scholarly journals DURATION DEPENDENT ANTIAPOPTOTIC EFFICACY OF CAMELLIA SINENSIS (GREEN TEA) ON STREPTOZOTOCIN INDUCED DIABETES LINKED TESTICULAR HYPOFUNCTION IN ALBINO RAT: GENOMIC AND FLOW CYTOMETRIC ASSESSMENT

2019 ◽  
pp. 85-93
Author(s):  
BARNALI DAS ◽  
BAISAKHI BISWAS ◽  
Debidas Ghosh
Keyword(s):  
Camellia Sinensis ◽  
Thiobarbituric Acid ◽  
Serum Testosterone ◽  
Ethyl Acetate Fraction ◽  
Diabetic Rat ◽  
Duration Of Treatment ◽  
Albino Rat ◽  
Significant Correction ◽  
Testicular Impairment ◽  
Threshold Duration

Objective: The study has been conducted to search out the threshold duration of treatment of ethyl acetate fraction of methanolic extract of leaves of Camellia sinensis (L.) Kuntze at the dose of 100 mg/kg body weight for the management of diabetes-induced testicular impairment in streptozotocin-induced diabetic rat in a duration dependent fashion. Methods: In this respect, the glycemic, androgenic, oxidative stress sensors, gene expression of testicular androgenic key enzymes along with apoptotic markers were evaluated in a duration dependent way (14, 28 and 56 d). Results: A significant correction was noted in the levels of glycated haemoglobin (HbA1C), testicular thiobarbituric acid reactive substances (TBARS), conjugated diene (CD), sperm viability, sperm mitochondrial status, serum testosterone, and genomic expression of testicular Δ5, 3β-hydroxysteroid dehydrogenase (HSD), 17β-HSD, Bax, Bcl-2 after treatment for different duration with the said fraction in diabetic groups in compare to respective vehicle-treated diabetic group without any toxicity induction in general. Thin layer chromatography (TLC) study of the fraction showed two spots with retention factors (Rf) of 0.78 and 0.51. Conclusion: The results showed that 28 d treatment was threshold duration of treatment for the correction of diabetes-induced testicular impairment.

scholarly journals Antidiabetic Potentiality of the Aqueous-Methanolic Extract of Seed ofSwietenia mahagoni(L.) Jacq. in Streptozotocin-Induced Diabetic Male Albino Rat: A Correlative and Evidence-Based Approach with Antioxidative and Antihyperlipidemic Activities

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Debasis De ◽  
Kausik Chatterjee ◽  
Kazi Monjur Ali ◽  
Tushar Kanti Bera ◽  
Debidas Ghosh
Keyword(s):  
Control Level ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Seed Extract ◽  
Diabetic Rat ◽  
Thiobarbituric Acid Reactive Substances ◽  
Albino Rat ◽  
Diabetic Model ◽  
Catalase Peroxidase ◽  
Swietenia Mahagoni

Antidiabetic, antioxidative, and antihyperlipidemic activities of aqueous-methanolic (2 : 3) extract ofSwietenia mahagoni(L.) Jacq. (family Meliaceae) seed studied in streptozotocin-induced diabetic rats. Feeding with seed extract (25 mg 0.25 mL distilledwater−1100 gm b.w.−1rat−1 day−1) for 21 days to diabetic rat lowered the blood glucose level as well as the glycogen level in liver. Moreover, activities of antioxidant enzymes like catalase, peroxidase, and levels of the products of free radicals like conjugated diene and thiobarbituric acid reactive substances in liver, kidney, and skeletal muscles were corrected towards the control after this extract treatment in this model. Furthermore, the seed extract corrected the levels of serum urea, uric acid, creatinine, cholesterol, triglyceride, and lipoproteins towards the control level in this experimental diabetic model. The results indicated the potentiality of the extract ofS. mahagoniseed for the correction of diabetes and its related complications like oxidative stress and hyperlipidemia. The extract may be a good candidate for developing a safety, tolerable, and promising neutraceutical treatment for the management of diabetes.

scholarly journals Evaluation of antidiabetic and antioxidative efficacy of ethyl acetate fraction of methanolic extract of Camellia sinensis (green tea) leaves in streptozotocin induced diabetic albino rat

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Baisakhi Biswas ◽  
Barnali Das ◽  
Kishalay Jana ◽  
Debidas Ghosh
Keyword(s):  
Ethyl Acetate ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Methanolic Extract ◽  
Fasting Blood Glucose ◽  
Ethyl Acetate Fraction ◽  
Diabetic Rat ◽  
Hepatic Tissue ◽  
Tea Leaves ◽  
Green Tea Leaves

<p><strong>Objective:</strong> To investigate the antidiabetic and antioxidative potentiality of ethyl-acetate fraction of methanolic extract of <em>Camellia sinensis</em> (Green tea) leaves in streptozotocin induced diabetic rat. <strong>Methods:</strong> Streptozotocin induced diabetic state was confirmed by increased level of fasting blood glucose, decreased level of serum insulin along with inhibition in carbohydrate metabolomics. Oxidative stress was assessed by measuring antioxidative enzyme activities of hepatic and skeleto-muscular tissue. Hepatic <em>Hexokinase-I</em>, pro-apoptotic <em>Bax</em> and anti-apoptotic <em>Bcl-2</em> gene expression patterns were noted by qRT-PCR technique. <strong>Results:</strong> After treatment with ethyl-acetate fraction of methanolic extract of <em>Camellia sinensis</em> (Green tea) leaves at a dose of 100 mg/kg body weight/day to diabetic rats for 28 days, a significant (p &lt; 0.05) recovery was noted in fasting blood glucose level, serum insulin level along with activities of carbohydrate metabolic enzymes in hepatic tissue in respect to the vehicle treated diabetic group. This fraction also resulted a significant (p &lt; 0.05) recovery in the activities of antioxidative enzymes in hepatic and skeleto - mascular tissue. In streptozotocin induced diabetic rat the low level of expression of <em>Hexokinase-I</em>, anti-apoptotic <em>Bcl-2</em> and high level of expression of pro-apoptotic <em>Bax</em> gene were observed in hepatic tissue in respect to vehicle treated control. There were recovered significantly after the treatment with the said fraction. <strong>Conclusion: </strong>From the results, it may be concluded that ethyl-acetate fraction of methanolic extract of leaves of <em>C. sinensis</em> has a promising anti-diabetic and antioxidative activities for the management of streptozotocin induced diabetic state.</p>

Ameliorative role of ethyl-acetate fraction of methanolic leaf extract of Camellia sinensis (green tea) on streptozotocin-induced diabetes linked testicular hypofunction in albino rat: A dose-dependent biochemical and genomic transection study

2017 ◽  
Vol 14 (4) ◽  
Author(s):  
Barnali Das ◽  
Baisakhi Biswas ◽  
Abhinandan Ghosh ◽  
Bhabani Prasad Pakhira ◽  
Debidas Ghosh
Keyword(s):  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Fasting Blood Glucose ◽  
Ethyl Acetate Fraction ◽  
Toxicity Assessment ◽  
Diabetic Group ◽  
Diabetic Rat ◽  
Albino Rat ◽  
Dose Dependent Fashion ◽  
Dose Dependent

AbstractBackgroundThe present investigation focuses the diabetes-induced testicular hypofunction and its possible correction by the effective dose of ethyl-acetate fraction of methanolic extract ofMethodsThe androgenic, spermiological, oxidative stress and apoptosis sensors along with testicular genomic sensors were evaluated in a dose-dependent fashion (50 mg or 100 mg or 200 mg/kg body weight). Activities of hepatic transaminases for toxicity assessment were also measured.ResultsIncreased level of fasting blood glucose, testicular cholesterol, seminal vesicular fructose along with a low count, motility and viability of epididymal sperm, low activities of testicular Δ5, 3β-hydroxy steroid dehydrogenase (HSD), 17β-HSD, testicular antioxidant enzymes (catalase and superoxide dismutase) and low plasma level of testosterone were noted in diabetic rat in respect to the control. After oral administration of said fraction to diabetic rat, levels of above sensors were resettled toward the control. A significant decrease in the number of different generations of germ cells at the stage VII of spermatogenesis in diabetic rat was noted which were recovered significantly toward the control in the fraction-treated diabetic group. It was supported by the correction in gene expression of testicular Δ5, 3β- HSD, 17β- HSD, Bcl-2 and Bax in the fraction-treated diabetic group.ConclusionsThe threshold dose of ethyl-acetate fraction of methanolic extract of

scholarly journals Antioxidant and antibacterial activity of extracts, fractions and isolated substances from the flowers of Acacia podalyriifolia A. Cunn. ex G. Don

2010 ◽  
Vol 46 (4) ◽  
pp. 715-722 ◽  
Author(s):  
Cláudia Alexandra de Andrade ◽  
João Luiz de Souza Carvalho ◽  
Miriam Machado Cunico ◽  
Ana Luísa Lacava Lordello ◽  
Carmen Etsuko Kataoka Higaskino ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Thiobarbituric Acid ◽  
Ethyl Acetate Fraction ◽  
Total Phenol Content

The extracts and fractions from the flowers of A. podalyriifolia were analyzed previously for antibacterial activity using diffusion in disk, Antioxidant properties were evaluated by determining radical scavenging power (DPPH test) and total phenol content was measured (Folin method). The present study describes the in vitro antibacterial (determining minimum inhibitory concentration) and antioxidant activities (by thiobarbituric acid reactive species - TBARS method) for the ethanol extract, dichloromethane and ethyl acetate fractions and two flavanones (naringenin and 5-β-D-glycosyl-naringenin) isolated from the flowers of Acacia podalyriifolia A. Cunn. ex G. Don. The flavanones naringenin and 5-β-D-glycosyl-naringenin had not previously been obtained from this species. The most effective antibacterial activity was observed in the ethyl acetate fraction (MIC=0.25 mg mL-1 against Staphylococcus aureus ATCC 6538, MIC = 0.125 mg mL-1 against Staphylococcus epidermidis ATCC 12229, MIC=0.5 mg mL-1 against Streptococcus pyogenes ATCC 19615, Klebsiella pneumoniae ATCC 13883 and Proteus mirabilis ATCC 43071). The evaluated samples showed antioxidant activity on the TBARS test, especially for ethanol extract (1000 ppm), which was the most active (29.43% ± 0.65) followed by ethyl acetate fraction (1000 ppm, 24.84% ± 1,28), both demonstrating higher activity than that presented by ascorbic acid (1000 ppm, 21.73% ± 1.77), although lower than the BHT (1000 ppm 35.15% ± 3.42), both reference compounds. Naringenin and 5-β-D-glycosyl-naringenin demonstrated antioxidant action, but only naringenin inhibited the growth of gram-positive and gram-negative bacteria.

Testicular antioxidants and testosterone enhancing effects of the hydro-ethanolic extract of Rauvolfia vomitoria (Apocynacaea) in male Wistar rats

2021 ◽  
Vol 4 (2) ◽  
pp. 1-6
Author(s):  
Antoine K. Sanda ◽  
Emmanuel Aneng ◽  
Mariama Mbouh ◽  
Faustin P. T. Manfo ◽  
Stanley N. Ngimgoh ◽  
...  
Keyword(s):  
Plant Extract ◽  
Ethanolic Extract ◽  
Thiobarbituric Acid ◽  
Serum Testosterone ◽  
Reproductive Organs ◽  
Male Rats ◽  
Alcoholic Extract ◽  
Traditional Use ◽  
Male Wistar Rats ◽  
Rauvolfia Vomitoria

Abstract Background: Natural products such as extracts of plants have been seen as a possible alternative to conventional therapies in the treatment and management of male infertility. This study aimed at investigating the testicular antioxidant and testosterone enhancing ability of the hydro-alcoholic extract of Rauvolfia vomitoria in male rats. Methods: Twenty-four male rats were divided into four groups of 6 rats each, treated daily with either vehicle (distilled water; 5 mL/kg) or hydro-ethanolic extract of R. vomitoria (20 mg/kg, 40 mg/kg, and 100mg/kg) for 60 days, and body weights recorded once every three days. At the end of the treatment, each animal was sacrificed, and reproductive organs were dissected out and weighed. Serum from capillary blood was used for testosterone quantification, while testicular homogenates were used for the estimation of antioxidant biomarkers. Results: Treatment with R. vomitoria extract did not alter the animal’s body weight. Instead, the extract at the dose of 40 mg/kg significantly increased (P< 0.05) the weights of all reproductive organs investigated. The plant extract also increased serum testosterone concentrations significantly (P< 0.05), with the highest effect observed in the animals treated with a dose of 40 mg/kg. Testicular antioxidant markers, thiobarbituric acid substances, glutathione, and catalase were equally improved (P< 0.05) by treatment with the plant extract at the dose of 40 mg/kg. Conclusion: Hydro-ethanolic extract of R. vomitoria portrayed beneficial pharmacological properties on reproductive organs, testicular antioxidants, and testosterone concentrations in male rats. These pharmacological activities support the traditional use of the plant in the management of male fertility disorders.

Hormonal changes with anastrozole treatment in breast cancer

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e12026-e12026
Author(s):  
S. Ali ◽  
M. F. Ali ◽  
M. Mundia ◽  
A. G. Mundia
Keyword(s):  
Breast Cancer ◽  
Side Effects ◽  
Postmenopausal Women ◽  
Hot Flashes ◽  
Serum Testosterone ◽  
Hormonal Changes ◽  
Duration Of Treatment ◽  
Hormone Receptor Positive ◽  
Hormonal Levels ◽  
Positive Breast Cancer

e12026 Background: Aromatase inhibitors are a class of drugs that inhibit tumor growth in hormone receptor positive breast cancer, in postmenopausal women. They inhibit the conversion of androgens to estrogens. Both clinical benefit and side effects are believed to be due to alterations in blood hormonal levels. Methods: Clinical charts of postmenopausal women with estrogen receptor positive breast cancer, receiving anastrozole 1 mg daily, were reviewed to determine the clinical side effects and blood hormonal changes during treatments, at a community oncology practice. Hormonal levels measured were serum testosterone, serum estrogens and prolactin levels. Results: There were 31 postmenopausal women age 52–73 (median 62) years, receiving anastrozole 1 mg daily. Duration of treatment was 8 to 42 (median 24) months. Clinical side effects noted were joint pains in 4 patients; mild to moderate hot flashes in 4; temporary nausea lasting for 1 week in 3; and vaginal dryness in 2 patients. Serum estrogen levels dropped by 20% in 10 patients from baseline but remained within normal range (14–103 pg/mL), there were no changes in serum testosterone (range 14–76 ng/mL) or prolactin (2.8–29 ng/ml) levels. 2 out of 4 patients with joint pains had testosterone levels in upper range, and remained unchanged during treatments. Conclusions: Anastrozole 1 mg daily is well tolerated in clinical practice, in terms of side effects and does not cause significant blood hormonal changes. No significant financial relationships to disclose.

Corrective role ofEugenia jambolanaon testicular impairment in streptozotocin-induced diabetic male albino rat: An approach through genomic and proteomic study

Andrologia ◽  
2013 ◽  
Vol 46 (3) ◽  
pp. 296-307 ◽  
Author(s):  
A. Ghosh ◽  
K. Jana ◽  
K. M. Ali ◽  
D. De ◽  
K. Chatterjee ◽  
...  
Keyword(s):  
Albino Rat ◽  
Proteomic Study ◽  
Testicular Impairment

Effects of damiana (Turnera diffusa; var. diffusa and var. aphrodisiaca) on diabetic rats

2019 ◽  
Vol 28 (6) ◽  
pp. 84-92
Author(s):  
Edgar Romualdo Esquivel-Gutiérrez ◽  
Lilia Alcaraz-Meléndez ◽  
Roberto Hernández-Herrera ◽  
Amada Torres ◽  
Carmen Rodríguez-Jaramillo
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Biochemical Parameters ◽  
Body Weight Loss ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Glucose Levels ◽  
Turnera Diffusa ◽  
Ethanol Extracts

Turnera diffusa (damiana) grows wild in Baja California Sur (BCS), Mexico. It is traditionally used for its aphrodisiac effects and for diabetes treatment. Two varieties (var.) have been identified: diffusa and aphrodisiaca. To evaluate the effects of water-ethanol extracts from both varieties, measurements on blood glucose, body weight, biochemical parameters, and cell blood counts were performed in a diabetic rat model. Oxidative stress was also evaluated when var. diffusa was administered. No significant reductions in blood glucose levels, in biochemical parameters, or in cell blood counts with any variety of damiana were observed; however, it helped diabetic animals to keep their body weight. Damiana var. diffusa did not modify the mitochondrial nitric oxide levels and superoxide dismutase activity; however, thiobarbituric acid reactive species diminished. In conclusion, damiana can be utilized reliably in the management of diabetes because of its antioxidant potential; it promoted body weight loss in healthy animals and helped to preserve it in diabetes.

scholarly journals PROTECTING OUTCOME OF ETHYL ACETATE FRACTION OF AQUEOUS-METHANOL: 40:60 EXTRACT OF SEED OF MYRISTICA FRAGRANS HOUTT. IN STREPTOZOTOCIN-INDUCED DIABETIC RAT: A DOSE DEPENDENT STUDY

2018 ◽  
Vol 10 (9) ◽  
pp. 54
Author(s):  
Ankita Dey ◽  
Adrija Tripathy ◽  
Debidas Ghosh
Keyword(s):  
Serum Insulin ◽  
Ethyl Acetate Fraction ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Aqueous Methanol ◽  
Myristica Fragrans ◽  
End Products ◽  
Myristica Fragrans Houtt ◽  
Dose Dependent ◽  
Different Doses

Objective: The investigation has been conducted to find out the threshold dose of ethyl acetate fraction prepared from aqueous-methanol (40:60) extract of seed of Myristica fragrans (Houtt.) from the dose dependent experiment by noting the remedial effects of the said fraction on different complications developed in streptozotocin induced diabetic rat.Methods: Diabetic condition was made by single intramascular injection of streptozotocin at a dose of 4 mg/0.1 ml citrate buffer/100 g body weight. Treatment was conducted at different doses for 28 d on diabetic rat. Efficacy of the fraction on fasting blood glucose (FBG) and serum insulin levels, activities of key carbohydrate metabolic enzymes such as hexokinase, glucose-6-phosphate dehydogenase, glucose-6-phosphatase of hepatic and skeletomuscular tissue as well as antioxidative enzymes like catalase, superoxide dismutase (SOD) in connection with the levels of oxidative stress end products such as thiobarbituric acid reactive substances (TBARS), conjugated diene (CD) in hepatic and renal tissue and gene expression of hexokinase-1, SOD, Bax, Bcl-2 in hepatic tissue were assessed.Results: After different doses of fraction treatment to the diabetic animals; FBG, serum insulin levels and activities of carbohydrate metabolic enzymes i.e. hexokinase, glucose-6-phosphate dehydrogenase and glucose-6-phosphatase were rectified towards the control at the level of p<0.05. There is a significant (p<0.05) recovery in the activities of antioxidant enzymes such as catalase and SOD in respect to the vehicle treated diabetic group. Oxidative stress end products such as CD and TBARS levels were increased significantly (p<0.05) in vehicle treated diabetic rats and was rectified significantly (p<0.05) after fraction treatment at the dose of 10 mg or 20 mg or 40 mg fraction treated group. Gene expression of hexokinase-1, superoxide dismutase, bax and bcl-2 was rectified towards vehicle treated control significantly at the level of p<0.05 after treatment of different doses of fractions to the diabetic animals.Conclusion: The study indicates that the seeds of Myristica fragrans at the dose of 20 mg/100 g body weight possess most effective outcome to manage diabetic disorders in streptozotocin induced diabetic rats.

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