scholarly journals ANTIOXIDANT ACTIVITY OF RED DRAGON FRUIT PEEL (HYLOCEREUS POLYRHIZUS (F.A.C. WEBER) BRITTON AND ROSE) ISOLATES USING 2,2-DIPHENYL-1-PICRYLHYDRAZYL METHOD

2018 ◽  
Vol 11 (1) ◽  
pp. 124
Author(s):  
Sri Wahdaningsih ◽  
Subagus Wahyuono ◽  
Sugeng Riyanto ◽  
Retno Murwanti
Keyword(s):  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Medicinal Plants ◽  
Thin Layer ◽  
Preparative Thin Layer Chromatography ◽  
Fruit Peel ◽  
Dragon Fruit ◽  
Vacuum Liquid Chromatography ◽  
Layer Chromatography ◽  
Hylocereus Polyrhizus

  Objective: Indonesia is a country which has various natural resources including medicinal plants. Among the plants, red dragon fruit is interesting to be studied. In this study, antioxidant activity of red dragon fruit peel (Hylocereus polyrhizus (F.A.C. Weber) Britton and Rose) isolate was tested using 2,2-diphenyl-1-picrylhydrazyl method.Methods: Isolation of active compounds was performed through the application of vacuum liquid chromatography and preparative thin-layer chromatography (TLC). Antioxidant activity was tested using both TLC and spectrophotometry.Result: Results revealed that the values of IC50 of isolate 1 and 2 were 2.952, 14 μg/mL and 25.635,95 μg/mL, respectively.Conclusion: Isolate of dragon fruit has antioxidant activity. The compound which results in antioxidant activity is terpenoid and steroid.

scholarly journals ANTIOXIDANT ACTIVITY OF RED DRAGON FRUIT PEEL (HYLOCEREUS POLYRHIZUS (F.A.C. WEBER) BRITTON AND ROSE) ISOLATES USING 2,2-DIPHENYL-1-PICRYLHYDRAZYL METHOD

2018 ◽  
Vol 11 (1) ◽  
pp. 124 ◽  
Author(s):  
Sri Wahdaningsih ◽  
Subagus Wahyuono ◽  
Sugeng Riyanto ◽  
Retno Murwanti
Keyword(s):  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Medicinal Plants ◽  
Thin Layer ◽  
Preparative Thin Layer Chromatography ◽  
Fruit Peel ◽  
Dragon Fruit ◽  
Vacuum Liquid Chromatography ◽  
Layer Chromatography ◽  
Hylocereus Polyrhizus

  Objective: Indonesia is a country which has various natural resources including medicinal plants. Among the plants, red dragon fruit is interesting to be studied. In this study, antioxidant activity of red dragon fruit peel (Hylocereus polyrhizus (F.A.C. Weber) Britton and Rose) isolate was tested using 2,2-diphenyl-1-picrylhydrazyl method.Methods: Isolation of active compounds was performed through the application of vacuum liquid chromatography and preparative thin-layer chromatography (TLC). Antioxidant activity was tested using both TLC and spectrophotometry.Result: Results revealed that the values of IC50 of isolate 1 and 2 were 2.952, 14 μg/mL and 25.635,95 μg/mL, respectively.Conclusion: Isolate of dragon fruit has antioxidant activity. The compound which results in antioxidant activity is terpenoid and steroid.

scholarly journals Skrining Fitokimia Dan Analisis Kromatografi Lapis Tipis Ekstrak Etanol Kulit Buah Naga Merah (Hylocereus polyrhizus)

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Elisabeth Oriana Jawa La ◽  
Repining Tiyas Sawiji ◽  
Agustina Nila Yuliawati
Keyword(s):  
Secondary Metabolites ◽  
Thin Layer ◽  
Preliminary Test ◽  
Phytochemical Screening ◽  
Medicinal Products ◽  
Fruit Peel ◽  
Dragon Fruit ◽  
Layer Chromatography ◽  
Positive Results ◽  
Hylocereus Polyrhizus

Buah naga merah (Hylocereus polyrhizus) merupakan tumbuhan yang sudah banyak dimanfaatkan oleh masyarakat Indonesia secara alami sebagai tanaman kaya antioksidan, sebaliknya pemanfaatan dari kulit buah naga merah belum sepenuhnya dimaksimalkan. Beberapa penelitian yang telah dilakukan menunjukkan bahwa selain daging buah, kulit buah naga merah dapat dimanfaatkan sebagai sumber untuk pengobatan karena kaya akan antioksidan. Hal tersebut dapat digunakan sebagai dasar dan pilihan untuk menghasilkan produk obat tradisional dari kulit buah naga merah. Penelitian ini bertujuan untuk mengidentifikasi kandungan metabolit sekunder yang terdapat pada kulit buah naga merah berupa skrining fitokimia dan kromatografi lapis tipis (KLT) pada senyawa aktif yang disinyalir terkandung didalam kulit buah naga merah yang dapat dimanfaatkan dalam produk kefarmasian. Ekstraksi dilakukan dengan cara maserasi menggunakan pelarut etanol 96% diperoleh rendemen ekstrak sebanyak 2,96%. Skrining fitokimia dilakukan sebagai uji pendahuluan untuk mengidentifikasi kandungan metabolit sekunder yang terkandung dalam ekstrak kulit buah naga merah, dilanjutkan dengan KLT untuk mempertegas hasil reaksi positif. Hasil skrining dan KLT diperoleh kulit buah naga merah positif mengandung alkaloid, tanin, flavonoid, steroid dan potensial untuk dijadikan obat tradisional.Kata kunci :kulit buah naga merah, skrining fitokimia, KLT, metabolit sekunder Red dragon fruits (Hylocereus polyrhizus) generally has been widely known Indonesian as a plant that rich in antioxidant  however, the Red dragon fruits peel is not fully utilized. Some of studies have been conducted indicating that as well as its fruit flesh, Red dragon fruits peel can be beneficial as a source for treatment and traditional medicinal products since it is also rich of antioxidants.This research aims to identify the content of secondary metabolites on the Red dragon fruits peel  in the form of phytochemical screening and thin-layer chromatography (TLC) in the active compounds which can be utilized in the product of  Pharmacy. Extraction is conducted by maceration using the 96% ethanol solvent in order to produces extracts as much as 2.96 %.  Phytochemical screening is delivered as a preliminary test to identify the content of secondary metabolites contained in the peel of  Red dragon fruit peel and continued with the TLC to confirmed give positive  results. Results of  the screening and TLC obtained that the peel of  Red dragon fruits positive contains alkaloids, tannins, flavonoids, steroids and potential to be used as traditional medicineKeyword : Red dragon fruit peel, phytochemical screening, TLC, secondary metabolites 

scholarly journals Fractionation of Garcinia Mangostana Fruit Pericarp Cellulase Assisted Extracts by Preparative Thin Layer Chromatography and High Performance Liquid Chromatography

2020 ◽  
Vol 18 (1) ◽  
pp. 31
Author(s):  
Titania Tjandrawati Nugroho ◽  
Krisna Puja ◽  
Yum Eryanti ◽  
Miranti Miranti
Keyword(s):  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
High Performance ◽  
Preparative Thin Layer Chromatography ◽  
Garcinia Mangostana ◽  
Fruit Pericarp ◽  
Layer Chromatography

The polar extract of Mangosteen (Garcinia mangostana) fruit pericarp obtained by cellulase assisted ethanol extraction has strong antioxidant activity, giving an average 2,2 diphenyl-1 pykrylhydrazyl (DPPH) radical  scavenging IC50 of 13.9 µg/mL. In order to elucidate the chemical component from this extract that is responsible for the high antioxidant activity, fractionation of the extract should firstly be performed. In this paper we show results of preparative fractionation of the polar extract by two methods, namely preparative Thin Layer Chromatography (PTLC) and preparative High Performance Liquid Chromatography (PHPLC). PTLC used Silica Gel G60 plates, with a hexane:ethyl acetate (6:4) eluent. PHPLC was a reverse phase method, using C18 column and water:acetonitrile gradient elution. 4 fractions from PTLC and 6 fractions from PHPLC were collected and their antioxidant activity analyzed. Both methods gave separated fractions with lower antioxidant activity than the unfractionated original crude extracts, showing that the strong antioxidant activity of Mangosteen pericarp polar extracts maybe due to the concerted synergetic effect of several compounds, rather than a single isolated compound. It also shows the high degree of difficulty in separating mangosteen pericarp polar components having antioxidant activity for further structural analysis.

scholarly journals Formulation, Physical Quality Evaluation, and Antioxidant Activity of Body Butter of Ethanol Extract of Dragon Fruit (Hylocereus polyrhizus) Peel

2020 ◽  
Vol 25 (3) ◽  
Author(s):  
Agustina Nila Yuliawati ◽  
Kadek Duwi Cahyadi
Keyword(s):  
Antioxidant Activity ◽  
Quality Evaluation ◽  
Activity Index ◽  
Ethanol Extract ◽  
The Body ◽  
Fruit Peel ◽  
Physical Quality ◽  
Antioxidant Content ◽  
Dragon Fruit ◽  
Hylocereus Polyrhizus

Dragon fruit (Hylocereus polyrhizus) has polyphenols as an antioxidant. It has known that the antioxidant content of dragon fruit peels was more than in the flesh, so it can be used as a source of natural antioxidants to replace synthetic antioxidants. The use of dragon fruit (Hylocereus polyrhizus) peel, especially as topical preparations in the form of body butter, was still rarely done, whereas dragon fruit peel as an antioxidant can be used as an active ingredient of cosmetics. The purpose of this study was to obtain the body butter formula of ethanol extract of dragon fruit (Hylocereus polyrhizus) peel and its physical quality evaluation, to know the antioxidant activity of ethanol extract of dragon fruit (Hylocereus polyrhizus) peel and its body butter. This research was an experimental study with the stages of research consisting of determination of native plants, making ethanol extract of dragon fruit (Hylocereus polyrhizus) peel, ensuring its activity antioxidant, performing body butter formulation procedures, carrying out physical quality evaluation such as organoleptic, homogeneity, pH, spread, protection, and adhesion ability, then antioxidant activity of its body butter. The result of this research showed that the ethanol extract of dragon fruit (Hylocereus polyrhizus) peel has a moderate level of antioxidant (Antioxidant Activity Index / AAI = 0,88). Furthermore, body butter which has contains antioxidant content of ethanol extract of dragon fruit (Hylocereus polyrhizus) peel as much as 0.5% has the best physical quality evaluation during storage and the highest AAI (0,54) among other body butter formulas.

scholarly journals Recent Trends in the Application of Chromatographic Techniques in the Analysis of Luteolin and Its Derivatives

Biomolecules ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 731 ◽  
Author(s):  
Juszczak ◽  
Zovko-Končić ◽  
Tomczyk
Keyword(s):  
Liquid Chromatography ◽  
Medicinal Plants ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Analytical Methods ◽  
High Performance ◽  
Biological Effects ◽  
Immunomodulatory Activity ◽  
Layer Chromatography

Luteolin is a flavonoid often found in various medicinal plants that exhibits multiple biological effects such as antioxidant, anti-inflammatory and immunomodulatory activity. Commercially available medicinal plants and their preparations containing luteolin are often used in the treatment of hypertension, inflammatory diseases, and even cancer. However, to establish the quality of such preparations, appropriate analytical methods should be used. Therefore, the present paper provides the first comprehensive review of the current analytical methods that were developed and validated for the quantitative determination of luteolin and its C- and O-derivatives including orientin, isoorientin, luteolin 7-O-glucoside and others. It provides a systematic overview of chromatographic analytical techniques including thin layer chromatography (TLC), high performance thin layer chromatography (HPTLC), liquid chromatography (LC), high performance liquid chromatography (HPLC), gas chromatography (GC) and counter-current chromatography (CCC), as well as the conditions used in the determination of luteolin and its derivatives in plant material.

scholarly journals PENGARUH SUHU PENCELUPAN TERHADAP KARAKTERISTIK MINUMAN TEH HERBAL KULIT BUAH NAGA MERAH (Hylocereus polyrhizus)

2019 ◽  
Vol 8 (1) ◽  
pp. 18
Author(s):  
I Komang Ardianta ◽  
Ni Made Yusa ◽  
I Nengah Kencana Putra
Keyword(s):  
Antioxidant Activity ◽  
Temperature Treatment ◽  
Total Phenol ◽  
Herbal Tea ◽  
Fruit Peel ◽  
Red Color ◽  
Dragon Fruit ◽  
Dipping Temperature ◽  
Juice Content ◽  
Hylocereus Polyrhizus

This research aimed to find out effect of the dipping water temperature on characteristics of  herbal tea drink from red dragon fruit peel. This study used the completely random design of treatment with dipping temperature treatment 50oC, 60oC, 70oC, 80oC, 90oC and 100oC. The treatment was repeated in 3 replications, resulting in 18 experimental units. Data were analyzed by variance analysis and continued with Duncan's test. The results showed that the dipping temperature of herbal tea drink from red dragon fruit peel affected the total phenol, antioxidant activity, color taste and overall appearance. The best treatment was obtained with temperature of 100oC which produced herbal tea drinks with antioxidant activity (IC50) which was 8.50 mg/ml, juice content 1.94%, total phenol 0.83 g/100g, and sensory characteristics of color was very red color and liked, ordinary aroma, taste liked and overall acceptance liked.

scholarly journals OPTIMASI PENGOLAHAN TEH KULIT BUAH NAGA (Hylocereus polyrhizus)

2016 ◽  
Vol 3 (1) ◽  
Author(s):  
Mukhti Ali
Keyword(s):  
Antioxidant Activity ◽  
Significant Influence ◽  
Drying Method ◽  
Direct Sunlight ◽  
Fruit Peel ◽  
Significance Level ◽  
Fruit Skin ◽  
Dragon Fruit ◽  
Drying Techniques ◽  
Hylocereus Polyrhizus

Dragon fruit skin that had not been processed as waste in an optimal, even just be a waste to society, but the skin can be processed into a dragon fruit dragon fruit peel tea. The purpose of this study was to determine the levels of antioxidants and antioxidant activity, as well as the preference level of the tea panelists red dragon fruit peel and white by using the oven drying method, UV plastic, and direct sunlight. The study also aimed to analyze variations in the type of dragon fruit and drying techniques to the levels of antioxidants and antioxidant activity, as well as the properties of tea organoliptik dragon fruit skin. In this study used six combinations between types of dragon fruit (Red and White) and the drying method (Oven, Plastic UV and Direct Sunlight. The parameters analyzed were the levels of antioxidants and antioxidant activity and the level of A panelist on color, flavor, and aroma.The results show that there is significant influence on the kind of dragon fruit antioxidant levels and antioxidant activity at the 5% significance level. While the drying method does not give a different effect on the levels of antioxidants and antioxidant activity. The next type of dragon fruit also gives different effect on the preference level of the color of tea panelists dragon fruit skin, while the drying method does not provide a real impact. However, the type of dragon fruit no significant effect on the taste and aroma of tea dragon fruit skin. As well as the drying method, no significant effect on the taste and aroma of tea dragon fruit skin.  Keywords: dragon fruit skin, drying, tea, antioxidant levels, antioxidant activity. 

scholarly journals Antioxidant Activity of Daemonorops draco Resin

2019 ◽  
Vol 22 (5) ◽  
pp. 179-183 ◽  
Author(s):  
Sri Purwanti ◽  
Wulan Tri Wahyuni ◽  
Irmanida Batubara
Keyword(s):  
Antioxidant Activity ◽  
Infrared Spectroscopy ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Preparative Thin Layer Chromatography ◽  
Layer Chromatography

Jernang resin is secretion of jernang rattan (Daemonorops draco, Arecaceae family) fruits which is endemic in Southeast Asia. This resin has various biological activities and empirically used as wound healing, headache medicines, and fever remedies by Anak Dalam ethnic group from Jambi. This study was performed to evaluate the antioxidant activity of nonpolar fraction of D. draco resin which collected from Jambi Province, Sumatera, Indonesia. Resin was extracted with n-hexane, ethyl acetate, and methanol respectively. The antioxidant properties of the extracts were then evaluated using 1,1-diphenyl-2picryl-hidrazyl radical scavenging assay. The most active extract was further fractionated using n-hexane and methanol and separated using column chromatography and preparative thin layer chromatography. Separation of the extract was conducted through antioxidant assay-guided fractionation. Characterization of the active fraction was carried out by infrared spectroscopy. The result shows that ethyl acetate extract provides higher antioxidant activity (IC50 = 27.61 µg/mL) compare to methanol and n-hexane extracts. N-hexane fraction of ethyl acetate extract used for further separation using column and preparative thin layer chromatography due to its antioxidant activity. Separation using column chromatography resulting in 9 fractions (F.1-9). Fraction F.5 provide high antioxidant activity (IC50 = 17.27 µg/mL) and further separated using preparative thin layer chromatography resulting two fractions with lower antioxidant activity F.5.1 (IC50 = 85.18 µg/mL) and F.5.2 (IC50 = 34.94 µg/mL). Characterization of fraction F.5.2 using infrared spectroscopy showed that component in fraction F.5.2 contains NH-substituted benzene.

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