scholarly journals Complex effect of caffeine and dioxidine on behavioral responses in mice in Porsolt test

2020 ◽  
Vol 24 (4) ◽  
pp. 315-324
Author(s):  
N. A. Durnova ◽  
A. Yu. Karetnikova ◽  
D. S. Isaev ◽  
A. R. Klantsataya ◽  
A. S. Sheremetyeva
Keyword(s):  
Behavioral Responses ◽  
Antidepressant Activity ◽  
Repeated Administration ◽  
Antidepressant Effect ◽  
Control Group ◽  
Porsolt Test ◽  
Combined Use ◽  
Combined Administration ◽  
Active Swimming ◽  
Experiment Duration

Relevance. In light of the popularization of the use of caffeine-containing products, the question of the combined use of caffeine with substances exhibiting a toxic effect remains open. The doses of caffeine, which have a pronounced antidepressant effect, are also insufficiently studied. The aim of the study was to study the effect of repeated administration of caffeine and dioxidine on the behavioral responses of mice in the Porsolt test. Materials and methods. The experiment was carried out on 36 outbred male mice, divided into 6 groups. Experimental groups for 15 days of the study received caffeine at a dose of 40 mg/kg (first) or 100 mg/kg (second), dioxidine at a dose of 200 mg/kg (third), together with caffeine 40 mg/kg or 100 mg/kg, and dioxidine (fourth and fifth groups, respectively). The animals of the control group were injected with saline. To study the behavior, the Porsolt test was carried out, evaluating the following indicators on the 1st, 8th and 15th days of the experiment: the total time of immobilization, active swimming, climb, the number of grooming and shaking off acts. Results . The administration of caffeine at a dose of 40 mg/kg caused an increase in the time of active swimming and a decrease in the duration of immobilization on the 8th and 15th days. When caffeine was used at a dose of 100 mg/kg, an increase in the time of active swimming was noted with a single exposure, with an experiment duration of 8-15 days, an increase in the duration of immobilization was observed. Dioxidine caused a significant decrease in the time of active swimming and an increase in the duration of immobilization during all days of the experiment. The combined use of caffeine (40 mg/kg and 100 mg/kg) and dioxidine on the 1st day led to a decrease in immobilization and the time of active swimming. In both groups, 100 % animal mortality was observed by the 15th day. Conclusion. The results of the study indicate the presence of an antidepressant effect in caffeine at a dose of 40 mg/kg on the 8th and 15th days of the experiment and the absence of this effect in caffeine at a dose of 100 mg/kg with a duration of administration of 8-15 days. The use of dioxidine led to the absence of antidepressant activity and the presence of the opposite effect. The combined administration of caffeine (40 mg/kg and 100 mg/kg) and dioxidine led to 100 % mortality in the experimental groups by the 15th day of the experiment

scholarly journals Preclinical Screening of Antidepressant Activity of Formulated Sertraline Hydrochloride-Loaded Solid Lipid Nanoparticles in Rats

2021 ◽  
pp. 134-138
Author(s):  
Sachin R. Hangargekar ◽  
Pradeep K. Mohanty ◽  
Janki Prasad Rai
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Lipid Nanoparticles ◽  
Antidepressant Effect ◽  
Control Group ◽  
Solid Lipid ◽  
Test Formulation ◽  
Sertraline Hydrochloride ◽  
Swimming Test

Objective: The main goal of our study was to investigate the antidepressant activity of Formulated Sertraline hydrochloride-loaded Solid Lipid Nanoparticles (SLNPs) by using a rat forced swimming test (FST) and tail suspension test (TST). Materials and Methods: Animals were divided into three groups, consisting of six rats in each group. Out of these,  one  group  served  as  control  that received distilled water, second group was standard received Sertraline  HCl  (20  mg/kg  intranasal)  and  third  group  was  received  test formulation (SLNPs 50 mg/kg intranasal). To assess the effect of SLNPs on immobility activity through FST and TST were used to take as a measure of antidepressant activity. Results: SLNPs reduced the immobility duration in TST as well as in FST. In both methods, there was a statistical significant decrease in immobility of SLNPs group when compared to the control group. Conclusion: The results suggested that SLNPs produced significant antidepressant effect in rats which was comparable with control group and standard Sertraline HCl group animals.

scholarly journals The study of the effects of dipeptide mimetic of a brain derived neurotrophic factor with antidepressant activity during prolonged use and after withdrawal

2018 ◽  
Vol 16 (1) ◽  
pp. 28-33
Author(s):  
Taisiya L Garibova ◽  
Valentina A Krayneva ◽  
Elena A Valdman ◽  
Tatyana A Gudasheva ◽  
Svetlana O Kotelnikova ◽  
...  
Keyword(s):  
Neurotrophic Factor ◽  
Antidepressant Activity ◽  
Brain Derived Neurotrophic Factor ◽  
Antidepressant Effect ◽  
Prolonged Administration ◽  
Elevated Plus Maze Test ◽  
Porsolt Test ◽  
Plus Maze ◽  
Term Administration

The antidepressant activity of the GSB-106, dipeptide mimetic of the 4th loop of BDNF, during long-term use was studied in doses of 1.0 mg/kg and 5 mg/kg for. When comparing antidepressant activity in the “forced swimming Porsolt test” on the 14th and 28th day of administration, it was established that the antidepressant effect did not change. It was shown that a withdrawal of prolonged administration for 28 days did not lead to an increase in anxiety in the “elevated plus-maze test” and to the development of disturbances in the exploratory behavior of animals and motor activity in the “open field” test. It was concluded that during long-term administration of GSB-106 the tolerance for the main antidepressant effect did not developed, and cessation of long-term administration of GSB-106 did not lead to the development of withdrawal syndrome. (For citation: Garibova TL, Krayneva VA, Valdman EA, et al. The study of the effects of dipeptide mimetic of a brain derived neurotrophic factor with antidepressant activity during prolonged use and after withdrawal. Reviews on Clinical Pharmacology and Drug Therapy. 2018;16(1):28-33. doi: 10.17816/RCF16128-33).

scholarly journals Anxiolytic and antidepressant effect of zinc on rats and its impact on general behavioural parameters

2013 ◽  
Vol 70 (4) ◽  
pp. 391-395 ◽  
Author(s):  
Janko Samardzic ◽  
Kristina Savic ◽  
Nemanja Stefanovic ◽  
Radomir Matunovic ◽  
Dragana Baltezarevic ◽  
...  
Keyword(s):  
Locomotor Activity ◽  
Forced Swim Test ◽  
Essential Element ◽  
Repeated Administration ◽  
Antidepressant Effect ◽  
Control Group ◽  
Single Application ◽  
Antidepressant Effects ◽  
Immobility Time ◽  
Significant Difference

Background/Aim. Zinc is an essential element which has considerable interaction with gamma-aminobutyric acid A type receptors (GABAA) and glutamate receptors in the central nervous system (CNS). It is believed that zinc acts as a potent inhibitor of glutamate N-methyl-D-aspartate (NMDA) receptors, and binding to structurally specific site on the GABAA receptor leads to inhibition of GABA dependent Cl-pass. The aim of our research was to test the anxiolytic and antidepressant effects of zinc after single application and its influence on general behavioural parameters after repeated administration. Methods. Male Wistar rats were treated with increasing doses of zinc histidine dehydrate (10, 20, 30 mg/kg, i.p.). To determine anxiolytic and antidepressant properties of zinc two models were used: elevated plus maze (EPM) and forced swim test (FST). Behavioural parameters (stillness and mobility) were, also, recorded after single and repeated administration of active substance. Results. Testing animals in the EPM showed a statistically significant difference as follows: dose of 20 mg/kg significantly increased the time animals spent in open arms, indicating an acute anxiolytic effect, while doses of 30 mg/kg significantly reduced the time in the open arms, indicating a potentially anxiogenic effect. Testing the animals by FST showed a statistically significant difference in immobility time of animals treated with the lowest applied (10 mg/kg) and highest applied (30 mg/kg) doses of zinc, compared to the control group. The first day of testing behavioral parameters showed the tendency to increase locomotor activity of the animals with the lowest dose of zinc (10 mg/kg), while the following day revealed a reduced activity with the highest dose applied (30 mg/kg). Conclusion. Zinc has important effects on the CNS: After single application, in all doses zinc showed antidepressant effects. The effects of zinc on anxiety and locomotor activity showed dose-dependent bidirectional effects.

scholarly journals Fruits of Crataegus submollis: study of pharmacological activity of the preparations

2019 ◽  
Vol 4 (3) ◽  
pp. 69-72
Author(s):  
V. A Kurkin ◽  
E. N Zaitceva ◽  
O. E Pravdivtseva ◽  
A. V Kurkina ◽  
V. V Stenyaeva
Keyword(s):  
Antidepressant Activity ◽  
Threshold Dose ◽  
Antidepressant Effect ◽  
Control Group ◽  
Diuretic Activity ◽  
Water Control ◽  
Excretory Function ◽  
Motion Activity ◽  
Liquid Extract ◽  
The Impact

Objectives - to perform a comparative study of the antidepressant action and diuretic activity of liquid extract and decoction of the fruits of Crataegus submollis Sarg. Material and methods. The study of the antidepressant activity was performed using the behavioral despair test. The diuretic activity of the liquid extract and decoction from the Crataegus submollis fruits at a dose of 100 pl/kg was evaluated in long-term experiments. Results. The study revealed the impact of the liquid extract of Crataegus submollis fruits, at a dose of 100 pl/kg, on the motion activity of animals: it increased by 45% if compared to the water-alcohol control group, and equivalent to comparator amitriptyline at a threshold dose of 5 mg/kg. While the diuretic activity of the the liquid extract was not registered. At the same time, the decoction of the fruits of Crataegus submollis, at a dose of 100 pl/kg, did not show antidepressant properties, when compared to water control. It was found that animals from the experimental group relative to water control had a statistically significant increase in renal excretion of water, sodium, potassium and creatinine, equivalent to comparators - furosemide at a threshold dose of 1 mg/kg (4 hours experiment), and hydrochlorothiazide at a mean therapeutic dose of 20 mg/kg (24 hours experiment). Conclusion. The liquid extract from fruits of Crataegus submollis at a dose of 100 |rl/kg showed an antidepressant effect, comparable to the effect of amitriptyline at a dose of 5 mg/kg. An expressed stimulating activity on the kidneys excretory function during the 4 and 24 hours of the experiment is characteristic of the decoction from fruits of Crataegus submollis at a dose of 100 |rl/kg. The fruits of Crataegus submollis Sarg. are promising medicinal plant raw materials.

scholarly journals Antidepressant like activity of Buspirone but not ondensetron in combination with Fluoxetine or Desipramine in mice

2021 ◽  
Vol 10 (6) ◽  
pp. 621
Author(s):  
Veena Verma ◽  
Biswadeep Banerjee ◽  
Ashish K. Mehta
Keyword(s):  
Gradual Increase ◽  
Chronic Mild Stress ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Antidepressant Effect ◽  
Control Group ◽  
Mild Stress ◽  
Sucrose Consumption ◽  
Immobility Time ◽  
Immobility Period

Background: The involvement of one or more 5-HT receptor sub-types in the pathophysiology of depression is still unclear. The study was performed to investigate the effect of ondansetron and buspirone on depression, and their interaction with fluoxetine or desipramine.Methods: The mice were administered ondansetron, buspirone alone and in combinations with fluoxetine or desipramine for 21 days, and the antidepressant effect was assessed by the immobility period and the sucrose consumption, on the tail suspension test (TST) and the chronic mild stress (CMS) models, respectively.Results: Both ondansetron and buspirone when given alone demonstrated slight non-significant decrease in the immobility time. Ondensetron when given in combination with fluoxetine (10 mg/kg; i.p.) and desipramine (15 mg/kg; i.p.), showed significant decrease in immobility time in comparison to the control group only. On the other hand, both the combinations of buspirone, either with fluoxetine or desipramine showed significant decrease in the immobility time when compared to the respective group. In CMS, the fluoxetine, desipramine, ondansetron, and buspirone showed gradual increase in the sucrose consumption, at the end of 4th, 5th, and 6th week, but the significant effect was observed only at the end of 6th week, as compared to the control. The combination of buspirone with desipramine but not with fluoxetine showed significant increase in sucrose consumption when compared to respective group.Conclusions: Therefore, the study indicates that both buspirone and ondansetron have a potential antidepressant like action, although buspirone has shown better antidepressant activity than ondansetron as observed in various combination groups.

scholarly journals ANTI-DEPRESSANT EFFECT OF CEFTRIAXONE IN FORCED SWIMMING TEST AND IN TAIL SUSPENSION TEST IN MICE

2016 ◽  
Vol 8 (11) ◽  
pp. 191 ◽  
Author(s):  
Ajoy Borah ◽  
Binita Singha ◽  
Swopna Phukan
Keyword(s):  
Forced Swimming Test ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Forced Swimming ◽  
Antidepressant Effect ◽  
Tail Suspension ◽  
Neuroprotective Effects ◽  
The Central Nervous System ◽  
Swimming Test ◽  
Suspension Test

Objective: Depression is a major psychiatric disorder affecting nearly 350 million people worldwide and imposes a substantial health burden on the society. Ceftriaxone has demonstrated neuroprotective effects in animals. It has also undergone trials as a treatment option for amyotrophic lateral sclerosis. This study was therefore undertaken to evaluate the antidepressant-like effect of ceftriaxone in mice.Methods: Ceftriaxone was administered at three different doses (0.130, 0.195 and 0.260g/kg) to Swiss albino mice of either sex by intra peritoneal (i. p.) route. The period of immobility in control and drug-treated mice were recorded in forced swimming test (FST) and tail suspension test (TST). The antidepressant effect of ceftriaxone indicated by the decrease in duration of immobility was compared to that of fluoxetine (0.020 g/kg, i. p.).Results: Ceftriaxone decreased the duration of immobility in mice. It showed a significant dose-dependent antidepressant effect. The antidepressant effect of 0.260g/kg of ceftriaxone was comparable to that of fluoxetine in the TST but not in the FST.Conclusion: The results of the present study indicate antidepressant activity of Ceftriaxone. The study shows that ceftriaxone has additional action on the central nervous system other than neuroprotection. Ceftriaxone therapy in cases of encephalomeningitis and in various cases of hemorrhages in the brain can, therefore, prevent the development of depression in future

scholarly journals Evaluation of antidepressant activity of tramadol in comparison with imipramine in Swiss albino mice

2017 ◽  
Vol 6 (3) ◽  
pp. 695
Author(s):  
Chiranjeevi Bonda ◽  
Sudhir Pawar ◽  
Jaisen Lokhande
Keyword(s):  
Forced Swim Test ◽  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Antidepressant Effect ◽  
Tail Suspension ◽  
Swim Test ◽  
Forced Swim ◽  
Group I ◽  
Immobility Time ◽  
Suspension Test

Background: The aim of the study was to evaluate the antidepressant effect of opioid analgesic tramadol using forced swim test and tail suspension test models.Methods: The antidepressant effect was assessed by recording the immobility time in Forced swim test (FST) and Tail suspension test (TST). The mice were randomly divided into five groups. Mice belonging to group I was given normal saline (0.1ml/kg) which acted as control. Group II received imipramine (15mg/kg) considered as the standard drug tramadol was given in graded dose (10, 20 and 40 mg/kg) to mice of groups III, IV, V respectively. All drugs were administered intraperitoneally for seven successive days; test was done on 7th day.Results: Tramadol and Imipramine showed antidepressant activity when compared to control. There is dose dependent increase in antidepressant activity of tramadol. The antidepressant activity of imipramine was significantly (P<0.05) more than tramadol at dose 10 and 20 mg/kg but antidepressant activity with tramadol 40mg/kg was comparable to imipramine treated mice.Conclusions: The results of this study indicated the presence of antidepressant activity of tramadol at 40mg/kg.

scholarly journals Effects of the combined use of glutamine and growth hormone in the intestinal adaptation after massive resection of the small bowel in rats

2005 ◽  
Vol 20 (5) ◽  
pp. 382-389 ◽  
Author(s):  
Joaquim M. Spadoni ◽  
José Eduardo de Aguilar-Nascimento ◽  
Maria H.G. Gomes da Silva ◽  
Bruno Spadoni-Neto ◽  
Priscila Arruda Thulio F. Batista da Costa ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Small Bowel ◽  
Dna Content ◽  
Intestinal Adaptation ◽  
Resected Specimen ◽  
Control Group ◽  
Jejunal Mucosa ◽  
Wall Width ◽  
Massive Resection ◽  
Combined Use

PURPOSE: The aim of this study was to investigate the effects of the combined use of glutamine (GL) and growth hormone (GH) in the intestine of rats submitted to 80% small bowel resection. METHODS: [24] Twenty four Wistar rats were randomized to receive either a standard rat chow - control group (CG, n=12) or the same diet added to 4% glutamine - GL-GH group (n=12) after 80% enterectomy. The latter group received subcutaneously 0,6UI/day of GH. Groups of six rats in each group were killed on the 5th and 14th days. The following variables were studied: body weight, mucosal weight, histomorphometry and DNA content in the resected specimen and in the adapted intestines after necropsy. RESULTS: All animals lost weight stabilizing after the 5th PO day in both groups. There was not any statistical difference in the mucosal weight associated to groups and dates. However, ileal mucosal weight decreased from basal to final results when compared to jejunal mucosa (p= 0.02). The DNA content increased from the initial to the final results (p <0.001) in both groups, though, this increase was greater in GL-GH animals (CG = 0.53 [95% CI, 0.44-0.62] g/cm-1 vs. GL-GH= 0.85 [95%CI, 0.76-0.94] g/cm-1; p<0.01), especially at the 14th day. Ileal DNA content was significantly greater than jejunal (p=0.01). There was a significant increase in the intestinal wall width and crypt depth in the control group (p<0.01). CONCLUSION: Gut adaptation after massive resection is improved with the combined use of glutamine and GH.

scholarly journals AN EXPERIMENTAL STUDY EVALUATING THE INFLUENCE OF QUERCETIN ON MONOSODIUM GLUTAMATE-INDUCED DEPRESSION IN SWISS ALBINO MALE MICE

2019 ◽  
pp. 292-294
Author(s):  
SRIRAM BS ◽  
RAVICHANDRA V
Keyword(s):  
Experimental Study ◽  
Behavioral Disorders ◽  
Neurotrophic Factor ◽  
Monosodium Glutamate ◽  
Antidepressant Activity ◽  
Brain Derived Neurotrophic Factor ◽  
Control Group ◽  
Male Mice ◽  
Significant Difference

Objective: The objective of the study was to evaluate the antidepressant activity of quercetin in monosodium glutamate (MSG) model of depressed male mice. Methods: MSG was administered (500 mg/kg) to different groups of albino male mice daily for 21 days to induce depression. The interventions (Quercetin and imipramine) were started on day 9th and continued till 21st day. On 23rd day, mice are sacrificed, hippocampus and amygdala supernatant are subjected for analysis. p<0.05 was considered as statistically significant. Results: There was a statistically significant reduction in interleukin (IL)-6 levels in animals treated with quercetin and imipramine compared to control group (p<0.001). There was also a statistically significant increase in brain-derived neurotrophic factor (BDNF) levels in quercetin with MSG groups (p<0.05) and imipramine with MSG groups (p<0.01). There was no statistically significant difference in IL-6 and BDNF levels between the groups of animals treated with quercetin (100 mg/kg) and imipramine (10 mg/kg) alone. Conclusion: Quercetin appeared to have an antidepressant activity. More extensive research is required to substantiate and elucidate the role of quercetin in behavioral disorders such as depression.

scholarly journals Antidepressant Potential of Lotus corniculatus L. subsp. corniculatus: An Ethnobotany Based Approach

Molecules ◽  
2020 ◽  
Vol 25 (6) ◽  
pp. 1299
Author(s):  
Fatma Tuğçe Gürağaç Dereli ◽  
Haroon Khan ◽  
Eduardo Sobarzo-Sánchez ◽  
Esra Küpeli Akkol
Keyword(s):  
Antidepressant Activity ◽  
Tail Suspension Test ◽  
Lotus Corniculatus ◽  
Antidepressant Effect ◽  
In Vivo Test ◽  
Aerial Parts ◽  
Test Models ◽  
Bioassay Guided Fractionation

As a Turkish traditional medicinal plant, aerial parts of Lotus corniculatus L. subsp. corniculatus (Fabaceae) are used as a painkiller, antihemoroidal, diuretic and sedative. In this study, the antidepressant potential of the plant has been attempted to clarify. Extracts with water, n-Hexane, ethyl acetate, and methanol were prepared respectively from the aerial parts. Antidepressant activity of the extracts were researched by using three different in vivo test models namely a tail suspension test, antagonism of tetrabenazine-induced hypothermia, ptosis, and suppression of locomotor activity and forced swimming test on male BALB/c mice and in vitro monoamine oxidase (MAO)-A and B inhibition assays. The results were evaluated through comparing with control and reference groups, and then active compounds of the active extract have been determined. Bioassay-guided fractionation of active fraction led to the isolation of three compounds and structures of the compounds were elucidated by spectroscopic methods. The data of this study demonstrate that the MeOH extract of the aerial parts of the plant showed remarkable in vivo antidepressant effect and the isolated compounds medicarpin-3-O-glucoside, gossypetin-3-O-glucoside and naringenin-7-O-glucoside (prunin) from the active sub-fractions could be responsible for the activity. Further mechanistic and toxicity studies are planned to develop new antidepressant-acting drugs.

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