Mesenchymal endometrial stem/stromal cells for hard tissue engineering: a review of in vitro and in vivo evidence

2017 ◽  
Vol 12 (8) ◽  
pp. 983-995 ◽  
Author(s):  
Fahimeh S Tabatabaei ◽  
Jafar Ai
Keyword(s):  
Tissue Engineering ◽  
Stromal Cells ◽  
Hard Tissue ◽  
Hard Tissue Engineering

Bioactive glass containing 90% SiO 2 in hard tissue engineering: An in vitro and in vivo characterization study

2019 ◽  
Vol 13 (9) ◽  
pp. 1651-1663
Author(s):  
Luiz Felipe Cardoso Lehman ◽  
Mariana Saturnino Noronha ◽  
Ivana Márcia Alves Diniz ◽  
Rosangela Maria Ferreira Costa e Silva ◽  
Ângela Leão Andrade ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Hard Tissue ◽  
Characterization Study ◽  
In Vivo Characterization ◽  
Hard Tissue Engineering

Preparation and performance evaluation of electrospun poly(3-hydroxybutyrate) composite scaffolds as a potential hard tissue engineering application

2019 ◽  
Vol 34 (4-5) ◽  
pp. 386-400 ◽  
Author(s):  
Moein Zarei ◽  
Nader Tanideh ◽  
Shahrokh Zare ◽  
Fatemeh Sari Aslani ◽  
Omid Koohi-Hosseinabadi ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Carbon Nanotube ◽  
Engineering Application ◽  
Tissue Engineering Application ◽  
Hydroxyapatite Nanoparticles ◽  
Hard Tissue ◽  
Composite Scaffolds ◽  
Tissue Reactions ◽  
Hard Tissue Engineering

In the present study, poly(3-hydroxybutyrate)-based composite scaffolds were prepared with multi-walled carbon nanotubes and hydroxyapatite nanoparticles for hard tissue engineering applications by electrospinning. All the prepared scaffolds showed connective porous structure, which were suitable for cell proliferation and migration. The mechanical properties of the poly(3-hydroxybutyrate) scaffold were improved by 0.5% of carbon nanotube addition, whereas the addition of hydroxyapatite nanoparticles up to 10% had an insignificant effect in tensile strength. However, scanning electron microscopy and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay results suggested that the mesenchymal stem cells attachment and their metabolic activities on the surface of the poly(3-hydroxybutyrate) scaffolds with hydroxyapatite were enhanced compared to poly(3-hydroxybutyrate) scaffolds. In addition, after 6 weeks of in vivo biocompatibility results in a model of rat indicated better tissue reactions for the scaffolds that contained hydroxyapatite. Overall, poly(3-hydroxybutyrate) composite scaffolds with 10% hydroxyapatite and 0.5% carbon nanotube showed optimal performances for the potential scaffold for hard tissue engineering application.

scholarly journals Craniofacial Bone Tissue Engineering: Current Approaches and Potential Therapy

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 2993
Author(s):  
Arbi Aghali
Keyword(s):  
Tissue Engineering ◽  
Growth Factors ◽  
Bone Tissue ◽  
Stromal Cells ◽  
Critical Size ◽  
Tumor Resection ◽  
Bone Morphogenetic Protein 2 ◽  
Craniofacial Bone

Craniofacial bone defects can result from various disorders, including congenital malformations, tumor resection, infection, severe trauma, and accidents. Successfully regenerating cranial defects is an integral step to restore craniofacial function. However, challenges managing and controlling new bone tissue formation remain. Current advances in tissue engineering and regenerative medicine use innovative techniques to address these challenges. The use of biomaterials, stromal cells, and growth factors have demonstrated promising outcomes in vitro and in vivo. Natural and synthetic bone grafts combined with Mesenchymal Stromal Cells (MSCs) and growth factors have shown encouraging results in regenerating critical-size cranial defects. One of prevalent growth factors is Bone Morphogenetic Protein-2 (BMP-2). BMP-2 is defined as a gold standard growth factor that enhances new bone formation in vitro and in vivo. Recently, emerging evidence suggested that Megakaryocytes (MKs), induced by Thrombopoietin (TPO), show an increase in osteoblast proliferation in vitro and bone mass in vivo. Furthermore, a co-culture study shows mature MKs enhance MSC survival rate while maintaining their phenotype. Therefore, MKs can provide an insight as a potential therapy offering a safe and effective approach to regenerating critical-size cranial defects.

scholarly journals Chondrogenic Potential of Dental-Derived Mesenchymal Stromal Cells

Osteology ◽  
2021 ◽  
Vol 1 (3) ◽  
pp. 149-174
Author(s):  
Naveen Jeyaraman ◽  
Gollahalli Shivashankar Prajwal ◽  
Madhan Jeyaraman ◽  
Sathish Muthu ◽  
Manish Khanna
Keyword(s):  
Tissue Engineering ◽  
Mesenchymal Stromal Cells ◽  
Tissue Regeneration ◽  
Stromal Cells ◽  
Cartilage Tissue Engineering ◽  
Cartilage Regeneration ◽  
Cartilage Tissue ◽  
Dentin Matrix

The field of tissue engineering has revolutionized the world in organ and tissue regeneration. With the robust research among regenerative medicine experts and researchers, the plausibility of regenerating cartilage has come into the limelight. For cartilage tissue engineering, orthopedic surgeons and orthobiologists use the mesenchymal stromal cells (MSCs) of various origins along with the cytokines, growth factors, and scaffolds. The least utilized MSCs are of dental origin, which are the richest sources of stromal and progenitor cells. There is a paradigm shift towards the utilization of dental source MSCs in chondrogenesis and cartilage regeneration. Dental-derived MSCs possess similar phenotypes and genotypes like other sources of MSCs along with specific markers such as dentin matrix acidic phosphoprotein (DMP) -1, dentin sialophosphoprotein (DSPP), alkaline phosphatase (ALP), osteopontin (OPN), bone sialoprotein (BSP), and STRO-1. Concerning chondrogenicity, there is literature with marginal use of dental-derived MSCs. Various studies provide evidence for in-vitro and in-vivo chondrogenesis by dental-derived MSCs. With such evidence, clinical trials must be taken up to support or refute the evidence for regenerating cartilage tissues by dental-derived MSCs. This article highlights the significance of dental-derived MSCs for cartilage tissue regeneration.

scholarly journals Efficacy of Bacterial Nanocellulose in Hard Tissue Regeneration: A Review

Materials ◽  
2021 ◽  
Vol 14 (17) ◽  
pp. 4777
Author(s):  
Anuj Kumar ◽  
Sung-Soo Han
Keyword(s):  
Tissue Engineering ◽  
Tissue Regeneration ◽  
Cellular Response ◽  
Future Research ◽  
Hydroxyl Groups ◽  
Hard Tissue ◽  
Engineering Applications ◽  
Bacterial Nanocellulose

Bacterial nanocellulose (BNC, as exopolysaccharide) synthesized by some specific bacteria strains is a fascinating biopolymer composed of the three-dimensional pure cellulosic nanofibrous matrix without containing lignin, hemicellulose, pectin, and other impurities as in plant-based cellulose. Due to its excellent biocompatibility (in vitro and in vivo), high water-holding capacity, flexibility, high mechanical properties, and a large number of hydroxyl groups that are most similar characteristics of native tissues, BNC has shown great potential in tissue engineering applications. This review focuses on and discusses the efficacy of BNC- or BNC-based biomaterials for hard tissue regeneration. In this review, we provide brief information on the key aspects of synthesis and properties of BNC, including solubility, biodegradability, thermal stability, antimicrobial ability, toxicity, and cellular response. Further, modification approaches are discussed briefly to improve the properties of BNC or BNC-based structures. In addition, various biomaterials by using BNC (as sacrificial template or matrix) or BNC in conjugation with polymers and/or fillers are reviewed and discussed for dental and bone tissue engineering applications. Moreover, the conclusion with perspective for future research directions of using BNC for hard tissue regeneration is briefly discussed.

Cartilaginous Tissue Formation with an Elastic PLCL Scaffold and Human Adipose Tissue-Derived Stromal Cells

2007 ◽  
Vol 342-343 ◽  
pp. 385-388
Author(s):  
So Eun Lee ◽  
Young Mee Jung ◽  
Soo Hyun Kim ◽  
Sang Heon Kim ◽  
Jong Won Rhie ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Adipose Tissue ◽  
Stromal Cells ◽  
Cartilage Tissue Engineering ◽  
Human Adipose Tissue ◽  
Cartilage Tissue ◽  
Tissue Formation ◽  
Cartilaginous Tissue

In cartilage tissue engineering, as a cell source, adult stem cells are very attractive for clinical applications. Recent studies suggest that human adipose tissue-derived stromal cells (ASCs) have multilineage potential similar to bone marrow-derived stromal cells (BMSCs). ASCs are obtained from adipose tissue easily isolated by suction-assisted lipectomy in various body parts. Also, as one of major factors of cartilage tissue engineering, scaffolds have an important role in cartilage formation. Poly(L-lactide-co-ε-carprolactone) scaffolds have physiological activity, biodegradability, high cell affinity, and mechano-activity. The object of this study is cartilaginous tissue formation using highly elastic PLCL scaffolds and ASCs in vitro and in vivo. Poly(L-lactide-co-ε-carprolactone) copolymers were synthesized from lactide and ε-carprolactone in the presence of stannous octoate as catalyst. The scaffolds with 85% porosity and 300-500μm pore size were fabricated by gel-pressing method. ASCs were seeded on scaffolds and cultured for 21days in vitro. Cell/polymer constructs were characterized by reverse transcriptase-polymerase chain reaction for confirming differentiation to chondrocytes onto PLCL scaffolds. Also, for examining cartilaginous tissue formation in vivo, ASCs seeded scaffolds which were induced chondrogenesis for 2 weeks were implanted in nude mice subcutaneously for up to 8weeks. Histological studies showed that implants partially developed cartilaginous tissue within lacunae. And there was an accumulation of sulfated glycoaminoglycans. Immunohistochemical analysis revealed that implants were positively stained for specific extracellular matrix. These results indicate that ASCs and PLCL scaffols could be used to cartilage tissue engineering.

scholarly journals A Critical Review on Polymeric Biomaterials for Biomedical Applications

Polymers ◽  
2021 ◽  
Vol 13 (17) ◽  
pp. 3015
Author(s):  
Cheirmadurai Kalirajan ◽  
Amey Dukle ◽  
Arputharaj Joseph Nathanael ◽  
Tae-Hwan Oh ◽  
Geetha Manivasagam
Keyword(s):  
Tissue Engineering ◽  
Biomedical Applications ◽  
Self Healing ◽  
Polymeric Biomaterials ◽  
Future Direction ◽  
Hard Tissue Engineering ◽  
In Vivo Effects ◽  
New Strategies

Natural and synthetic polymers have been explored for many years in the field of tissue engineering and regeneration. Researchers have developed many new strategies to design successful advanced polymeric biomaterials. In this review, we summarized the recent notable advancements in the preparation of smart polymeric biomaterials with self-healing and shape memory properties. We also discussed novel approaches used to develop different forms of polymeric biomaterials such as films, hydrogels and 3D printable biomaterials. In each part, the applications of the biomaterials in soft and hard tissue engineering with their in vitro and in vivo effects are underlined. The future direction of the polymeric biomaterials that could pave a path towards successful clinical implications is also underlined in this review.

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