron conditions, while the ferric uptake regulator, Fur, was implicated in aprA repression under high iron conditions. Interestingly, the iron regulation of AprA was dependent on culture conditions, with PbrA-independent AprA-mediated proteolytic activity observed on skim milk agar supplemented with yeast extract, when supplied with iron or purified pseudobactin M114. These effects were not observed on skim milk agar without yeast extract. PbrA-independent aprA expression was also observed from a truncated transcriptional fusion when grown in sucrose asparagine tryptone broth supplied with iron or purified pseudobactin M114. Thus, experimental evidence suggested that iron mediated its effects via transcriptional activation by PbrA under low iron conditions, while an as-yet-unidentified sigma factor(s) may be required for the PbrA-independent aprA expression and AprA proteolytic activity induced by siderophore and iron.

scholarly journals Use of chicken feather meal fermented with Bacillus subtilis in diets to increase the digestive enzymes activity and nutrient digestibility of silver pompano Trachinotus blochii (Lacepede, 1801)

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 25
Author(s):  
Adelina Adelina ◽  
Feli Feliatra ◽  
Yusni Ikhwan Siregar ◽  
Iskandar Putra ◽  
Indra Suharman
Keyword(s):  
Bacillus Subtilis ◽  
Digestive Enzymes ◽  
Nutrient Digestibility ◽  
Histological Structure ◽  
Fish Feed ◽  
Chicken Feather ◽  
Feather Meal ◽  
Average Body ◽  
Chicken Feather Meal ◽  
Trachinotus Blochii

Background: Feather has the potential to be used as a fish feed ingredient because it has high protein content (80-85%), and is rich in amino acids arginine, leucine, isoleucine and valine. However, the protein consists mainly of keratin, which is classified as fiber that is difficult to digest. Therefore, to improve digestibility, the keratin protein is degraded using microbial Bacillus subtilis. This study aimed to determine the digestibility of fermented feather meal (FFM) in silver pompano diets and to observe the histological structure of their intestines after digestion. Methods: The method used was a one factor experiment with five treatments and three replications each, which were: diet without FFM (P0), diet containing 10% FFM (P1), 20% (P2), 30% (P3) and 40% (P4). The diets were given to juvenile silver pompano (with average body weight of 8.56 ± 0.18 g) and stocked with 10 fish in a 100 L capacity container. The experimental diets were given three times daily at approximately 8.00 AM, 12.00 PM and 5.00 PM to apparent satiation for 60 days. Results: The results showed that the use of FFM increased the activity of digestive enzymes (protease and lipase), but reduced the amylase activity of silver pompano, which was significantly different between treatments (P <0.05). Meanwhile, the diet containing 20% FFM produced the highest feed and protein, which are 37.05% and 67.24%, respectively. This was significantly different from other treatments (P <0.05), and was effectively absorbed by fish intestines. Conclusion: The addition of chicken feather meal fermented with Bacillus subtilis could increase the activity of digestive enzymes and nutrient digestibility of silver pompano.

scholarly journals Substitution of Fish Meal with Chicken Feather Silage Meal on Feed Can Improve Growth Performance of Striped Catfish (Pangasius hypopthalmus)

Omni-Akuatika ◽  
2018 ◽  
Vol 14 (3) ◽  
Author(s):  
Diana Rachmawati ◽  
Istiyanto Samidjan ◽  
Dicky Harwanto ◽  
Hadi Pranggono
Keyword(s):  
Survival Rate ◽  
Fish Meal ◽  
Protein Digestibility ◽  
Average Weight ◽  
Chicken Feather ◽  
Feed Conversion ◽  
Feather Meal ◽  
Chicken Feathers ◽  
Striped Catfish ◽  
Chicken Feather Meal

The purpose of this research is to study the effect of substitution of fish meal with silage meal of chicken feathers on feed to protein digestibility, growth and survival rate of striped catfish (Pangasius hypopthalmus). The test fish used is catfish with an average weight of 2.31 ± 0.04 grams /fish. This research used experimental method with Completely Randomized Design, 5 treatments in triplicate. The treatments in this study were substitution of fish meal meal with different dose of chicken feather meal, namely A, B, C, D and E (0%; 25%; 50%; 75% and 100% silage meal of chicken feathers, respectively). Parameters observed included relative growth rate (RGR), feed conversion ratio (FCR), protein efficiency ratio (PER), Apparent Digestibility Coefficient of Protein (ADCP), and survival rate (SR) of catfish. The results showed that the substitution of fish meal with chicken feather silage meal was very significant (P <0.01) to RGR, FCR, PER, and ADCP, but no significant effect (P> 0.05) on SR of catfish. Dose 25% silage chicken feather meal in the feed is the best dose to improve the RGR and ADCP of catfish. Water quality during the study is still within a reasonable range for catfish culture.

scholarly journals Improvement of Bacillus licheniformis MZK05 by mutation for increased production of keratinase

2015 ◽  
Vol 24 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Serajus Salaheen ◽  
Md Arafat Al Mamun ◽  
Shakila Nargis Khan ◽  
Md Mozammel Hoq
Keyword(s):  
Bacillus Licheniformis ◽  
Large Scale ◽  
Skim Milk ◽  
Shake Flask Culture ◽  
Scale Production ◽  
Feather Meal ◽  
Carbon And Nitrogen ◽  
Enhanced Production ◽  
Large Scale Production ◽  
Improved Strain

Bacillus licheniformis MZK05 was subjected to mutation by ultraviolet radiation for enhanced production of keratinase. Of 750 isolates from irradiated plates, 200 colonies that showed zone of casein hydrolysis on Skim Milk Agar were cultured in liquid Feather Meal Medium containing digested feather as carbon and nitrogen source in shake culture at 37ºC. The mutant B. licheniformis MZK05M9 (BlM9) exhibited highest enzyme activity of 170 ± 5.63 U/ml as compared to 74 ± 5.29 U/ml by the wild MZK05. Both the strains were examined for the presence of gene encoded for keratinase (kerA gene) by PCR using primer which showed the product sizes 1156 bp and 520 bp, respectively for MZK05 and BlM9. The keratinase from both strains exhibited a thermal stability of about 97% for 2 hrs at 40°C whereas the keratinase of the mutant strain showed less stability (55%) at 50°C. The BlM9 while cultivated in batch culture in 7 litre bioreactor for production of the keratinase in the Feather Meal Medium, the productivity was found to be double (17,608 U/L/hr) than that of in the shake flask culture (8,525 U/L/hr). This improved strain thus will be very useful for large scale production of keratinase enabling its technical applications in industry. Dhaka Univ. J. Biol. Sci. 24(1): 17-23, 2015 (January)

Microwave assisted biodiesel production from chicken feather meal oil using Bio-Nano Calcium oxide derived from chicken egg shell

2021 ◽  
pp. 112509
Author(s):  
Minglong Zhang ◽  
Ganesan Ramya ◽  
Kathirvel Brindhadevi ◽  
Mishal Alsehli ◽  
Ashraf Elfasakhany ◽  
...  
Keyword(s):  
Calcium Oxide ◽  
Biodiesel Production ◽  
Chicken Feather ◽  
Feather Meal ◽  
Microwave Assisted ◽  
Chicken Egg ◽  
Egg Shell ◽  
Chicken Feather Meal

Enterococcus faecium 1.15 Isolated from Bakasam Showed Milk Clotting Activity

2017 ◽  
Vol 21 (1) ◽  
pp. 9 ◽  
Author(s):  
Wendry Setiyadi Putranto ◽  
Kusmajadi Suradi ◽  
Hartati Chairunnisa ◽  
Apon Zaenal Mustopa ◽  
Puspo Edi Giriwono ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
16S Rrna ◽  
Enterococcus Faecium ◽  
Skim Milk ◽  
Milk Clotting ◽  
Skim Milk Agar ◽  
Modified Method ◽  
Activity Test ◽  
Milk Clotting Activity

The Lactic Acid Bacteria with Milk Clotting Activity (MCA) were isolated from Bakasam, an Indonesian traditional fermented meat. The isolate screening was carried out using modified method of Skim Milk Agar and Milk Clotting Activity Test, and the isolate was then identified using 16S rRNA. We found 4 isolates that showed MCA of 18-20 SU/ml. Identification using 16S rRNA indicated that the isolate ALG.1.15 was 99% (FR3-F primer) and 99% (FR3-R primer) identic with Enterococcus faecium. The isolate potentially produced renin-like protease to subtitute renin from veal.  

scholarly journals The Use of Chicken Feather Meal in Feeds

1955 ◽  
Vol 34 (3) ◽  
pp. 518-524 ◽  
Author(s):  
O.H.M. Wilder ◽  
Paul C. Ostby ◽  
Barbara R. Gregory
Keyword(s):  
Chicken Feather ◽  
Feather Meal ◽  
Chicken Feather Meal
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