ABSTRACTBacillus thuringiensissubsp.israelensisis a bacterium producing crystals containing Cry and Cyt proteins, which are toxic for mosquito larvae. Nothing is known about the interaction between crystal toxins and decaying leaf litter, which is a major component of several mosquito breeding sites and represents an important food source. In the present work, we investigated the behavior ofB. thuringiensissubsp.israelensistoxic crystals sprayed on leaf litter. In the presence of leaf litter, a 60% decrease in the amount of Cyt toxin detectable by immunology (enzyme-linked immunosorbent assays [ELISAs]) was observed, while the respective proportions of Cry toxins were not affected. The toxicity of Cry toxins towardAedes aegyptilarvae was not affected by leaf litter, while the synergistic effect of Cyt toxins on allB. thuringiensissubsp.israelensisCry toxins was decreased by about 20% when mixed with leaf litter. The toxicity of two commercialB. thuringiensissubsp.israelensisstrains (VectoBac WG and VectoBac 12AS) and a laboratory-producedB. thuringiensissubsp.israelensisstrain decreased by about 70% when mixed with leaf litter. Taken together, these results suggest that Cyt toxins interact with leaf litter, resulting in a decreased toxicity ofB. thuringiensissubsp.israelensisin litter-rich environments and thereby dramatically reducing the efficiency of mosquitocidal treatments.