scholarly journals Qualitative and Quantitative Determination of Secondary Metabolites and Antioxidant Potential of Plumbago indica Root Extracts

2021 ◽  
pp. 319-325
Author(s):  
Kavita Mane ◽  
Rajendra Patil ◽  
Aditya Nath Pandey
Keyword(s):  
Ascorbic Acid ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Accurate Identification ◽  
Test Technique ◽  
Phytochemical Study ◽  
Plumbago Indica

Due to their well-known attribute of having minimal side effects as compared to medicines, natural items with medical potential are progressively gaining prominence in clinical research. The roots of Plumbago indica (P. indica, Plumbaginaceae) are commercially significant since they are the primary source of plumbagin and its derivatives. Plumbagin is well-known for its many pharmacological properties. P. indica roots yielded three naphthoquinones: plumbagin, 3, 30-biplumbagin, and elliptinone, which were employed as standard markers for quantitative HPLC analysis. The goal of this research was to screen phytochemicals, assess alkaloids, phenolic and flavonoid content, and measure the antioxidant potential of P. indica roots. The well-known test methodology was used to determine qualitative analysis of several phytochemical ingredients as well as quantitative analysis of total alkaloids, phenol, and flavonoids. The antioxidant activity of an ethanolic extract of P. indica roots was investigated in vitro using the 1,1-diphenyl, 2-picrylhydrazyl (DPPH) test technique. Alkaloids, glycosides, flavonoids, saponins, phenolics, proteins, and glucose were found in the ethanol and aqueous extracts, according to phytochemical study. P. indica roots ethanolic extract had 5.55, 0.930, and 3.940 mg of total phenolic, flavonoids, and alkaloids respectively. For comparative purposes, ascorbic acid was employed as a benchmark. In the investigated models, the extract showed dose-dependent free radical scavenging properties. For the DPPH technique, P. indica roots extract had an IC50 value of 23.02 µg/ml, which was equivalent to that of ascorbic acid (IC50=17.68 µg/ml). These researches contributed to the accurate identification of this plant material. The plant's broad variety of phytochemicals implies that it has medicinal potential, which might be investigated in the pharmaceutical sector as well as in traditional medicine.

scholarly journals Evaluation of Antioxidant Properties ofPhaulopsis fascisepalaC.B.Cl. (Acanthaceae)

2009 ◽  
Vol 6 (2) ◽  
pp. 227-231 ◽  
Author(s):  
S. A. Adesegun ◽  
A. Fajana ◽  
C. I. Orabueze ◽  
H. A. B. Coker
Keyword(s):  
Ascorbic Acid ◽  
Leaf Extract ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Chelating Ability

The antioxidant activities of crude extract ofPhaulopsis fascisepalaleaf were evaluated and compared with α-tocopherol and BHT as synthetic antioxidants and ascorbic acid as natural-based antioxidant.In vitro, we studied its antioxidative activities, radical-scavenging effects, Fe2+-chelating ability and reducing power. The total phenolic content was determined and expressed in gallic acid equivalent. The extract showed variable activities in all of thesein vitrotests. The antioxidant effect ofP. fascisepalawas strongly dose dependent, increased with increasing leaf extract dose and then leveled off with further increase in extract dose. Compared to other antioxidants used in the study, α-Tocopherol, ascorbic acid and BHT,P. fascisepalaleaf extract showed less scavenging effect on α,α,-diphenyl-β-picrylhydrazyl (DPPH) radical and less reducing power on Fe3+/ferricyanide complex but better Fe2+-chelating ability. These results revealed thein vitroantioxidant activity ofP.fascisepala.Further investigations are necessary to verify these activitiesin vivo.

Phytochemical Evaluation and In-vitro Antioxidant Potential of whole plant of Caralluma adscendens

2021 ◽  
pp. 2774-2778
Author(s):  
Divya Yada ◽  
T. Sivakkumar ◽  
M. Sudhakar
Keyword(s):  
Nitric Oxide ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Ethanolic Extract ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Antioxidant Power ◽  
Whole Plant

The current line of investigation was focused at perusing the presence of phytochemical constituents, investigation of total phenol and flavonoid content, the antioxidant potential of various extracts of Caralluma adscendens whole plant using various in-vitro assays. The dried plant powder was extracted with various solvents based on polarity (Pet ether, Chloroform, Ethyl acetate, Ethanol and Aqueous) by hot continuous extraction in Soxhlet's apparatus and Extracts were dried. Phytoconstituents present in each extract was examined by performing preliminary phytochemical screening. Total Phenolic Content (TPC), Total Flavonoid Content (TFC) and Antioxidant potential for crude extracts were studied by DPPH, nitric oxide scavenging and FRAP methods. The total phenolic content and flavonoid content of Ethanolic extract of plant was found to be 80.08±0.629mg and 70.88±1.170mg of GAE and Quercetin equivalents respectively. The Ethanolic extract exhibited potent antioxidant activity as determined by 2,2-diphenyl-1-picrylhydrazyl(DPPH), nitric oxide scavenging and ferric reducing antioxidant power assays(FRAP) than the other extracts. The IC50 values for the Ethanolic extract of Caralluma adscendens was found to be 214.765±0.224 µg/ml and 215.928±0.506µg/ml by DPPH and nitric oxide scavenging assays respectively.

scholarly journals ENHANCEMENT OF IN VITRO ANTIOXIDANT POTENTIAL OF TERMINALIA CHEBULA BY VARIOUS FRUIT EXTRACTS AND OPTIMIZATION OF CONCENTRATION BY RESPONSE SURFACE METHODOLOGY

2018 ◽  
Vol 11 (7) ◽  
pp. 228
Author(s):  
Geetika Sharma ◽  
Vipasha Sharma ◽  
Tulika Mishra
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Punica Granatum ◽  
Antioxidant Potential ◽  
Ananas Comosus ◽  
Terminalia Chebula ◽  
Fruit Extracts

Objective: The main objective of this study was to determine the enhancement of in vitro antioxidant potential of fruits of Terminalia chebula (TC) when used in combination with fruit extracts of Phyllanthus emblica, Ananas comosus, and Punica granatum. Hydroxyl (OH) radical scavenging and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assays were used to analyze the antioxidant potential.Method: Formulations of different combinatorial concentrations of fruits to prepare a mixture were achieved with central composite design through response surface methodology. Screening of 300 different combinations of various concentrations was done through hydroxyl radical scavenging assay followed by statistical analysis of data. Further validation of results was done by measuring the antioxidant potential of most bioactive extracts by DPPH method.Results: Screening of 300 samples of different combinations for antioxidant potential revealed the samples with highest percentage inhibition in aqueous (85.2%), ethanolic (92.9%), and aqueous-ethanolic (84.21%) extracts. Data were subjected to analysis of variance and generated a three-dimensional response surface plot for highest activity. Further subjecting these extracts to DPPH assay revealed a significant enhancement in the antioxidant potential of ethanolic extract of TC when used in mixture with other plants.Conclusion: Antioxidant activity of TC was enhanced when used in combination with other fruits extracts. These synergistic studies generating valuable interactions between various phytochemicals could lead to a momentous increase in other associated activities to fight against diseases such as cancer and cardiovascular disorders. Further research on isolation of bioactive compounds in the mixture and their potential to fight various types of cancer could lead to a significant augmentation in the activity of natural compounds.

scholarly journals Determination of Functional Properties of Sri Lankan Ambarella (Spondias dulcis Forst. syn. Spondias cytherea Sonn.) Fruit and Development of Vacuum Dried Ambarella Fruit Powder and Incorporated Soup Mix

2021 ◽  
pp. 113-122
Author(s):  
N. P. G. D. Navoda ◽  
M. D. W. Samaranayake ◽  
S. L. Liyanage ◽  
H. M. T. Herath ◽  
J. M. J. K. Jayasinghe
Keyword(s):  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Antioxidant Potential ◽  
Alpha Amylase ◽  
Total Phenolic ◽  
Sri Lankan ◽  
Antioxidant Power ◽  
Ethanolic Extracts ◽  
Fruit Powder

Objective: To develop vacuum dried (VD) Ambarella fruit powder and Ambarella fruit incorporated soup mix from large and miniature Ambarella and investigation of antioxidants and anti-diabetic properties. Methods: Ethanolic extracts of two Ambarella varieties were analyzed for antioxidant potential in terms of total phenolic content (TPC), total flavonoid content (TFC), ferrous reducing antioxidant power (FRAP), radical scavenging activities of DPPH and ABTS and α-amylase inhibitory activity. Results: Fresh dwarf Ambarella showed a significantly (p<0.05) high TPC (3.35±0.10 mgGAE/g) while fresh large Ambarella showed a significantly (p<0.05) high FRAP (0.71±0.13 mgTE/g) and DPPH (3.57±0.31 mg TE/g). In comparison of ethanolic extracts of VD Ambarella powders, a significantly (p<0.05) higher antioxidant potential in terms of FRAP (4.19±0.06 mgTE/g) exhibited in dwarf variety while significantly (p<0.05) higher ABTS (4.03±0.27 mgTE/g) and DPPH (3.00±0.49mgTE/g) exhibited in large Ambarella. Alpha amylase inhibition activities of fresh fruits of large and dwarf were 46.30±4.07% and 49.55±3.18% where as in VD powders were 27.59±5.03% and 15.58±5.86% respectively. An instant soup mixture was developed incorporating 20% of VD powder from large variety due its abundance. The antioxidant potential of ethanolic extract of the soup mixture in terms of TPC, TFC, ABTS, and FRAP were 0.55±0.00 mgGAE/g, 0.04±0.00 mgQE/g, 1.65±0.06 mgTE/g, and 0.04±0.15 mgTE/g. Alpha amylase inhibition activity of soup mixture was 39.49±0.29%. Conclusion: The both types of fresh fruits exhibited higher antioxidant potential except FRAP and higher anti-amylase inhibition than VD Ambarella powder.

scholarly journals Antioxidant capacity of Melissa Officinalis L. on Biological Systems

2018 ◽  
Vol 43 (3) ◽  
pp. 19
Author(s):  
Juliana Metzner Franco ◽  
Silvana Marina Piccoli Pugine ◽  
Antônio Márcio Scatoline ◽  
Mariza Pires De Melo
Keyword(s):  
Ascorbic Acid ◽  
Oxidative Damage ◽  
Antioxidant Capacity ◽  
Antioxidant Activities ◽  
Protective Action ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Melissa Officinalis ◽  
Frap Assay

The aim of the present study was to evaluate in vitro antioxidant capacity of Melissa extract (ME) (Melissa officinalis L.) and its protective effect on peroxyl radical-induced oxidative damage in erythrocytes. ME used in present study was obtained by rota-evaporation of the crude extract (ethanol:water/dried leaves). Total phenolic and flavonoids contend determination, 176.8 ± 13.2 mg GAE/g dw and  26.2 ± 3.2 mg QE/g dw, respectively).  Total equivalent antioxidant activities, TEAC in mg TE/g dw, were 61.4 ± 5.5 and 512.4 ± 77.2 for respective FRAP assay and DPPH• radical-scavenging. The ME acts as an antioxidant on NO and O2•-, when ME exerted a higher antioxidant action on NO scavenging to compared to the ascorbic acid (1.9 times), however, the antioxidant capacity of ME on O2•- was lower than ascorbic acid (5.6 times). The values of hemolysis inhibition from ME (IC50, 2.0 ± 0.5 mg/mL) were higher than ascorbic acid (IC50, 7.1 ± 1.8 mg/mL). Extract of Melissa was able to eliminate biological free radicals, suggesting a potential to prevent oxidative damage in vivo. In fact, the ME exerted protective action on cell membrane lysis in situ.

scholarly journals QUANTITATIVE STUDY OF TOTAL PHENOLIC AND ALKALOID CONTENT IN HYDROALCOHOLIC CONTENT IN RAUWOLFIA SERPENTINA

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Deepak Jain ◽  
Meenakshi Jain ◽  
Anurekha Jain
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Alcoholic Extract ◽  
Test Protocol ◽  
Rauwolfia Serpentina ◽  
Dpph Method

The objectives of this study are to screen the phytochemicals, estimate the content of flavonoid and alkaloids compounds and determine the antioxidant capacity of the Rauwolfia serpentina stem. Qualitative analysis of various phytochemical constituents and quantitative analysis of total phenol and alkaloids were determined by the well-known test protocol available in the literature. The hydro alcoholic extract of stem of Rauwolfia serpentina was studied for antioxidant activity on different in vitro models namely 1,1-diphenyl, 2-picryl hydrazyl (DPPH) method. Phytochemical analysis revealed the presence of phenols and flavonoids. The total flavonoids and alkaloids content of Rauwolfia serpentina stem of hydroalcoholic extract was 1.086 and 2.364mg/100mg respectively. Ascorbic acid used as standards was also evaluated for comparison. The extract showed dose dependent free radical scavenging property in the tested models. Rauwolfia serpentina stem extract showed IC50 value 68.10?g/ml for DPPH method, which was comparable to that of ascorbic acid (IC50=17.68?g/ml). The present study describes the phytochemical profile and antioxidant activity of Rauwolfia serpentina which will further used for medicinal applications.

scholarly journals Evaluation of Ethnopharmacological and Antioxidant Potential ofZanthoxylum armatumDC.

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Rabia Kanwal ◽  
Muhammad Arshad ◽  
Yamin Bibi ◽  
Saira Asif ◽  
Sunbal Khalil Chaudhari
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Antioxidant Potential ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Zanthoxylum Armatum

Zanthoxylum armatumDC. (syn.Z. alatumRoxb.) is an important medicinal plant commonly called Timur or Indian prickly ash. The ethnopharmacological study ofZ. armatumrevealed the use of different plant parts for curing various ailments including cholera, chest infection, fever, indigestion, stomach disorders, gas problems, piles, toothache, gum problems, dyspepsia, as carminative, antipyretic, aromatic, tonic, and stomachic. Keeping in view the medicinal potential of the plant, the antioxidant activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, reducing power, and phosphomolybdate assay using different concentrations (7.81 μg/mL–250 μg/mL). Ascorbic acid was taken as standard. The results indicated that the free radical scavenging activity ranged from 40.12% to 78.39%, and the reductive potential ranged from 0.265 nm to 1.411 nm while the total antioxidant activity ranged from 0.124 nm to 0.183 nm. The antioxidant potential evaluated by three assays increased in a concentration dependent manner and ascorbic acid showed better antioxidant activity than leaf extract. Results obtained through different tests confirmed redox protective activities ofZanthoxylum armatum. Further in vitro and in vivo research should be performed, so this plant can be further utilized in drug development.

scholarly journals The effect of elderly simulated gastrointestinal in vitro digestion (SGID) on the antioxidant properties of different cheese matrices

2020 ◽  
Vol 79 (OCE2) ◽  
Author(s):  
Aimee M. Plante ◽  
Aoife L. McCarthy ◽  
Fiona O'Halloran
Keyword(s):  
Oxidative Stress ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Physiological Changes ◽  
Negative Effects ◽  
Antioxidant Power ◽  
Age Related ◽  
Cheese Products

AbstractThe world's population is getting older. By 2050 it is projected that 22% of the global population will be over 60 years of age. Aging is characterized by a decline in physiological functions and an increase in malnutrition and associated disease. Oxidative stress is associated with several age-related conditions, including cardiovascular disease, obesity, neurodegenerative disease and cancer. The negative effects of reactive oxygen species (ROS) are neutralized by antioxidants, which can be endogenously synthesised or consumed through the diet. Cheese is a nutritious dairy food and previous research has highlighted the antioxidant potential of some cheese types. Physiological changes associated with age can impact digestion processes, thus the ability to efficiently release bioactive compounds, including antioxidants, may be impaired with age. Cheese products varying by milk source, texture and fat content were subjected to simulated gastrointestinal in vitro digestion (SGID) using a modified model to account for physiological changes associated with aging. The antioxidant potential of undigested and digested cheese (n = 11) was determined in vitro using DPPH radical scavenging, ferric reducing antioxidant power (FRAP) and total phenolic content (TPC) assays. Cellular antioxidant potential was also investigated using the reduced glutathione assay (GSH) in the human macrophage cell line (U937). Experiments were carried out in triplicate. Statistical analysis by T-test demonstrated that digestion significantly increased (P < 0.05) FRAP and TPC of all cheese products analysed. Of the eleven cheese products investigated, goats’ cheese displayed a significant increase (P < 0.05) in radical scavenging properties (89.8% inhibition). Low fat white cheddar had significantly higher (P < 0.05) GSH levels compared to full fat white cheddar (0.16 ± 0.01, 0.11 ± 0.01 μmol GSH/mg protein, respectively). Findings from this study suggest that despite physiological changes associated with age, cheese digestates retain, and in some cases, possess higher antioxidant potential than undigested samples. Cheese demonstrates potential as a functional food for the elderly by neutralizing the negative effects associated with age-related oxidative stress.

scholarly journals In-vitro Antioxidant and Anti-inflammatory Activities of Quisqualis indica Linn. Leaves Extract

2019 ◽  
pp. 1-13
Author(s):  
Pallavi Pal ◽  
Ajeet Singh
Keyword(s):  
Protein Denaturation ◽  
Radical Scavenging Activity ◽  
Diclofenac Sodium ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Standard Drug ◽  
Anti Inflammatory

Aim: In this study antioxidant and anti-inflammatory effect of ethanolic extract of Quisqualis indica leaves was evaluated. Study Design: In-vitro analysis of Quisqualis indica leaf extract. Place and Duration of Study: Molecular Biology laboratory, Department of Biotechnology, G.B Pant Engineering College, Pauri, between July 2015 and July 2016. Methods: Non-enzymatic and enzymatic assays such as DPPH (1, 1diphenyl-2-picryl hydrazyl), FRAP assay, superoxide dismutase SOD (EC 1.15.1.1), catalase (EC 1.11.1.6), for radical scavenging activity of ethanolic extracts of Quisqualis indica Linn. plant leaves had done. For estimation of anti-inflammatory action, two methods were employed: protein denaturation method and membrane stabilization method. Results: Ethanolic extract of leaves on higher concentration had better antioxidant potential when compared with reference standard ascorbic acid. They exhibited strong antioxidant radical scavenging activity values for ethanolic extract of leaves. Results of anti-inflammatory method suggested better potential values for ethanolic extract and compared with standard drug diclofenac sodium respectively. A significant relationship between antioxidant, anti-inflammatory capacity and total phenolic content was examined, indicating that phenolic compounds are the major contributors for the antioxidant and anti-inflammatory properties of this plant. Conclusion: Ethanolic extract of Q. indica exhibited strong anti-inflammatory and antioxidant activity and this can be used for designing novel drug inhibitors with better efficacy.

scholarly journals INVESTIGATION OF IN VITRO ANTIOXIDANT POTENTIAL OF METHANOLIC EXTRACT OF BARK OF PRUNUS CORNUTA AND ROOT OF RUMEX OBTUSIFOLIUS

2021 ◽  
Vol 21 (no 1) ◽  
Author(s):  
M.C purohit ◽  
Navneet Singh ◽  
Goutam Kumar ◽  
Rashmi Rawat ◽  
Mahender Singh
Keyword(s):  
Antioxidant Activity ◽  
Research Work ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Bark Extract ◽  
Total Phenolic ◽  
Root Extract ◽  
Rumex Obtusifolius ◽  
Methanolic Extracts

In the present research work, in vitro, the antioxidant activity of methanolic extracts of bark of Prunus cornuta and root of Rumex obtusifolius, respectively, was assessed by DPPH free radical scavenging method. Total phenolic content (TPC) of methanolic extracts was also determined by spectrophotometric method using Folin-Ciocalteu reagent and calculated in ?g/mL gallic acid equivalent (GAE). DPPH scavenging activity results show that the root extract of Rumex obtusifolius (IC50 = 17.15) and bark extract of Prunus cornuta (IC50 = 27.32) shows good antioxidant potential. TPC of root extract was found to be 178.56 ?g/g GAE, and bark extract was 137.23 ?g/g GAE. Antioxidant effect (%) determined by using phosphomolybdate assay and superoxide anion radical scavenging percentage further supports the potential antioxidant activity.

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