BIOSYNTHESIZED SILVER NANOPARTICLES USING CATHARANTHUS ROSEUS AND THEIR ANTIBACTERIAL EFFICACY IN SYNERGY WITH ANTIBIOTICS; A FUTURE ADVANCEMENT IN NANOMEDICINE

2021 ◽  
pp. 116-124
Author(s):  
MONIKA GUPTA
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Catharanthus Roseus ◽  
Leaf Extract ◽  
Research Work ◽  
Resistant Bacteria ◽  
Surface Plasmon Resonance Peak ◽  
Plasmon Resonance Peak ◽  
X Ray ◽  
Uniform Dispersion

Objective: This research work develops an approach to synthesize silver nanoparticles (AgNPs) by reduction of leaf extract of Catharanthus roseus plant. This study produces synthesized nanoparticles that have process-controlled attributes which make their antibiotic action highly efficient. These attributes include smaller size, proper morphology, uniform dispersion, metal ion content, and formation of functional groups. By optimizing the reduction process parameters, AgNPs gain the desired properties.  Methods: The biosynthesis of AgNPs process was performed using reaction of 10% (w/v) C. roseus leaf extract with AgNO3. The optimum conditions and concentration used for synthesis of nanoparticles were: 1 mM AgNO3, pH 5, and temperature 80°C with an incubation time of 72 h. All the above parameters were analyzed by ultraviolet-visible spectrophotometer with the surface plasmon resonance peak obtained at 440 nm. Results: Various characterization techniques were performed, namely, scanning electron microscopy, energy-dispersive X-ray, transmission electron microscopy, photoluminescence study, X-ray diffraction spectroscopy, Fourier transform infrared, dynamic light scattering, and atomic force microscopy. The results obtained from characterization confirmed the spherical morphology of the nanoparticles with size between 50 and 87 nm. In the current investigation, the antimicrobial activity of biosynthesized AgNPs was also determined using minimum inhibitory concentration and zone of inhibition methods against six different bacteria at different doses of AgNPs (100, 150, and 200 μg/ml) alone and also in combination with antibiotic-streptomycin. Conclusion: The results revealed that high concentration of AgNPs inhibits the bacterial growth. Furthermore, AgNPs revealed much stronger antibacterial action in synergy with streptomycin against antibiotic-resistant bacteria.

scholarly journals Phytochemical Mediated Synthesis of Silver Nanoparticles using Musa paradisiaca Peduncle Latex and Its Photocatalytic and Antimicrobial Activity

2018 ◽  
Vol 4 (5) ◽  
pp. 503-507
Author(s):  
B. Venkataramana ◽  
S. Siva Sankar ◽  
A. Saikumar ◽  
B. Vijaya Kumar Naidu
Keyword(s):  
Electron Microscopy ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Bacterial Species ◽  
Spherical Shape ◽  
Blue Dye ◽  
Surface Plasmon Resonance Peak ◽  
Plasmon Resonance Peak ◽  
X Ray ◽  
Musa Paradisiaca

In the present work, green synthesis of silver nanoparticles (AgNPs) using a natural phytochemical agent has been described. The aqueous latex from Musa paradisiaca peduncle has been utilized as reducing as well as stabilizing agent. The formation of AgNPs was optimized by varying latex and AgNO3 concentrations and finally reaction time. Ultraviolet-visible spectroscopic analysis showed the surface plasmon resonance peak between 350 and 450 nm confirms the formation of silver nanoparticles. X-ray powder diffraction analysis revealed the crystalline nature of AgNPs, Fourier transform infrared spectroscopy analysis revealed that AgNPs were stabilized by polyphenols and other aromatics present in the Musa Paradisiaca peduncle latex, while X-ray energy dispersive spectroscopy confirms the metallic nature. The field emission scanning electron microscopy and high resolution transmission electron microscopy showed the spherical shape of the particles and size distribution of AgNPs measured by dynamic light scattering which are in the range of 40 to 50 nm. The synthesized AgNPs showed photocatalytic activity on the degradation / removal of the methylene blue dye and the antimicrobial activity against Pseudomonas aeruginosa, Staphylococcus aureus and Klebseilla bacterial species.

scholarly journals PSIDIUM GUAJAVA: A NOVEL PLANT IN THE SYNTHESIS OF SILVER NANOPARTICLES FOR BIOMEDICAL APPLICATIONS.

2018 ◽  
Vol 11 (1) ◽  
pp. 341
Author(s):  
Sharmila C ◽  
Ranjith Kumar R ◽  
Chandar Shekar B
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Biomedical Applications ◽  
Leaf Extract ◽  
Cost Effective ◽  
Psidium Guajava ◽  
Tem Analysis ◽  
X Ray ◽  
Green Route

 Objective: Synthesis of silver nanoparticles (AgNPs) using a simple, cost-effective and environmentally friendly green route approach and to study the antibacterial activity of AgNPs against human pathogens.Methods: Green route approach is used to synthesize AgNPs using Psidium guajava leaf extract. Fourier transform infrared (FTIR) was used to identify the presence of the functional group. X-ray diffraction (XRD) was used to analyze the structure of prepared AgNPs. Energy dispersive X-ray was used to the characteristic to the composition of the prepared nanoparticles. Size and morphology of the prepared AgNPs were investigated using field emission scanning electron microscopy (FESEM) and transmission electron microscopy (TEM) analysis. Antibacterials efficiency of prepared AgNPs was tested against Escherichia coli and Staphylococcus aureus by well diffusion methods.Results: FTIR study shows the presence of different functional groups present in the leaves mediated AgNPs. The XRD studies yield diffraction peaks corresponding to face-centered cubic structure of Ag crystals. Spherical shaped AgNPs with a particle size of about ~55 nm were evidenced using FESEM and TEM analysis. Energy dispersive spectrum of the synthesized AgNPs confirms the presence of silver in the prepared nanoparticles. From UV-VIS analysis it is shown that the absorption band was red-shifted from 430 nm to 456 nm. The prepared AgNPs shows good antibacterial activity against E. coli and S. aureus.Conclusions: P. guajava leaf extract is a potential reducing agent to synthesize AgNPs. The green synthesis approach provides cost-effective and eco-friendly nanoparticles, which could be used in biomedical applications.

Analysing the phytochemistry and anti-oxidant property of fabricated silver nanoparticles using Catharanthus roseus leaf extract

2021 ◽  
Vol 16 (12) ◽  
pp. 72-79
Author(s):  
A.K. Keshari ◽  
S. Saxena ◽  
G. Pal ◽  
V. Srivashtav ◽  
R. Srivastav
Keyword(s):  
Silver Nanoparticles ◽  
Electron Microscope ◽  
Catharanthus Roseus ◽  
Leaf Extract ◽  
Spherical Shape ◽  
Silver Ions ◽  
Antioxidant Property ◽  
X Ray ◽  
Morphological Studies ◽  
Aqueous Leaf Extract

A green rapid biogenic synthesis of silver nanoparticles (AgNPs) using Catharanthus roseus leaf extract was performed. Synthesized nanoparticles were characterized using UV-Visible, Fourier transform infra-red (FTIR) and X-ray diffraction (XRD). The reduction of silver ions to AgNPs using C. roseus extract was completed within 240 mins. The formation of AgNPs was confirmed by Surface Plasmon Resonance (SPR) at 442 nm using UV-Vis Spectrophotometer and it is characterized by XRD, Transmission electron microscope (TEM) and Scanning electron microscope (SEM). The morphological studies revealed the spherical shape of the particles with sizes ranging from 16-35 nm and Energy dispersive X-ray (EDX) spectrum confirmed the presence of silver along with other elements in the plants metabolite. The extracellular AgNPs synthesis by aqueous leaf extract demonstrates ultra-fast, simple and inexpensive method comparable to other methods. The antioxidant assay of the synthesized AgNPs indicated that they have a strong antioxidant property as compared to the control. Since these compounds are also safe to use and discharged into the environment, the green AgNPs could be considered as an innovative alternative approach for biomedical and nanoscience based industries.

scholarly journals Star-Shaped Poly(2-ethyl-2-oxazoline) and Poly(2-isopropyl-2-oxazoline) with Central Thiacalix[4]Arene Fragments: Reduction and Stabilization of Silver Nanoparticles

Polymers ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 2006 ◽  
Author(s):  
Alexey Lezov ◽  
Alexander Gubarev ◽  
Maria Mikhailova ◽  
Alexandra Lezova ◽  
Nina Mikusheva ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Resonance Peak ◽  
Time Dependent ◽  
Colloidal Solutions ◽  
X Ray Diffraction ◽  
Plasmon Resonance Peak ◽  
X Ray ◽  
Transmission Electron ◽  
Kinetics Of

The interaction of silver nitrate with star-shaped poly(2-ethyl-2-oxazoline) and poly(2-isopropyl-2-oxazoline) containing central thiacalix[4]arene cores, which proceeds under visible light in aqueous solutions at ambient temperature, was studied. It was found that this process led to the formation of stable colloidal solutions of silver nanoparticles. The kinetics of the formation of the nanoparticles was investigated by the observation of a time-dependent increase in the intensity of the plasmon resonance peak that is related to the nanoparticles and appears in the range of 400 to 700 nm. According to the data of electron and X-ray spectroscopy, scanning and transmission electron microscopy, X-ray diffraction analysis, and dynamic light scattering, the radius of the obtained silver nanoparticles is equal to 30 nm. In addition, the flow birefringence experiments showed that solutions of nanoparticles have high optical shear coefficients.

scholarly journals Green Synthesis of Silver Nanoparticles using Strobilanthes flaccidifolius Nees. Leaf Extract and its Antibacterial Activity

2016 ◽  
Vol 8 (1) ◽  
pp. 1523-1532 ◽  
Author(s):  
Sujata D Wangkheirakpam ◽  
Wangkheirakpam Radhapiyari Devi ◽  
Chingakham Brajakishore Singh ◽  
Warjeet S Laitonjam
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
X Ray Diffraction ◽  
Paramagnetic Resonance ◽  
X Ray ◽  
Vis Spectroscopy ◽  
Electron Paramagnetic Resonance Technique ◽  
Average Size ◽  
Green Technique

The leaf extract of Strobilanthes flaccidifolius Nees. was used for the synthesis of silver nanoparticles through a green technique of synthesis. The nanoparticles was characterized by UV-VIS spectroscopy which proves the formation silver nanoparticles. FTIR (Fourier Transmission infra red spectroscopy) study was carried out to assess the biomolecule as indigo precursors, Energy dispersion X-ray analysis(EDX) data further proves it. EPR (Electron paramagnetic resonance technique) shows the free radical in silver neutral state and XRD(X-ray diffraction technique) also repots silver neutral formation.The morphology and the shape of the silver nanoparticles were determined by Scanning electron microscopy(SEM) and Tunneling electron microscopy (TEM).The nanoparticles adopted spherical morphology and the size ranging from 6nm to 54.11nm and average size was determined as 12.15± 5.3nm.The nanoparticles had antimicrobial activity

Preparation of Silver Nanoparticles in Poly(N-vinylpyrrolidone)/Ethanol Solutions

2017 ◽  
Vol 16 (05n06) ◽  
pp. 1750008 ◽  
Author(s):  
Shu Zhang ◽  
Yongan Tang ◽  
Branislav Vlahovic
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Silver Nitrate ◽  
Weight Ratio ◽  
Plasmon Resonance Peak ◽  
X Ray ◽  
Transmission Electron ◽  
Particle Size And Morphology ◽  
Size And Morphology ◽  
Average Size

Silver nanoparticles have been synthesized from Poly(N-vinylpyrrolidone) (PVP)/ethanol solutions, and six different PVP to silver nitrate weight ratios (PVP:AgNO3) are studied in reduction of silver nitrate in ethanol with the presence of PVP as a stabilizer. The produced silver nanoparticles showed strong plasmon resonance peak centered at around 405[Formula: see text]nm in UV-Vis spectra. The particle morphologies were also examined and compared under secondary electron microscopy (SEM) and transmission electron microscopy (TEM). Energy dispersion X-ray spectroscopy (EDX) was utilized to determine the formation of silver nanoparticles. We found that the particle size and morphology were strongly dependent on the PVP:AgNO3 weight ratio. The average size of silver particles decreased from 19.25[Formula: see text]nm to 10.03[Formula: see text]nm as the weight ratio of PVP:AgNO3 increased from 1:1 to 20:1.

scholarly journals Síntesis de nanopartículas de plata reducidas con desecho producido por el insecto Ulomoides dermestoides

2019 ◽  
pp. 29-36
Author(s):  
Isabel Ramos-Villa ◽  
Diego Alberto Lomelí-Rosales ◽  
Sandra Fabiola Velasco-Ramírez ◽  
Gilberto Velazquez-Juarez
Keyword(s):  
Electron Microscopy ◽  
Amino Acids ◽  
Silver Nanoparticles ◽  
Colloidal Solution ◽  
Surface Plasmon Resonance Peak ◽  
Plasmon Resonance Peak ◽  
Transmission Electron ◽  
The Family ◽  
Darkling Beetles ◽  
Shape Of Nanoparticles

The scale of nanotechnology goes from 0.2 nm to approximately 100 nm and is used in many different disciplines. U.dermestoides is a beetle belonging to the family Tenebrionidae,commonly known as darkling beetles, it has phytophagous habits. In its waste have been identified the following organic compounds: Saponins, coumarins, steroids, phenols, alkaloids, proteins and amino acids. The synthesis of silver nanoparticles was done using colloidal solution from the reduction of silver nitrate at different concentrations, using U. dermestoides waste from three different diets: Rye and Apple; oats, bread and Apple and finally bread and Apple. Series of qualitative tests were done to determinate the presence of phenols, alkaloids carbohydrates, proteins and amino acids. UV-vis spectrophotometry was used in a range from 200 to 800 nm. The results show that between 400-450 nm there is a surface plasmon resonance peak, indicating the formation of silver nanoparticles. By scanning electron microscopy (SEM) the presence of spherical silver nanoparticles was observed and by transmission electron microscopy (TEM) was confirmed the presence and shape of nanoparticles.

scholarly journals Green Synthesis, Characterization and Antibacterial Activity of Silver Nanoparticles from Capparis Spinosa Leaf Extract

2021 ◽  
Vol 72 (1) ◽  
pp. 145-152
Author(s):  
Saleh H. Salmen ◽  
Eman Damra ◽  
Tahani Awad Alahmadi ◽  
Sulaiman Ali Alharbi
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Green Synthesis ◽  
Plant Extract ◽  
Leaf Extract ◽  
Average Particle ◽  
Surface Plasmon Resonance Peak ◽  
Plasmon Resonance Peak ◽  
E Coli ◽  
Capparis Spinosa

The current study reports the green synthesis of silver nanoparticles (AgNPs) using Capparis spinosa leaf extract acting as a capping and reducing agent. The characterization of AgNPs was confirmed using ultraviolet-visible spectrophotometry (UV-Visible), fourier transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). The plant extract used reduces Ag+ into AgNPs within a few minutes as indicated by the changed color, from yellow to reddish-brown. The UV-vis spectrum of AgNPs appeared a characteristic surface plasmon resonance peak at 400-450 nm. FTIR spectroscopy confirmed the role of plant extract as a reducing and capping agent of silver ions. The spectra of FTIR revealed a broad transmission peaks from 3412 to 617 cm-1. An EDX analysis signal at 3 keV and weight 65.38% showed the peak to be in the silver region, a fact which was confirmed by the presence of elemental silver. Under TEM, the nanoparticles were seen to be spherical, with an average particle size of 13 nm. AgNPs showed antibacterial activity against S.epidermidis, S. aureus, MRSA and E. coli. The inhibition zones for S.epidermidis and S. aureus were 8 to 10 mm, while MRSA is 7 to 10 mm. The inhibition zone of E. coli was higher at 10 to 13 mm.

scholarly journals Potential of Topical Cosmeceuticals Cream Functionalized with Biogenic Metallic Nanostructured Material as Antibacterial Agents

2021 ◽  
pp. 80-96
Author(s):  
Rajesh Dodiya ◽  
Mrunal K Shirsat ◽  
Jitendra K Patel
Keyword(s):  
Electron Microscopy ◽  
Silver Nanoparticles ◽  
Silver Nanoparticle ◽  
Eucalyptus Globulus ◽  
Leaf Extract ◽  
Main Element ◽  
Diffusion Method ◽  
Dependent Manner ◽  
X Ray ◽  
Aqueous Leaf Extract

Aims: In this study, the phytochemical analysis of Eucalyptus globulus leaf was analyzed and  used in synthesis of silver nanoparticles. The silver nanoparticle incorporated antimicrobial cosmeceutical cream was developed and characterized for physicochemical parameters, antimicrobial properties, and biocompatibility was evaluated. Methodology: E. globulus aqueous leaf extract was preliminary analyzed for the presence of phytochemical and confirmed using thin layer chromatography techniques. Further, a green synthesis of silver nanoparticle was accomplished using aqueous leaf extract of E. globulus. The formation of nanoparticles was confirmed and characterized by UV-vis spectrophotometer, transmission electron microscopy, dynamic light scattering, zeta potential, X-ray diffractometer, field emission scanning electron microscopy, and fourier transform infrared spectroscopy. The nanoparticles were incorporated in cream and the antimicrobial property was evaluated using agar well diffusion method. Results: The phyto-chemical evaluation of E. globulus aqueous leaf extract showed the presence of phenolic, tannins, saponnins, carbohydrate, and glycoside. Moreover, Eucalyptus globulus aqueous leaf extract exhibited antioxidant activity in a dose dependent manner. The surface plasmon resonance peak was 424 nm and functional group such as hydroxyl, carboxyl, alkyl halides, amines, carbonyl, amide groups, and phenolic compounds were present which was important for the bio-reduction, stabilization, and capping of the silver nitrate into nanoparticles. Energy dispersive x-ray (EDX) analysis showed silver as the main element present and the nanoparticles were oval in shape and 19-60 nm in size with effective diameter of 90 nm. The test cream exhibited surface roughness of ≈ 30 nm, contact angle of ≈ 100, and surface energy of ≈88 mN/m. The formulated creams were consistent, with satisfactory pH, viscosity and spreadability. Conclusion: The results demonstrated an eco-friendly and cost-effective approach to synthesis biogenic silver nanoparticles using aqueous extract of E. globulus. Eucalyptus globulus aqueous leaf extract stabilized and capped silver nanoparticles incorporated topical cream exhibited potent antimicrobial efficacy against Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa.

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