The repair of alveolar cleft bone defect in rabbits by active bone particles containing modified rhBMP-2

2021 ◽  
Author(s):  
Dan Zhang ◽  
Xue-Cheng Sun ◽  
Hu Wang ◽  
Jian-Hui Li ◽  
Li-Qiang Yin ◽  
...  
Keyword(s):  
Bone Repair ◽  
Biochemical Analysis ◽  
Bone Collagen ◽  
Alveolar Cleft ◽  
Blood Biochemical ◽  
Old Japanese ◽  
Good Biocompatibility ◽  
Operation Results ◽  
Bone Particles ◽  
Better Than

Objective: A model of alveolar cleft phenotype was established in rabbits to evaluate the effect of active bone particles containing modified rhBMP-2 on the repair of the alveolar cleft. Methods: 2-month-old Japanese white rabbits were selected and randomly divided into four groups: normal, control, material and BMP groups. Blood biochemical analysis, skull tomography (microfocus computerized tomography), and histological and immunohistochemical staining analysis of paraffin sections were performed 3 months and 6 months after operation. Results: Both types of collagen particles showed good biocompatibility and promoted bone regeneration. The effect of active bone particles on bone repair and regeneration was better than that of bone collagen particles. Conclusions: Active bone particles containing modified rhecombinant human MP-2 can be used for incisors regeneration.

scholarly journals Repair of alveolar cleft bone defects by bone collagen particles combined with hUC-MSCs in rabbit

2020 ◽  
Author(s):  
Xue-Cheng Sun ◽  
Hu Wang ◽  
Jian-Hui Li ◽  
Dan Zhang ◽  
Xu Ma ◽  
...  
Keyword(s):  
Bone Repair ◽  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Bone Defects ◽  
Normal Group ◽  
Bone Collagen ◽  
Control Group ◽  
Human Umbilical Cord ◽  
Physical And Mental Health ◽  
Alveolar Cleft

Abstract Background: Alveolar cleft is a kind of cleft lip and palate, which seriously affects the physical and mental health of patients. In this study, a similar model of human alveolar cleft phenotype was established in rabbits to evaluate the effect of bone collagen particles combined with human umbilical cord mesenchymal stem cells (hUC-MSCs) on the repair of alveolar cleft bone defects. Methods: In this study, 24 adult Japanese white rabbits (JWRs) were selected and randomly divided into 4 groups. Including normal group, control group, materials group and MSCs group. The model of alveolar clefts was established by removing the incisors on the left side of the upper jaw. The normal group did nothing. In the control group, the incisors were removed and sutured directly. In the material group, the incisor were removed, then filled with bone collagen particles, and finally sutured. In the MSCs group, the incisors were first removed, then filled with bone collagen particles incubated by hUC-MSCs, and then stitched. Blood biochemical analysis was performed 3 months after the operation. Skull tissues were collected for gross observation, and micro-focus computerized tomography (micro-CT) analysis. Paraffin sections were prepared for histological and immunohistochemical staining. Results: The bone collagen particles and hUC-MSCs are not biotoxic and can promote alvenlus regeneration. Bone collagen particles combined with hUC-MSCs were much better than those used alone in inducing bone repair and regeneration. Conclusions: HUC-MSCs can be used as a bone generation inducer combined with bone collagen materials for bone regeneration and repair.

scholarly journals Repair of alveolar cleft bone defects by bone collagen particles combined with human umbilical cord mesenchymal stem cells in rabbit

2020 ◽  
Author(s):  
Xue-Cheng Sun ◽  
Hu Wang ◽  
Jian-Hui Li ◽  
Dan Zhang ◽  
Xu Ma ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Bone Repair ◽  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Bone Defects ◽  
Bone Collagen ◽  
Human Umbilical Cord ◽  
Alveolar Cleft

Abstract Background: Alveolar cleft is a kind of cleft lip and palate, which seriously affects the physical and mental health of patients. In this study ， the model of the alveolar cleft phenotype was established in rabbits to evaluate the effect of bone collagen particles combined with human umbilical cord mesenchymal stem cells (HUC-MSCs) on the repair of alveolar cleft bone defects. Methods : The model of alveolar clefts in rabbits was established by removing the incisors on the left side of the upper jaw. Bone collagen particles combined with hUC-MSCs were implanted in the defect area. Blood biochemical analysis was performed after 3 months. Skull tissues were harvested for gross observation, and micro-focus computerized tomography (micro-CT) analysis. Tissues were harvested for histological and immunohistochemical staining. The experiments were repeated 6 months after surgery. Results: The bone collagen particles and HUC-MSCs have good biological safety. In addition, both can promote the regeneration of incisor. Bone collagen particles combined with hUC-MSCs were much better than those used alone in inducing bone repair and regeneration. Conclusions: The method of HUC-MSCs combined with bone collagen particle material to fill a bone defect site is simple, rapid and suitable for the treatment of alveolar cleft bone defects.

Repair of lumbar vertebral bone defects by bone particles combined with hUC-MSCs in weaned rabbit

2019 ◽  
Vol 14 (10) ◽  
pp. 915-923
Author(s):  
Yi Cui ◽  
Bai Xu ◽  
Yanyun Yin ◽  
Bing Chen ◽  
Yannan Zhao ◽  
...  
Keyword(s):  
Bone Repair ◽  
Spinal Dysraphism ◽  
Bone Collagen ◽  
Human Umbilical Cord ◽  
Bovine Bone ◽  
Defect Area ◽  
New Strategy ◽  
Arch Reconstruction ◽  
Area X ◽  
Bone Particles

Aim: The major symptom of many closed spinal dysraphism patients is that the laminas or arches of vertebra are not fused well. To date, the bone repair of spina bifida for young children is a significant challenge in clinical practice. Materials & methods: Bovine bone collagen particle (BBCP) scaffolds combined with human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) were implanted in the defect area. X-ray analysis was performed after 3 months. Tissues were harvested for gross observation, and histological and immunohistochemical staining. Results: The BBCP supported hUC-MSCs adhesion and growth. Implanted BBCP combined with hUC-MSCs also promoted bone regeneration in the vertebral lamina and arch defect area. Conclusion: This method represents a new strategy for vertebral lamina and arch reconstruction in children.

scholarly journals Microsphere-Based Microfluidic Device for Plasma Separation and Potential Biochemistry Analysis Applications

Micromachines ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 487
Author(s):  
Hongyan Xu ◽  
Zhangying Wu ◽  
Jinan Deng ◽  
Jun Qiu ◽  
Ning Hu ◽  
...  
Keyword(s):  
Blood Sample ◽  
Microfluidic Device ◽  
Biochemical Analysis ◽  
Cost Effective ◽  
Clinical Diagnostics ◽  
Correction Factors ◽  
Separation Device ◽  
Blood Samples ◽  
Plasma Separation ◽  
Blood Biochemical

The development of a simple, portable, and cost-effective plasma separation platform for blood biochemical analysis is of great interest in clinical diagnostics. We represent a plasma separation microfluidic device using microspheres with different sizes as the separation barrier. This plasma separation device, with 18 capillary microchannels, can extract about 3 μL of plasma from a 50 μL blood sample in about 55 min. The effects of evaporation and the microsphere barrier on the plasma biochemical analysis results were studied. Correction factors were applied to compensate for these two effects. The feasibility of the device in plasma biochemical analysis was validated with clinical blood samples.

Fabrication and biological evaluation of porous β-TCP bioceramics produced using digital light processing

2021 ◽  
pp. 095441192110411
Author(s):  
SongFeng Xu ◽  
Hang Zhang ◽  
Xiang Li ◽  
XinXin Zhang ◽  
HuanMei Liu ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Bone Repair ◽  
Femoral Condyle ◽  
Biological Evaluation ◽  
Mechanical Tests ◽  
Digital Light Processing ◽  
Beta Tricalcium Phosphate ◽  
Ectopic Bone ◽  
Good Biocompatibility ◽  
Mouse Osteoblast

Beta-tricalcium phosphate ( β-TCP) refers to one ideal bone repair substance with good biocompatibility and osteogenicity. A digital light processing (DLP)-system used in this study creates bioceramic green part by stacking up layers of photocurable tricalcium phosphate-filled slurry with various β-TCP weight fractions. Results show that the sintering shrinkage is anisotropic and the shrinkage vertically reaches over that horizontally. The obtained porous β-TCP parts have both macroporous outer structure and microporous inner structure, the macropore size is 400–600 μm and the micropore size is 500–1500 nm. The mechanical tests show that the porous β-TCP bioceramic’s compressive strength reaches 16.53 MPa. The cell culture confirmed that the porous β-TCP bioceramic is capable of achieving the effective attaching, growing, and proliferating pertained to mouse osteoblast cells. This study identified considerable blood vessels and significant ectopic bone forming obviously based on the histologically-related assessment when implanting to rabbit femoral condyle deficiency for 3 months. Thus, under high bioactive property and osteoinductivity, and large precision and mechanical strength that can be adjusted, the DLP printed porous β-TCP ceramics is capable of being promising for special uses of bones repairing.

scholarly journals Poly (Glycerol Sebacate)-Based Bio-Artificial Multiporous Matrix for Bone Regeneration

2020 ◽  
Vol 8 ◽  
Author(s):  
Bo Liang ◽  
Qiang Shi ◽  
Jia Xu ◽  
Yi-Min Chai ◽  
Jian-Guang Xu
Keyword(s):  
Bone Tissue ◽  
Bone Repair ◽  
Bone Tissue Regeneration ◽  
Osteogenic Potential ◽  
Allogeneic Bone ◽  
Material Synthesis ◽  
Native Bone ◽  
High Elasticity ◽  
Good Biocompatibility ◽  
Endothelial Growth Factor

In recent years, bone repair biomaterials that combine cells and bioactive factors are superior to autologous and allogeneic bone implants. However, neither natural nor synthetic biomaterials can possess all desired qualities such as strength, porosity, and biological activity. In this study, we used poly (glycerol sebacate) (PGS), a synthetic material with great osteogenic potential that has attracted more attention in the field of tissue (such as bone tissue) regeneration owing to its good biocompatibility and high elasticity. It also has the advantage of being regulated by material synthesis to match the bone tissue's strength and can be easily modified to become functional. However, pure PGS lacks functional groups and hydrophilicity. Therefore, we used PGS as the substrate to graft the adhesive ligands RGD and vascular endothelial growth factor mimetic peptide. The bone repair scaffold can be prepared through photo crosslinking, as it not only improves hydrophobicity but also promotes vascularization and accelerates osteogenesis. Simultaneously, we improved the preparation method of hydrogels after freeze-drying and crosslinking to form a sponge-like structure and to easily regenerate blood vessels. In summary, a bone repair scaffold was prepared to meet the structural and biological requirements. It proved to serve as a potential bone-mimicking scaffold by enhancing tissue regenerative processes such as cell infiltration and vascularization and subsequent replacement by the native bone tissue.

scholarly journals Effect of functional beetroot pomace biscuit on phenylhydrazine induced anemia in albino rats: Hematological and blood biochemical analysis

2021 ◽  
Vol 78 ◽  
pp. 104385
Author(s):  
Eman M. Abdo ◽  
Omayma El-Sayed Shaltout ◽  
Sobhy El-Sohaimy ◽  
Ahmed E.M. Abdalla ◽  
Ahmed M. Zeitoun
Keyword(s):  
Biochemical Analysis ◽  
Albino Rats ◽  
Blood Biochemical

scholarly journals Evaluation of Genotoxic And Biochemical Stress Response To Mercuric Chloride In Erythrocytes of Freshwater Fish Channa Punctatus

2021 ◽  
Author(s):  
Harmanpreet Kaur Gill ◽  
Kapil Paul ◽  
Anish Dua
Keyword(s):  
Stress Response ◽  
Comet Assay ◽  
Mercuric Chloride ◽  
Biochemical Analysis ◽  
Micronucleus Assay ◽  
Blood Biochemistry ◽  
Channa Punctatus ◽  
Genotoxic Effects ◽  
Toxicity Assay ◽  
Blood Biochemical

Abstract Experiments were conducted to evaluate health of fish, Channa punctatus inhabiting mercuric chloride. Acute toxicity bioassays were performed to calculate 96h LC50 value and it was found to be 1.38mg/L. Chronic toxicity assay to investigate genotoxic effects on the erythrocytes of fish by comet assay and micronucleus assay along with alterations in blood biochemistry were evaluated. Results showed concentration and duration dependent significant DNA damage as observed by comet assay and micronucleus assay. The frequency of nuclear aberrations along with appearance of micronuclei were observed after 30 and 60 days of exposure. The blood biochemistry was studied by recording changes in levels of various biochemical parameters in blood serum and results showed the significant (p < 0.05) variations among levels of biochemical constituents such as Glucose, Lipids, Proteins, Bilirubin, Urea, Creatinine, Cholesterol, ALP, Albumin, SGOT, SGPT and Total Glycerides. The resultsindicated the stress response of specimen towards toxicant. The present study highly recommends the use of genotoxicity and blood biochemical analysis as the useful biomarker to assess toxicity in the aquatic water and hence to safeguards the surrounding ecosystem.

scholarly journals Monitoring of Physiological Changes of Uric Acid Concentration in the Blood of Snakes

2017 ◽  
Vol 61 (2) ◽  
pp. 56-60
Author(s):  
A. Lam ◽  
M. Halán
Keyword(s):  
Uric Acid ◽  
Renal Disease ◽  
Biochemical Analysis ◽  
Biochemical Parameter ◽  
Uric Acid Concentration ◽  
Blood Biochemical ◽  
Repeated Sampling ◽  
Induced Changes ◽  
The Relationship ◽  
Basal Concentration

AbstractThe evaluation of uric acid concentrations in the blood of snakes is a crucial tool in the diagnosis of gout and renal disease; both prevalent diseases in captive reptiles. However, without an understanding of the physiological fluctuations in uric acid levels and the absence of distinction that makes pathological changes, biochemical parameters are devalued. This study focuses on investigating the relationship between feeding rate and plasma-uric acid concentrations of snakes. The aim of this investigation is to facilitate a better understanding of the feed-induced changes that occur, and to render the analysis of this biochemical parameter as a more potent diagnostic tool. A total of 10 snakes were used in the study and the basal concentration of uric acid was established prior to feeding via blood biochemical analysis. The snakes were then fed rats and successive postprandial blood samples were taken for the monitoring of uric acid levels. The results demonstrated that feeding led to substantial elevations in the uric acid values, whereby postprandial concentrations were significantly elevated for up to 5 days after feeding. The postprandial elevations in uric acid documented in these snakes were of similar levels reported in snakes afflicted with gout or renal disease. The results demonstrated the significant changes that occur to uric acid levels after feeding, and highlights the resemblance between postprandial increases in uric acid and concentrations reported in snakes suffering from renal disease or gout. To avoid a misdiagnosis and to distinguish transient postprandial hyperuricemia from pathological elevations, collecting sufficient anamnestic data on time since last feeding in performing repeated sampling after one week period of fasting is suggested.

scholarly journals Graphene oxide-modified silk fibroin/nanohydroxyapatite scaffold loaded with urine-derived stem cells for immunomodulation and bone regeneration

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jiachen Sun ◽  
Lang Li ◽  
Fei Xing ◽  
Yun Yang ◽  
Min Gong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graphene Oxide ◽  
Bone Regeneration ◽  
Silk Fibroin ◽  
Bone Repair ◽  
Minimum Cost ◽  
Histological Evaluation ◽  
Micro Computed Tomography ◽  
Cranial Defect ◽  
Good Biocompatibility

Abstract Background The invasive and complicated procedures involving the use of traditional stem cells limit their application in bone tissue engineering. Cell-free, tissue-engineered bones often have complex scaffold structures and are usually engineered using several growth factors (GFs), thus leading to costly and difficult preparations. Urine-derived stem cells (USCs), a type of autologous stem cell isolated noninvasively and with minimum cost, are expected to solve the typical problems of using traditional stem cells to engineer bones. In this study, a graphene oxide (GO)-modified silk fibroin (SF)/nanohydroxyapatite (nHA) scaffold loaded with USCs was developed for immunomodulation and bone regeneration. Methods The SF/nHA scaffolds were prepared via lyophilization and cross-linked with GO using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxy succinimide (NHS). Scaffolds containing various concentrations of GO were characterized using scanning electron microscopy (SEM), the elastic modulus test, Fourier transform infrared spectroscopy (FTIR), and X-ray photoelectron spectrometer (XPS). Examinations of cell adhesion, proliferation, viability, morphology, alkaline phosphatase activity, and osteogenesis-related gene expression were performed to compare the osteogenesis-related biological behaviors of USCs cultured on the scaffolds. The effect of USC-laden scaffolds on the differentiation of macrophages was tested using ELISA, qRT-PCR, and immunofluorescence staining. Subcutaneous implantations in rats were performed to evaluate the inflammatory response of the USC-laden scaffolds after implantation. The scaffolds loaded with USCs were implanted into a cranial defect model in rats to repair bone defects. Micro-computed tomography (μCT) analyses and histological evaluation were performed to evaluate the bone repair effects. Results GO modification enhanced the mechanical properties of the scaffolds. Scaffolds containing less than 0.5% GO had good biocompatibility and promoted USC proliferation and osteogenesis. The scaffolds loaded with USCs induced the M2-type differentiation and inhibited the M1-type differentiation of macrophages. The USC-laden scaffolds containing 0.1% GO exhibited the best capacity for promoting the M2-type differentiation of macrophages and accelerating bone regeneration and almost bridged the site of the rat cranial defects at 12 weeks after surgery. Conclusions This composite system has the capacity for immunomodulation and the promotion of bone regeneration and shows promising potential for clinical applications of USC-based, tissue-engineered bones.

Sign in / Sign up

Export Citation Format

Share Document