scholarly journals Potensi Minyak Atsiri Bunga Lawang (Illicium verum) sebagai Repelen Nyamuk Aedes aegypti

Author(s):  
Eva Lestari ◽  
Bondan Fajar Wahyudi ◽  
Adil Ustiawan ◽  
Dian Indra Dewi

Dengue Hemorrhagic Fever (DHF) is still a become public health problem in the world. In 2017, there were  68,407 cases with 493 deaths DHF cases in Indonesia . The incidence rate of DHF in 2017 was 26,10/100,000 population. DHF was caused by dengue virus which was transmitted through the bites of Aedes aegypti. Several types of plants such as stems, leaves, and flowers can be used as natural ingredients for repelling Ae. aegypti, Star anise (Illicium verum) has a chemical compound that can be used as a mosquito repellent. The purpose of this study was to know the potential of star anise essential oil as repellents of Ae. aegypti. This study was an experimental study with post test only with control group design. The experiment to test  the repellency of star anise essential oil was carried out using concentrations of 10%, 20%, 30%, 40%, and 50%  on 3-5 days old female Ae. aegypti. The result of this study showed that repellency index of star anise essential oil at concentration 10%, 20%, 30%, 40% and 50% during 6 hours were 58.1%; 63.51%; 59.95%; 49.45% and 65.32%. Star anise essential oil at concentration 10%, 20%, 30%, 40%, and 50% can provide protection against the bites of Ae. aegypti for 1 until 2 hours.

2021 ◽  
Vol 13 (1) ◽  
pp. 19-26
Author(s):  
Achmad Farich ◽  
Agung Aji Perdana ◽  
Dian Yunita

Dengue Haemorrhagic Fever is an infectious disease caused by the dengue virus which is transmitted mainly through the bite of the Aedes aegypti mosquito. Currently, larvicides are an important strategy in dengue vector control. Larvisides are applied to mosquito breeding sites to kill larvae. This study aims to determine the effectiveness of citronella essential oil as a larvicide for Aedes aegypti. An experimental design with a randomized post-test only control group design was applied in this study using the first to fourth instar larvae of Aedes aegypti as the test material. The concentrations of essential oils applied were 10 ppm, 50 ppm, 100 ppm, 500 ppm and control with 4 replications for 3 different days. A total of 25 Ae aegypti larvae were used in each treatment and observed for 24 hours. Data analysis was performed using univariate and bivariate (probit test and Kruskal Wallis test). The results showed that all concentrations in the treatment group had significant differences with the control group with p < 0.05. This laboratory test also revealed that citronella essential oil was effective to kill the fourth instar larvae of Aedes aegypti with an LC50 of 1.553 mg/L. Citronella essential oil was effective in killing 50% of IVth instar larvae (LT50) within 3.6 minutes. The results of the chemical composition analysis of citronella essential oil using GC-MS showed citronellal, 2,6-octadien-1-ol, 3,7-dimethyl, and citronellol as the most abundant compositions. Citronella essential oil has potential as an effective botanical pesticide to control  Aedes aegypti larvae. Abstrak Demam Berdarah Dengue adalah salah satu penyakit menular yang disebabkan virus dengue yang ditularkan dari seseorang ke orang lain melalui nyamuk Aedes aegypti. Saat ini, larvasida merupakan salah satu strategi penting dalam pengendalian vektor dengue. Tujuan dari penelitian ini adalah untuk melihat efektivitas minyak atsiri sereh wangi sebagai larvasida Aedes aegypti. Metode yang digunakan berupa eksperimental design dengan rancangan randomized post test only control group design dengan menggunakan larva Ae. aegypti instar I sampai IV sebagai material uji. Konsentrasi minyak atsiri yang digunakan yaitu 10 ppm, 50 ppm, 100 ppm, 500 ppm dan kontrol dengan  empat kali pengulangan selama tiga hari berbeda. Masing-masing perlakuan menggunakan 25 larva dan dilakukan pengamatan selama 24 jam. Analisis data dilakukan secara univariat dan bivariat (uji probit dan uji kruskal wallis). Hasil penelitian menunjukkan bahwa semua konsentrasi pada kelompok perlakuan memiliki perbedaan secara bermakna dengan kelompok kontrol p<0,05. Minyak atsiri sereh wangi pada semua konsentrasi perlakuan efektif dalam membunuh larva Ae. aegypti.  Konsentrasi minyak atsiri sereh wangi yang paling baik membunuh 50% (LC50) larva Ae. aegypti instar IV sebesar 1,553 mg/L. Minyak atsiri sereh wangi mampu membunuh larva Ae. aegypti instar IV sebanyak 50% (LT50) pada waktu 3,616 menit. Komponen utama minyak sereh atsiri sereh wangi yang teridentifikasi  dengan GC-MS adalah  citronellal, 2,6-octadien-1-ol, 3,7-dimetil, dan citronellol. Minyak atsiri sereh wangi dapat berpotensi sebagai larvasida botani yang efektif untuk mengontrol nyamuk Ae. aegypti


2017 ◽  
Vol 6 (2) ◽  
pp. 440
Author(s):  
Kharisma Putra D ◽  
Hasmiwati Hasmiwati ◽  
Arni Amir

Salah satu upaya untuk mengurangi kasus DBD adalah dengan pengendalian vektor DBD dengan larvisida. Temephos merupakan salah satu insektisida yang telah digunakan lebih dari 30 tahun dan berfungsi mengendalikan larva vektor. Penggunaan temephos yang tidak sesuai aturan dapat menyebabkan penurunan kerentanan pada vektor DBD.  Tujuan penelitian ini  adalah untuk  menilai  status  kerentanan larva Aedes aegypti di tiga kecamatan di Kota Padang. Penelitian ini menggunakan post  test  only  with  control  group design. Telur diambil dan dipelihara di laboratorium hingga mencapai larva instar III/IV. Uji kerentanan untuk temephos dilakukan berdasarkan standar WHO. Hasil penelitian menunjukkan pada Kecamatan Kuranji, kematian larva pada konsentrasi 0,005 mg/L sebesar 10%, 0,01 mg/L sebesar 45%, 0,02 mg/L sebesar 86%, dan pada konsentrasi 0,03 mg/L sebesar 100%. Pada Kecamatan Koto Tangah, kematian larva pada konsentrasi 0,005 mg/L sebesar 24%, 0,01 mg/L sebesar 48%, 0,02 mg/L sebesar 99%, dan pada konsentrasi 0,03 mg/L sebesar 100%. Pada Kecamatan Padang Timur pada konsentrasi 0,005 mg/L didapatkan kematian larva sebesar 12%, pada 0,01 mg/L sebesar 43%, pada 0,02 mg/L sebesar 99%, dan pada 0,03 mg/L sebesar 100%. Hasil uji One way-Anova adalah bermakna dengan nilai p<0,05 pada ketiga kecamatan dan LC99 sedikit diatas 0,02 mg/L. Simpulan penelitian ini adalah status kerentanan Aedes aegypti terhadap temephos di tiga kecamatan berkisar antara rentan dan toleran, belum mencapai resisten sehingga temephos masih dapat digunakan dalam pengendalian vektor DBD.


Author(s):  
Dwi Sulistia Ningrum ◽  
Siwi Pramatama Mars Wijayanti ◽  
Kuswanto Kuswanto

Aedes aegypti is the main vector which transmits Dengue virus as causing agent of Dengue Haemmorhagic Fever (DHF). Chemical control of Ae. aegypti have an impact on the environment and humans, also burden a high cost. One of the efforts to reduce the negative impact of synthetic insecticide, which is to find out alternative natural insecticide from plant-based insecticides. The purpose of this research is to determine the killing power of the rhizome bangle extract to Ae. aegypti larvae. This research was a quasi-experimental design with post test only control group design. The concentration of extract rhizome bangle used were 0%; 0.125%; 0.25%; 0.5%; 0.75% and 1%. The mosquito sample used in this study were instar III of Ae. aegypti larvae as many as 600 larvae. Data analysis used univariate and bivariate (probit and Kruskal Wallis test). The results showed that there was an effect of the concentration of extract rhizome bangle against the mortality of Ae. aegypti larvae with p=0,002 (p<0,05). Extract rhizome bangle effectively killed Ae. aegypti larvae with LC50 and LC90 were 0.148 % and 0.338 %, with the most effective concentration is 1%. Based on this research, extract rhizome bangle has a larvicidal effect on Ae. aegypti, so it can be used as an alternative method to minimize the usage of chemical larvicides that easily applied by the community.


2021 ◽  
Vol 6 (2) ◽  
pp. 76
Author(s):  
Dini Fitri Damayanti ◽  
Oon Fatonah Akbarini ◽  
Tuty Sarini

Persalinan akan memberikan pengalaman dan pengaruh jangka panjang yang sangat besar terhadap seorang perempuan dalam menjalani masa kehidupan selanjutnya. Beberapa faktor yang mempengaruhi kemajuan proses persalinan yaitu pengaturan posisi pasien, kehadiran pendamping, latihan bernafas, usapan pada punggung. Pijatan pada punggung akan menye- babkan penurunan ketegangan otot dan relaksasi termasuk pada otot abdomen dapat men- gurangi friksi antara rahim dan dinding abdomen. Aromaterapi adalah terapi yang menggu- nakan essential oil atau sari minyak murni untuk membantu menenangkan jiwa dan raga. Secara ilmiah, reaksi terjadi karena wewangian mengirimkan sinyal pada bagian otak yang mengatur emosi sehingga ibu menjadi rileks dan tenang. Kondisi relaksasi yang di alami ibu karena diberikan pijatan atau aromaterapi akan meningkatkan sirkulasi daerah genitalia serta memperbaiki elastisitas servik. Hal ini akan mempercepat pembukaan servik. Peneli- tian quasy experiment dengan pendekatan post test nonequivalent control group design den- gan non probality sampling dengan metode pemilihan sampel yaitu memilih semua individu yang bersalin di Puskesmas Sungai Kakap dan Puskesmas Sungai Durian yang memenuhi kriteria pemilihan. Hasil uji analisis Mann-Whitney menunjukkan bahwa median lama kala I fase aktif pada kelompok pijat lebih singkat 180 (110-240) menit dibanding pada kelompok yang diberikan aromaterapi 225 (120-240) menit.


2018 ◽  
pp. 10-15
Author(s):  
Alfina Baharuddin

The high number of cases of dengue fever in Indonesia, so it needs to be held vector control effort. Moringa is used as a natural anti-mosquito capable of supplying larvae. This is because there are compounds of steroids, triterpenoids, alkaloids, saponins, flavanoids, and tannins in the stem of moringa. The type of research used was experimental with post test only control group design method. The data were collected by counting the number of Aedes aegypti instar III larvae that died in each type of salinity concentration during the exposure time. The experiment was conducted 4 times, Observation, calculation and recording of the number of live and dead larvae every 24, 32, 40 and 48 hours after treatment. The data that has been collected and analyzed is then presented in tabular and narrative form. At the concentration of 3% in the Moringa leather bark estrants at the time of 24 hour measurements in each treatment of 1 (3), 2, 3, 3 (3) treatment, 4 (1) treatment with an average percentage of larva death 27 , 5%. The concentration of 3% extract of Moringa leaf bark effectively kills Aedes aegepty larvae by 45% within 48 hours. Concentration of 3.5% extract of Moringa leaf bark effectively kills Aedes aegepty larva by 45% within 24 hours. The concentration of 4% extract of Moringa leaf bark effectively kills Aedes aegepty larva by 67.5% within 24 hours. The effective exposure time of 24 hours resulted in the death of 67.5% Aedes aegypti.  


2018 ◽  
Vol 9 (2) ◽  
pp. 218
Author(s):  
Khoiriyanti Wulandari ◽  
Mei Ahyanti

<p>Dengue Hemorrhagic Fever (DHF) or Dengue Hemorrhagic Fever (DHF) is a disease caused by a virus which is very dangerous because it can cause the patient to die within a few days. Bandar Lampung city itself is a dengue endemic area. According to data from the Lampung Provincial Health Office, in 2015, from 15 there were 2,996 deaths in 31 people. The study aimed to knowing the effectiveness of bintaro seed extract (Cerbera manghas) as larvacide in III instars Aedes aegypti larvacides experimental research with post test only control group design. The sampling method was purposive sampling. Independent variables were bintaro seed extract (dose and time), the dependent variable was Aedes aegypti larvAedes The results showed that from 5 types of doses with 5 repetitions, the death result of Aedes aegypti larvae with a dose of 0% had no mortality, a dose of 0.1% with an average of 12.55, a dose of 0.5% with an average of 15.15 , 1% dose with an average of 18.20, a dose of 1.5% with an average of 22.15, and a dose of 2% with an average of 25. At the time of contact, the death result of the larvae was found with an average 6-hour contact time. an average of 8.70, 12 hours with an average of 14.13, 24 hours with an average of 18.37 and 48 hours with an average of 20.83. According to the results of statistical tests, the most effective effect of bintaro seed extract dosage is at a dose of 1.5% and contact time of 6 hours.<strong></strong></p>


2018 ◽  
Vol 9 (2) ◽  
pp. 200
Author(s):  
Amalinda Mega Novasari ◽  
Retno Sasongkowati

Dengue hemorrhagic fever is a public health problem in Indonesia that the number of DHF cases has been increased. Morbidity number of DHF per 100,000 population at 2011 was 26.67 became 34.3 in 2012. The control of DHF has been attempted, but a lot of synthetic insecticide used has been negatively affected to environment. So, it need the insecticide alternative. The aim of study was to identify the effect of solvent sugar apple (Annona squamosa L.) seeds to the death of Aedes aegypti mosquitoes by electric liquid method. Type of this research was experimental with cross sectional study design as well as the post test only control group design. The study was conducted from February to July 2013 in the Laboratory of Entomology of the Provincial Health Office of East Java. The population of this research was the Aedes aegypti with 560 sample of mosquitoes were divided into five treatments and four repetitions for each treatment. The collection of quantitative data drawn from primary data through observation by calculating the death of Aedes aegypti of each the sugar apple seeds concentration of 10%, 20%, 30%, 40% and 50% for 24 hours. The results showed that the concentration of 50% solvent of sugar apple seeds provide the greatest effect in causing the death of Aedes aegypti mosquitoes as many as 36 died after exposure. It can be concluded that there are effected by giving solvent of sugar apple (Annona squamosa L.) seeds to the death of Aedes aegypti mosquitoes by electric liquid method.


2020 ◽  
Vol 4 (2) ◽  
pp. 10
Author(s):  
I Wayan Darma Kusuma ◽  
Sang Gede Purnama

ABSTRAKNyamuk Aedes sp. adalah vektor penyakit Demam Berdarah Dengue (DBD) di Indonesia. Salah satu pengendalian vektor DBD adalah dengan melakukan fogging menggunakan insektisida. Tujuan dari penelitian ini adalah untuk mengetahui tingkat kerentanan nyamuk Aedes sp. terhadap fogging insektisida melathion 5% di wilayah kota Denpasar sebagai daerah endemis DBD. Penelitian ini merupakan jenis penelitian eksperimental murni dengan rancangan penelitian post-test only with control group design. Jumlah sampel yang digunakan dalam penelitian ini adalah 200 ekor nyamuk dewasa yang dibagi ke dalam kerangka besi masing-masing 25 ekor. Hasil dari penelitian menunjukkan bahwa nyamuk Aedes sp. masih rentan terhadap insektisida melathion dengan konsentrasi 5% dengan kematian 100% dalam waktu 60 menit di dalam maupun di luar ruangan rumah. Berdasarkan hasil tersebut dapat disimpulkan bahwa daya bunuh fogging insektisida melathion 5% terhadap nyamuk Aedes sp. di wilayah Kota Denpasar secara umum masih efektif dan tidak terdapat perbedaan daya bunuh fogging insektisida melathion 5% terhadap nyamuk Aedes sp. di dalam rumah dengan di luar rumah.Keywords: Aedes sp, Melathion, Resistensi. ABSTRACTAedes Sp. Is a vector of Dengue Hemorrhagic Fever (DHF) in Indonesia. Among many ways to control DHF, fogging technique using Insecticides is commonly used. The purpose is this study was to determine susceptibility of the Aedes sp. against fogging of 5% malathion insecticide in the Denpasar city area as a DHF endemic area. This research is a pure experimental research with post-test only with control group design. The number of samples used in this study were 200 adult mosquitoes which were divided into an iron frame of 25 individuals each. The study showed that the Aedes sp. still susceptible to malathion insecticide with a concentration of 5% with 100% death within 60 minutes inside and outside the home. Based on the study it can be concluded that fogging technique using 5% Malathion is effective to kill Aedes Sp. The study also found that there is no difference result of killing power by applying 5% Malathion inside and outside the house.Keywords: Aedes Sp., Malathion, Resistance


Biomedika ◽  
2015 ◽  
Vol 7 (2) ◽  
Author(s):  
Reisya Tiara Kandita ◽  
Riandini Aisyah ◽  
Wulandari Berliani Putri

Nyamuk Aedes aegypti dan Anopheles aconitus merupakan beberapa vektor penting pada penyakit tropis di Indonesia, antara lain seperti penyakit demam berdarah dan malaria. Salah satu cara pemberantasan nyamuk yang paling sering digunakan adalah dengan menggunakan insektisida. Penggunaan insektisida kimiawi yang bertujuan untuk membunuh nyamuk dewasa juga menimbulkan permasalahan tersendiri yaitu timbulnya resistensi nyamuk dan efek toksik pada manusia. Oleh karena itu, diperlukan insektisida alternatif yang lebih aman terhadap lingkungan yang berasal dari tanaman. Salah satunya dengan menggunakan ekstrak buah Leunca (Solanum nigrum). Buah Leunca mengandung glikoalkaloid yang beracun. Penelitian ini bertujuan untuk membuktikan ekstrak buah Leunca mempunyai potensi sebagai insektisida alami terhadap nyamuk Aedes aegypti dan Anopheles aconitus. Penelitian ini merupakan penelitian eksperimental laboratoris dengan rancangan true experimental-post test only control group design. Sampel yang digunakan adalah nyamuk Aedes aegypti dengan konsentrasi ekstrak 20%, 40%, 60%, dan 80% dan nyamuk Anopheles aconitus dengan konsentrasi ekstrak 5%, 10%, 20% dan 40%. Dilakukan penyemprotan pada glass chamber kemudian diamati sampai 20 menit, setelah itu nyamuk dipindah ke dalam paper cup dan diamati selama 24 jam. Pengulangan dilakukan sebanyak empat kali. Uji yang digunakan adalah uji Oneway Anova dengan nilai p < 0,05 dan analisis probit.Hasil uji Oneway Anova diperoleh nilai signifikansi < 0,05. Hasil penelitian menunjukkan bahwa ekstrak buah Leunca memiliki efek insektisida terhadap nyamuk Aedes aegypti dan Anopheles aconitus. Hasil analisis probit nyamuk Aedes aegypti LC50 terletak antara 69,890% dan 156,417% dengan estimasi 91,128% dan LC90 terletak antara 337,934% dan 5842,532% dengan estimasi 785,398%, sedangkan nyamuk Anopheles aconitus LC50 terletak antara 19,809% dan 33,570%. Dengan estimasi 24,767% dan LC90 terletak antara 96,540% dengan 450,875% dengan estimasi 169,046%. Peneliti menyimpulkan bahwa ekstrak buah Leunca (Solanum nigrum L.) memiliki efek insektisida terhadap nyamuk Aedes aegypti dengan konsentrasi 80% dan Anopheles aconitus dengan konsentrasi 40%.Kata Kunci: Ekstrak buah Leunca (Solanum nigrum), insektisida, Aedes aegypti, Anopheles aconitus


2017 ◽  
Vol 4 (3) ◽  
pp. 318
Author(s):  
Angger Luhung Nur Fadilah ◽  
Widya Hary Cahyati ◽  
Rudatin Windraswara

Demam Berdarah Dengue (DBD) ditularkan melalui gigitan nyamuk Aedes betina. Salah satu upaya pengendalian vektor yang populer di masyarakat yaitu menggunakan insektisida kimiawi akan tetapi menyebabkan resistensi pada nyamuk dan keracunan pada manusia. Upaya pengendalian dapat berupa insektisida alami yang terbuat dari tumbuhan, salah satunya yaitu daun pepaya (Carica papaya L). Tujuan dari penelitian ini adalah untuk mengetahui daya proteksi lotion ekstrak daun pepaya untuk menolak Aedes aegypti. Jenis penelitian ini adalah eskperimen murni dengan rancangan penelitian post test only control group design. Populasi penelitian adalah telur nyamuk Aedes aegypti yang dikembangbiakkan menjadi nyamuk, sampel 50 ekor nyamuk Aedes aegypti umur 2-5 hari untuk setiap kelompok perlakuan dengan 4 kali pengulangan, sehingga total nyamuk yang dibutuhkan 1.400 nyamuk Aedes aegypti karena jumlah perlakuan 7 kelompok. Data dianalisis menggunakan uji Anova dan Post Hoc. Hasil penelitian ini menunjukkan terdapat perbedaan persentase jumlah nyamuk yang hinggap pada berbagai konsentrasi lotion ekstrak daun pepaya dengan nilai signifikansi p=0,001 (p


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