The Effects of Topical Vitamin C on Burn Wound Healing

Background and Aim: Topical supplements and vitamins have often been used to accelerate the healing of burn wounds. This study investigated the effects of topical application of vitamin C solution on burn wounds. Methods & Materials: This research was performed on 30 patients with extensive second-degree burns on one or two organs with similar burns. The study subjects were randomly divided into the intervention and control groups. The intervention group received topical application of vitamin C solution in addition to silver sulfadiazine ointment in the burn area; however, only silver sulfadiazine ointment was applied in the burn area in the control group. The wounds were examined and scored on days 1, 3, 7, and 14 using the Bates-Jensen Wound Assessment Tool. The collected data were analyzed in SPSS using repeated-measures Analysis of Variance (ANOVA). Ethical Considerations: This study was approved by the ethics committee of sabzevar university medical sciences. (Code: IR.MEDSAB.REC.1394.60). Results Eighteen of the 30 examined patients were male and 12 were female. Their Mean±SD age and body mass index were 43.03±11.90 years and 27.82±4.08 kg/m2, respectively. Conclusion: The repeated-measures ANOVA data indicated that the study groups were significantly different concerning the final mean scores given to the wounds (P=0.047). In other words, the research groups significantly differed in wound healing; thus, topical application of vitamin C significantly improved wound healing in the study participants.

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Preparation and evaluation of burns wound healing ointment base of leaves and stem bark of Anthocleista djalonensis (L) extract using animal model.

INTERNATIONAL JOURNAL OF PHARMACEUTICAL EDUCATION AND RESEARCH (IJPER) ◽

10.37021/ijper.v1i2.1 ◽

2020 ◽

Vol 1 (02) ◽

pp. 25-32

Author(s):

Chinenye Nnenna Ugwu ◽

Ezinwanne Nneoma Ezeibe ◽

Chinekwu Sherridan Nwobi ◽

Chinelo Charity Eze ◽

Somtochukwu Amuche Evurani ◽

...

Keyword(s):

Wound Healing ◽

Stem Bark ◽

Positive Control ◽

Silver Sulfadiazine ◽

Wound Contraction ◽

Bark Extract ◽

Control Group ◽

Burn Wound ◽

Burn Wounds ◽

Ointment Base

BACKGROUND: Burn wounds are inescapable life events especially in low income areas. Contamination of the burn wound site results in localized wound infection, hence the need for potent phytochemicals readily available for wound healing. The use of stem bark of Anthocleista djalonensis efficacy for burn wound has not been evaluated to the best of our knowledge. AIM: The study is aimed at evaluating and comparing the wound healing potential of ointment base of leaves and Stem bark of Anthocleista djalonensis on burn wounds created on wistar albino rats. METHODS: The leaves and Stem bark of Anthocleista djalonensis collected were extracted using 95%v/v methanol and phytochemical analysis conducted. Simple ointments of varying concentrations were formulated to screen for wound healing activity using the burn wound model on experimental rats grouped into six (n=4). Group 1 was treated with silver sulfadiazine cream (positive control), group 2 with ointment base (negative control), group 3 with 1% stem bark extract ointment, group 4 with 2% stem bark ointment, group 5 with 1% leave extract ointment, and group 6 with 2% leave extract ointment. All animals were anesthetized before the creation of burn wounds. Measurement was taken on day zero and the wound was left untreated for 48 hours in order to allow bacterial colonization before daily treatment of the wound for 16 days. RESULTS: The result of the phytochemical screening revealed that both extract of Anthocleista djalonensis contains flavonoids, tannins and saponins. On day 2, 1% stem bark, 2% stem bark and 1% leaf extract had 16%, 15%, and 10% wound contraction respectively which was higher than the 8.5% wound contraction of silver sulfadiazine. Also as the concentration of the extract increased, the wound healing effect also increased as seen by the percentage wound contraction on day 16 for all treatment. CONCLUSION: The findings of the study have shown that methanolic extracts of stem bark and leaf of Anthocleista djalonensis contained bioactive constituents which have burn wound healing activity. The stem bark extract showed better activity when compared with the leaf extract and also the positive control (silver sulfadiazine).

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Evaluation of the effects of Vitamin C on Epithelialization of Burn Wounds: A Randomized Clinical Trial

10.21203/rs.3.rs-28024/v2 ◽

2020 ◽

Author(s):

Mohammd Haddadi ◽

Elaheh Jaghouri ◽

Davood Robat Sarpooshi ◽

Fateme Ghobadi ◽

Hamid Robat Sarpooshi

Keyword(s):

Clinical Trial ◽

Vitamin C ◽

Repeated Measures ◽

Assessment Tool ◽

Burn Wound ◽

Repeated Measures Anova ◽

Burn Wounds ◽

Significant Difference ◽

Treatment Data ◽

Second Degree

Abstract Objectives The purpose of this study is to determine the effect of topical vitamin C on second-degree burn wounds restoration. This was a clinical trial. The sample size was determined as 30. Participants were selected from patients suffering from second-degree burns who visited the Burn Center of Vaseei Hospital in Sabzevar. Both intervention and control groups targeted one patient.Dressing was changed in a daily manner after cleansing. A topical vitamin C solution was administered on the wounds. The Bates-Jensen Wound Assessment Tool was used to evaluate burn wound parameters in the 1 st , 3 rd , 7 th , and 14 th days of treatment. Data was analyzed using SPSS v.16 and ‘’repeated measures ANOVA. Results The average age of participants was 43.33 ± 11.9 years and 60% of the participants were males and 40% of them were females. The results of repeated measures ANOVA showed a statistically significant difference in mean scores of the wound between the two treatments (P = 0.047), wound healing significantly differed in the two groups and topical vitamin C solution had a significant effect on the acceleration of wound Epithelialization. The administration of topical vitamin C is recommended for epithelialization of second-degree burns.

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Topical treatment of standardised burns with herbal remedies in model rats

Bosnian Journal of Basic Medical Sciences ◽

10.17305/bjbms.2005.3233 ◽

2005 ◽

Vol 5 (4) ◽

pp. 50-57 ◽

Cited By ~ 1

Author(s):

Fahir Bečić ◽

Nedžad Mulabegović ◽

Zakira Mornjaković ◽

Elvedina Kapić ◽

Senad Prašović ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Silver Sulfadiazine ◽

Herbal Remedies ◽

Control Group ◽

Therapeutic Effects ◽

Skin Area ◽

Burn Wound ◽

Burn Wounds ◽

Burn Wound Healing

Experimental studies of burns require the use of different animal models. The aim of this work was to establish experimental model of thermal injuries and to evaluate the effects of topical agents on healing of the burn wounds. Forty female Wistar rats were randomly classified in 4 groups and isolated for 2 weeks before the onset of experiment. Animals were primarily anaesthetized with pentobarbital-sodium and then shaved (skin area of their back with diameters 5 cm x 5 cm). A round metal stamp with contact area of 5 cm2 and total weight of 100 g was heated up to 80°C and then applied without additional pressure on the depilated skin of the back for 14 seconds. This procedure produced a standardized burn wound. Induced burn wounds were immediately drowned in the 4°C-water for 3 s in order to maintain microcirculation. After the inducement of thermal injures, all rats were treated with 1% silver sulfadiazine cream, herbal topical preparations or were not treated at all. Burn wounds were treated twice a day until the healing completion. The result of treatment application was a significant reduction of burn wound diameters. Herbal topical preparations expressed positive therapeutic effects on the parameters of burn wounds. The efficiency of silver sulfadiazine cream in burn wound healing was significantly more expressed in comparison to healing process in control group of animals (p<0,001). We conclude that herbal topical preparations efficiently caused the completion of burn wound healing process without scar formation.

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MANFAAT EKSTRAK ETANOL DAUN REMEK DAGING (Hemigraphis colorata W. Bull) TERHADAP LUKA BAKAR PADA TIKUS

Jurnal Tumbuhan Obat Indonesia ◽

10.22435/jtoi.v13i1.2823 ◽

2020 ◽

Vol 13 (1) ◽

pp. 32-41

Author(s):

Lusi Putri Dwita ◽

Vera Ladeska ◽

Aisyah Ramadhani ◽

Dwi Rahma Augusta ◽

Retno Tri Saufia

Keyword(s):

Wound Healing ◽

Topical Application ◽

Ethanolic Extract ◽

Positive Control ◽

Negative Control ◽

Silver Sulfadiazine ◽

Burn Wound ◽

Burn Wounds ◽

Wound Induction ◽

Collagen Density

ABSTRACT Remek daging (Hemigraphis colorata W.Bull) have been studied and used traditionally for wound healing. This study aimed to determine the effect of topical application of remek daging leaves ethanolic extract 70% on the burn wound. The animals used for this study were 30 rats, divided into five groups, namely 20, 10, 5% remek daging extract ointment, negative control (vaseline flavum), and positive control (silver sulfadiazine 1%). Histology observations were held on days 3, 7, and 14 after burn wound induction. Histological observations showed an increase number of macrophages, fibroblasts, collagen density, and re-epithelialization in the extract ointment group significantly compare to the negative control (p <0.05). The application of ointment extract 20% to the rats showed comparable results to silver sulfadiazine 1% (p> 0.05). It can be concluded that remek daging ointment extract can accelerate the healing of burn wounds with the best results at a concentration of 20%. Keywords: Hemigraphis colorata, burns, macrophages, fibroblasts, collagen. ABSTRAK Remek daging (Hemigraphis colorata W.Bull) telah diteliti dan digunakan untuk penyembuhan luka secara tradisional. Penelitian ini bertujuan untuk mengetahui pengaruh ekstrak etanol 70% daun remek daging secara topikal pada luka bakar tikus putih. Hewan yang digunakan adalah 30 ekor tikus yang dibagi menjadi 5 kelompok yaitu kelompok salep ekstrak daun remek daging 20, 10, 5% (%b/b), kontrol negatif (vaselin flavum) dan kontrol positif (silver sulfadiazine 1% (%b/b)). Pengamatan secara histologi dilakukan pada hari ke 3, 7 dan 14 setelah induksi luka bakar. Pengamatan histologi menunjukkan peningkatan jumlah makrofag, jumlah fibroblas, kepadatan kolagen dan ketebalan re-epitelisasi pada kelompok salep ekstrak daun remek daging secara signifikan dibandingkan kontrol negatif (p<0,05). Tikus yang diberikan perlakuan salep ekstrak 20% menunjukkan hasil sebanding dengan silver sulfadiazin 1% (p>0,05). Dapat disimpulkan bahwa salep ekstrak daun remek daging dapat mempercepat penyembuhan luka bakar dengan hasil terbaik pada konsentrasi 20%. Kata kunci: Hemigraphis colorata, luka bakar, makrofag, fibroblas, kolagen.

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Evaluation of The Effects of Vitamin C on Epithelialization of Burn Wounds

10.21203/rs.3.rs-28024/v1 ◽

2020 ◽

Author(s):

Mohammd Haddadi ◽

Elaheh Jaghouri ◽

Davood Robat Sarpooshi ◽

Fateme Ghobadi ◽

Hamid Robat Sarpooshi

Keyword(s):

Vitamin C ◽

Repeated Measures ◽

Assessment Tool ◽

Burn Wound ◽

Repeated Measures Anova ◽

Burn Wounds ◽

Degree Burn ◽

Significant Difference ◽

Treatment Data ◽

Second Degree

Abstract Objectives: The purpose of the present study is to determine the effect of topical vitamin C on second degree burn wounds restoration. This was a clinical trial. The sample size was determined as 30. The participants were selected from the patients suffering from second degree burns who visited the Burn Center of Vaseei Hospital in Sabzevar. Both intervention and control groups targeted one patient. Dressing was changed in a daily manner after cleansing. Topical vitamin C solution was administered on the wounds. The Bates-Jensen Wound Assessment Tool was used to evaluate burn wound parameters in the 1st, 3rd, 7th and 14th day of treatment. Data analysis was performed using SPSS v.16 and ‘’repeated measures ANOVA.Results: The average age of participants was 43.33 ± 11.9 years and 60% of the participants were males and 40% of them were females. The results of repeated measures ANOVA showed a statistically significant difference in mean scores of wound between the two treatments (P = 0.047), wound healing significantly differed in the two groups and topical vitamin C solution had a significant effect on acceleration of wound Epithelialization. According to the results of this study, administration of topical vitamin C is recommended for epithetlialization of second degree burns.IRCT: IRCT2015101224487N1

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673 Effect of Subcutaneous Topical Ozone Therapy on Second Degree Burn Wounds in Rats: An Experimental Study

Journal of Burn Care & Research ◽

10.1093/jbcr/irab032.319 ◽

2021 ◽

Vol 42 (Supplement_1) ◽

pp. S193-S193

Author(s):

Emre Karakaya ◽

Aydincan Akdur ◽

H Ebru ◽

Ayvazoglu Soy ◽

Alev Ok Atilgan ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Silver Sulfadiazine ◽

Male Rats ◽

Control Group ◽

Ozone Therapy ◽

Burn Wounds ◽

Degree Burn ◽

Significant Difference ◽

Second Degree

Abstract Introduction Burn is one of the most severe traumas that causes coagulative destruction of the skin. The use of various products that accelerate wound healing in patients with burn may affect the patient’s survival and reduce the complications that may be seen. In the present study we aimed effects of subcutaneous ozone injection on second degree burn wound. Methods A total of 72 Sprague-Dawley male rats included in the study were divided randomly into three groups (control group (CG), silver sulfadiazine group (SG), ozone group (OG)) and each group was divided randomly two subgroups (as sacrificed on d7 and on d14).A deep second degree scald burns were created on the lower back. In CG subcutaneous 0.9% serum saline was injected daily into the burn area. In SG, burns were dressed with silver sulfadiazine daily and in OG subcutaneous ozone was injected daily into the burn area. Tissue hydroxyproline level measurement and histopathological evaluation were done. Results When the groups were compared in terms of weight change, no significant difference was found on the 7th and 14th days. In the evaluation made in terms of tissue hydroxyproline, tissue hydroxyproline level in OG was found to be significantly higher on both the 7th and 14th days (p < 0.001). In histopathological evaluations, it was determined that wound healing in OG was significantly higher than in the other groups. Conclusions According to the results, subcutaneous ozone therapy is more effective than silver sulphadiazine in the healing process of second-degree burn wounds and it can be safely used in the treatment of burn wounds.

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EFFECT OF HEALOL OIL ON WOUND HEALING IN WISTAR RATS: A NOVEL STUDY

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i3.23223 ◽

2018 ◽

Vol 11 (3) ◽

pp. 152

Author(s):

Sujith V ◽

Poornima G ◽

Balaji O ◽

Bairy Kl ◽

Praveen K ◽

...

Keyword(s):

Wound Healing ◽

Wistar Rats ◽

Chronic Wounds ◽

Statistical Significance ◽

Silver Sulfadiazine ◽

Histopathological Analysis ◽

Burn Wound ◽

Excision Wound ◽

Burn Wounds ◽

Wound Model

Objective: Wound healing is a complex process, and various plant extracts have been used to study the effect of medicinal plants on wound healing. Healol oil is used in some tribal areas for ulcer healing and treatment of burn wounds. There are no animal studies done so far using healol oil to find out its role in the treatment of wounds. Hence, the aim of our study is to find the effect of healol oil in excision as well burn wounds in Wistar rats.Methods: Thirty-six Wistar rats were used, 18 rats in excision wound model and remaining in burn wound model. Each model consists of three groups of six rats each. Povidone-iodine was used as a standard control in excision wound model and silver sulfadiazine was used as a standard control in burn wound model. Healol oil was used as test drug in both the models. Period of epithelization and wound contracture rates were analyzed. Histopathological analysis of the skin tissue was done. One-way analysis of variance was used followed by Tukey’s post hoc test for statistical analysis using SPSS version 23 software, P value of <0.05 was taken as statistical significance.Results: Both standard and test groups showed significantly faster wound healing (p<0.001) compared to control rats treated with paraffin wax in both the models. In excision model standard povidone was significantly (p<0.001) better compared to healol oil whereas in burn model, silver sulfadiazine and healol oil showed comparable results with respect to period of epithelization without statistical significance (p>0.05).Conclusion: Wound healing property of healol oil is proved, and further clinical trial can be done to find out the effect of healol oil on chronic wounds due to various etiologies.

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Sufficient therapeutic effect of cryopreserved frozen adipose-derived regenerative cells on burn wounds

10.1101/436444 ◽

2018 ◽

Author(s):

Yasuhiko Kaita ◽

Takehiko Tarui ◽

Hideaki Yoshino ◽

Takeaki Matsuda ◽

Yoshihiro Yamaguchi ◽

...

Keyword(s):

Wound Healing ◽

Therapeutic Effect ◽

Healing Process ◽

Control Group ◽

Type I ◽

Burn Wound ◽

Burn Wounds ◽

Burn Wound Healing ◽

Significant Difference ◽

Regenerative Cells

AbstractThe purpose of this study was to evaluate whether cryopreserved (frozen) adipose-derived regenerative cells (ADRCs) have a therapeutic effect on burn wound healing as well as freshly isolated (fresh) ADRCs.Full thickness burns were created on dorsum of nude mice and burn wound was excised. The wound was covered by artificial dermis with; (i) fresh ADRCs, (ii) frozen ADRCs, and (iii) PBS (control). The assessment for wound healing was performed by morphological, histopathological and immunohistochemical analyses.In vivo analyses exhibited the significant therapeutic effect of frozen ADRCs on burn wound healing up to the similar or higher level of fresh ADRCs. There were significant differences of wound closure, epithelized tissue thickness, and neovascularization between the treatment groups and control group. Although there was no significant difference of therapeutic efficacy between fresh ADRC group and frozen ADRC group, frozen ADRCs improved burn wound healing process in dermal regeneration with increased great type I collagen synthesis compared with fresh ADRCs.These findings indicate that frozen ADRCs allow us to apply not only quickly but also for multiple times, and the cryopreserved ADRCs could therefore be useful for the treatment of burn wounds in clinical settings.

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Effects of extractum cepae, heparin, allantoin gel and silver sulfadiazine on burn wound healing: an experimental study in a rat model

Veterinární Medicína ◽

10.17221/6015-vetmed ◽

2012 ◽

Vol 57 (No. 6) ◽

pp. 287-292 ◽

Cited By ~ 7

Author(s):

AS Durmus ◽

M. Yaman ◽

HN Can

Keyword(s):

Wound Healing ◽

Rat Model ◽

Silver Sulfadiazine ◽

Control Group ◽

Albino Rats ◽

Burn Wound ◽

Tissue Samples ◽

Burned Areas ◽

Positive Effects ◽

Burn Wound Healing

These experiments were conducted in order to compare the effects of extractum cepae, heparin, allantoin gel (CTBX) and silver sulfadiazine (SSD) cream on burn wound healing in rats. Thirty six adult, female Wistar albino rats were divided into three equal groups. A burn was made on the back of all rats. The burned areas in the first, second and third groups were covered with cold cream (control), SSD skin cream and CTBX twice a day, respectively. Seven and 14 days later, the rats were sacrificed and burned skin tissue samples were collected from the rats for histopathological examinations. Histopatological evaluations on the 7<sup>th</sup> and 14<sup>th</sup> days showed burn healing to be better in the CTBX and SSD groups with respect to the control group. The best burn wound healing was observed in the CTBX group (P < 0.001). Wound healing was significantly different between the groups at days 7 and 14 (P < 0.001). In conclusion, application of CTBX has significant positive effects on the healing of burn wounds in a rat model.  

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Comparison Profile of Nutrition Status in Stage V Chronic Kidney Disease Patients Hemodialyzing Using New and Reuse Dialyzer

Journal of Agromedicine and Medical Sciences ◽

10.19184/ams.v5i2.9613 ◽

2019 ◽

Vol 5 (2) ◽

pp. 17

Author(s):

Yuli Hermansyah ◽

Ika Aulia Kurniasari ◽

Heni Fatmawati

Keyword(s):

Oxidative Stress ◽

Wound Healing ◽

Antioxidant Properties ◽

Negative Control ◽

Silver Sulfadiazine ◽

Control Group ◽

Burn Wound ◽

Burn Wound Healing ◽

Degree Burn ◽

Significant Difference

Burn wound healing process consists of three phases of inflammation, proliferation and remodelling. The number of fibroblasts has increased during the proliferation phase. Oxidative stress in burn may disrupt proliferation through TGF-β inhibition, result in decreased number of fibroblasts and poor burn wound healing. Genistein in edamame seeds has anti-inflammatory, antibacterial and antioxidant properties. Edamame contains vitamins A, C, and E which is also have antioxidant properties. Edamame has been suggested to protect burn wound healing from oxidative stress. This study aimed to understand the effectiveness of edamame seed (Glycine max L. Merril) ethanolic extract (ED) to fibroblast count in second degree burn wound healing. This is true experimental research with post test only control group design. The sample of this research was 24 rat models of second degree burn which was made by attaching hot metal (60 ° C) for 5 seconds on the shaved back of the rats. The sample was divided into 6 groups with topical treatment, among others: positive control group (silver sulfadiazine), negative control group (Na CMC 0.5%), group with ED 20%, ED 40%, ED 60%, and ED 80%. Treatment was given for 15 days and skin sample was taken on the 16th day. Observation of the number of fibroblasts was done by taking pictures of histhologic preparations in Hematoxylin and Eosin staining under 400x magnification of light microscope. In one way ANOVA test, significance value of 0.011 (p≤0,05) indicated that there was at least a significant difference between 2 groups. LSD’s post hoc analysis showed that there was a significant difference in the group ED 20, 40, and 60% compared with negative control. Keyword: burn wound healing, vegetable soybean, genistein, fibroblast count, silver sulfadiazine

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