scholarly journals THE DEPENDENCE OF MICROORGANISMS BIOFILM FORMATION ON THE FEATURES OF THE INFECTIOUS PROCESS

2021 ◽  
Vol 20 (2) ◽  
pp. 56-64
Author(s):  
V.Y. Ziamko ◽  
◽  
V.K. Okulich ◽  
A.M. Dzyadzko ◽  
N.E. Kolchanova ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Clinical Isolates ◽  
Microbial Biofilm ◽  
Infectious Process ◽  
Microbial Biofilms ◽  
Close Relationship ◽  
Acinetobacter Spp ◽  
Severity Of The Disease ◽  
Intensive Formation ◽  
Process Material

Objectives. To study the dependence of the formation intensity of microbial biofilms by isolates from patients on the severity and localization of the infectious process. Material and methods. The study conducted from 2016 to 2020 included 894 clinical isolates from 720 patients with infectious pathology of various localization and severety. The formation intensity of microbial biofilms by isolates received from these patients has been studied, depending on the severity and localization of the infectious process. Results. The property to form biofilms was found out in the overwhelming majority of tested isolates (86.6%). At the same time P. aeruginosa isolates had the maximum biofilm-forming ability, the weight of the microbial biofilm of which was 48.25; 30.1-70.2 mcg/well. The conducted studies have shown the dependence of biofilm formation on the severity of the disease in which isolates were isolated. A direct close relationship was found between the severity of the disease and the mass of microbial biofilm of P. aeruginosа and K. pneumoniae, a weak one - in Acinetobacter spp. (r=0.73, r=0.7 and r=0.35, p<0.05 accordingly). Conclusions. It can be assumed that intensive formation of biofilms by clinical isolates is an important factor of the aggressiveness and severity of the infectious process. Moreover, the conducted study has shown the dependence of biofilm formation on the severity of the disease in which isolates were isolated.

scholarly journals Cutaneous microbial biofilm formation as an underlying cause of red scrotum syndrome

2021 ◽  
Vol 26 (1) ◽  
Author(s):  
Theodore W. Perry
Keyword(s):  
Biofilm Formation ◽  
Disease Burden ◽  
Chronic Wounds ◽  
Gentian Violet ◽  
Symptomatic Relief ◽  
Skin Surface ◽  
Hair Follicles ◽  
Microbial Biofilm ◽  
Microbial Biofilms ◽  
Unaffected Skin

Abstract Background Red scrotum syndrome is typically described as well-demarcated erythema of the anterior scrotum accompanied by persistent itching and burning. It is chronic and difficult to treat and contributes to significant psychological distress and reduction in quality of life. The medical literature surrounding the condition is sparse, with the prevalence likely under-recognized and the pathophysiology remaining poorly understood. Formation of a cutaneous microbial biofilm has not been proposed as an underlying etiology. Microbial biofilms can form whenever microorganisms are suspended in fluid on a surface for a prolonged time and are becoming increasingly recognized as important contributors to medical disease (e.g., chronic wounds). Case presentation A 26-year-old man abruptly developed well-demarcated erythema of the bilateral scrotum after vaginal secretions were left covering the scrotum overnight. For 14 months, the patient experienced daily scrotal itching and burning while seeking care from multiple physicians and attempting numerous failed therapies. He eventually obtained complete symptomatic relief with the twice daily application of 0.8% menthol powder. Findings in support of a cutaneous microbial biofilm as the underlying etiology include: (1) the condition began following a typical scenario that would facilitate biofilm formation; (2) the demarcation of erythema precisely follows the scrotal hairline, suggesting that hair follicles acted as scaffolding during biofilm formation; (3) despite resolution of symptoms, the scrotal erythema has persisted, unchanged in boundary 15 years after the condition began; and (4) the erythematous skin demonstrates prolonged retention of gentian violet dye in comparison with adjacent unaffected skin, suggesting the presence of dye-avid material on the skin surface. Conclusion The probability that microorganisms, under proper conditions, can form biofilm on intact skin is poorly recognized. This case presents a compelling argument for a cutaneous microbial biofilm as the underlying cause of red scrotum syndrome in one patient, and a review of similarities with other reported cases suggests the same etiology is likely responsible for a significant portion of the total disease burden. This etiology may also be a significant contributor to the disease burden of vulvodynia, a condition with many similarities to red scrotum syndrome.

scholarly journals Silver-Derived Antimicrobial Coatings for the Prevention of Microbial Biofilms in Metal Pipes

2021 ◽  
Vol 232 (8) ◽  
Author(s):  
M. Vela-Cano ◽  
C. Garcia-Fontana ◽  
F. Osorio ◽  
A. González-Martinez ◽  
J. González-López
Keyword(s):  
Biofilm Formation ◽  
Antimicrobial Properties ◽  
Pilot Scale ◽  
Biological Factors ◽  
Antimicrobial Coatings ◽  
Microbial Biofilms ◽  
Physical Chemical ◽  
Silver Zeolite ◽  
Metal Pipes ◽  
Pipeline Corrosion

AbstractBiodeterioration is one of the most important processes in metal pipeline corrosion, and it can be due to physical, chemical, and biological factors. Coatings rich in silver have been used to inhibit this undesirable phenomenon. In this study, the antimicrobial properties of several silver-containing products used as a coating in pipelines were determined on a pilot scale in order to evaluate the ability of silver to inhibit biofilm formation. The results showed that the coating with silver zeolite at a concentration of 2000 mg L–1 inhibited the formation of a microbial biofilm and prevented the biodeterioration process. Therefore, from our study, it can be concluded that silver zeolite shows greater protection capacity than other silver preparations and presents advantages in relation to other silver coatings that are currently available

scholarly journals Eugenol-Functionalized Magnetite Nanoparticles Modulate Virulence and Persistence in Pseudomonas aeruginosa Clinical Strains

Molecules ◽  
2021 ◽  
Vol 26 (8) ◽  
pp. 2189
Author(s):  
Hamzah Basil Mohammed ◽  
Sajjad Mohsin I. Rayyif ◽  
Carmen Curutiu ◽  
Alexandra Catalina Birca ◽  
Ovidiu-Cristian Oprea ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Spherical Shape ◽  
Infectious Process ◽  
Phenotypic Resistance ◽  
Persistent Infections ◽  
Resistant Mutants ◽  
Subinhibitory Concentrations ◽  
Pathogenic Agents ◽  
Soluble Enzymes

Efficient antibiotics to cure Pseudomonas aeruginosa persistent infections are currently insufficient and alternative options are needed. A promising lead is to design therapeutics able to modulate key phenotypes in microbial virulence and thus control the progression of the infectious process without selecting resistant mutants. In this study, we developed a nanostructured system based on Fe3O4 nanoparticles (NPs) and eugenol, a natural plant-compound which has been previously shown to interfere with microbial virulence when utilized in subinhibitory concentrations. The obtained functional NPs are crystalline, with a spherical shape and 10–15 nm in size. The subinhibitory concentrations (MIC 1/2) of the eugenol embedded magnetite NPs (Fe3O4@EUG) modulate key virulence phenotypes, such as attachment, biofilm formation, persister selection by ciprofloxacin, and the production of soluble enzymes. To our knowledge, this is the first report on the ability of functional magnetite NPs to modulate P. aeruginosa virulence and phenotypic resistance; our data highlights the potential of these bioactive nanostructures to be used as anti-pathogenic agents.

Effect of sub-inhibitory concentrations of cefepime on biofilm formation by Pseudomonas aeruginosa

2021 ◽  
pp. 1-8
Author(s):  
Soheir A.A. Hagras ◽  
Alaa El-Dien M.S. Hosny ◽  
Omneya M. Helmy ◽  
Mounir M. Salem-Bekhit ◽  
Faiyaz Shakeel ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Clinical Isolates ◽  
Polymeric Chain ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Protein Encoding ◽  
Encoding Gene ◽  
Fimbrial Protein

This study investigated the effect of cefepime at sub-minimum inhibitory concentrations (sub-MICs) on in vitro biofilm formation (BF) by clinical isolates of Pseudomonas aeruginosa. The effect of cefepime at sub-MIC levels (½–1/256 MIC) on in vitro BF by six clinical isolates of P. aeruginosa was phenotypically assessed following 24 and 48 h of challenge using the tissue culture plate (TCP) assay. Quantitative real-time polymeric chain reaction (qRT-PCR) was employed to observe the change in expression of three biofilm-related genes, namely, a protease-encoding gene (lasA), fimbrial protein-encoding gene (cupA1), and alginate-encoding gene (algC), in a weak biofilm-producing strain of P. aeruginosa following 24 and 48 h of challenge with sub-MICs of cefepime. The BF morphology in response to cefepime was imaged using scanning electron microscopy (SEM). The TCP assay showed strain-, time-, and concentration-dependent changes in in vitro BF in P. aeruginosa following challenge with sub-MICs of cefepime, with a profound increase in strains with inherently no or weak biofilm-producing ability. RT-PCR revealed time-dependent upregulation in the expression of the investigated genes following challenge with ½ and ¼ MIC levels, as confirmed by SEM. Cefepime at sub-MICs could upregulate the expression of BF-related genes and enhance BF by P. aeruginosa clinical isolates.

scholarly journals Enhancement of pyocyanin production by subinhibitory concentration of royal jelly in Pseudomonas aeruginosa

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 14
Author(s):  
Dina Auliya Amly ◽  
Puspita Hajardhini ◽  
Alma Linggar Jonarta ◽  
Heribertus Dedy Kusuma Yulianto ◽  
Heni Susilowati
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacterial Growth ◽  
Biofilm Formation ◽  
Royal Jelly ◽  
Microtiter Plate ◽  
Multidrug Resistant ◽  
Clinical Isolates ◽  
Antibiotic Tolerance ◽  
Subinhibitory Concentration ◽  
Pyocyanin Production

Background: Pseudomonas aeruginosa, a multidrug-resistant Gram-negative bacterium, produces pyocyanin, a virulence factor associated with antibiotic tolerance. High concentrations of royal jelly have an antibacterial effect, which may potentially overcome antibacterial resistance. However, in some cases, antibiotic tolerance can occur due to prolonged stress of low-dose antibacterial agents. This study aimed to investigate the effect of subinhibitory concentrations of royal jelly on bacterial growth, pyocyanin production, and biofilm formation of P. aeruginosa. Methods: Pseudomonas aeruginosa ATCC 10145 and clinical isolates were cultured in a royal jelly-containing medium to test the antibacterial activity. Pyocyanin production was observed by measuring the absorbance at 690 nm after 36 h culture and determined using extinction coefficient 4310 M-1 cm-1. Static microtiter plate biofilm assay performed to detect the biofilm formation, followed by scanning electron microscopy. Results: Royal jelly effectively inhibited the viability of both strains from a concentration of 25%. The highest production of pyocyanin was observed in the subinhibitory concentration group 6.25%, which gradually decreased along with the decrease of royal jelly concentration. Results of one-way ANOVA tests differed significantly in pyocyanin production of the two strains between the royal jelly groups. Tukey HSD test showed concentrations of 12.5%, 6.25%, and 3.125% significantly increased pyocyanin production of ATCC 10145, and the concentrations of 12.5% and 6.25% significantly increased production of the clinical isolates. Concentrations of 12.5% and 6.125% significantly induced biofilm formation of P. aeruginosa ATCC 10145, in line with the results of the SEM analysis. Conclusions: The royal jelly concentration of 25% or higher inhibits bacterial growth; however, the subinhibitory concentration increases pyocyanin production and biofilm formation in P. aeruginosa. It is advisable to determine the appropriate concentration of royal jelly to obtain beneficial virulence inhibiting activity.

scholarly journals Molecular Epidemiology Of Clinical Isolates Of Carbapenem Resistant Acinetobacter spp.

2017 ◽  
Author(s):  
Murat Telli ◽  
Mete Eyigör ◽  
Berna Korkmazgil ◽  
Neriman Aydın ◽  
Mustafa Altay Atalay
Keyword(s):  
Molecular Epidemiology ◽  
Clinical Isolates ◽  
Carbapenem Resistant ◽  
Acinetobacter Spp

scholarly journals Molecular Investigation of Fibronectin-Binding Protein Genes and Capacity of Biofilm Production in Staphylococcus Aureus Isolated From Clinical Samples

2021 ◽  
Author(s):  
Hossein Jafari Soghondicolaei ◽  
Mohammad Ahanjan ◽  
Mehrdad Gholami ◽  
Bahman Mirzaei ◽  
Hamid Reza Goli
Keyword(s):  
Staphylococcus Aureus ◽  
Biofilm Formation ◽  
Colorimetric Assay ◽  
Binding Protein ◽  
Clinical Isolates ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Biofilm Production ◽  
Fibronectin Binding Protein ◽  
Fibronectin Binding

Abstract Biofilm production increases Staphylococcus aureus resistance to antibiotics and also host defense mechanisms. The current study aims to evaluate the biofilm formation by S. aureus and to determine the prevalence of fibronectin-binding protein genes, also its correlation with drug resistance. In this study, 100 clinical isolates of S. aureus were collected. The antibiotic susceptibility pattern of the isolates was evaluated by the disk agar diffusion method. The ability of biofilm formation in the studied isolates was also determined by microplate colorimetric assay. Then, all isolates were screened by polymerase chain reaction for the fnbA and fnbB genes. Out of 100 clinical isolates of S. aureus, the highest and lowest antibiotic resistance rates were against penicillin (94%) and vancomycin (6%). Thirty-two cases were found to be multi-drug resistant (MDR) among the all strains. The ability of biofilm production was observed in 89% of the isolates. The PCR results showed that the prevalence of fnbA and fnbB genes were 91% and 17%, respectively. Moreover, 100% and 21.8% of the MDR strains harbored the fnbA and fnbB genes respectively. The ability to form biofilm in MDR isolates of S. aureus is more than non-MDR isolates, especially fnbA positive ones. As the bacteria in the biofilm are difficult to kill by antibiotics, attention to the removal or control of the biofilm production seems to be necessary.

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