Oral Administration of Lactoferrin Enhances the Productions of IFN-γ and IL-10 in Spleen Cells Cultured with Concanavalin A or Lipopolysaccharide

ABSTRACT Trypanosoma cruzi (Y strain)-infected interleukin-4−/− (IL-4−/−) mice of strains 129/J, BALB/c, and C57BL/6 showed no significant difference in parasitemia levels or end point mortality rates compared to wild-type (WT) mice. Higher production of gamma interferon (IFN-γ) by parasite antigen (Ag)-stimulated splenocytes was observed only for C57BL/6 IL-4−/− mice. Treatment of 129/J WT mice with recombinant IL-4 (rIL-4), rIL-10, anti-IL-4, and/or anti-IL-10 monoclonal antibodies (MAbs) did not modify parasitism. However, WT mice treated with rIL-4 and rIL-10 had markedly increased parasitism and suppressed IFN-γ synthesis by spleen cells stimulated with parasite Ag, concanavalin A, or anti-CD3. Addition of anti-IL-4 MAbs to splenocyte cultures from infected WT 129/J, BALB/c, or C57BL/6 mice failed to modify IFN-γ synthesis levels; in contrast, IL-10 neutralization increased IFN-γ production and addition of rIL-4 and/or rIL-10 diminished IFN-γ synthesis. We conclude that endogenous IL-4 is not a major determinant of susceptibility to Y strain T. cruziinfection but that IL-4 can, in association with IL-10, modulate IFN-γ production and resistance.

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Gomisin M2 Ameliorates Atopic Dermatitis-like Skin Lesions via Inhibition of STAT1 and NF-κB Activation in 2,4-Dinitrochlorobenzene/Dermatophagoides farinae Extract-Induced BALB/c Mice

Molecules ◽

10.3390/molecules26154409 ◽

2021 ◽

Vol 26 (15) ◽

pp. 4409

Author(s):

Jinjoo Kang ◽

Soyoung Lee ◽

Namkyung Kim ◽

Hima Dhakal ◽

Taeg-Kyu Kwon ◽

...

Keyword(s):

Atopic Dermatitis ◽

Oral Administration ◽

Skin Diseases ◽

Nuclear Translocation ◽

Skin Lesions ◽

Biologically Active ◽

Therapeutic Effects ◽

Dermatophagoides Farinae ◽

Tnf Α ◽

Ifn Γ

The extracts of Schisandra chinensis (Turcz.) Baill. (Schisandraceae) have various therapeutic effects, including inflammation and allergy. In this study, gomisin M2 (GM2) was isolated from S. chinensis and its beneficial effects were assessed against atopic dermatitis (AD). We evaluated the therapeutic effects of GM2 on 2,4-dinitrochlorobenzene (DNCB) and Dermatophagoides farinae extract (DFE)-induced AD-like skin lesions with BALB/c mice ears and within the tumor necrosis factor (TNF)-α and interferon (IFN)-γ-stimulated keratinocytes. The oral administration of GM2 resulted in reduced epidermal and dermal thickness, infiltration of tissue eosinophils, mast cells, and helper T cells in AD-like lesions. GM2 suppressed the expression of IL-1β, IL-4, IL-5, IL-6, IL-12a, and TSLP in ear tissue and the expression of IFN-γ, IL-4, and IL-17A in auricular lymph nodes. GM2 also inhibited STAT1 and NF-κB phosphorylation in DNCB/DFE-induced AD-like lesions. The oral administration of GM2 reduced levels of IgE (DFE-specific and total) and IgG2a in the mice sera, as well as protein levels of IL-4, IL-6, and TSLP in ear tissues. In TNF-α/IFN-γ-stimulated keratinocytes, GM2 significantly inhibited IL-1β, IL-6, CXCL8, and CCL22 through the suppression of STAT1 phosphorylation and the nuclear translocation of NF-κB. Taken together, these results indicate that GM2 is a biologically active compound that exhibits inhibitory effects on skin inflammation and suggests that GM2 might serve as a remedy in inflammatory skin diseases, specifically on AD.

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Vectorial release of carcinoembryonic antigen induced by IFN-γ in human colon cancer cells cultured in serum-free medium

European Journal of Cancer and Clinical Oncology ◽

10.1016/0277-5379(91)90227-5 ◽

1991 ◽

Vol 27 (5) ◽

pp. 599-604 ◽

Cited By ~ 6

Author(s):

Stephen Baghdiguian ◽

Bernard Verrier ◽

Monique Roccabianca ◽

Gilbert Pommier ◽

Jacques Marvaldi ◽

...

Keyword(s):

Colon Cancer ◽

Human Colon ◽

Serum Free Medium ◽

Colon Cancer Cells ◽

Free Medium ◽

Human Colon Cancer ◽

Serum Free ◽

Ifn Γ ◽

Human Colon Cancer Cells ◽

Cells Cultured

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Augmentation of Cellular Immunity and Reduction of Influenza Virus Titer in Aged Mice Fed Lactobacillus casei Strain Shirota

Clinical and Vaccine Immunology ◽

10.1128/cdli.9.1.105-108.2002 ◽

2002 ◽

Vol 9 (1) ◽

pp. 105-108 ◽

Cited By ~ 28

Author(s):

Tetsuji Hori ◽

Junko Kiyoshima ◽

Kan Shida ◽

Hisako Yasui

Keyword(s):

Influenza Virus ◽

Oral Administration ◽

Cellular Immunity ◽

Lactobacillus Casei ◽

Control Diet ◽

Killer Activity ◽

Aged Mice ◽

Tnf Α ◽

Ifn Γ ◽

Upper Respiratory

ABSTRACT We investigated whether oral administration of Lactobacillus casei strain Shirota activates the cellular immune system and ameliorates influenza virus (IFV) titer in the nasal site in upper respiratory IFV infection by using aged mice. Natural killer activity of splenocytes and lung cells of aged mice fed an L. casei strain Shirota diet (L.casei strain Shirota group) was significantly (P < 0.01 and P < 0.05) increased compared to those fed a control diet (control group). The increases were 1.5- and 2.5-fold, respectively. In aged mice fed an XL.casei strain Shirota diet, potent induction of gamma interferon (IFN-γ) and tumor necrosis factor alpha (TNF-α), which play a very important role in excluding IFV, was evident in nasal lymphocytes. IFN-γ and TNF-α production increased 12- and 3.5-fold, respectively. In this model of upper respiratory IFV infection, the titer of IFV in the nasal washings of aged mice fed an L.casei strain Shirota diet was significantly (P < 0.05) lower than that in aged mice fed a control diet (101.6 ± 0.6 and 102.2 ± 0.5, respectively). These findings suggest that oral administration of L.casei strain Shirota activates not only systemic cellular immunity but also local cellular immunity and that it ameliorates IFV infection.

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Dissociation of macrophage cytolysis and ability to transfer anti-listeria resistance by Concanavalin A-stimulated spleen cells

Microbial Pathogenesis ◽

10.1016/0882-4010(92)90029-n ◽

1992 ◽

Vol 13 (1) ◽

pp. 25-35

Author(s):

Jon T. Roll ◽

Mary Haak-Frendscho ◽

James F. Brown ◽

Charles J. Czuprynski

Keyword(s):

Concanavalin A ◽

Spleen Cells

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Prolonged survival of mice with glioma injected intracerebrally with double cytokine—secreting cells

Journal of Neurosurgery ◽

10.3171/jns.1995.83.6.1038 ◽

1995 ◽

Vol 83 (6) ◽

pp. 1038-1044 ◽

Cited By ~ 50

Author(s):

Terry Lichtor ◽

Roberta P. Glick ◽

Tae Sung Kim ◽

Roger Hand ◽

Edward P. Cohen

Keyword(s):

Cell Line ◽

Interleukin 2 ◽

Glioma Cells ◽

Fibroblast Cell ◽

Interferon Γ ◽

Glioma Cell Line ◽

Mouse Fibroblast ◽

Spleen Cells ◽

Content Type ◽

Ifn Γ

✓ A novel approach toward the treatment of glioma was developed in a murine model. The genes for both interleukin-2 (IL-2) and interferon-γ (IFN-γ) were first transfected into a mouse fibroblast cell line that expresses defined major histocompatibility complex (MHC) determinants (H—2k). The double cytokine—secreting cells were then cotransplanted intracerebrally with the Gl261 murine glioma cell line into syngeneic C57BL/6 mice (H—2b) whose cells differed at the MHC from the cellular immunogen. The results indicate that the survival of mice with glioma injected with the cytokine-secreting allogeneic cells was significantly prolonged, relative to the survival of mice receiving equivalent numbers of glioma cells alone. Using a standard 51Cr-release assay, the specific release of isotope from labeled Gl261 cells coincubated with spleen cells from mice injected intracerebrally with the glioma cells and the cytokine-secreting fibroblasts was significantly higher than the release of isotope from glioma cells coincubated with spleen cells from nonimmunized mice. The cellular antiglioma response was mediated by natural killer/lymphokine-activated killer and Lyt-2.2+ (CD8+) cells. The increased survival of mice with glioma and the specific immunocytotoxic responses after immunization with fibroblasts modified to secrete both IL-2 and IFN-γ indicate the potential of an immunotherapeutic approach to gliomas with cytokine-secreting cells.

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CASB: A concanavalin A-based sample barcoding strategy for single-cell sequencing

10.1101/2020.10.15.340844 ◽

2020 ◽

Author(s):

Liang Fang ◽

Guipeng Li ◽

Qionghua Zhu ◽

Huanhuan Cui ◽

Yunfei Li ◽

...

Keyword(s):

Single Cell ◽

Concanavalin A ◽

Cancer Cell Line ◽

High Sensitivity ◽

Cost Effective ◽

Subtle Difference ◽

Single Cell Sequencing ◽

Minimal Sample ◽

Ifn Γ ◽

Multiple Drugs

AbstractSample multiplexing facilitates single cell sequencing by reducing costs, revealing subtle difference between similar samples, and identifying artifacts such as cell doublets. However, universal and cost-effective strategies are rather limited. Here, we reported a Concanavalin A-based Sample Barcoding strategy (CASB), which could be followed by both single-cell mRNA and ATAC (assay for transposase accessible chromatin) sequencing techniques. The method involves minimal sample processing, thereby preserving intact transcriptomic or epigenomic patterns. We demonstrated its high labeling efficiency, high accuracy in assigning cells/nuclei to samples regardless of cell type and genetic background, as well as high sensitivity in detecting doublets by two applications: 1) CASB followed by scRNA-seq to track the transcriptomic dynamics of a cancer cell line perturbed by multiple drugs, which revealed compound-specific heterogeneous response; 2) CASB together with both snATAC-seq and scRNA-seq to illustrate the IFN-γ-mediated dynamic changes on epigenome and transcriptome profile, which identified the transcription factor underlying heterogeneous IFN-γ response.

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Effect of Ninjin-Youei-To on Th1/Th2 Type Cytokine Production in Different Mouse Strains

The American Journal of Chinese Medicine ◽

10.1142/s0192415x0200034x ◽

2002 ◽

Vol 30 (02n03) ◽

pp. 215-223 ◽

Cited By ~ 11

Author(s):

Tsutomu Nakada ◽

Kenji Watanabe ◽

Guang-Bi Jin ◽

Kazuo Toriizuka ◽

Toshihiko Hanawa

Keyword(s):

Oral Administration ◽

Cytokine Production ◽

Interferon Γ ◽

Interleukin 4 ◽

Mouse Strains ◽

Enzyme Linked Immunosorbent Assay ◽

Herbal Formula ◽

Beneficial Effects ◽

Ifn Γ ◽

Th1 And Th2

Ninjin-Youei-To (NYT; Ren-Shen-Yang-Rong-Tang in Chinese) is a traditional herbal formula, which is widely used in Japan, Korea and China to modulate physiological immunity. The effects of oral administration of NYT on cytokine production from splenocytes were investigated in both C57BL/6 and BALB/c mice in which Th1 and Th2 were dominant, respectively. Splenocytes from C57BL/6 and BALB/c mice, which took NYT orally for four weeks, were cultured with anti-mouse CD3 mAb, and the supernatant was examined for cytokine production using enzyme-linked immunosorbent assay (ELISA). Administration of NYT to C57BL/6 mice, increased the production of interleukin-4 (IL-4) significantly, and slightly decreased interferon-γ (IFN-γ) production from splenocytes. In contrast, the same treatment significantly increased IFN-γ secretion from splenocytes of BALB/c mice. No remarkable changes of IL-12 production from splenocytes were observed in either strain of mice. These results suggest that oral administration of NYT ameliorates the excessive inclination of Th1 and Th2 type cytokine production, and NYT may provide a beneficial effects for the treatment of diseases caused by a skewed Th1-Th2 balance in the immune system.

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Cissampelos sympodialis Eichl. leaf extract increases the production of IL-10 by concanavalin-A-treated BALB/c spleen cells

Journal of Ethnopharmacology ◽

10.1016/s0378-8741(98)00235-9 ◽

1999 ◽

Vol 67 (1) ◽

pp. 93-101 ◽

Cited By ~ 21

Author(s):

M.R Piuvezam ◽

L.M.T Peçanha ◽

J Alexander ◽

G Thomas

Keyword(s):

Concanavalin A ◽

Leaf Extract ◽

Spleen Cells

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Activation of Vα14+ Natural Killer T Cells by α-Galactosylceramide Results in Development of Th1 Response and Local Host Resistance in Mice Infected with Cryptococcus neoformans

Infection and Immunity ◽

10.1128/iai.69.1.213-220.2001 ◽

2001 ◽

Vol 69 (1) ◽

pp. 213-220 ◽

Cited By ~ 125

Author(s):

Kazuyoshi Kawakami ◽

Yuki Kinjo ◽

Satomi Yara ◽

Yoshinobu Koguchi ◽

Kaori Uezu ◽

...

Keyword(s):

Cryptococcus Neoformans ◽

Host Resistance ◽

Peak Level ◽

Natural Killer T Cells ◽

Local Resistance ◽

Spleen Cells ◽

Th1 Response ◽

Local Host ◽

Ifn Γ ◽

Killer T Cells

ABSTRACT We examined the effect of α-galactosylceramide (α-GalCer) on the synthesis of gamma interferon (IFN-γ) and local resistance in mice infected intravenously with Cryptococcus neoformans. The level of IFN-γ in serum increased on day 3, reached a peak level on day 7, and decreased to the basal level on day 14 postinfection in mice treated with α-GalCer, while in vehicle-treated mice, no increase was detected at any time points except for a small increase on day 7. Such effects were not observed in NKT-KO mice. In CD4KO mice, minor synthesis of IFN-γ was detected on day 3 in sera but was completely abolished by day 7. The α-GalCer-induced IFN-γ production on day 3 was partially reduced in mice depleted of NK cells by treatment with anti-asialo-GM1 antibody (Ab). Spleen cells obtained from infected and α-GalCer-treated mice on day 7 produced a large amount of IFN-γ upon restimulation with live organisms, while only a marginal level of production was detected in splenocytes from infected and vehicle-treated mice. Such effects were abolished in CD4KO and NKT-KO mice. Finally, the fungal loads in the lungs and spleen on days 7 and 14 were significantly reduced in α-GalCer-treated mice compared to those in control mice. In NKT-KO mice, local resistance elicited by α-GalCer was completely abolished, although no obvious exacerbation of infection was detected. Furthermore, treatment with anti-IFN-γ monoclonal Ab mostly abrogated the protective effect of this agent. Thus, our results indicated that activation of Vα14+ NKT cells resulted in an increased Th1 response and local resistance to C. neoformans through production of IFN-γ.

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