scholarly journals Efficacy of partial purified bacteriocin of Pseudomonas aeruginosa on Methicillin-resistant Staphylococcus aureus biofilm

2019 ◽  
Vol 9 (4-A) ◽  
pp. 438-441
Author(s):  
Arumugam Thangarasu ◽  
Dhanam Selvam ◽  
Sampath Gattu ◽  
Kayalvizhi Nagarajan
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Culture Supernatant ◽  
Chronic Infections ◽  
Antibiotic Resistant ◽  
Methicillin Resistant ◽  
Marked Inhibition ◽  
Ft Ir ◽  
Resistant Microorganism

Biofilms are microbial communities that cause serious chronic infections in the environment by enhancing antimicrobial resistance. Bacteria in the biofilm can be up to a thousand times more resistant to antibiotics than the same bacteria circulating in a planktonic state. The emergence of antibiotic-resistant microorganism has led to the exploration of different therapeutic agents like ribosomally synthesized microorganism peptides referred to as bacteriocins. In this study, bacteriocin producing bacteria Pseudomonas aeruginosa isolated from a soil sample. It was found to be effective against Methicillin-resistant Staphylococcus aureus (MRSA). Furthermore the bacteriocin was partial purified by ammonium sulfate, the precipitate has highly effective against MRSA (400AU/mL). MRSA cells were treated with precipitated culture supernatant of P. aeruginosa TA6 was analyzed by FT-IR. The treated and untreated MRSA showed band variations at 682.59 and 3442.15cm-1 corresponding to the alkyl and amide group respectively. Bacteriocin showed marked inhibition activity against the biofilm of MRSA. About 0.05% and 0.02% attachment of biofilm was observed in the presence of 1X MIC (10 μg/mL) and 2X MIC (20 g/mL) respectively. Our results recommend that bacteriocins that make stable pores on biofilm cells are extremely potent for the treatment of MRSA biofilm infections.

scholarly journals Multimicrobial Pneumonia during the Early Stages of a Global Pandemic

2021 ◽  
Vol 8 (6) ◽  
Author(s):  
Jalal H ◽  
◽  
Henriksen G ◽  
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Acute Infection ◽  
Standard Of Care ◽  
Antibiotic Use ◽  
Lung Parenchyma ◽  
Community Acquired Pneumonia ◽  
Methicillin Resistant ◽  
Global Pandemic

Community-acquired pneumonia is an acute infection of lung parenchyma which causes local and systemic inflammatory changes via cytokines. Several bacteria and viruses are responsible for this type of pneumonia, and the most common bacterial cause is Streptococcus pneumoniae. The classic symptoms are cough, fever, and pleuritic chest pain. In the Winter of 2020, a new strain of coronavirus known as SARS-CoV-2 spread throughout the world and was responsible for a global pandemic that transformed the way we live our lives. A 93-year old female presented to the hospital with respiratory distress and was found to have not only COVID-19 pneumonia but also superimposed Methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa pneumonia. Following the most up-to-date guidelines, she was determined to have community-acquired pneumonia. Methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa are uncommon causes of communityacquired pneumonia. She was treated with the standard of care at the time, which included vancomycin, piperacillin-tazobactam, and hydroxychloroquine. This case highlights the rarity of this specific presentation of community acquired pneumonia in regards to microbial etiology. It showcases that patients may develop certain diseases despite not having any risk factors. A major takeaway point is that apt decision making is a critical and time sensitive matter when determining whether a bacterial co-infection is present since it can affect patient outcomes. Since co-infections are relatively infrequent, antibiotic use in COVID-19 positive patients needs to be tailored accordingly. At the same time, it is crucial to keep in mind that co-infections are associated with increased severity of COVID-19 as well as poorer outcomes.

scholarly journals Diabetic Foot Infections: Local Prevalence of and Case–Control Study of Risk Factors for Methicillin-Resistant Staphylococcus aureus and Pseudomonas aeruginosa

2020 ◽  
Vol 7 (10) ◽  
Author(s):  
Justin J Kim ◽  
Alison Lydecker ◽  
Rohini Davé ◽  
Jacqueline T Bork ◽  
Mary-Claire Roghmann
Keyword(s):  
Risk Factors ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Diabetic Foot ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Case Control Study ◽  
Case Control ◽  
Methicillin Resistant ◽  
Diabetic Foot Infections ◽  
Control Study

Abstract We identified deep diabetic foot infections by culture and conducted a case–control study examining the risk factors for moderate to severe methicillin-resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa (PsA) diabetic foot infections. Our MRSA prevalence was lower than literature values; PsA was higher. Gangrene may be predictive of Pseudomonas infection.

scholarly journals In Vitro Comparison of Antibacterial and Antibiofilm Activities of Selected Fluoroquinolones against Pseudomonas aeruginosa and Methicillin-Resistant Staphylococcus aureus

Pathogens ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 12 ◽  
Author(s):  
Majed Masadeh ◽  
Karem Alzoubi ◽  
Wesam Ahmed ◽  
Aisha Magaji
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Culture Collection ◽  
Strain Atcc ◽  
Inhibitory Effects ◽  
Planktonic Cells ◽  
Methicillin Resistant ◽  
Insight Into

An in vitro overview of the inhibitory effects of selected fluoroquinolones against planktonic and biofilm cells of the methicillin-resistant Staphylococcus aureus (MRSA) strain American type culture collection (ATCC) 43300 and the Pseudomonas aeruginosa strain ATCC 27853 was carried out. Biofilm cells of both strains were less susceptible to the selected antibiotics than their planktonic counterparts. In addition, certain antibiotics were more effective against biofilm cells, while others performed better on the planktonic cells. Against P. aeruginosa, ciprofloxacin was the most potent on both planktonic and biofilm cells, whereas ofloxacin was the least potent on both biofilm and planktonic cells. Moxifloxacin and gatifloxacin were the most potent against both planktonic and biofilm MRSA bacteria, however, not in the same order of activity. Norfloxacin was the least active when tested against both planktonic and biofilm cells. The results of this work are expected to provide insight into the efficacy of various fluoroquinolones against MRSA and Pseudomonas aeruginosa biofilms. This study could form the basis for future clinical studies that could recommend special guidelines for the management of infections that are likely to involve bacteria in their biofilm state.

scholarly journals SURVEILLANCE OF BACTERIA METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS AND PSEUDOMONAS AERUGINOSA IN PATIENTS ADMITTED TO ORTHOPEDIC DEPARTMENT IN A TERTIARY REFERRAL HOSPITAL

2018 ◽  
Vol 11 (6) ◽  
pp. 381
Author(s):  
Pulin Bihari Das ◽  
Monali Priyadarshini Mishra ◽  
Siba Narayan Rath
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Surgical Site Infections ◽  
Macconkey Agar ◽  
Tertiary Referral ◽  
Tertiary Referral Hospital ◽  
Methicillin Resistant ◽  
Fungal Strains ◽  
Mrsa Strains

Objective: Methicillin-resistant Staphylococcus aureus (MRSA) strains have emerged independently in diverse geographic zones and MRSA and Pseudomonas aeruginosa cause surgical site infections. Nosocomial surveillance in orthopedic surgery wards of the hospital for 16 months is presented.Methods: A total of 621 wound swabs were cultured on blood and MacConkey agar plates for bacteria and Sabouraud dextrose agar for fungi.Results: From 468 bacterial colonies, 98 MRSA and 74 P. aeruginosa strains and 41 fungal strains were isolated, and fungal strains were 13 strains of Aspergillus niger, and 28 strains of Candida albicans. P. aeruginosa and S. aureus strains were susceptible to antibiotics tobramycin, ciprofloxacin, piperacillin, vancomycin, levofloxacin, and amoxyclav. Similarly, A. niger and C. albicans were susceptible to antifungals, amphotericin B (AMB), liposomal AMB, itraconazole, voriconazole, posaconazole, and caspofungin.Conclusion: Isolated MRSA strains were resistant to presently used common antibiotics, which attribute to the leading causatives of post-operative infection in orthopedic wounds, specifically.

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