scholarly journals Antibacterial effect of a hyperosmotic solution containing sorbate and ethanol on Enterococcus faecalis in planktonic form and as biofilm: an in vitro study

2020 ◽  
Vol 61 (2) ◽  
pp. 105-116
Author(s):  
Mónica Elizabeth Rojas Briones ◽  
Ricardo Oliva Rodríguez ◽  
Omar González Ortega ◽  
Ana María González Amaro ◽  
Jairo Mariel Cárdenas ◽  
...  
Keyword(s):  
Antibacterial Effect ◽  
Morphological Changes ◽  
Bacterial Load ◽  
In Vitro Study ◽  
Potassium Sorbate ◽  
Sedimentation Technique ◽  
Colony Forming Units ◽  
Planktonic Form ◽  
Planktonic State

The antibacterial effect of a hyperosmotic solution containing sorbate and ethanol on E. faecalis in planktonic state and in biofilm was evaluated. Three hyperosmotic solutions (HS-A, HS-B y HS-C) were obtained from different formulations of potassium sorbate and sodium chloride, which were tested as antimicrobials against planktonic forms of E. faecalis, in McFarland standards from 0.5 to 7, using the sedimentation technique and colony forming units (CFU) count. Afterwards an E. faecalis biofilm was produced in the palatal roots of upper first molars, by a static method in 21 days; subsequently they were prepared biomechanically by the Universal Protaper system, using the hyperosmotic solution B as an irrigant to evaluate the bacterial load reduction. One pre-instrumentation sample and one post-instrumentation sample were taken, and then were processed and cultivated to count CFU. Consecutively, roots were observed by scanning electron microscopy. The hyperosmotic solution had an important antibacterial effect when used against E. faecalis in planktonic state; solutions HS-A and HS-B were effective in eliminating E. faecalis up to 7 McFarland, while a statistical difference (p˂0.001) was observed in reducing the bacterial load in the biofilm, based on the log10 CFU count. The final solution tested seemed not to harm the dentinal structure and was capable of causing morphological changes to the bacterial cell consistent with a hyperosmotic shock. Thus, the solutions tested could be an option to be considered as irrigating agents; nonetheless further research is required regarding its biocompatibility.

Cytotoxic Effect of the Combination of Gemcitabine and Atorvastatin Loaded in Microemulsion on the HCT116 Colon Cancer Cells

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Mayson H. Alkhatib ◽  
Dalal Al-Saedi ◽  
Wadiah S. Backer
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Anticancer Drugs ◽  
Therapeutic Potential ◽  
Morphological Changes ◽  
In Vitro Study ◽  
Colon Cancer Cells ◽  
Apoptosis Detection ◽  
Hct116 Cells

The combination of anticancer drugs in nanoparticles has great potential as a promising strategy to maximize efficacies by eradicating resistant, reduce the dosage of the drug and minimize toxicities on the normal cells. Gemcitabine (GEM), a nucleoside analogue, and atorvastatin (ATV), a cholesterol lowering agent, have shown anticancer effect with some limitations. The objective of this in vitro study was to evaluate the antitumor activity of the combination therapy of GEM and ATVencapsulated in a microemulsion (ME) formulation in the HCT116 colon cancer cells. The cytotoxicity and efficacy of the formulation were assessed by the 3- (4,5dimethylthiazole-2-yl)-2,5-diphyneltetrazolium bromide (MTT) assay. The mechanism of cell death was examined by observing the morphological changes of treated cells under light microscope, identifying apoptosis by using the ApopNexin apoptosis detection kit, and viewing the morphological changes in the chromatin structure stained with 4′,6-diamidino-2-phenylindole (DAPI) under the inverted fluorescence microscope. It has been found that reducing the concentration of GEM loaded on ME (GEM-ME) from 5μM to 1.67μM by combining it with 3.33μM of ATV in a ME formulation (GEM/2ATV-ME) has preserved the strong cytotoxicity of GEM-ME against HCT116 cells. The current study proved that formulating GEM with ATV in ME has improved the therapeutic potential of GEM and ATV as anticancer drugs.

scholarly journals Effect of Lignocaine Concentration on Human Fibroblasts Growth in Eyes Undergoing Trabeculectomy: An in vitro Study

2018 ◽  
Vol 3 (3) ◽  
pp. 1-10 ◽  
Author(s):  
Madhuravasal Krishnan Janani ◽  
Venkatakrishnan Jaichandran ◽  
Hajib Narahari Rao Madhavan ◽  
Lingam Vijaya ◽  
Ronnie Jacob George ◽  
...  
Keyword(s):  
Culture Medium ◽  
In Vitro Model ◽  
Morphological Changes ◽  
Human Fibroblasts ◽  
Annexin V ◽  
In Vitro Study ◽  
Tenon’S Capsule ◽  
Tenon's Capsule ◽  
Vitro Model

Purpose: To evaluate the effect of lignocaine on growth and apoptosis indication of primary human Tenon’s capsule fibroblast (HTFs) in an in vitro model. Patients and Methods: Tenon’s capsule tissue obtained from patients undergoing trabeculectomy were grown in cell culture medium. The effect of different concentrations of lignocaine (0.5, 1.0, 1.5, and 2%) on the morphology and growth of the fibroblasts was studied using microscopy, cell viability, and proliferation assay, and apoptosis was detected using the FITC Annexin V Apoptosis Kit. Results: Morphological changes similar to those of apoptotic cells, including cytoplasmic vacuolation, shrinkage, and rounding were visualized in the cells treated with concentrations greater than 1.0% (i.e., 1.5, 2.0%). Though proliferation inhibition was found with all four concentrations (0.5–2.0%), the viability of cells decreased from 1.0% lignocaine. Conclusion: 0.5% lignocaine prevents proliferation of fibroblasts without causing apoptosis in vitro.

scholarly journals Antibacterial Effect of Antibiotic-Saturated Fibrin Sealant; In Vitro Study

2018 ◽  
Vol 14 (1) ◽  
pp. 12-17 ◽  
Author(s):  
Rui Ken Tan ◽  
Hyun-Su Lee ◽  
Huiling Ma ◽  
Hyup-Woo Lee ◽  
Seung-Kyu Han
Keyword(s):  
Fibrin Sealant ◽  
Antibacterial Effect ◽  
In Vitro Study ◽  
Vitro Study

scholarly journals The Antimicrobial Efficacy of four different Intracanal Medication to Microorganisms existing in the Failure of Endodontic Therapy: An in vitro study

2021 ◽  
Vol 8 (3) ◽  
pp. 01-04
Author(s):  
Jorge Paredes Vieyra ◽  
Fabian Ocampo Acosta ◽  
Francisco Javier Jiménez Enriquez ◽  
Alan Hidalgo Vargas ◽  
Ignacio Manriquez Quintana ◽  
...  
Keyword(s):  
Bacterial Load ◽  
In Vitro Study ◽  
Diffusion Test ◽  
Chemical Combination ◽  
Root Canal System ◽  
Inhibition Zones ◽  
Petri Plate ◽  
Micro Organisms ◽  
And Staphylococcus Aureus

Aim.This study aims to identify the efficacy of different intracanal medication formulations existing in end of endodontic therapy’s failure, and related to strains of Enterococcus faecalis, Pseudomonas aeruginosa, and Staphylococcus aureus in Petri plates. Materials and methods. It was used diffusion test in agar where each Petri plate with the inoculated bacteria. Perforations of approximately 4 mm deep by 5 mm in diameter were made to prepare where the intracanal drug (25 µl) to be tested. The diameters of the bacterial inhibition zones were measured and registered to each tested medication at the period of 24 hours, 48 hours, 7 and 14 days respectively. Results. All the medications promoted inhibition halos. The inhibition halos were represented in mm. A higher elimination of micro-organisms can be significantly achieved through the association of different substances in the formulation of an intra canal medication, with emphasis to Ca (OH)2 combined with nitrofurazone and magnesium oxide respectively. Conclusion. Cleaning and shaping of the root canal system associated with the chemical combination of Ca (OH) 2 with antiseptic pastes or solutions considerably reduce the bacterial load.

Antibacterial effect of diode laser on different cariogenic bacteria: An In-vitro study

2020 ◽  
Vol 23 (11) ◽  
pp. 1578
Author(s):  
TS Vinothkumar ◽  
R Apathsakayan ◽  
FM M El-shamy ◽  
HE Homeida ◽  
AIM Hommedi ◽  
...  
Keyword(s):  
Diode Laser ◽  
Antibacterial Effect ◽  
In Vitro Study ◽  
Vitro Study ◽  
Cariogenic Bacteria

scholarly journals Hypochlorite solution for root canal irrigation that lacks a chlorinated odor

2017 ◽  
Vol 11 (02) ◽  
pp. 221-225
Author(s):  
La-ongthong Vajrabhaya ◽  
Vanida Sangalungkarn ◽  
Ratchapin Srisatjaluk ◽  
Suwanna Korsuwannawong ◽  
Chareerut Phruksaniyom
Keyword(s):  
Root Canal ◽  
Antibacterial Effect ◽  
In Vitro Study ◽  
Commercial Products ◽  
Canal Irrigation ◽  
Hypochlorite Solution ◽  
Root Canal Irrigation ◽  
Chlorine Gas ◽  
Trichloroisocyanuric Acid

ABSTRACT Objectives: This is an in vitro study to develop a formulation of a hypochlorite solution for root canal irrigation that lacks a chlorinated odor. The antibacterial effect, tissue dissolution efficacy, and the cytotoxicity of the solution were assessed in cell culture and were compared with those of commercial sodium hypochlorite (NaOCl) solutions. Materials and Methods: Trichloroisocyanuric acid (TCA) was used as the source of hypochlorite ions in solution. All required properties of the NaOCl irrigant were evaluated and compared with those of original 2.5% NaOCl solutions currently in use. Results: Our results revealed that a TCA 3.5% + 1/6 Buffer-1 solution passed the short-term stability test. Moreover, no odor of chlorine gas was detected by three independent observers. The hypochlorite ion content and pH were stable over an incubation period of 4 weeks. The new solution did not differ from commercial products in terms of the dissolution property on bovine pulpal tissue (P > 0.05). Moreover, the antibacterial effect of this solution on Enterococcus faecalis did not differ from that of the commercial products (P > 0.05). In addition, our biocompatibility analysis demonstrated no difference among the tested solutions (P > 0.05). Conclusions: According to the results of all properties tested, TCA 3.5% + 1/6 Buffer-1 could be considered an option for NaOCl irrigation with the benefit of no detectable chlorine odor.

scholarly journals The Addition of Lactobacillus spp., Enrofloxacin or Doxycycline Negatively Affects the Viability of Mycoplasma bovis in Diluted Bovine Semen

Animals ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 837
Author(s):  
Ana García-Galán ◽  
Ángel Gómez-Martín ◽  
Esther Bataller ◽  
Jesús Gomis ◽  
Antonio Sánchez ◽  
...  
Keyword(s):  
In Vitro Study ◽  
Etiologic Agent ◽  
Dairy Herds ◽  
Mycoplasma Bovis ◽  
Bull Semen ◽  
Colony Forming Units ◽  
Alternative Measures ◽  
Initial Source ◽  
Lactobacillus Spp

Mycoplasma bovis is an important etiologic agent of bovine mycoplasmosis in cattle. Different transmission routes have been described, including those related to reproduction. The presence of mycoplasma in semen has led to its appearance in infection-free areas through artificial insemination (AI). Semen was recently reported to be the initial source of two M. bovis mastitis outbreaks in two closed dairy herds in Finland. This questions the effectiveness of the antimicrobials currently used in semen extenders to control the pathogens in contaminated semen. They should be re-evaluated, or alternative measures to antimicrobials should be tested to obtain M. bovis-free semen. This in vitro study aimed to assess different strategies to reduce the risk of transmission of M. bovis through AI technologies. The viability of M. bovis (PG45, NCTC 10131) in bull semen diluted (DS) in a Tris-citrate-fructose solution was tested, after the addition of enrofloxacin, doxycycline or a Lactobacillus spp.-based probiotic. The data show the susceptibility of the pathogen to the addition of 0.125 μg/mL of enrofloxacin or 0.0625 μg/mL of doxycycline and to the addition of the probiotic at a concentration of 3.24 × 106 colony forming units (CFU)/mL or 3.24 × 108 CFU/mL in DS. The Tris-citrate-fructose medium negatively affected the viability of M. bovis, although this effect was lower than that observed after the addition of the probiotic and antimicrobials (p < 0.05). Our results may support new strategies for reducing the risk of M. bovis transmission through AI.

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