scholarly journals A simplified in vitro model for investigation of the antimicrobial efficacy of various antiseptic agents to prevent peri-implantitis

2020 ◽  
pp. 1-6
Author(s):  
Annamária Venkei ◽  
Gabriella EÖrdegh ◽  
Kinga Turzó ◽  
Edit Urbán ◽  
Krisztina Ungvári
Keyword(s):  
Antibacterial Effect ◽  
Treatment Time ◽  
Colorimetric Assay ◽  
Oral Bacteria ◽  
Polished Surface ◽  
Ultrapure Water ◽  
Implant Surface ◽  
Titanium Surfaces ◽  
Vitro Model

Abstract The biofilm formation by oral bacteria on the implant surface is one of the most remarkable factors of peri-implant infections, which may eventually lead to bone resorption and loss of the dental implant. Therefore, the elimination of biofilm is an essential step for the successful therapy of implant-related infections. In this work we created a basic in vitro model to evaluate the antibacterial effect of three widely used antiseptics. Commercially pure (CP4) titanium sample discs with sand blasted, acid etched, and polished surface were used. The discs were incubated with mono-cultures of Streptococcus mitis and Streptococcus salivarius. The adhered bacterial biofilms were treated with different antiseptics: chlorhexidine-digluconate (CHX), povidone-iodine (PI), and chlorine dioxide (CD) for 5 min and the control discs with ultrapure water. The antibacterial effect of the antiseptics was tested by colorimetric assay. According to the results, the PI and the CD were statistically the most effective in the elimination of the two test bacteria on both titanium surfaces after 5 min treatment time. The CD showed significant effect only against S. salivarius. Based on our results we conclude that PI and CD may be promising antibacterial agents to disinfecting the peri-implant site in the dental practice.

scholarly journals An In Vitro Model for Candida albicans–Streptococcus gordonii Biofilms on Titanium Surfaces

2018 ◽  
Vol 4 (2) ◽  
pp. 66 ◽  
Author(s):  
Daniel Montelongo-Jauregui ◽  
Anand Srinivasan ◽  
Anand Ramasubramanian ◽  
Jose Lopez-Ribot
Keyword(s):  
Candida Albicans ◽  
In Vitro Model ◽  
Streptococcus Gordonii ◽  
Titanium Surfaces ◽  
Vitro Model

scholarly journals ESTIMATION THE ANTIBACTERIAL EFFECT OF CURCUMIN AND ROSEMARY AMONG PATIENTS WITH DENTAL CARIES IN VITRO

2019 ◽  
pp. 277-279
Author(s):  
EHAN ABDULHADI AL-SHARIFI ◽  
ASIA ABED AL-MAHMOOD ◽  
SUMAYAH AL-MAHMOOD
Keyword(s):  
Dental Caries ◽  
Aqueous Extract ◽  
Antibacterial Agents ◽  
Bacterial Culture ◽  
Antibacterial Effect ◽  
Anaerobic Bacteria ◽  
Oral Bacteria ◽  
Mannitol Salt Agar ◽  
Inhibition Zones

Objective: The aim is to estimate the effect of curcumin and rosemary as antibacterial agents among dental caries cases. Methods: Samples of saliva were randomly collected from 40 patients in Al-Furat General Hospital who attended the hospital from July to September 2018. Swabs were cultured on blood agar at 37°C for 24 h and then subcultured in mannitol salt agar and trypticase soy broth at 37°C for 24 h. Different concentrations of aqueous extract curcumin solution (0.1, 0.3, 0.5, and 1 mg/ml) and rosemary solution (1 g/ml) were prepared and added to the bacterial culture. Later, minimum inhibition zones of the bacterial cultures were determined. Results: The results showed that there were 25 cases of Streptococcus mutans, 10 cases of Staphylococcus aureus, 3 cases of anaerobic bacteria, and 2 cases of normal flora among 40 culturing swabs of bacteria. Aqueous extract of curcumin showed antibacterial effect with concentrations (0.1, 0.3, 0.5, and 1 mg/ml) against oral bacteria; nevertheless, these bacteria were resistant to the aqueous extract of rosemary with concentration 1 g/ml. Conclusion: It can be concluded that curcumin can be an effective antibacterial agent against dental caries disease and its effect increases positively in relation to its concentration. On the other hand, rosemary with 1 g/ml concentration did not show any effect on oral bacteria.

scholarly journals Preparation of Bioactive Titanium Surfaces via Fluoride and Fibronectin Retention

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Carlos Nelson Elias ◽  
Patricia Abdo Gravina ◽  
Costa e Silva Filho ◽  
Pedro Augusto de Paula Nascente
Keyword(s):  
Dental Implants ◽  
Dental Implant ◽  
Osteoblastic Cells ◽  
Acid Etching ◽  
Implant Surface ◽  
Force Microscopy ◽  
Dental Implant Surface ◽  
Before And After ◽  
Titanium Surfaces

Statement of Problem. The chemical or topographic modification of the dental implant surface can affect bone healing, promote accelerated osteogenesis, and increase bone-implant contact and bonding strength.Objective. In this work, the effects of dental implant surface treatment and fibronectin adsorption on the adhesion of osteoblasts were analyzed.Materials and Methods. Two titanium dental implants (Porous-acid etching and PorousNano-acid etching followed by fluoride ion modification) were characterized by high-resolution scanning electron microscopy, atomic force microscopy, and X-ray diffraction before and after the incorporation of human plasma fibronectin (FN). The objective was to investigate the biofunctionalization of these surfaces and examine their effects on the interaction with osteoblastic cells.Results. The evaluation techniques used showed that the Porous and PorousNano implants have similar microstructural characteristics. Spectrophotometry demonstrated similar levels of fibronectin adsorption on both surfaces (80%). The association indexes of osteoblastic cells in FN-treated samples were significantly higher than those in samples without FN. The radioactivity values associated with the same samples, expressed as counts per minute (cpm), suggested that FN incorporation is an important determinant of thein vitrocytocompatibility of the surfaces.Conclusion. The preparation of bioactive titanium surfaces via fluoride and FN retention proved to be a useful treatment to optimize and to accelerate the osseointegration process for dental implants.

In Vitro Inhibition of Bacterial Growth Using Different Dental Adhesive Systems

2007 ◽  
Vol 32 (4) ◽  
pp. 388-393 ◽  
Author(s):  
R. Walter ◽  
W. R. Duarte ◽  
P. N. R. Pereira ◽  
H. O. Heymann ◽  
E. J. Swift ◽  
...  
Keyword(s):  
Bacterial Growth ◽  
Clinical Relevance ◽  
Antibacterial Effect ◽  
Oral Bacteria ◽  
Adhesive Systems ◽  
Dental Adhesive ◽  
In Vitro Inhibition

Clinical Relevance All materials tested, especially iBond, have a potential long-term antibacterial effect against the oral bacteria tested.

Graphene Oxide Enables the Reosteogenesis of Previously Contaminated Titanium In Vitro

2020 ◽  
Vol 99 (8) ◽  
pp. 922-929
Author(s):  
W. Qin ◽  
C. Wang ◽  
C. Jiang ◽  
J. Sun ◽  
C. Yu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Graphene Oxide ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Potential ◽  
Implant Surface ◽  
Significant Difference ◽  
Titanium Surfaces ◽  
Group B

The main goal of peri-implantitis treatment is to control infection and arrest bone loss, which requires the removal of polymicrobial biofilms on the implant surface and the reduction of tissue invasion. Additionally, prognosis can be improved if reosseointegration occurs on previously contaminated implants. To evaluate whether graphene oxide (GO) can remove polymicrobial biofilms, biofilms were established on titanium surfaces in vitro and treated with different methods: group B, removed only with brushing; group G, treated with different GO concentrations (64, 128, 256, and 512 μg/mL); group GB, combined treatments of groups B and G; and group C, untreated. Subsequently, to evaluate reosteogenesis on previously contaminated titanium, 4 groups were used: groups C, B, GB-256, and GB-512 (treated with 256 and 512 μg/mL of GO, respectively). Intact clean titanium (IC) was used as a control. Additionally, cell behavior on IC treated with GB-256 (IGB-256) and GB-512 (IGB-512) was compared with that of the GB-256 and GB-512 groups, respectively. The results showed that at high concentrations (≥256 μg/mL), GO eliminated residual bacteria and inhibited biofilm reformation after brushing, whereas neither GO nor brushing alone could achieve this. Bone marrow–derived mesenchymal stem cell viability in groups GB-256 and IC was higher than that in groups GB-512, C, and B ( P < 0.05). No significant difference was found between group GB-256 and group IC ( P > 0.05). Osteogenic differentiation of bone marrow–derived mesenchymal stem cells in group GB-256 was higher than that in groups IC, GB-512, C, and B. No difference was found between groups IGB-256 and IGB-512 and groups GB-256 and GB-512, respectively ( P > 0.05). In conclusion, 256 μg/mL of GO combined with brushing significantly removed polymicrobial biofilms that remained on the previously contaminated titanium surfaces. The bone marrow–derived mesenchymal stem cell osteogenic potential was regained or even enhanced on the titanium surfaces treated this way in vitro, which might provide a new idea for treating peri-implantitis.

scholarly journals Oral Streptococci Biofilm Formation on Different Implant Surface Topographies

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Pedro Paulo Cardoso Pita ◽  
José Augusto Rodrigues ◽  
Claudia Ota-Tsuzuki ◽  
Tatiane Ferreira Miato ◽  
Elton G. Zenobio ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Surface Characterization ◽  
Bacterial Species ◽  
Oral Streptococci ◽  
Streptococcus Sobrinus ◽  
Implant Surface ◽  
Surface Topographies ◽  
Unstimulated Saliva ◽  
Titanium Surfaces

The establishment of the subgingival microbiota is dependent on successive colonization of the implant surface by bacterial species. Different implant surface topographies could influence the bacterial adsorption and therefore jeopardize the implant survival. This study evaluated the biofilm formation capacity of five oral streptococci species on two titanium surface topographies.In vitrobiofilm formation was induced on 30 titanium discs divided in two groups: sandblasted acid-etched (SAE-n=15) and as-machined (M-n=15) surface. The specimens were immersed in sterilized whole human unstimulated saliva and then in fresh bacterial culture with five oral streptococci species:Streptococcus sanguinis,Streptococcus salivarius,Streptococcus mutans,Streptococcus sobrinus, andStreptococcus cricetus. The specimens were fixed and stained and the adsorbed dye was measured. Surface characterization was performed by atomic force and scanning electron microscopy. Surface and microbiologic data were analyzed by Student’st-test and two-way ANOVA, respectively (P<0.05).S. cricetus,S. mutans,andS. sobrinusexhibited higher biofilm formation and no differences were observed between surfaces analyzed within each species (P>0.05).S. sanguinisexhibited similar behavior to form biofilm on both implant surface topographies, whileS. salivariusshowed the lowest ability to form biofilm. It was concluded that biofilm formation on titanium surfaces depends on surface topography and species involved.

Biofilm Formation by Pseudomonas Species Onto Graphene Oxide–TiO2 Nanocomposite-Coated Catheters: In vitro Analysis

2017 ◽  
Vol 17 (01n02) ◽  
pp. 1760014
Author(s):  
Ananya Deb ◽  
R. Vimala
Keyword(s):  
Graphene Oxide ◽  
Titanium Oxide ◽  
Biofilm Formation ◽  
Colorimetric Assay ◽  
Foley Catheter ◽  
Tetrazolium Salt ◽  
Biofilm Growth ◽  
Vitro Model ◽  
Vitro Analysis

The present study focuses on the development of an in vitro model system for biofilm growth by Pseudomonas aerouginosa onto small discs of foley catheter. Catheter disc used for the study was coated with graphene oxide–titanium oxide composite (GO–TiO[Formula: see text] and titanium oxide (TiO[Formula: see text] and characterized through XRD, UV–visible spectroscopy. Morphological analysis was done by scanning electron microscopy (SEM). The biofilm formed on the catheter surface was quantified by crystal violet (CV) staining method and a colorimetric assay (MTT assay) which involves the reduction of tetrazolium salt. The catheter coated with GO–TiO2 showed reduced biofilm growth in comparison to the TiO2-coated and uncoated catheter, thus indicating that it could be successfully used in coating biomedical devices to prevent biofilm formation which is a major cause of nosocomial infection.

scholarly journals Shape-Depended Biological Properties of Ag3PO4 Microparticles: Evaluation of Antimicrobial Properties and Cytotoxicity in In Vitro Model—Safety Assessment of Potential Clinical Usage

2019 ◽  
Vol 2019 ◽  
pp. 1-19 ◽  
Author(s):  
Karol P. Steckiewicz ◽  
Julia Zwara ◽  
Maciej Jaskiewicz ◽  
Szymon Kowalski ◽  
Wojciech Kamysz ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Biomedical Application ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Biological Properties ◽  
Economic Problem ◽  
Cell Cycle Distribution ◽  
Implant Surface ◽  
Vitro Model

Implant-related infections are an emerging clinical and economic problem. Therefore, we decided to assess potential clinical usefulness and safety of silver orthophosphate microparticles (SOMPs) regarding their shape. We synthesized and then assessed antimicrobial properties and potential cytotoxicity of six shapes of SOMPs (tetrapod, cubes, spheres, tetrahedrons, branched, and rhombic dodecahedron). We found that SOMPs had a high antimicrobial effect; they were more efficient against fungi than bacteria. SOMPs exerted an antimicrobial effect in concentrations not toxic to mammalian cells: human fetal osteoblast (hFOB1.19), osteosarcoma (Saos-2), mouse preosteoblasts (MC3T3-E1), skin fibroblast (HDF), and mouse myoblast (C2C12). At higher concentration SOMPs, induced shape- and concentration-dependent cytotoxicity (according to MTT and BrdU assays). Tetrapod SOMPs had the smallest effect, whereas cubical SOMPs, the highest on cell viability. hFOB1.19 were the most resistant cells and C2C12, the most susceptible ones. We have proven that the induction of oxidative stress and inflammation is involved in the cytotoxic mechanism of SOMPs. After treatment with microparticles, we observed changes in levels of reactive oxygen species, first-line defense antioxidants-superoxide dismutase (SOD1, SOD3), and glutathione peroxidase (GPX4), metalloproteinase (MMP1, MMP3), and NF-κB protein. Neither cell cycle distribution nor ultrastructure was altered as determined by flow cytometry and transmission electron microscopy, respectively. In conclusion, silver orthophosphate may be a safe and effective antimicrobial agent on the implant surface. Spherical-shaped SOMPs are the most promising for biomedical application.

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