First Record of Natural Transovarial Transmission of Dengue Virus in Aedes albopictus from Cuba

2021 ◽  
Author(s):  
Luis Augusto Piedra ◽  
Liss Claudia Martínez ◽  
Armando Ruiz ◽  
Juan Ramón Vázquez ◽  
María Guadalupe Guzmán ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Aedes Albopictus ◽  
Nested Pcr ◽  
First Record ◽  
Transovarial Transmission ◽  
Mosquito Larvae ◽  
Rt Pcr ◽  
First Time

Transovarial transmission (TOT) of dengue virus (DENV) in Aedes spp. is an important mechanism for DENV maintenance in nature and may be important in initiating outbreaks. The objective of this study was to explore the occurrence of TOT in wild Aedes albopictus populations in Cuba. Mosquito larvae were collected in Cotorro municipality, Havana, Cuba, and identified to species. Fifteen pools of Ae. albopictus each containing 30 larvae were processed for DENV detection by using conventional RT-PCR and nested PCR. Four out of 15 pools processed were positive for DENV-3, but no other DENV serotype was detected. This is the first time TOT of DENV detected in Cuban field populations of Ae. albopictus, and this suggests that this species may be an important vector of DENV in Cuba.

First record in America of Aedes albopictus naturally infected with dengue virus during the 1995 outbreak at Reynosa, Mexico

1997 ◽  
Vol 11 (4) ◽  
pp. 305-309 ◽  
Author(s):  
SERGIO IBÁÑEZ-BERNAL ◽  
BALTASAR BRISEÑO ◽  
JOHN PAUL MUTEBI ◽  
ENID ARGOT ◽  
GUADALUPE RODRÍGUEZ ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Aedes Albopictus ◽  
First Record

scholarly journals Detection of Transovarial Transmission on Aedes sp. in Gombong Kebumen Central Java

2019 ◽  
Vol 11 (2) ◽  
pp. 107-112
Author(s):  
Siwi Pramatama Mars Wijayanti ◽  
Devi Octaviana ◽  
Arnika Dwi Asti
Keyword(s):  
Dengue Virus ◽  
Endemic Area ◽  
Transovarial Transmission ◽  
Next Generation ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Descriptive Research ◽  
Central Java ◽  
Dengue Transmission ◽  
Study Sites

Abstract. Gombong Subdistrict is one of dengue-endemic area in Kebumen Regency, Central Java. The high number of dengue cases in this area raises the question of whether there has been transovarial transmission occured from the Aedes sp. to their eggs. Transovarial transmission could be dangerous because the next generation of mosquitoes can directly become competent vectors as transmitters of the dengue virus (DENV). The purpose of this study is to detect dengue virus transovarial transmission in Aedes sp. in Gombong Subdistrict. This is a descriptive research in Gombong, Semanding, and Kali Tengah villages, Gombong Sub District. A total of 300 houses, 100 houses from each village were selected in this study. There were 600 Oviposition traps (ovitraps) were installed both inside and outside of houses for 6 days. Ovitraps were calculated by its Ovitrap Index (OI). Detection of transovarial transmission was carried by rearing field mosquitoes to Filial 1 then identified by RT-PCR assay. This study showed that OI in the three villages was higher in outdoor compared to indoor positions. All tested samples were negative DENV, indicated that there were no transovarial transmission occured at the study sites. Transmission in these study areas might still through horizontal mechanism transmission by mosquito bites. Although there is no transovarial transmission, awareness of dengue transmission must be continued by eradicating of mosquito nests such as 3M plus activities on a regular basis

scholarly journals Rapid Detection and Molecular Typing of Dengue Virus by Using Multiplex-Nested-RT-PCR

2015 ◽  
Vol 11 (2) ◽  
Author(s):  
Nastiti Wijayanti ◽  
Hera Nirwati ◽  
Tri Wibawa ◽  
Aris Haryanto ◽  
S. Sutaryo
Keyword(s):  
Dengue Virus ◽  
Nested Pcr ◽  
Genotypic Variation ◽  
Clinical Samples ◽  
Rt Pcr ◽  
Virus Serotype ◽  
Pcr Multiplex ◽  
Pcr Products ◽  
One Step ◽  
Nested Pcr Assays

world. We have evaluated the combination of one-step RT-PCR and multiplex nested PCR assays for detectingdengue viruses from clinical samples. Twelve patients were screened for the dengue virus, using a pair of primersthat conserve for several Flavivirus. The results showed that in 12 suspect patients, 100% were positive for Flavivirusand there are some genotypic variation among them, that indicated by several RT-PCR products higher than 511 bp,the expected product for RT-PCR. Further assay was performed to clarify the presence and serotypes of dengue virususing multiplex nested PCR. Serotyping results indicated that 83,3% of samples can be confirmed for dengue virus.Among the dengue virus positive 16,7 % are dengue-2, 16.7 % are dengue-3, and the most common 50% are dengue-4,whereas dengue-1 were not found among the patients. The combination of RT-PCR and multiplex nested PCR assaycan be used for rapid analysis dengue samples in early phase which is potentially useful for clinical, epidemiologyand also evolutionary studies.Key words: Flavivirus, dengue virus, serotype, RT-PCR, multiplex nested PCR

scholarly journals Dengue virus serotype 4 and chikungunya virus coinfection in a traveller returning from Luanda, Angola, January 2014

2014 ◽  
Vol 19 (10) ◽  
Author(s):  
R Parreira ◽  
S Centeno-Lima ◽  
A Lopes ◽  
D Portugal-Calisto ◽  
A Constantino ◽  
...  
Keyword(s):  
Virus Infection ◽  
Dengue Virus ◽  
Chikungunya Virus ◽  
Clinical Laboratory ◽  
Phylogenetic Analyses ◽  
Genomic Sequences ◽  
Rt Pcr ◽  
Virus Serotype ◽  
First Time

A concurrent dengue virus serotype 4 and chikungunya virus infection was detected in a woman in her early 50s returning to Portugal from Luanda, Angola, in January 2014. The clinical, laboratory and molecular findings, involving phylogenetic analyses of partial viral genomic sequences amplified by RT-PCR, are described. Although the circulation of both dengue and chikungunya viruses in Angola has been previously reported, to our knowledge this is the first time coinfection with both viruses has been detected there.

Deteksi Transmisi Transovarial Virus Dengue PadaAedes AegyptiPada Wilayah Endemis Kota Pontianak

2021 ◽  
Vol 1 ◽  
pp. 984-995
Author(s):  
Malik Saepudin ◽  
Heru Subaris Kasjono ◽  
M Martini
Keyword(s):  
Polymerase Chain Reaction ◽  
Aedes Aegypti ◽  
Dengue Virus ◽  
Aedes Albopictus ◽  
Transovarial Transmission ◽  
Chain Reaction ◽  
Cross Sectional ◽  
Transmission Index ◽  
Virus Serotype ◽  
Polymerase Chain

AbstractDengue virus is highly pathogenic in humans and spreads rapidly through Aedes aegypti and Aedes albopictus mosquitoes. More than half a billion people from 100 countries in the world are at serious risk of dengue virus infection. The purpose of this study was to prove the existence of transovarial transmission of dengue virus in Aedes aegypti mosquitoes with a transovarial transmission index (TTI) in endemic areas in Pontianak, West Kalimantan. This research method is descriptive observational with a cross-sectional study. The results of the Microscopic Examination of Head Squash Preparations on the Aedes aegypti mosquito, showed the Transovarial Transmission Index in Batulayang Village was 39.60% higher, compared to Sungai Jawi Village, which was 29.30%, but both were still lower than ITT in 2012. The results of the Aedes mosquito examination aegypti using the Polymerase Chain Reaction Transcription Reaction (PCR-TR) method found the dengue virus strain. The conclusion of this study proves that the transovarial transmission of dengue virus in Aedes aegypti mosquitoes in Sungai Jawi Village is 29.30% lower than in Batulayang Village by 39.60%, and the dengue virus serotype, DENV-3, has been found.Keywords: transovarial transmission; dengue virus; Aedes aegypti AbstrakVirus Dengue sangat patogen pada manusia dan menyebar dengan cepat melalui nyamuk Aedes aegypti dan Aedes albopictus. Lebih dari setengah miliar penduduk dari 100 negara di dunia berada pada risiko serius infeksi virus dengue. Tujuan dari penelitian ini adalah untuk membuktikan adanya penularan virus dengue transovarial pada nyamuk Aedes aegypti dengan indeks transmisi transovarial (TTI) di daerah Endemis di Pontianak, Kalimantan Barat. Metode penelitian ini adalah deskriptif observasional dengan studi potong lintang. Hasil Pemeriksaan Mikroskopis Sediaan Head Squash pada nyamuk Aedes aegypti, menunjukan Indeks Transmisi Transovarial pada Kelurahan Batulayang lebih tinggi yaitu 39,60%, dibandingkan dengan Kelurahan Sungai Jawi yaitu 29,30%, tetapi keduanya masih lebih rendah dibandingkan ITT pada Tahun 2012. Hasil pemeriksaan nyamuk Aedes aegypti dengan metode Polymerase Chain Reaction Transcription Reaction(PCR-TR) ditemukan strain virus Dengue-3. Kesimpulan penelitian ini membuktikan adanya transmisi transovarial virus dengue pada nyamuk Aedes aegypti di Kelurahan Sungai Jawi sebesar 29,30% lebih rendah dibandingkan di kelurahan Batulayang sebesar 39,60%, serta berhasil ditemukan serotipe virus Dengue yaitu DENV-3.Kata Kunci : transmisi transovarial; virus dengue; Aedes aegypti

scholarly journals First record of tiger mosquito, Aedes albopictus (Diptera: Culicidae), in La Rioja: Public Health implications

2021 ◽  
Vol 43 ◽  
pp. 117-122
Author(s):  
Ignacio Ruiz-Arrondo ◽  
José Luis Cánovas ◽  
Sarah Delacour-Estrella ◽  
Paula Santibáñez ◽  
José Antonio Oteo
Keyword(s):  
Aedes Albopictus ◽  
Control Strategies ◽  
First Record ◽  
Epidemiological Surveillance ◽  
Surveillance Program ◽  
Entomological Surveillance ◽  
Invasive Mosquito ◽  
Tiger Mosquito ◽  
La Rioja ◽  
First Time

En 2016 se estableció un sistema anual de vigilancia entomológica para la detección de Aedes albopictus en la comunidad autónoma de La Rioja. En el verano de 2020, se detectó por primera vez la presencia de huevos en una zona urbana, lo que constituye el primer registro de esta especie en la región. La identificación de este mosquito invasor supone un cambio en el riesgo de aparición de casos de arbovirosis en La Rioja. La vigilancia entomológica es crucial para el diseño de estrategias de control que, junto con la vigilancia epidemiológica, puedan evitar la aparición de brotes autóctonos de arbovirosis. En este trabajo se detalla el proceso de la primera detección de mosquito tigre en La Rioja, así como las intervenciones llevadas a cabo y las perspectivas de futuro. In 2016, an annual entomological surveillance program for the detection of Aedes albopictus in the autonomous community of La Rioja was established. In the summer of 2020, the presence of eggs was detected for the first time in an urban area, which constitutes the first record of this species in the region. The identification of this invasive mosquito implies a change in the risk of occurrence of arbovirus cases in La Rioja. Entomological surveillance is crucial for the design of control strategies that, together with epidemiological surveillance, can prevent the appearance of autochthonous arbovirus outbreaks. This work is a detailed description of the first detection of tiger mosquito in La Rioja, as well as the completed interventions, and the perspectives for the future.

scholarly journals Aedes aegypti abundance, larval indices and risk for dengue virus transmission in Kinondoni district, Tanzania

2022 ◽  
Vol 50 (1) ◽  
Author(s):  
Baraka L. Ngingo ◽  
Leonard E. G. Mboera ◽  
Augustino Chengula ◽  
Ines Machelle ◽  
Mariam R. Makange ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Risk Indicators ◽  
Transmission Risk ◽  
Mosquito Larvae ◽  
Mosquito Vector ◽  
Limited Information ◽  
Rt Pcr ◽  
Vector Surveillance ◽  
Cross Sectional ◽  
Water Holding

Abstract Background Tanzania has experienced periodic dengue outbreaks with increased incidence since 2010. However, there is limited information on vector dynamics and transmission risk in most parts of the country. This study was conducted to determine Aedes mosquito abundance, larval indices and dengue virus infection rate as risk indicators for DENV transmission in Kinondoni district, Dar es Salaam, Tanzania. Methods A cross-sectional study was conducted in three wards of Kinondoni district in Tanzania between December 2019 and January 2020. In each ward, three streets were randomly selected for adult and immature mosquito sampling. The adult mosquitoes were collected using Mosquito Magnet traps, while mosquito larvae and pupae were inspected in water-holding containers in the selected household compounds. The detection of dengue virus (DENV) in female Aedes mosquitoes was done using a one-step reverse transcription–polymerase chain reaction (RT–PCR) method. Results Of the 1416 adult female mosquitoes collected, Ae. aegypti accounted for 16.8% (n = 238). A total of 333 water-holding containers were inspected and 201 (60.4%) had at least an Aedes larvae or pupae. Water-holding containers supporting the breeding of Aedes larvae and pupae included discarded car tires, flowerpots and small and large plastic containers. The overall House Index, Container Index and Breteau Index were 55.1%, 60.4% and 114.2, respectively. None of the 763 female Aedes mosquitoes tested by RT–PCR was found to be infected with DENV. Conclusion The presence and abundance Ae. aegypti mosquitoes and the large proportion of water-holding containers infested with the mosquito larvae and pupae put residents of Kinondoni district at high risk of DENV transmission. Our findings emphasize the need for continuous mosquito vector surveillance and control to prevent the possibility of future DENV outbreaks in Tanzania.

scholarly journals First two autochthonous dengue virus infections in metropolitan France, September 2010

2010 ◽  
Vol 15 (39) ◽  
Author(s):  
G La Ruche ◽  
Y Souarès ◽  
A Armengaud ◽  
F Peloux-Petiot ◽  
P Delaunay ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Vector Control ◽  
Dengue Fever ◽  
Aedes Albopictus ◽  
Case Finding ◽  
Control Measures ◽  
Active Case Finding ◽  
Virus Infections ◽  
First Time ◽  
Local Transmission

In September 2010, two cases of autochthonous dengue fever were diagnosed in metropolitan France for the first time. The cases occurring in Nice, south-east France, where Aedes albopictus is established, are evidence of dengue virus circulation in this area. This local transmission of dengue calls for further enhanced surveillance, active case finding and vector control measures to reduce the spread of the virus and the risk of an epidemic.

scholarly journals Simultaneous circulation of all four dengue serotypes in Manaus, State of Amazonas, Brazil in 2011

2012 ◽  
Vol 45 (3) ◽  
pp. 393-394 ◽  
Author(s):  
Michele de Souza Bastos ◽  
Regina Maria Pinto de Figueiredo ◽  
Rajendranath Ramasawmy ◽  
Evaulino Itapirema ◽  
João Bosco Lima Gimaque ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Rain Forest ◽  
Capital City ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Amazon Rain Forest ◽  
Dengue Outbreaks ◽  
Dengue Virus Serotypes ◽  
Polymerase Chain ◽  
First Time

INTRODUCTION: Manaus, the capital city of the state of Amazon with nearly 2 million inhabitants, is located in the middle of the Amazon rain forest and has suffered dengue outbreaks since 1998. METHODS: In this study, blood samples were investigated using reverse transcriptase-polymerase chain reaction (RT-PCR), aimed at identifying dengue virus serotypes. RESULTS: Acute phase sera from 432 patients were tested for the presence of dengue virus. Out of the 432 patients, 137 (31.3%) were found to be positive. All the four dengue virus serotypes were observed. CONCLUSIONS: The simultaneous circulation of the four dengue serotypes is described for the first time in Manaus and in Brazil.

First record of the superfamily Proctotrupoidea from French Polynesia (Hymenoptera)

2020 ◽  
Vol 125 (4) ◽  
pp. 404-406
Author(s):  
Thibault Ramage
Keyword(s):  
First Record ◽  
French Polynesia ◽  
Society Islands ◽  
First Time

A Helorid wasp, Helorus ruficornis Förster, 1856, is reported for the first time on Tahiti (Society Islands), which is also the first record of both Proctotrupoidea and family Heloridae in French Polynesia. The potential hosts of Helorus ruficornis in French Polynesia are discussed.

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