scholarly journals Antidiabetic Potentiality of the Aqueous-Methanolic Extract of Seed ofSwietenia mahagoni(L.) Jacq. in Streptozotocin-Induced Diabetic Male Albino Rat: A Correlative and Evidence-Based Approach with Antioxidative and Antihyperlipidemic Activities

2011 ◽  
Vol 2011 ◽  
pp. 1-11 ◽  
Author(s):  
Debasis De ◽  
Kausik Chatterjee ◽  
Kazi Monjur Ali ◽  
Tushar Kanti Bera ◽  
Debidas Ghosh
Keyword(s):  
Control Level ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Seed Extract ◽  
Diabetic Rat ◽  
Thiobarbituric Acid Reactive Substances ◽  
Albino Rat ◽  
Diabetic Model ◽  
Catalase Peroxidase ◽  
Swietenia Mahagoni

Antidiabetic, antioxidative, and antihyperlipidemic activities of aqueous-methanolic (2 : 3) extract ofSwietenia mahagoni(L.) Jacq. (family Meliaceae) seed studied in streptozotocin-induced diabetic rats. Feeding with seed extract (25 mg 0.25 mL distilledwater−1100 gm b.w.−1rat−1 day−1) for 21 days to diabetic rat lowered the blood glucose level as well as the glycogen level in liver. Moreover, activities of antioxidant enzymes like catalase, peroxidase, and levels of the products of free radicals like conjugated diene and thiobarbituric acid reactive substances in liver, kidney, and skeletal muscles were corrected towards the control after this extract treatment in this model. Furthermore, the seed extract corrected the levels of serum urea, uric acid, creatinine, cholesterol, triglyceride, and lipoproteins towards the control level in this experimental diabetic model. The results indicated the potentiality of the extract ofS. mahagoniseed for the correction of diabetes and its related complications like oxidative stress and hyperlipidemia. The extract may be a good candidate for developing a safety, tolerable, and promising neutraceutical treatment for the management of diabetes.

scholarly journals Antioxidant Effects of Eryngium carlinae in Diabetic Rats

2018 ◽  
Vol 6 (5) ◽  
Author(s):  
Diana García-Cerrillo ◽  
Ruth Noriega-Cisneros ◽  
Donovan Peña-Montes ◽  
Maribel Huerta-Cervantes ◽  
Mónica Silva-Ríos ◽  
...  
Keyword(s):  
Metabolic Diseases ◽  
Systemic Disease ◽  
Cardiac Injury ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Cardiovascular Risks ◽  
Thiobarbituric Acid Reactive Substances ◽  
Antioxidant Effects

Metabolic diseases have increased considerably such as diabetes mellitus (DM). Since diabetes is a systemic disease, it implies high cardiovascular risks. It has been widely established that cardiac injury is related to mitochondrial dysfunction through increment of reactive oxygen species (ROS). Synthetic antioxidants can have important side effects; therefore natural sources may represent a better option. Traditional Mexican medicine has been using Eryngium carlinae (EC) for medical treatment. Also our group showed that hexanic extract possesses in vitro antioxidant capacity. Experimental diabetes in Wistar rats was generated by streptozotocin (STZ) and hexanic extract of EC was supplied for 7 weeks (30 mg/kg). Cholesterol, triacylglycerides, glucose, and thiobarbituric acid reactive substances (TBARS) levels were determined in serum. Mitochondria from left ventricle were used in the quantification of TBARS, reduced glutathione, nitric oxide (NO) levels and activity of superoxide dismutase (SOD) enzyme was performed.  Biochemical parameters of glucose and triacylglycerides, as well as TBARS levels in serum show a significant reduction in diabetic group supplied with EC hexanic extract. Thus, we can conclude that the EC hexanic extract possesses antioxidant activity in vitro, and in vivo, by reducing glucose and triacylglycerides levels during hyperglycemia, which may eventually reduce the risk of developing diabetic cardiomyopathy.

scholarly journals Spirulina maxima and its effect on antioxidant activity in fructose induced oxidative stress with histopathological observations

2015 ◽  
Vol 62 (2) ◽  
pp. 13-19
Author(s):  
Urmila Jarouliya ◽  
Anish Zacharia ◽  
Raj K. Keservani ◽  
Godavarthi B.K.S Prasad
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Blood Glucose ◽  
Reduced Glutathione ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Therapeutic Effects ◽  
Thiobarbituric Acid Reactive Substances ◽  
Spirulina Maxima

Abstract Diabetes mellitus is a metabolic disorder characterised by hyperglycemia and oxidative stress. The aim of the present study is to explore the antioxidant effect of Spirulina maxima in rat model along with the histopathological observations. Diabetes was induced by feeding 10% fructose solution orally to Wistar rats (n = 6) for 30 days, analysed for plasma blood glucose and the markers of the oxidative stress [catalase (CAT), superoxide dismutase (SOD), reduced glutathione (GSH) and thiobarbituric acid reactive substances (TBARS)]. These biochemical studies were associated with histopathological examination of liver and kidney sections. The microalga Spirulina maxima being rich in proteins and other essential nutrients is widely used as a food supplement. S. maxima at a dose of 5 and 10% per kg and the metformin (500 mg/kg) as reference drug were given orally for 30 days to the diabetic rats. Diabetic rats showed significant (p < 0.001) elevations in plasma blood glucose, thiobarbituric acid-reactive substances and significant reduction in catalase, superoxide dismutase and reduced glutathione activity. Oral administration of 5 and 10% aqueous extract of S. maxima for 30 days restored not only of blood glucose levels but also markers of oxidative stress. Histopathological observations of tissues manifested that the S. maxima administration had the protective and therapeutic effects against fructose-induced abnormalities in diabetic rats. It is concluded that S. maxima is effective in reinstating the antioxidant activity in addition to its antidiabetic effect in type 2 diabetic rats.

scholarly journals Curcumin attenuates oxidative stress in liver in Type 1 diabetic rats

2017 ◽  
Vol 12 (1) ◽  
pp. 452-459 ◽  
Author(s):  
Zhenglu Xie ◽  
Xinqi Zeng ◽  
Xiaqing Li ◽  
Binbin Wu ◽  
Guozhi Shen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Polyol Pathway ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Curcumin Treatment ◽  
Kinase C ◽  
Diabetic Model ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Plasma Malondialdehyde

AbstractWe investigated the effect of curcumin on liver anti-oxidative stress in the type 1 diabetic rat model induced by streptozotocin (STZ). Experimental diabetic rats were induced by STZ intraperitoneally. All rats were fed for 21 days including three groups of control (NC), diabetic model (DC) and curcumin-treated (Cur, 1.5 g/kg by gavage). The results showed that curcumin-treatment significantly decreased the blood glucose and plasma malondialdehyde levels, but significantly increased the plasma superoxide dismutase, glutathione peroxidase and reduced glutathione levels. Curcumin treatment decreased the activity of aldose reductase, but increased the plasma glucose-6-phosphate dehydrogenase, glucose synthetase and glucose-polymerizing activities. Curcumin treatment significantly decreased the protein of protein kinase C (PKC) and poly ADP ribose polymerase (PARP) expression in the Cur group compared with the DC group. Moreover, the sorbitol dehydrogenase activity was significantly decreased and deterred glucose enters into the polyol pathway leading to an increased NADPH content in the Cur group compared with the DC group. Our data provides evidence that oxidative stress in diabetic rats may be attenuated by curcumin by inhibiting polyol pathway associated with down-regulated expression of PKC and PARP, as evidenced by both an increase the antioxidant enzymes levels and glycogen biosynthesis enzymes activities.

Vascular responses to agonists in rat mesenteric artery from diabetic rats

1987 ◽  
Vol 65 (7) ◽  
pp. 1484-1490 ◽  
Author(s):  
Devendra K. Agrawal ◽  
John H. McNeill
Keyword(s):  
Diabetic Rats ◽  
Isometric Tension ◽  
Mesenteric Arteries ◽  
Diabetic Rat ◽  
Prostaglandin E ◽  
Contractile Responses ◽  
Alpha Adrenoceptor ◽  
Diabetic Model ◽  
Rat Mesenteric Artery ◽  
Adrenoceptor Agonists

The effect of diabetes on vascular smooth muscle function was investigated in the muscular arteries from spontaneously and chemically induced diabetic rats. Isolated ring segments of superior mesenteric arteries from BB diabetic and streptozotocin (STZ)-diabetic rats (12 weeks after onset of diabetes) were used for isometric tension studies. Contractile responses to alpha-adrenoceptor agonists (norepinephrine, methoxamine, phenylephrine, B-HT 920, guanabenz, SKF 89748-A), serotonin, and K+ were significantly higher in STZ-diabetic rat arteries as compared with the controls. In spontaneously diabetic rat arteries only the contractile responses to the putatively selective alpha2-adrenoceptor agonists, K+ and prostaglandin E1, were significantly increased. pD2 values of the agonists in both groups of diabetic arteries were not significantly different from the respective controls. Nifedipine inhibited all contractile responses in a dose-dependent fashion. The responses to K+ and alpha2-adrenoceptor agonists were attenuated to a greater extent by nifedipine in both groups of diabetic blood vessels. The calcium channel activator, BAY K 8644, produced a twofold increase in force of contraction in streptozotocin-diabetic and spontaneously diabetic rat arteries as compared with the responses in their respective controls. These results suggest caution in extrapolating all the findings from the streptozotocin-induced diabetic model to the spontaneously diabetic model. However, increased activity of calcium channels in vascular muscle cells in both groups of diabetics may be responsible, at least in part, for the increased vascular contractility in diabetes mellitus.

scholarly journals Further Studies on Antioxidant Potential and Protection of Pancreaticβ-Cells byEmbelia ribesin Experimental Diabetes

2007 ◽  
Vol 2007 ◽  
pp. 1-6 ◽  
Author(s):  
Uma Bhandari ◽  
Neeti Jain ◽  
K. K. Pillai
Keyword(s):  
Antioxidant Defense ◽  
Ethanolic Extract ◽  
Thiobarbituric Acid ◽  
Pancreatic Tissue ◽  
Serum Lactate ◽  
Diabetic Rats ◽  
Oral Feeding ◽  
Serum Lactate Dehydrogenase ◽  
Thiobarbituric Acid Reactive Substances ◽  
Embelia Ribes

This study was designed to examine the antioxidant defense by ethanolic extract ofEmbelia ribeson streptozotocin-(40 mg/kg, intravenously, single-injection) induced diabetes in Wistar rats. Forty days of oral feeding the extract (100 mg/kg and 200 mg/kg) to diabetic rats resulted in significant(P<.01)decrease in blood glucose, blood glycosylated haemoglobin, serum lactate dehydrogenase, creatine kinase, and increase in blood glutathione levels as compared to pathogenic diabetic rats. Further, the extract also significantly(P<.01)decreased the pancreatic thiobarbituric acid-reactive substances (TBARS) levels and significantly(P<.01)increased the superoxide dismutase, catalase, and glutathione levels as compared to above levels in pancreatic tissue of pathogenic diabetic rats. The islets were shrunken in diabetic rats in comparison to normal rats. In the drug-treated diabetic rats, there was expansion of islets. The results of test drug were comparable to gliclazide (25 mg/kg, daily), a standard antihyperglycemic agent. The study concludes thatEmbelia ribesenhances the antioxidant defense against reactive oxygen species produced under hyperglycemic condition and this protectsβ-cells against loss, and exhibit antidiabetic property.

scholarly journals Antioxidant Status of Vigna mungo L. in Response to Sulfur Nutrition

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Neelam Chandra ◽  
Nalini Pandey
Keyword(s):  
Critical Concentration ◽  
Thiobarbituric Acid ◽  
Dry Weight ◽  
Vigna Mungo ◽  
Thiobarbituric Acid Reactive Substances ◽  
Young Leaves ◽  
Catalase Peroxidase ◽  
Sulfur Supply ◽  
Nonprotein Thiols ◽  
Sulfur Nutrition

Black gram (Vigna mungo L. var. DPU-88-31), an edible legume, was grown at 1, 2, 4, 6, and 8 meq S L−1 to study the effect of deficient and excess level of sulfur on oxidative metabolism. Plants supplied by 4 meq S L−1 showed optimum yield. Sulfur deficient plants (1 and 2 meq S L−1) showed reduction in growth and chlorosis of young leaves. Tissue sulfur and cysteine concentration was increased with increasing sulfur supply. The thresholds for critical concentration of sulfur deficiency and toxicity were 0.315% and 0.434% dry weight. Biomass and photoassimilatory pigments were decreased and carbohydrates (sugar and starch) were accumulated in leaves of sulfur deficient and excess plants. Accumulation of hydrogen peroxide and thiobarbituric acid reactive substances in sulfur deficient and excess plants caused oxidative damage in plants which was also evident by the increase in the activity of superoxide dismutase, catalase, peroxidase, ascorbate peroxidase, glutathione reductase, and concentration of ascorbate and nonprotein thiols.

Olive cake reduces glycaemia and lipemia and increases antioxidant enzymes in STZ-induced diabetes in rat erythrocytes and tissues

2019 ◽  
Vol 50 (2) ◽  
pp. 360-372
Author(s):  
Hayet Cherrad ◽  
Sherazede Bouderbala ◽  
Yahiaoui Zidan ◽  
Djamil Krouf
Keyword(s):  
Antioxidant Enzymes ◽  
Heart Muscle ◽  
Design Methodology ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Thiobarbituric Acid Reactive Substances ◽  
Olive Cake ◽  
Sod Activity ◽  
Content Type ◽  
Antioxidant Enzymes Activities

Purpose The purpose of this study is to determine the effect of olive cake (CO) on glycaemia and lipemia and lipid peroxidation and antioxidant enzymes activities in erythrocytes and tissues, in streptozotocin (STZ)-induced diabetic rats. Design/methodology/approach Diabetes was induced by a single intraperitoneal injection of STZ (55 mg/kg BW). In total, 12 diabetic D rats, weighing 260 ± 20 g, were divided into two groups fed a casein diet supplemented (D-OC) or not (D) with OC (7.5 per cent), for four weeks. Findings In D-OC compared with D, glycaemia, total cholesterol and triglycerides values (−40 per cent; p = 0.007, 27 per cent; p = 0.007 and −27 per cent; p = 0.0019). In erythrocyte, liver, kidney, heart, muscle and brain, thiobarbituric acid reactive substances contents were respectively, (−19 per cent; p = 0.03, −32 per cent; p = 0.002, −20 per cent; p = 0.04, −68 per cent; p = 0.003, −74 per cent; p = 0.0003 and −38 per cent; p = 0.04). In erythrocyte, SOD, GSH-Px and CAT activities were respectively, (+14 per cent; p = 0.01, +74 per cent; p = 0.012 and +34 per cent; p = 0.0009). In the liver, kidney, heart and muscle, SOD activity was respectively, (+31 per cent; p = 0.004, +12 per cent; p = 0.038, +43 per cent; p = 0.001 and +23 per cent; p = 0.18). GSH-Px activity was respectively, (+121 per cent; p = 0.0009, 89 per cent; p = 0.0006, + 95 per cent; p = 0.008, +71 per cent; p = 0.02 and +26 per cent; p = 0.01), in the liver, kidney, heart, muscle and brain. Catalase activity was (+21 per cent; p = 0.008) in the liver, (+88 per cent; p = 0.0002 in the kidney, +53 per cent; p = 0,002 in the heart and 83 per cent; p = 0.00004 in the muscle). Originality/value In diabetic rats, OC reduces hyperglycaemia induced by STZ and attenuates triglyceridemia and cholesterolemia. This residue is able to decrease the oxidative stress by increasing the antioxidant enzymes activity in erythrocytes and tissues. The high contents of phytoconstituents present in OC are considered to be responsible for this effect.

scholarly journals Orientin Attenuates Cisplatin-Induced Renal Toxicity by Reducing Oxidative Stress and Inflammation

2021 ◽  
Vol 41 (04) ◽  
pp. 574-578
Author(s):  
Muhammad Umar Ijaz
Keyword(s):  
Renal Damage ◽  
Renal Toxicity ◽  
Thiobarbituric Acid ◽  
Protective Role ◽  
Male Rats ◽  
Thiobarbituric Acid Reactive Substances ◽  
Catalase Peroxidase ◽  
Factor Α ◽  
Therapeutic Activities ◽  
Necrosis Factor

Cisplatin (CP), an effective chemotherapeutic drug, has been widely used to treat the several types of tumors. Orientin (ORI) is a flavonoid that shows versatile therapeutic activities. The current research was planned to observe the protective role ORI on CP induced renal injury in rats. Twenty-four male rats were divided into four groups equally and termed as control, CP (10 mg/kg), CP (10 mg/kg) + ORI (40 mg/kg) and ORI (40 mg/kg). After seven days trial, rats were dissected and different parameters were analyzed. Results indicated that the CP administration significantly reduced the activities of catalase, peroxidase, glutathione reductase and glutathione content whereas it increased the level of hydrogen peroxide and TBARS (thiobarbituric acid reactive substances). CP increased the creatinine and urea levels while decreased the creatinine clearance. Moreover, CP significantly increased the inflammatory markers, including nuclear factor kappa-B, tumor necrosis factor-α, Interleukin-6, Interleukin-1β levels, cyclooxygenase-2 activity and histopathological damages. However, co-administration of ORI displayed curative effects against CP-induced renal toxicity and recovered all parameters by bringing them to a normal level. These results revealed that the ORI is a potential bioflavonoid that can potentially counter the CP-induced renal damage.

scholarly journals DURATION DEPENDENT ANTIAPOPTOTIC EFFICACY OF CAMELLIA SINENSIS (GREEN TEA) ON STREPTOZOTOCIN INDUCED DIABETES LINKED TESTICULAR HYPOFUNCTION IN ALBINO RAT: GENOMIC AND FLOW CYTOMETRIC ASSESSMENT

2019 ◽  
pp. 85-93
Author(s):  
BARNALI DAS ◽  
BAISAKHI BISWAS ◽  
Debidas Ghosh
Keyword(s):  
Camellia Sinensis ◽  
Thiobarbituric Acid ◽  
Serum Testosterone ◽  
Ethyl Acetate Fraction ◽  
Diabetic Rat ◽  
Duration Of Treatment ◽  
Albino Rat ◽  
Significant Correction ◽  
Testicular Impairment ◽  
Threshold Duration

Objective: The study has been conducted to search out the threshold duration of treatment of ethyl acetate fraction of methanolic extract of leaves of Camellia sinensis (L.) Kuntze at the dose of 100 mg/kg body weight for the management of diabetes-induced testicular impairment in streptozotocin-induced diabetic rat in a duration dependent fashion. Methods: In this respect, the glycemic, androgenic, oxidative stress sensors, gene expression of testicular androgenic key enzymes along with apoptotic markers were evaluated in a duration dependent way (14, 28 and 56 d). Results: A significant correction was noted in the levels of glycated haemoglobin (HbA1C), testicular thiobarbituric acid reactive substances (TBARS), conjugated diene (CD), sperm viability, sperm mitochondrial status, serum testosterone, and genomic expression of testicular Δ5, 3β-hydroxysteroid dehydrogenase (HSD), 17β-HSD, Bax, Bcl-2 after treatment for different duration with the said fraction in diabetic groups in compare to respective vehicle-treated diabetic group without any toxicity induction in general. Thin layer chromatography (TLC) study of the fraction showed two spots with retention factors (Rf) of 0.78 and 0.51. Conclusion: The results showed that 28 d treatment was threshold duration of treatment for the correction of diabetes-induced testicular impairment.

Effects of damiana (Turnera diffusa; var. diffusa and var. aphrodisiaca) on diabetic rats

2019 ◽  
Vol 28 (6) ◽  
pp. 84-92
Author(s):  
Edgar Romualdo Esquivel-Gutiérrez ◽  
Lilia Alcaraz-Meléndez ◽  
Roberto Hernández-Herrera ◽  
Amada Torres ◽  
Carmen Rodríguez-Jaramillo
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Biochemical Parameters ◽  
Body Weight Loss ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Diabetic Rat ◽  
Glucose Levels ◽  
Turnera Diffusa ◽  
Ethanol Extracts

Turnera diffusa (damiana) grows wild in Baja California Sur (BCS), Mexico. It is traditionally used for its aphrodisiac effects and for diabetes treatment. Two varieties (var.) have been identified: diffusa and aphrodisiaca. To evaluate the effects of water-ethanol extracts from both varieties, measurements on blood glucose, body weight, biochemical parameters, and cell blood counts were performed in a diabetic rat model. Oxidative stress was also evaluated when var. diffusa was administered. No significant reductions in blood glucose levels, in biochemical parameters, or in cell blood counts with any variety of damiana were observed; however, it helped diabetic animals to keep their body weight. Damiana var. diffusa did not modify the mitochondrial nitric oxide levels and superoxide dismutase activity; however, thiobarbituric acid reactive species diminished. In conclusion, damiana can be utilized reliably in the management of diabetes because of its antioxidant potential; it promoted body weight loss in healthy animals and helped to preserve it in diabetes.

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