scholarly journals IN VIVO AND IN VITRO INVESTIGATIONS OF PHARMACOLOGICAL POTENTIALS OF CASSIA OBTUSIFOLIA PLANT

2020 ◽  
pp. 87-94
Author(s):  
KEWATKAR SM ◽  
PAITHANKAR VV ◽  
BHUJBAL SS ◽  
JAIN SP ◽  
NAGORE DH
Keyword(s):  
Pharmacological Study ◽  
Immersion Method ◽  
Cassia Obtusifolia ◽  
The Family ◽  
Tail Immersion ◽  
Anti Inflammatory ◽  
Pharmacological Potential ◽  
One Way Anova

Objectives: Cassia obtusifolia L. belonging to the Family Caesalpiniaceae, proposed to have abundant pharmacological potential and widely consumed as laxative, diuretic, and stomachic. The aim of the present research was to study the anti-inflammatory, analgesic, antipyretic, and antioxidant potentials of C. obtusifolia plant. Methods: Various acute and chronic animal models such as Carrageenan-induced paw edema, tail immersion method, acetic acid-induced writhing, and in vitro methods were used to study the profound pharmacological and antioxidant potentials. Results: The results for pharmacological study were statistically analyzed by one-way ANOVA followed by Dunnett’s multiple comparisons using INTA software. Conclusion: The present study reveals that C. obtusifolia possesses comparable anti-inflammatory, analgesic, antipyretic, and antioxidant potential.

scholarly journals In vitro and in vivo Evaluation of Pharmacological Potential of Lasia spinosa Linn.

2021 ◽  
Vol 20 (1) ◽  
pp. 111-119
Author(s):  
Piyali Chowdhury ◽  
Mohammed Ibrahim ◽  
Sarrin Shahadat ◽  
Md Ruhul Kuddus ◽  
Mohammad A Rashid
Keyword(s):  
Body Weight ◽  
Analgesic Activity ◽  
Chloroform Fraction ◽  
Immersion Method ◽  
Rbc Membrane ◽  
Activity Test ◽  
Tail Immersion ◽  
Anti Inflammatory

Lasia spinosa Linn. (Family: Araceae) is an important medicinal plant, which is traditionally used for treatment of different human ailments. The present study was undertaken to evaluate the in vitro thrombolytic, antiinflammatory and in vivo analgesic and hypoglycemic potentials of n-hexane, chloroform and aqueous soluble fractions of methanol extract of L. spinosa whole plant. Additionally, phytochemical screening was carried out by qualitative tests, which confirmed the presence of alkaloids, glycosides, steroids, tannins, saponin in this plant. During in vitro thrombolytic assay, the aqueous fraction at a dose of 500 μg/100 μl showed the maximum 33.15% lysis of the blood clot, as compared to the standard streptokinase (80.10%). The in vitro anti-inflammatory test was performed by inhibition of egg albumin denaturation assay and RBC membrane stabilization method. The chloroform fraction exhibited maximum anti-inflammatory potential by inhibiting 51.53% denaturation of albumin and by inhibiting 54.8% hemolysis of RBC membrane against hypotonic solution. Analgesic activity was evaluated by tail immersion method for central mechanism and by formalin-induced lick test for peripheral mechanism in mice. In tail immersion method, all the solvent fractions of L. spinosa at a dose of 500 mg/kg body weight exhibited a significant (p<0.05) elongation in pain reaction time. In peripheral analgesic activity test, the chloroform fraction at a dose of 500 mg/kg body weight inhibited a maximum of 35.44% licking response induced by formalin, as compared to the standard aspirin (53.22%). In the hypoglycemic activity test, all the fractions showed a moderate effect in reducing the blood glucose level in mice treated with 10% glucose. In conclusion, the plant L. spinosa can be considered as a promising source of bioactive compounds for the development of new phytomedicine. Dhaka Univ. J. Pharm. Sci. 20(1): 111-119, 2021 (June)

scholarly journals In vitro Anti-inflammatory Properties in Various Extracts (Ethanol, Chloroform and Aqueous) of Kaempferia galanga Linn Rhizome

2021 ◽  
pp. 476-481
Author(s):  
Hooriyah Laiq Ahmed Khan ◽  
G. Sridevi ◽  
J. Selvaraj ◽  
S. Preetha
Keyword(s):  
Statistical Analysis ◽  
Protein Denaturation ◽  
Chloroform Extract ◽  
Aqueous Extracts ◽  
Kaempferia Galanga ◽  
The Family ◽  
Anti Inflammatory ◽  
One Way Anova

Introduction: Kaempferia galanga is a medicinal plant belonging to the family Zingiberaceae: ginger family. It is treated as a folk traditional herb. Anti Inflammatory property refers to the ability of a substance to reduce inflammation or any of its 5 cardinal signs. Aim: To assess and compare in vitro the anti-inflammatory properties of various extracts (ethanol, chloroform and aqueous) of Kaempferia galanga L Rhizome. Materials and Methods: Protein Denaturation Inhibition was carried out in vitro and statistical analysis was done using ONE WAY -ANOVA and Duncan Multiple Range Tests. The test was done in triplicates. Results: Chloroform extract of Kaempferia galanga rhizome has the best anti-inflammatory potential followed by Ethanol and Aqueous extracts of the rhizome. Conclusion: With further in vivo and clinical research, the chloroform, ethanolic and aqueous extracts of Kaempferia galanga can be recommended as a novel, innovative and potent anti-inflammatory drug in the market as it’s natural and doesn’t have side effects.

scholarly journals In vitro and in vivo evaluation of pharmacological potential of Begonia barbata Wall

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Mohammad Rashedul Haque ◽  
Mirazul Islam ◽  
Md. Ruhul Kuddus
Keyword(s):  
Acetic Acid ◽  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Total Phenolic ◽  
Plant Samples ◽  
Tail Immersion ◽  
Thiopental Sodium ◽  
Pharmacological Potential

Abstract Background Begonia barbata Wall is an important medicinal plant in Bangladesh used to treat several human diseases. The intention of the existing work was to determine in vitro and in vivo pharmacological properties of petroleum-ether, dichloromethane, and ethyl acetate soluble fractions of methanol extract of B. barbata whole plant (MEBB). The total phenolic content (TPC) was determined by Folin-Ciocalteu test while antioxidant activity assay was performed by DPPH radical quenching process. Tail immersion and acetic acid-induced writhing methods were utilized to evaluate analgesic effect in albino mice. To evaluate the hypoglycemic potential, glucose tolerance test is used. Castor oil-induced diarrhea method is utilized to figure out the anti-diarrheal action in mice. Thiopental-sodium-induced sleeping time test was exploited for the appraisal of CNS antidepressant action. Results Antioxidant activities of the test materials followed the order: dichloromethane fraction (DCMF) > ethyl acetate fraction (EAF) > petroleum-ether fraction (PEF). The EAF yielded maximum amount of phenolic compounds (12.47 mg of GAE/g of sample). The DMF was most effective in terms of thrombolytic activity while the EAF protected the membrane integrity of erythrocyte by 63.47% inhibition of hemolysis induced by heat. Among all fractions, the PEF (400 mg/kg body weight) demonstrated highest analgesic activity in both tail immersion test (maximum elongations as 262.053%) and acetic acid-induced writhing method (66.02% writhing inhibition). During anti-diarrheal test, the plant samples significantly diminished the frequency of diarrheal episodes in mice. Similarly, the plant samples decreased the sleeping duration induced by thiopental sodium. Conclusion The above outcome exposed that B. barbata possesses significant pharmacological potential, which validates its use in Bangladesh folk medicinal practices.

scholarly journals In-vivo and in-vitro evaluation of pharmacological activities of Ardisia solanacea leaf extract

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Mohammad Rashedul Islam ◽  
Jannatul Naima ◽  
Nawreen Monir Proma ◽  
Md. Saddam Hussain ◽  
S. M. Naim Uddin ◽  
...  
Keyword(s):  
Body Weight ◽  
Leaf Extract ◽  
Elevated Plus Maze ◽  
Open Field Test ◽  
Immersion Method ◽  
Plus Maze ◽  
Tail Immersion ◽  
Hole Board

Abstract Background The Bangladeshi rural and hilly areas people have long tradition to use various medicinal plants for treating different diseases. That’s why, the crude methanolic leaf extract of Ardisia solanacea with its different fractions (petroleum ether, carbon tetrachloride, n-hexane and chloroform fractions) were subjected to investigate bioactivities in swiss albino mice; namely analgesic, CNS, and Oral hypoglycemic activities, while in-vitro evaluation of cytotoxicity. Methods Central nervous system activity was investigated by various method such as Elevated plus maze, Hole board, Hole cross and Open field test apparatus. Analgesic activity was evaluated by both acetic acid induced and tail immersion method. Hypoglycemic activity was evaluated by oral glucose tolerance test and cytotoxicity was evaluated by Brine shrimp lethality bioassay. Results In CNS activity, among others fractions, ASCF fraction produced a significant anxiolytic activity in both elevated plus maze and Hole board test. During open-field test almost all the fractions of A. solanacea leaves extract display decreased locomotor activities that indicates significant sedative activity. The ASME and ASCF showed significant peripheral analgesic activity at a dose of 200 mg/kg and 400 mg/kg body weight (p < 0.05). In tail immersion method, among others extracts chloroform fractions exhibited significant (p < 0.05) elongation of reaction time 30 min after oral dose of 200 and 400 mg/kg body weight respectively. The methanolic and n-hexane extracts reduced blood glucose level significantly after 90 min with value of 53.94% and 48.15% respectively (p < 0.05). In case of cytotoxicity activity, among other fractions carbon tetrachloride fraction showed lowest LC50 values. Conclusions From the above results, it is clear that different fractions of A. solanacea showed significant pharmacological potentiality in different in-vitro and in-vivo study model. So, it will be very much possible source for an isolating lead compound for curing the numerous disorders.

In vitro and in vivo evaluation of the anti-inflammatory effects of Arzanol from Helichrysum italicum

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
J Bauer ◽  
F Dehm ◽  
A Koeberle ◽  
F Pollastro ◽  
G Appendino ◽  
...  
Keyword(s):  
In Vivo Evaluation ◽  
Anti Inflammatory

Haemanthus coccineus extract shows profound anti-inflammatory actions in vitro and in vivo due to its main alkaloid narciclasine

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
S Fuchs ◽  
LT Hsieh ◽  
W Saarberg ◽  
CAJ Erdelmeier ◽  
TA Wichelhaus ◽  
...  
Keyword(s):  
Anti Inflammatory

The Development of Homologous (Canine/Anti-Canine) Antibodies in Dogs with Haemophilia A (Factor VIII Deficiency): A Ten-Year Longitudinal Study

1993 ◽  
Vol 69 (01) ◽  
pp. 021-024 ◽  
Author(s):  
Shawn Tinlin ◽  
Sandra Webster ◽  
Alan R Giles
Keyword(s):  
Factor Viii ◽  
Canine Model ◽  
Significant Inhibition ◽  
Human Condition ◽  
Haemophilia A ◽  
Variable Expression ◽  
The Family ◽  
Over Time

SummaryThe development of inhibitors to factor VIII in patients with haemophilia A remains as a serious complication of replacement therapy. An apparently analogous condition has been described in a canine model of haemophilia A (Giles et al., Blood 1984; 63:451). These animals and their relatives have now been followed for 10 years. The observation that the propensity for inhibitor development was not related to the ancestral factor VIII gene has been confirmed by the demonstration of vertical transmission through three generations of the segment of the family related to a normal (non-carrier) female that was introduced for breeding purposes. Haemophilic animals unrelated to this animal have not developed functionally significant factor VIII inhibitors despite intensive factor VIII replacement. Two animals have shown occasional laboratory evidence of factor VIII inhibition but this has not been translated into clinical significant inhibition in vivo as assessed by clinical response and F.VIII recovery and survival characteristics. Substantial heterogeneity of inhibitor expression both in vitro and in vivo has been observed between animals and in individual animals over time. Spontaneous loss of inhibitors has been observed without any therapies designed to induce tolerance, etc., being instituted. There is also phenotypic evidence of polyclonality of the immune response with variable expression over time in a given animal. These observations may have relevance to the human condition both in determining the pathogenetic factors involved in this condition and in highlighting the heterogeneity of its expression which suggests the need for caution in the interpretation of the outcome of interventions designed to modulate inhibitor activity.

Natural Anti-inflammatory compounds as Drug candidates in Alzheimer’s disease

2020 ◽  
Vol 27 ◽  
Author(s):  
Reyaz Hassan Mir ◽  
Abdul Jalil Shah ◽  
Roohi Mohi-ud-din ◽  
Faheem Hyder Potoo ◽  
Mohd. Akbar Dar ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Natural Products ◽  
Protective Action ◽  
New Drugs ◽  
Huperzine A ◽  
Brain Disorder ◽  
Anti Inflammatory

: Alzheimer's disease (AD) is a chronic neurodegenerative brain disorder characterized by memory impairment, dementia, oxidative stress in elderly people. Currently, only a few drugs are available in the market with various adverse effects. So to develop new drugs with protective action against the disease, research is turning to the identification of plant products as a remedy. Natural compounds with anti-inflammatory activity could be good candidates for developing effective therapeutic strategies. Phytochemicals including Curcumin, Resveratrol, Quercetin, Huperzine-A, Rosmarinic acid, genistein, obovatol, and Oxyresvertarol were reported molecules for the treatment of AD. Several alkaloids such as galantamine, oridonin, glaucocalyxin B, tetrandrine, berberine, anatabine have been shown anti-inflammatory effects in AD models in vitro as well as in-vivo. In conclusion, natural products from plants represent interesting candidates for the treatment of AD. This review highlights the potential of specific compounds from natural products along with their synthetic derivatives to counteract AD in the CNS.

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