scholarly journals ESTIMATION THE ANTIBACTERIAL EFFECT OF CURCUMIN AND ROSEMARY AMONG PATIENTS WITH DENTAL CARIES IN VITRO

2019 ◽  
pp. 277-279
Author(s):  
EHAN ABDULHADI AL-SHARIFI ◽  
ASIA ABED AL-MAHMOOD ◽  
SUMAYAH AL-MAHMOOD
Keyword(s):  
Dental Caries ◽  
Aqueous Extract ◽  
Antibacterial Agents ◽  
Bacterial Culture ◽  
Antibacterial Effect ◽  
Anaerobic Bacteria ◽  
Oral Bacteria ◽  
Mannitol Salt Agar ◽  
Inhibition Zones

Objective: The aim is to estimate the effect of curcumin and rosemary as antibacterial agents among dental caries cases. Methods: Samples of saliva were randomly collected from 40 patients in Al-Furat General Hospital who attended the hospital from July to September 2018. Swabs were cultured on blood agar at 37°C for 24 h and then subcultured in mannitol salt agar and trypticase soy broth at 37°C for 24 h. Different concentrations of aqueous extract curcumin solution (0.1, 0.3, 0.5, and 1 mg/ml) and rosemary solution (1 g/ml) were prepared and added to the bacterial culture. Later, minimum inhibition zones of the bacterial cultures were determined. Results: The results showed that there were 25 cases of Streptococcus mutans, 10 cases of Staphylococcus aureus, 3 cases of anaerobic bacteria, and 2 cases of normal flora among 40 culturing swabs of bacteria. Aqueous extract of curcumin showed antibacterial effect with concentrations (0.1, 0.3, 0.5, and 1 mg/ml) against oral bacteria; nevertheless, these bacteria were resistant to the aqueous extract of rosemary with concentration 1 g/ml. Conclusion: It can be concluded that curcumin can be an effective antibacterial agent against dental caries disease and its effect increases positively in relation to its concentration. On the other hand, rosemary with 1 g/ml concentration did not show any effect on oral bacteria.

scholarly journals Nanocluster Structure of Pistacia atlantica subsp. Kurdica Turpentine and Its Antibacterial Effects

2020 ◽  
Vol 18 ◽  
Author(s):  
Ramyar Rahimi Darehbagh ◽  
Rojin Ramezani ◽  
Afra Hosseinpanahi ◽  
Ahmad Fotoohi ◽  
Samaneh Rouhi
Keyword(s):  
Antibacterial Agents ◽  
Direct Relationship ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
In Vivo Studies ◽  
Antibacterial Effects ◽  
Pistacia Atlantica ◽  
Inhibition Zones

Background: Medicinal herbs such as Pistacia atlantica (P. atlantica) subsp. Kurdica have antimicrobial effects. The present study is aimed to investigate the nanocluster structure of P. atlantica subsp. Kurdica turpentine and its composing elements and antibacterial effect. Methods: 100 μl ethanol was used to dissolve oily turpentine. 2, 2.2, 2.4, and 2.6 µg/μl of turpentine were used for investigating the antibacterial effects using disk and well diffusion methods. Elemental and nanocluster structure analyses were performed by Energy-Dispersive XRay Microanalysis (EDXMA) and Field Emission (FE)-scanning electron. Two-way analysis of variance (ANOVA) and Bonferoni test were used for data analysis (p ≤ 0.001). Results and Discussion: EDXMA elemental analysis of turpentine included: zinc (Zn), magnesium (Mg), fluorine (F), oxygen (O), silicium (Si), carbon (C), and argentum (Ag). A topography image of the turpentine showed a nanocluster surface with bright clusters in the background. The largest diameters of the growth inhibition zones (24.67 ± 0.58 mm in the disk diffusion and 23.67 ± 1.53 mm in the well diffusion) that were created by turpentine were observed against S. aureus ATCC 25923 at the concentration of 2.6 µg/μl. Diameter of the inhibition zone around bacterial growth had a direct relationship with turpentine concentration (p ≤ 0.001). Conclusion: The nanocluster structure of turpentine and its composed elements were detected in this research. Moreover, antibacterial effects of turpentine were proved. Herbal substances are widely used in medical applications. Different elements of P. atlantica subsp. Kurdica turpentine can be used as antibacterial agents, but more in-vitro and in-vivo studies should be performed in this field.

scholarly journals Antibacterial Effect of Juglans Regia Bark against Oral Pathologic Bacteria

2013 ◽  
Vol 2013 ◽  
pp. 1-5 ◽  
Author(s):  
Faramarz Zakavi ◽  
Leila Golpasand Hagh ◽  
Arash Daraeighadikolaei ◽  
Ahmad Farajzadeh Sheikh ◽  
Arsham Daraeighadikolaei ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Aqueous Extract ◽  
Antibacterial Effect ◽  
Juglans Regia ◽  
Ethanolic Extract ◽  
Negative Control ◽  
Oral Bacteria ◽  
Disk Diffusion ◽  
Resistant Bacteria ◽  
Aqueous Extracts

Background. In this study antimicrobial effect of ethanolic and aqueous extracts of Juglans regia bark in Iran was evaluated on four different oral bacteria,Streptococcus mutans,Streptococcus salivarius,Streptococcus sanguis, andStaphylococcus aureus.Methods. Aqueous and ethanol extracts of Juglans regia bark were prepared by using disk diffusion technique and Minimal Inhibitory Concentration (MIC) methods. Tetracycline 30 μg and Erythromycin 15 μg were used as positive control and water as negative control in disk diffusion and MIC methods. Data were analyzed by ANOVA test.Results. The results showed thatS. sanguisandS. mutanswere the most sensitive and the most resistant bacteria against ethanolic and aqueous extracts, respectively. Ethanolic extract had significant antibacterial effect against all tested bacteria. Aqueous extract did not show antibacterial effect onS. mutans, in contrast to ethanolic extract. Aqueous extract had significantly antibacterial effect againstStaphylococcus aureus,S. salivarius, andS. sanguiscompared to control (P<0.0001), but it did not show effect onS. mutanswhen compared with Erythromycin. According to the obtained MIC values, ethanol extract of Juglans regia bark had the lowest rate.Conclusion. The results may provide the basis for using natural antimicrobial substance for oral hygiene prophylaxis purposes.

scholarly journals Characterization of Recombinant, Ureolytic Streptococcus mutans Demonstrates an Inverse Relationship between Dental Plaque Ureolytic Capacity and Cariogenicity

2000 ◽  
Vol 68 (5) ◽  
pp. 2621-2629 ◽  
Author(s):  
K. Anne Clancy ◽  
Sylvia Pearson ◽  
William H. Bowen ◽  
Robert A. Burne
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Urease Activity ◽  
Dental Health ◽  
Oral Bacteria ◽  
Streptococcus Salivarius ◽  
Specific Pathogen ◽  
The Relationship

ABSTRACT Dental caries results from prolonged plaque acidification that leads to the establishment of a cariogenic microflora and demineralization of the tooth. Urease enzymes of oral bacteria hydrolyze urea to ammonia, which can neutralize plaque acids. To begin to examine the relationship between plaque ureolytic activity and the incidence of dental caries, recombinant, ureolytic strains ofStreptococcus mutans were constructed. Specifically, theureABCEFGD operon from Streptococcus salivarius57.I was integrated into the S. mutans chromosome in such a way that the operon was transcribed from a weak, cognate promoter inS. mutans ACUS4 or a stronger promoter in S. mutans ACUS6. Both strains expressed NiCl2-dependent urease activity, but the maximal urease levels in ACUS6 were threefold higher than those in ACUS4. In vitro pH drop experiments demonstrated that the ability of the recombinant S. mutans strains to moderate a decrease in pH during the simultaneous metabolism of glucose and urea increased proportionately with the level of urease activity expressed. Specific-pathogen-free rats that were infected with ACUS6 and fed a cariogenic diet with drinking water containing 25 mM urea and 50 μM NiCl2 had relatively high levels of oral urease activity, as well as dramatic decreases in the prevalence of smooth-surface caries and the severity of sulcal caries, relative to controls. Urease activity appears to influence plaque biochemistry and metabolism in a manner that reduces cariogenicity, suggesting that recombinant, ureolytic bacteria may be useful to promote dental health.

In Vitro Inhibition of Bacterial Growth Using Different Dental Adhesive Systems

2007 ◽  
Vol 32 (4) ◽  
pp. 388-393 ◽  
Author(s):  
R. Walter ◽  
W. R. Duarte ◽  
P. N. R. Pereira ◽  
H. O. Heymann ◽  
E. J. Swift ◽  
...  
Keyword(s):  
Bacterial Growth ◽  
Clinical Relevance ◽  
Antibacterial Effect ◽  
Oral Bacteria ◽  
Adhesive Systems ◽  
Dental Adhesive ◽  
In Vitro Inhibition

Clinical Relevance All materials tested, especially iBond, have a potential long-term antibacterial effect against the oral bacteria tested.

scholarly journals Antibacterial Activity of Buah Merah (Pandanus conoideus Lam.) Against Bacterial Oral Pathogen of Streptococcus sanguinis ATCC10556, Streptococcus mutans ATCC 25175, and Enterococcus faecalis ATCC 29212: An in Vitro Study

2020 ◽  
Vol 14 (1) ◽  
pp. 113-119 ◽  
Author(s):  
Lisda Damayanti ◽  
Ida Ayu Evaangelina ◽  
Avi Laviana ◽  
Yetty Herdiyati ◽  
Dikdik Kurnia
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Synergistic Effects ◽  
In Vitro Study ◽  
Inhibition Zone ◽  
Oral Bacteria ◽  
Inhibition Zones

Background: Caries and periodontitis are dental diseases caused by bacteria of S. sanguinis, S. mutans, and E. faecalis with three main etiological factors of the host, substrate, and time. Objective: This study proposed to investigate the antibacterial effects of Buah Merah (Pandanus conoideus Lam.) against oral bacteria of E.faecalis, S. mutans, and S. sanguinis. Materials and Methods: The Buah Merah was extracted with different solvents to yield n-hexane, ethyl acetate, methanol, and H2O extracts. The concentrations of single and mixture extracts were adjusted for antibacterial assay against bacteria of E. faecalis, S. mutans, and S. sanguinis strains through agar well diffusion assay with chlorhexidine, fosfomycin, and quercetin used as positive controls. Results: The ethyl acetate extract showed highest antibacterial activity against three oral bacterial of E. faecalis, S. mutans, and S. sanguinis with inhibition zones values of 9.3, 12.3, and 17.9 mm at 40%, respectively, together with their MIC and MBC values of 1250 & 2500, 0.312 & 0.625, and 0.312 & 0.625 ppm, respectively. For the formulation of extracts, combinations samples test gave various effects to different bacteria, with the best activity showed by methanol-ethyl acetate (M-Ea) extracts against S. mutans with an inhibition zone of 16.25 mm at 40 ppm. The strong and synergistic effect of methanol extract against S. mutans was supported by inhibition zones of the formulation of methanol extract-fosfomycin which showed an inhibition zone of 25.9 mm at 10 ppm. Conclusion: The extracts of Buah Merah demonstrated antibacterial activity against oral bacteria of E. faecalis, S. mutans, and S. sanguinis and gave important information for further in vivo clinical studies to determine the exact dosages and its effectiveness in practical application. These results prove the antimicrobial effects of Buah Merah extracts as alternative natural drugs with synergistic effects of active constituents.

scholarly journals A simplified in vitro model for investigation of the antimicrobial efficacy of various antiseptic agents to prevent peri-implantitis

2020 ◽  
pp. 1-6
Author(s):  
Annamária Venkei ◽  
Gabriella EÖrdegh ◽  
Kinga Turzó ◽  
Edit Urbán ◽  
Krisztina Ungvári
Keyword(s):  
Antibacterial Effect ◽  
Treatment Time ◽  
Colorimetric Assay ◽  
Oral Bacteria ◽  
Polished Surface ◽  
Ultrapure Water ◽  
Implant Surface ◽  
Titanium Surfaces ◽  
Vitro Model

Abstract The biofilm formation by oral bacteria on the implant surface is one of the most remarkable factors of peri-implant infections, which may eventually lead to bone resorption and loss of the dental implant. Therefore, the elimination of biofilm is an essential step for the successful therapy of implant-related infections. In this work we created a basic in vitro model to evaluate the antibacterial effect of three widely used antiseptics. Commercially pure (CP4) titanium sample discs with sand blasted, acid etched, and polished surface were used. The discs were incubated with mono-cultures of Streptococcus mitis and Streptococcus salivarius. The adhered bacterial biofilms were treated with different antiseptics: chlorhexidine-digluconate (CHX), povidone-iodine (PI), and chlorine dioxide (CD) for 5 min and the control discs with ultrapure water. The antibacterial effect of the antiseptics was tested by colorimetric assay. According to the results, the PI and the CD were statistically the most effective in the elimination of the two test bacteria on both titanium surfaces after 5 min treatment time. The CD showed significant effect only against S. salivarius. Based on our results we conclude that PI and CD may be promising antibacterial agents to disinfecting the peri-implant site in the dental practice.

scholarly journals Dental Pulp Defence and Repair Mechanisms in Dental Caries

2015 ◽  
Vol 2015 ◽  
pp. 1-16 ◽  
Author(s):  
Jean-Christophe Farges ◽  
Brigitte Alliot-Licht ◽  
Emmanuelle Renard ◽  
Maxime Ducret ◽  
Alexis Gaudin ◽  
...  
Keyword(s):  
Dental Caries ◽  
Dental Pulp ◽  
Molecular Mechanisms ◽  
Inflammatory Responses ◽  
Oral Bacteria ◽  
Barrier Formation ◽  
Reparative Dentin ◽  
Pulp Inflammation

Dental caries is a chronic infectious disease resulting from the penetration of oral bacteria into the enamel and dentin. Microorganisms subsequently trigger inflammatory responses in the dental pulp. These events can lead to pulp healing if the infection is not too severe following the removal of diseased enamel and dentin tissues and clinical restoration of the tooth. However, chronic inflammation often persists in the pulp despite treatment, inducing permanent loss of normal tissue and reducing innate repair capacities. For complete tooth healing the formation of a reactionary/reparative dentin barrier to distance and protect the pulp from infectious agents and restorative materials is required. Clinical andin vitroexperimental data clearly indicate that dentin barrier formation only occurs when pulp inflammation and infection are minimised, thus enabling reestablishment of tissue homeostasis and health. Therefore, promoting the resolution of pulp inflammation may provide a valuable therapeutic opportunity to ensure the sustainability of dental treatments. This paper focusses on key cellular and molecular mechanisms involved in pulp responses to bacteria and in the pulpal transition between caries-induced inflammation and dentinogenic-based repair. We report, using selected examples, different strategies potentially used by odontoblasts and specialized immune cells to combat dentin-invading bacteriain vivo.

scholarly journals Isolation and Characterization of anti-Streptococcus Mutans Phage as a Possible Treatment Agent for Caries

2021 ◽  
Author(s):  
Hadar Ben Zaken ◽  
Reut Kraitman ◽  
Shunit Coppenhagen-Glazer ◽  
Leron Khalifa ◽  
Sivan Alkalay-Oren ◽  
...  
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Pathogenic Bacteria ◽  
Antibacterial Effect ◽  
Antibacterial Properties ◽  
Human Saliva ◽  
Whole Genome Sequence ◽  
Isolation And Characterization

Streptococcus mutans is a key bacterium in dental caries- one of the most prevalent chronic infectious diseases. Conventional treatment both fails to specifically target the pathogenic bacteria and at-tempts to eradicate commensal bacteria as well. Thus, caries remains one of most common and challenging diseases. The use of bacterial viruses as anti-bacterial agents, is gaining interest worldwide. Hardly any phages were described against S. mutans. The objective of this study was to isolate anti-S. mutans phages and to characterize their antimi-crobial properties. Human saliva samples were filtered and screened for potential phages. Standard double-layered agar method was used for isolation. Whole genome sequence analysis and morphology visualization by TEM, were used for anti-S. mutans phage identification. Antibacterial properties were evaluated using clinical strains and ATCC strains of S. mutans in various states. Antibacterial effect was also tested on human cariogenic dentin. One phage against S. mutans was isolated and termed SMHBZ8. This phage showed effective lytic activity in vitro against both planktonic and biofilm S. mutans cultures. Moreover, the phage showed antibacterial effect when used on cariogenic dentin. The isolation and characterization of SMHBZ8 may be the first step in developing a potential phage therapy for dental caries.

Antibacterial effect of bioactive glasses on clinically important anaerobic bacteria in vitro

2007 ◽  
Vol 19 (2) ◽  
pp. 547-551 ◽  
Author(s):  
Outi Leppäranta ◽  
Minna Vaahtio ◽  
Timo Peltola ◽  
Di Zhang ◽  
Leena Hupa ◽  
...  
Keyword(s):  
Antibacterial Effect ◽  
Anaerobic Bacteria ◽  
Bioactive Glasses

scholarly journals Evaluation of Antibacterial Effectiveness of Desensitizers against Oral Bacteria

2008 ◽  
Vol 02 (01) ◽  
pp. 43-47 ◽  
Author(s):  
Ismet Duran ◽  
Abdulkadir Sengun ◽  
Hasan Huseyin Hadimli ◽  
Mustafa Ulker
Keyword(s):  
Antibacterial Effect ◽  
Clinical Results ◽  
Antibacterial Activities ◽  
Bactericidal Effect ◽  
Oral Bacteria ◽  
Significance Level ◽  
Inhibition Zones ◽  
Acidic Components ◽  
One Way Anova ◽  
Very High

ABSTRACTObjectives: Desensitizers contribute to better clinical results by reducing the rate of cervical dentin sensitivity. However, information on their antibacterial effect is limited. This study examined the antibacterial activities of a triclosan containing (Seal & Protect), a benzalconium containing desensitizer (Micro Prime), a fluoride containing prophilaxy paste (Sultan Desensitizer), two fluoride containing varnishes (Cavity Shealth and Ultra EZ), and a dentin bonding primer (All Bond).Methods: The test materials were inserted in the wells of Muller Hinton agar plates inoculated with Streptococcus mutans, Streptococcus salivarious, Staphylococcus aureus, Streptococcus faecalis and Pseudomonas aeruginosa. The diameters of the inhibition zones produced around the materials were measured after 24 h of incubation. The results were analyzed by the Kruskal Wallis one way ANOVA and the Mann-Whitney tests at a significance level of P<.05.Results: Micro Prime Desensitizer containing benzalkonium chloride had the highest antibacterial effectiveness compared to other desensitizers used in this study. In addition, triclosan containing Seal & Protect and acidic components containing All Bond showed very high antibacterial efficacy. On the other hand, fluoride within both varnishes had little antibacterial effectiveness. However a fluoride component in a paste (Sultan Desensitizer) showed very high bactericidal effect.Conclusions: All desensitizers except fluoride varnishes showed various degrees of antibacterial effect against the bacteria tested in this study. If antibacterial effect is also required from the desensitizers’ clinicians should avoid use of varnishes. (Eur J Dent 2008;2:43-47)

Sign in / Sign up

Export Citation Format

Share Document