scholarly journals Detection of Transovarial Transmission on Aedes sp. in Gombong Kebumen Central Java

2019 ◽  
Vol 11 (2) ◽  
pp. 107-112
Author(s):  
Siwi Pramatama Mars Wijayanti ◽  
Devi Octaviana ◽  
Arnika Dwi Asti
Keyword(s):  
Dengue Virus ◽  
Endemic Area ◽  
Transovarial Transmission ◽  
Next Generation ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Descriptive Research ◽  
Central Java ◽  
Dengue Transmission ◽  
Study Sites

Abstract. Gombong Subdistrict is one of dengue-endemic area in Kebumen Regency, Central Java. The high number of dengue cases in this area raises the question of whether there has been transovarial transmission occured from the Aedes sp. to their eggs. Transovarial transmission could be dangerous because the next generation of mosquitoes can directly become competent vectors as transmitters of the dengue virus (DENV). The purpose of this study is to detect dengue virus transovarial transmission in Aedes sp. in Gombong Subdistrict. This is a descriptive research in Gombong, Semanding, and Kali Tengah villages, Gombong Sub District. A total of 300 houses, 100 houses from each village were selected in this study. There were 600 Oviposition traps (ovitraps) were installed both inside and outside of houses for 6 days. Ovitraps were calculated by its Ovitrap Index (OI). Detection of transovarial transmission was carried by rearing field mosquitoes to Filial 1 then identified by RT-PCR assay. This study showed that OI in the three villages was higher in outdoor compared to indoor positions. All tested samples were negative DENV, indicated that there were no transovarial transmission occured at the study sites. Transmission in these study areas might still through horizontal mechanism transmission by mosquito bites. Although there is no transovarial transmission, awareness of dengue transmission must be continued by eradicating of mosquito nests such as 3M plus activities on a regular basis

scholarly journals Epidemiological Survey and Screening Strategy for Dengue Virus in Blood Donors from Yunnan Province

2020 ◽  
Author(s):  
LING LI ◽  
YING LI ◽  
Shaofang Lu ◽  
Jing Dong ◽  
Haixia Xu ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Prevalence Rate ◽  
Screening Strategy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Igm Antibodies ◽  
Blood Center ◽  
Nonstructural Protein 1 ◽  
Elisa Testing

Abstract BACKGROUND Dengue virus (DENV) can be transmitted through blood transfusion. DENV was not screened regularly in Xishuangbanna Blood Center. This study was conducted in Xishuangbanna Blood Center with an attempt to develop DENV screening strategies in one of China’s high-incidence areas.METHODS Blood samples were collected randomly between June 2019 and August 2019. These samples were first screened for dengue IgG and IgM antibodies using enzyme-linked immunosorbent assay (ELISA). All reactive samples and some randomly-chosen non-reactive samples were used to detect DENV RNAs using real time polymerase-chain-reaction (RT-PCR) assay. After RT-PCR assay, these samples were further tested for soluble nonstructural protein 1 (NS1) using colloidal gold method. The demographic data of DENV positive donors were collected.RESULTS A total of 2,254 donor samples were collected and tested for dengue IgG and IgM antibodies by ELISA between June 2019 and August 2019. ELISA testing revealed that 598 donor samples were anti-IgG and/or anti-IgM reactive, with a serological prevalence rate of 26.53%. Among all the donor samples, 26 were RT-PCR positive and/or NS1 positive. Moreover, there were significant differences in the prevalence rate of DENV in terms of occupation (P=0.001), education(P<0.001) and ethnicity (P=0.026). CONCLUSION The prevalence of DENV in Xishuangbanna Blood Center was higher than most other blood centers that have implemented DENV donor screening. Our study provides the first-hand data about the prevalence of DENV and allows development of a screening strategy for clinical use.

Detection of dengue virus serotypes by single-tube multiplex RT-PCR and multiplex real-time PCR assay

2021 ◽  
Author(s):  
Kundan Tandel ◽  
Mahadevan Kumar ◽  
G.S. Bhalla ◽  
S.P.S. Shergill ◽  
Vijaya Swarnim ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Real Time ◽  
Real Time Pcr ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Single Tube ◽  
Dengue Virus Serotypes

scholarly journals O486 Implementation of a real-time RT-PCR assay to improve diagnostics of dengue virus infections

2007 ◽  
Vol 29 ◽  
pp. S104-S105
Author(s):  
A. Dumoulin ◽  
H.P. Marti ◽  
M. Panning ◽  
H.H. Hirsch
Keyword(s):  
Dengue Virus ◽  
Real Time ◽  
Virus Infections ◽  
Rt Pcr ◽  
Pcr Assay

Detection of concurrent infection with multiple dengue virus serotypes in Thai children by ELISA and nested RT-PCR assay

2008 ◽  
Vol 153 (12) ◽  
Author(s):  
P. Chinnawirotpisan ◽  
M. P. Mammen ◽  
A. Nisalak ◽  
B. Thaisomboonsuk ◽  
S. Narupiti ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Concurrent Infection ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Dengue Virus Serotypes

scholarly journals Frequency of transovarial transmission of dengue virus in Aedes aegypti mosquitoes in endemic areas of dengue hemorrhagic fever in Ceper District, Klaten Regency, Central Java Province, Indonesia

2021 ◽  
Vol 41 ◽  
pp. 05004
Author(s):  
Novita Eka Putri ◽  
Sitti Rahmah Umniyati ◽  
Elsa Herdiana Murhandarwati
Keyword(s):  
Monoclonal Antibody ◽  
Dengue Virus ◽  
Dengue Hemorrhagic Fever ◽  
Hemorrhagic Fever ◽  
High Rate ◽  
Transovarial Transmission ◽  
Haemorrhagic Fever ◽  
Central Java ◽  
Immunocytochemical Assay ◽  
Central Java Province

Klaten Regency is an area in Central Java Province with a high rate of endemic dengue hemorrhagic fever. This study aims to demonstrate a correlation between transovarial transmission and the incidence of dengue haemorrhagic fever in Ceper District, Klaten Regency. Aedes larvae and pupae were collected from January 2019 to March 2019 from various containers, using gamadotic and dipper tools, in Ngawonggo, Kajen, and Meger villages, which have high, medium, and low numbers of dengue cases, respectively. The larvae and pupae obtained were reared into adults. The presence of dengue virus in head squashes of 1-week-old female Ae. aegypti was identified based on an immunocytochemical assay using DSSE10 monoclonal antibody, made at Universitas Gadjah Mada as a primary antibody. The frequency of transovarial transmission of dengue virus (TIR) in Ae. aegypti in the three villages was 35.7%. The highest frequency occurred in Ngawonggo Village, at 40 %, and the lowest in Meger Village, at 28%, while the frequency in Kajen Village was 36.9 %. This study proves that there is a strong correlation (r = 0.99664) between transovarial transmission and the incidence of dengue hemorrhagic fever in Ceper District, Klaten Regency, Central Java.

Clinical evaluation of an in-house-developed real-time RT-PCR assay for serotyping of dengue virus

2020 ◽  
Vol 165 (10) ◽  
pp. 2311-2315 ◽  
Author(s):  
M. B. Kakade ◽  
N. Shrivastava ◽  
J. A. Patil ◽  
D. Parashar ◽  
P. S. Shah ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Real Time ◽  
Clinical Evaluation ◽  
Rt Pcr ◽  
Pcr Assay

scholarly journals Infeksi Virus Dengue pada Nyamuk Aedes aegypti Menggunakan Artificial Blood Feeding dan Deteksi Virus Dengue Menggunakan Teknik Molekular

2019 ◽  
Vol 11 (2) ◽  
pp. 91-98
Author(s):  
Endang Srimurni Kusmintarsih ◽  
Medina Fadli Latus Syaadah ◽  
Edy Riwidiharso ◽  
R Tedjo Sasmono
Keyword(s):  
Dengue Virus ◽  
Human Blood ◽  
Blood Feeding ◽  
Feeding Time ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Fasting Period ◽  
Artificial Blood ◽  
Aedes Mosquitoes ◽  
Control Study

Abstract. Artificial blood-feeding using the parafilm-M membrane can be used as an alternative solution andsubstitute live animals as a source of blood. This method is not only be used for blood-feeding but also to infectthe dengue virus (DENV) to mosquitoes. This study was aimed to determine the effectiveness artificial bloodfeeding using parafilm-M membrane in Aedes mosquitoes originated in Indonesia and determine the positivityof mosquitoes infected by Indonesia DENV-1. DENV-1 was isolated from patient and propagated in Vero cellculture. The feeding was done in cardboard cups after mosquitos have been starved for 4-17 hours before beingfed with human blood. A conical 50ml tube was prepared, and a hole was created in the tube lid. The tubeopening was covered with parafilm. Glycerol was added into conical tube and heated in water bath for an hourat 55oC. A mixture of blood and DENV-1 was made with concentration of 10%. Detection of DENV in bloodfedmosquitos was carried out by using Simplexa Dengue Real-Time RT-PCR assay. The results showed thatthe prevalence of blood-fed mosquitoes reached 66.67% with fasting period for 17 hours. Blood feedingmosquitoes are affected by duration of fasting period, blood-feeding time, and attractants from human skinrubbed into parafilm-M membrane. The prevalence of blood-fed Ae. aegypti infected by DENV was 20.83%.This study provides information on the effectiveness of artificial parafilm membrane blood-feeding in alaboratory setting that will be useful for vector control study in Indonesia.

First Record of Natural Transovarial Transmission of Dengue Virus in Aedes albopictus from Cuba

2021 ◽  
Author(s):  
Luis Augusto Piedra ◽  
Liss Claudia Martínez ◽  
Armando Ruiz ◽  
Juan Ramón Vázquez ◽  
María Guadalupe Guzmán ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Aedes Albopictus ◽  
Nested Pcr ◽  
First Record ◽  
Transovarial Transmission ◽  
Mosquito Larvae ◽  
Rt Pcr ◽  
First Time

Transovarial transmission (TOT) of dengue virus (DENV) in Aedes spp. is an important mechanism for DENV maintenance in nature and may be important in initiating outbreaks. The objective of this study was to explore the occurrence of TOT in wild Aedes albopictus populations in Cuba. Mosquito larvae were collected in Cotorro municipality, Havana, Cuba, and identified to species. Fifteen pools of Ae. albopictus each containing 30 larvae were processed for DENV detection by using conventional RT-PCR and nested PCR. Four out of 15 pools processed were positive for DENV-3, but no other DENV serotype was detected. This is the first time TOT of DENV detected in Cuban field populations of Ae. albopictus, and this suggests that this species may be an important vector of DENV in Cuba.

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