Effect of a high monounsaturated fatty acid diet alone or with combined vitamin E and C, or lycopene intake on oxidative stress in patients with type 2 diabetes mellitus

2012 ◽  
Vol 12 (2) ◽  
pp. 81-86
Author(s):  
Maha Badkook ◽  
Fiona McCullough ◽  
Nessar Ahmed
Keyword(s):  
Oxidative Stress ◽  
Type 2 Diabetes ◽  
Fatty Acid ◽  
Vitamin E ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Total Antioxidant ◽  
Mufa Diet ◽  
Vitamin E And C

The aims of this study were to investigate the effect of a high monounsaturated fatty acid (MUFA) diet alone or with combined vitamin E and C, or lycopene intake on oxidative stress in type 2 diabetes in Saudi Arabia. Forty-eight type 2 diabetic patients consumed a high MUFA diet for 16 weeks. After four weeks of high MUFA diet alone, supplements of vitamins E (400 mg) and C (1,000 mg) were taken for four weeks, followed by a four-week washout period. In the final four weeks, subjects consumed a high MUFA diet with tomato paste (equivalent to 12 mg lycopene). Plasma samples were tested for vitamin E and C, lycopene, malondialdehyde (MDA), total antioxidant status, fasting plasma glucose and glycated haemoglobin A1c (HbA1c). A high-MUFA diet with vitamins E and C or lycopene caused significant elevation of plasma vitamins E, C and lycopene compared to a high-MUFA diet alone. Plasma MDA was reduced with vitamins, but not lycopene supplementation. The total antioxidant status increased significantly following a high-MUFA diet and with vitamin and lycopene supplementations. Fasting glucose was not affected whereas HbA1c decreased significantly after vitamin supplementation compared to baseline. A high-MUFA diet supplemented with vitamin E and C, or lycopene improves antioxidant status in type 2 diabetes.

scholarly journals ASSESSMENT OF DNA STRAND BREAKS AND TOTAL ANTIOXIDANT STATUS IN TYPE 2 DIABETIC PATIENTS WITH AND WITHOUT COMPLICATIONS- A CASE-CONTROL STUDY

2017 ◽  
Vol 10 (4) ◽  
pp. 430
Author(s):  
K Nithya ◽  
Isabel W ◽  
Angeline T ◽  
Priscilla As ◽  
Asirvatham Aj
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Dna Damage ◽  
Type 2 Diabetes Mellitus ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Diabetic Patients ◽  
Total Antioxidant

 Objective: To evaluate the total antioxidant status (TAS) and the extent of DNA strand breaks (damage) as a measure of oxidative stress biomarkers in Type 2 diabetic patients (with and without complications) and controls.Materials and Methods: Blood samples were collected from 200 patients with type 2 diabetes mellitus (n=100 with complications and n=100 without complications) and 100 healthy individuals. Oxidative DNA damage was evaluated using alkaline single cell gel electrophoresis (comet assay). Total antioxidant status was assessed by Ferric Reducing Ability of Plasma (FRAP) assay.Results: TAS was found to be significantly lower in type 2 diabetic patients (with and without complications) compared to controls (p< 0.001).  Similarly, patients with complications of type 2 diabetes mellitus had significantly lower TAS when compared to diabetic patients (p= 0.007). DNA damage analysis showed that the extent of damage was high in patients with diabetes mellitus (with and without complications) compared to controls (p< 0.001). Fasting glucose and glycosylated haemoglobin level (HbA1c) was found to be significantly higherin diabetic patients than controls (p< 0.05). Correlation analysis showed that there is no association between age, duration, sugar level, HbA1c, TAS and DNA damage in patients with Type 2 diabetes mellitus.Conclusion: Alterations in TAS and the extent of DNA damage was observed in patients with complications of diabetes mellitus indicate that oxidative stress is more in patients with complications when compared to patients without complications and healthy individuals. Therefore, further DNA damage and onset of complications in Type 2 diabetes mellitus could be prevented by counteracting the oxidative stress by therapeutic interventions using appropriate antioxidants.Key words: Hyperglycemia, Oxidative stress, DNA damage, Total antioxidant status, Type 2 diabetes mellitus, Vascular complication

scholarly journals A study of the Oxidative stress and Antioxidant status in diabetic subjects who are on treatment with Metformin

2019 ◽  
pp. 14-18
Author(s):  
Manju Koshy ◽  
Palocaren Jeeji ◽  
Sethupathy S
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
South Indian Population ◽  
Group I ◽  
Total Antioxidant ◽  
Group 2 ◽  
Type 2 Diabetic ◽  
Group 1

Background: Oxidative stress plays an important role in the pathogenesis of DM and its complications. However, antioxidant status and its contribution to type 2 DM are less explored in South Indian population. Metformin, is a biguanide anti hyperglycemic agent used for the management of type 2 diabetes. Aim:  To study the alteration in oxidant and antioxidant status in type 2 diabetic subjects on treatment with Metformin and to evaluate the effect of metformin in improving the total antioxidant status. Methodology: In this cross sectional study, all subjects were T2DM patients, on metformin monotherapy (500 mg, bd) and were grouped into two - Group 1 and Group 2 for the study purpose, based on their HbA1c values. Baseline parameters (B.P, Waist Hip ratio, BMI, family history), glycemic status, lipid profile, FRAP, TBARS and serum Metformin levels were assayed. Fasting and postprandial blood specimens were collected and plasma glucose concentrations were measured by standard methods. Fasting plasma total antioxidant capacity (TAC) was measured by ferric reducing ability of plasma (FRAP) assay. Oxidative stress was evaluated and measured as TBARS and the values were compared among the two groups. Results: TBARS levels were higher and FRAP levels were significantly lower in Group I subjects compared to Group II subjects and can be explained due to increased superoxide ions and reduced activity of S. O. D. Conclusion: It may be concluded that total antioxidant status is lower in type 2 diabetic subjects of Group 1 category compared to diabetic subjects in the Group 2 and it may be related to the beneficial effects of the biguanide, Metformin.

scholarly journals Lipid Peroxidation and Antioxidant Status in Male and Female Patients with Type 1 and Type 2 Diabetes Mellitus

Med Phoenix ◽  
2017 ◽  
Vol 1 (1) ◽  
pp. 10-14
Author(s):  
Nirjala Laxmi Madhikarmi ◽  
Prem Prakash Singh ◽  
Tarannum Khatun
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Total Antioxidant Activity ◽  
Male And Female ◽  
Total Antioxidant

Background: Free radicals are reactive oxygen species which cause lipid peroxidation precipitating many metabolic diseases including Diabetes Mellitus. However, these free radicals are quenched by substances known as antioxidants like vitamin C, vitamin E and several other compounds. Lipid peroxidation and antioxidant status were investigated in patients with Type 1 and Type 2 Diabetes mellitus- Pokhara, Nepal.Methods: The extent of lipid peroxidation was assessed by thiobarbituric acid reactive substances and the antioxidant parameter estimations were total antioxidant activity, Vitamin C and Vitamin E assessed in Type 1 and 2 diabetes mellitus patients along with matched healthy counterparts.Results: The lipid peroxidation was increased in male Type 1 and 2 diabetic patients whereas female group showed decreased level as compared to its healthy counterparts. Similarly, the total antioxidant activity was found to be decreased in the diabetic group. The lipid peroxidation parameter and antioxidant status were statistically significant at p< 0.05.Conclusion: Oxidative stress and antioxidant status varied in male and female patients suffering from diabetes either Type 1 or Type 2. Apart from gender basis of evaluating oxidative stress, variables based on diet, habitat, socioeconomic status, education, etc. can also be considered.MED Phoenix Volume (1), Issue (1) July 2016, page: 10-14

scholarly journals Total antioxidant status in type 2 diabetes mellitus with diabetic nephropathy

2019 ◽  
Vol 6 (3) ◽  
pp. 673
Author(s):  
Heena Singla ◽  
Gitanjali Goyal ◽  
Cheenu Garg ◽  
Kajal Bhalla
Keyword(s):  
Oxidative Stress ◽  
Diabetes Mellitus ◽  
Diabetic Nephropathy ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Total Antioxidant ◽  
Type 2 Diabetic

Background: Diabetes mellitus has emerged as one of the most common health hazard all over the world. Diabetic nephropathy is the most challenging long term complication of Type 2 Diabetes mellitus and microalbuminuria is the earliest marker of diabetic nephropathy. In diabetes, chronic hyperglycemia and deranged lipid profile lead to excess generation of free radicals. The increased oxidative stress plays a major role in pathogenesis of diabetic complications, including diabetic nephropathy. There are many naturally occurring antioxidant enzymes in our body. Diabetes has multiple effects on protein levels and activity of these antioxidant enzymes. This further augments the oxidative stress. There are many non-enzymatic antioxidants in our body which include vitamins A, C, E and trace minerals like copper, zinc, manganese and selenium.Methods: The study was done on a total of 150 subjects. Group A comprised of 60 Type 2 diabetic patients with diabetic nephropathy, Group B comprised of 60 Type 2 diabetic patients without diabetic nephropathy and Group C comprised 30 healthy controls. Total antioxidant status, microalbuminuria and glycosylated haemoglobin were measured.Results: In present study, authors found that total antioxidant status is drastically reduced in all diabetic patients, and it was found to be further low in patients with diabetic nephropathy. This decrease was found to be directly proportional to the degree of diabetic nephropathy, as measured by the levels of microalbuminuria.Conclusions: Timely institution of antioxidant supplementation therapy may emerge as a promising measure in delaying the onset and progression of diabetic complications, especially diabetic nephropathy.

scholarly journals Association of fibroblast growth factor 21 with oxidative stress and lipid profile in type 2 diabetes

2020 ◽  
Vol 8 (12) ◽  
pp. 4343
Author(s):  
Vineetha K. R. ◽  
Santha K. ◽  
Inmozhi R. ◽  
Periyasamy S. ◽  
Kanakasabai G. ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Growth Factor ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Fibroblast Growth Factor 21 ◽  
Diabetic Patients ◽  
Fibroblast Growth ◽  
Total Antioxidant

Background: Cardiovascular disease is the most prevalent cause of morbidity and mortality in diabetic patients. Fibroblast growth factor 21 (FBG 21) is an endocrine factor that regulates glucose and lipid metabolism, insulin resistance, and obesity. Blood levels of FGF21 are elevated in patients with atherosclerosis, macrovascular, and microvascular complications of diabetes, possibly due to a compensatory up regulation. Studies reported that FGF21 is an important regulator of mitochondrial and oxidative stress. The role of FGF21 in chronic diseases and the diminished oxidative stress observed with anti-diabetic therapy has been the target of new studies. Current study aimed to evaluate serum FGF21 levels and its association with oxidative stress and lipid profile levels in type 2 diabetic patients.Methods: 100 controls and 100 diabetic patients on oral hypoglycemic drugs between 35-55 years of age without any cardiac, renal, liver, and thyroid dysfunction were selected for this study. Oxidative stress (MDA), total antioxidant status (FRAP), and FGF21 were measured. FGF21 was analyzed by ELISA methods. Serum MDA was assessed by the method of Yagi  serum total antioxidant status was measured by the method of Benzie et al.Results: FGF21 level was increased in diabetic patients compared with controls. There was a significant positive correlation of FGF21 with MDA (r=0.875, p<0.01) and negative correlation with FRAP observed (r= -0.867 p<0.01). There was also positive correlation of FGF21 with total cholesterol (r=0.499, p<0.01), triglycerides (r=0.648, p<0.01), LDL-cholesterol (r=0.337, p<0.01) and negative correlation with HDL-cholesterol (r= -0.172, p<0.05) were observed.Conclusions: Increased oxidative stress and decreased antioxidant status were observed in diabetics. This could be due to dyslipidemia and increased generation of free radicals. High levels of FGF21 observed in our study might represent its resistant state and the compensatory response to maintain metabolic homeostasis. Further studies are needed to explore the role of FGF21 as a novel marker in predicting cardiovascular risk in diabetic patients.

Total antioxidant status before and after bariatric surgery for type 2 diabetes mellitus

Metabolism ◽  
2009 ◽  
Vol 58 (9) ◽  
pp. 1366 ◽  
Author(s):  
Sarah L. Prior ◽  
Stephen C. Bain ◽  
Jeffrey W. Stephens ◽  
Imran Alam ◽  
John N. Baxter
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Bariatric Surgery ◽  
Type 2 Diabetes Mellitus ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Before And After ◽  
Total Antioxidant

scholarly journals Oxidative Stress Status in Sera and Saliva of Type 2 Diabetic Iraqi Patients with and without Proliferative Diabetic Retinopathy

2019 ◽  
Vol 31 (3) ◽  
pp. 719-722
Author(s):  
Hathama Razooki Hasan ◽  
Nuha Nihad A. Aburahma ◽  
Abdul Kareem A. AL-Kazaz
Keyword(s):  
Oxidative Stress ◽  
Diabetic Retinopathy ◽  
Proliferative Diabetic Retinopathy ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Stress Index ◽  
Total Antioxidant ◽  
Oxidant Status ◽  
And Oxidative Stress

The present study aimed to look for the differences in the oxidative stress status in sera and saliva samples of type 2 diabetic Iraqi patients with and without proliferative diabetic retinopathy. As well as to look for the possibility whether this status can be measured in saliva as an alternative sample to that of serum, hence to achieve that total oxidant status, total antioxidant status and oxidative stress index were measured in both sera and saliva samples of two groups of patients with type 2 diabetes mellitus and the healthy individuals. Upon the comparison between patients without proliferative diabetic retinopathy and the control sample the results showed presence of a significant increase (p < 0.05) of total oxidant status and oxidative stress index in sera and saliva samples, while there was a significant decrease (p < 0.05) in total antioxidant status of sera and saliva samples. Meanwhile when the comparison was done between patients with proliferative diabetic retinopathy and those without proliferative diabetic retinopathy, a significant increase (p < 0.05) in both salivary total oxidant status and oxidative stress index was observed with a significant decrease (p < 0.05) in sera and salivary total antioxidant status were found in the proliferative diabetic retinopathy patients group.

scholarly journals ASSOCIATION OF MTHFR A1298C GENE VARIANT, DNA DAMAGE, AND TOTAL ANTIOXIDANT STATUS WITH THE RISK OF TYPE 2 DIABETES MELLITUS AND ITS COMPLICATIONS

2018 ◽  
Vol 11 (6) ◽  
pp. 344
Author(s):  
Nithya K ◽  
Isabel W ◽  
Angeline T ◽  
Priscilla A.s. ◽  
Asirvatham A.j.
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Dna Damage ◽  
Antioxidant Status ◽  
Total Antioxidant Status ◽  
Mthfr A1298c ◽  
Increased Risk ◽  
Significant Difference ◽  
Total Antioxidant

Objectives: We have examined the association of methylenetetrahydrofolate reductase (MTHFR) gene A1298C variant, DNA damage, and total antioxidant status (TAS) in patients with type 2 diabetes mellitus (T2DM) with and without complications and in healthy controls.Methods: A total of 300 subjects including 100 patients with complications, 100 patients without complications, and 100 controls were included. TAS was assessed by ferric reducing ability of plasma assay. DNA damage was analyzed in lymphocytes using the comet assay. Polymerase chain reaction-restriction fragment length polymorphism analysis was performed to study the MTHFR A1298C gene polymorphism among the study subjects.Results: The results revealed that the MTHFR 1298 AC+CC genotypes were associated with increased risk (2 fold) for diabetes and its complications. When the effect of DNA damage was analyzed, significant differences between individuals with mutant and normal genotype among the diabetic patients (with and without complications) was observed (p≤0.001). In contrary, no significant difference was found between TAS and 1298 genotypes (AA vs. AC+CC) in Type 2 diabetes patients (with and without complications), p=0.338. We also found a significant difference between the genotypes of the MTHFR A1298C and DNA damage, TAS in T2DM patients (with & without complications) when compared to controls, p<0.001.Conclusions: Our findings suggest that the MTHFR A1298C gene polymorphism is considered as a risk factor for the development of diabetes and its complications among south Indians. Therefore, increased DNA damage and decreased TAS along with the occurrence of a mutant genotype in an individual with diabetes may be at an increased risk for the development of chronic complications.

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