Research on in vitro and in vivo biocompatibility of the low-friction Ti+C/amorphous carbon gradient multilayer films for hard tissue engineering

In the present study, poly(3-hydroxybutyrate)-based composite scaffolds were prepared with multi-walled carbon nanotubes and hydroxyapatite nanoparticles for hard tissue engineering applications by electrospinning. All the prepared scaffolds showed connective porous structure, which were suitable for cell proliferation and migration. The mechanical properties of the poly(3-hydroxybutyrate) scaffold were improved by 0.5% of carbon nanotube addition, whereas the addition of hydroxyapatite nanoparticles up to 10% had an insignificant effect in tensile strength. However, scanning electron microscopy and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay results suggested that the mesenchymal stem cells attachment and their metabolic activities on the surface of the poly(3-hydroxybutyrate) scaffolds with hydroxyapatite were enhanced compared to poly(3-hydroxybutyrate) scaffolds. In addition, after 6 weeks of in vivo biocompatibility results in a model of rat indicated better tissue reactions for the scaffolds that contained hydroxyapatite. Overall, poly(3-hydroxybutyrate) composite scaffolds with 10% hydroxyapatite and 0.5% carbon nanotube showed optimal performances for the potential scaffold for hard tissue engineering application.

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Efficacy of Bacterial Nanocellulose in Hard Tissue Regeneration: A Review

Materials ◽

10.3390/ma14174777 ◽

2021 ◽

Vol 14 (17) ◽

pp. 4777

Author(s):

Anuj Kumar ◽

Sung-Soo Han

Keyword(s):

Tissue Engineering ◽

Tissue Regeneration ◽

Cellular Response ◽

Future Research ◽

Hydroxyl Groups ◽

Hard Tissue ◽

Engineering Applications ◽

Bacterial Nanocellulose

Bacterial nanocellulose (BNC, as exopolysaccharide) synthesized by some specific bacteria strains is a fascinating biopolymer composed of the three-dimensional pure cellulosic nanofibrous matrix without containing lignin, hemicellulose, pectin, and other impurities as in plant-based cellulose. Due to its excellent biocompatibility (in vitro and in vivo), high water-holding capacity, flexibility, high mechanical properties, and a large number of hydroxyl groups that are most similar characteristics of native tissues, BNC has shown great potential in tissue engineering applications. This review focuses on and discusses the efficacy of BNC- or BNC-based biomaterials for hard tissue regeneration. In this review, we provide brief information on the key aspects of synthesis and properties of BNC, including solubility, biodegradability, thermal stability, antimicrobial ability, toxicity, and cellular response. Further, modification approaches are discussed briefly to improve the properties of BNC or BNC-based structures. In addition, various biomaterials by using BNC (as sacrificial template or matrix) or BNC in conjugation with polymers and/or fillers are reviewed and discussed for dental and bone tissue engineering applications. Moreover, the conclusion with perspective for future research directions of using BNC for hard tissue regeneration is briefly discussed.

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A Critical Review on Polymeric Biomaterials for Biomedical Applications

Polymers ◽

10.3390/polym13173015 ◽

2021 ◽

Vol 13 (17) ◽

pp. 3015

Author(s):

Cheirmadurai Kalirajan ◽

Amey Dukle ◽

Arputharaj Joseph Nathanael ◽

Tae-Hwan Oh ◽

Geetha Manivasagam

Keyword(s):

Tissue Engineering ◽

Biomedical Applications ◽

Self Healing ◽

Polymeric Biomaterials ◽

Future Direction ◽

Hard Tissue Engineering ◽

In Vivo Effects ◽

New Strategies

Natural and synthetic polymers have been explored for many years in the field of tissue engineering and regeneration. Researchers have developed many new strategies to design successful advanced polymeric biomaterials. In this review, we summarized the recent notable advancements in the preparation of smart polymeric biomaterials with self-healing and shape memory properties. We also discussed novel approaches used to develop different forms of polymeric biomaterials such as films, hydrogels and 3D printable biomaterials. In each part, the applications of the biomaterials in soft and hard tissue engineering with their in vitro and in vivo effects are underlined. The future direction of the polymeric biomaterials that could pave a path towards successful clinical implications is also underlined in this review.

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PDLLA/Bioglass® composites for soft-tissue and hard-tissue engineering: an in vitro cell biology assessment

Biomaterials ◽

10.1016/j.biomaterials.2003.09.081 ◽

2004 ◽

Vol 25 (15) ◽

pp. 3013-3021 ◽

Cited By ~ 212

Author(s):

Sophie Verrier ◽

Jonny J. Blaker ◽

Veronique Maquet ◽

Larry L. Hench ◽

Aldo R. Boccaccini

Keyword(s):

Tissue Engineering ◽

Soft Tissue ◽

Cell Biology ◽

Hard Tissue ◽

Hard Tissue Engineering

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Decellularized bone extracellular matrix in skeletal tissue engineering

Biochemical Society Transactions ◽

10.1042/bst20190079 ◽

2020 ◽

Vol 48 (3) ◽

pp. 755-764

Author(s):

Benjamin B. Rothrauff ◽

Rocky S. Tuan

Keyword(s):

Tissue Engineering ◽

Bone Regeneration ◽

Tissue Regeneration ◽

Animal Studies ◽

Tumor Resection ◽

Regenerative Capacity ◽

Skeletal Tissue ◽

Large Bone

Bone possesses an intrinsic regenerative capacity, which can be compromised by aging, disease, trauma, and iatrogenesis (e.g. tumor resection, pharmacological). At present, autografts and allografts are the principal biological treatments available to replace large bone segments, but both entail several limitations that reduce wider use and consistent success. The use of decellularized extracellular matrices (ECM), often derived from xenogeneic sources, has been shown to favorably influence the immune response to injury and promote site-appropriate tissue regeneration. Decellularized bone ECM (dbECM), utilized in several forms — whole organ, particles, hydrogels — has shown promise in both in vitro and in vivo animal studies to promote osteogenic differentiation of stem/progenitor cells and enhance bone regeneration. However, dbECM has yet to be investigated in clinical studies, which are needed to determine the relative efficacy of this emerging biomaterial as compared with established treatments. This mini-review highlights the recent exploration of dbECM as a biomaterial for skeletal tissue engineering and considers modifications on its future use to more consistently promote bone regeneration.

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In vitro- / in vivo- Untersuchungen zur Biokompatibilität und Bioresorption amorpher Kieselgelfaser für das Tissue engineering humaner Knorpelgewebe

Laryngo-Rhino-Otologie ◽

10.1055/s-2004-823584 ◽

2004 ◽

Vol 83 (02) ◽

Author(s):

A Haisch ◽

A Evers ◽

K Jöhrens-Leder ◽

S Jovanovic ◽

B Sedlmaier ◽

...

Keyword(s):

Tissue Engineering

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Surface Modification by Nanobiomaterials for Vascular Tissue Engineering Applications

Current Medicinal Chemistry ◽

10.2174/0929867325666180914104633 ◽

2020 ◽

Vol 27 (10) ◽

pp. 1634-1646 ◽

Cited By ~ 1

Author(s):

Huey-Shan Hung ◽

Shan-hui Hsu

Keyword(s):

Tissue Engineering ◽

Vascular Tissue ◽

Thrombus Formation ◽

Vascular Grafts ◽

Vascular Tissue Engineering ◽

Great Success ◽

Natural Polymers ◽

Vascular Biomaterials

Treatment of cardiovascular disease has achieved great success using artificial implants, particularly synthetic-polymer made grafts. However, thrombus formation and restenosis are the current clinical problems need to be conquered. New biomaterials, modifying the surface of synthetic vascular grafts, have been created to improve long-term patency for the better hemocompatibility. The vascular biomaterials can be fabricated from synthetic or natural polymers for vascular tissue engineering. Stem cells can be seeded by different techniques into tissue-engineered vascular grafts in vitro and implanted in vivo to repair the vascular tissues. To overcome the thrombogenesis and promote the endothelialization effect, vascular biomaterials employing nanotopography are more bio-mimic to the native tissue made and have been engineered by various approaches such as prepared as a simple surface coating on the vascular biomaterials. It has now become an important and interesting field to find novel approaches to better endothelization of vascular biomaterials. In this article, we focus to review the techniques with better potential improving endothelization and summarize for vascular biomaterial application. This review article will enable the development of biomaterials with a high degree of originality, innovative research on novel techniques for surface fabrication for vascular biomaterials application.

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Collagen-Based Electrospun Materials for Tissue Engineering: A Systematic Review

Bioengineering ◽

10.3390/bioengineering8030039 ◽

2021 ◽

Vol 8 (3) ◽

pp. 39

Author(s):

Britani N. Blackstone ◽

Summer C. Gallentine ◽

Heather M. Powell

Keyword(s):

Systematic Review ◽

Tissue Engineering ◽

Collagen Type I ◽

The Body ◽

Pool Data ◽

Type I ◽

High Concentrations ◽

Increased Strength

Collagen is a key component of the extracellular matrix (ECM) in organs and tissues throughout the body and is used for many tissue engineering applications. Electrospinning of collagen can produce scaffolds in a wide variety of shapes, fiber diameters and porosities to match that of the native ECM. This systematic review aims to pool data from available manuscripts on electrospun collagen and tissue engineering to provide insight into the connection between source material, solvent, crosslinking method and functional outcomes. D-banding was most often observed in electrospun collagen formed using collagen type I isolated from calfskin, often isolated within the laboratory, with short solution solubilization times. All physical and chemical methods of crosslinking utilized imparted resistance to degradation and increased strength. Cytotoxicity was observed at high concentrations of crosslinking agents and when abbreviated rinsing protocols were utilized. Collagen and collagen-based scaffolds were capable of forming engineered tissues in vitro and in vivo with high similarity to the native structures.

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