scholarly journals Efektivitas Ekstrak Biji Bintaro (Cerbera manghas) sebagai Larvasida Hayati pada Larva Aedes aegypti Instar III

2018 ◽  
Vol 9 (2) ◽  
pp. 218
Author(s):  
Khoiriyanti Wulandari ◽  
Mei Ahyanti

<p>Dengue Hemorrhagic Fever (DHF) or Dengue Hemorrhagic Fever (DHF) is a disease caused by a virus which is very dangerous because it can cause the patient to die within a few days. Bandar Lampung city itself is a dengue endemic area. According to data from the Lampung Provincial Health Office, in 2015, from 15 there were 2,996 deaths in 31 people. The study aimed to knowing the effectiveness of bintaro seed extract (Cerbera manghas) as larvacide in III instars Aedes aegypti larvacides experimental research with post test only control group design. The sampling method was purposive sampling. Independent variables were bintaro seed extract (dose and time), the dependent variable was Aedes aegypti larvAedes The results showed that from 5 types of doses with 5 repetitions, the death result of Aedes aegypti larvae with a dose of 0% had no mortality, a dose of 0.1% with an average of 12.55, a dose of 0.5% with an average of 15.15 , 1% dose with an average of 18.20, a dose of 1.5% with an average of 22.15, and a dose of 2% with an average of 25. At the time of contact, the death result of the larvae was found with an average 6-hour contact time. an average of 8.70, 12 hours with an average of 14.13, 24 hours with an average of 18.37 and 48 hours with an average of 20.83. According to the results of statistical tests, the most effective effect of bintaro seed extract dosage is at a dose of 1.5% and contact time of 6 hours.<strong></strong></p>

2020 ◽  
Vol 4 (2) ◽  
pp. 10
Author(s):  
I Wayan Darma Kusuma ◽  
Sang Gede Purnama

ABSTRAKNyamuk Aedes sp. adalah vektor penyakit Demam Berdarah Dengue (DBD) di Indonesia. Salah satu pengendalian vektor DBD adalah dengan melakukan fogging menggunakan insektisida. Tujuan dari penelitian ini adalah untuk mengetahui tingkat kerentanan nyamuk Aedes sp. terhadap fogging insektisida melathion 5% di wilayah kota Denpasar sebagai daerah endemis DBD. Penelitian ini merupakan jenis penelitian eksperimental murni dengan rancangan penelitian post-test only with control group design. Jumlah sampel yang digunakan dalam penelitian ini adalah 200 ekor nyamuk dewasa yang dibagi ke dalam kerangka besi masing-masing 25 ekor. Hasil dari penelitian menunjukkan bahwa nyamuk Aedes sp. masih rentan terhadap insektisida melathion dengan konsentrasi 5% dengan kematian 100% dalam waktu 60 menit di dalam maupun di luar ruangan rumah. Berdasarkan hasil tersebut dapat disimpulkan bahwa daya bunuh fogging insektisida melathion 5% terhadap nyamuk Aedes sp. di wilayah Kota Denpasar secara umum masih efektif dan tidak terdapat perbedaan daya bunuh fogging insektisida melathion 5% terhadap nyamuk Aedes sp. di dalam rumah dengan di luar rumah.Keywords: Aedes sp, Melathion, Resistensi. ABSTRACTAedes Sp. Is a vector of Dengue Hemorrhagic Fever (DHF) in Indonesia. Among many ways to control DHF, fogging technique using Insecticides is commonly used. The purpose is this study was to determine susceptibility of the Aedes sp. against fogging of 5% malathion insecticide in the Denpasar city area as a DHF endemic area. This research is a pure experimental research with post-test only with control group design. The number of samples used in this study were 200 adult mosquitoes which were divided into an iron frame of 25 individuals each. The study showed that the Aedes sp. still susceptible to malathion insecticide with a concentration of 5% with 100% death within 60 minutes inside and outside the home. Based on the study it can be concluded that fogging technique using 5% Malathion is effective to kill Aedes Sp. The study also found that there is no difference result of killing power by applying 5% Malathion inside and outside the house.Keywords: Aedes Sp., Malathion, Resistance


Author(s):  
Eva Lestari ◽  
Bondan Fajar Wahyudi ◽  
Adil Ustiawan ◽  
Dian Indra Dewi

Dengue Hemorrhagic Fever (DHF) is still a become public health problem in the world. In 2017, there were  68,407 cases with 493 deaths DHF cases in Indonesia . The incidence rate of DHF in 2017 was 26,10/100,000 population. DHF was caused by dengue virus which was transmitted through the bites of Aedes aegypti. Several types of plants such as stems, leaves, and flowers can be used as natural ingredients for repelling Ae. aegypti, Star anise (Illicium verum) has a chemical compound that can be used as a mosquito repellent. The purpose of this study was to know the potential of star anise essential oil as repellents of Ae. aegypti. This study was an experimental study with post test only with control group design. The experiment to test  the repellency of star anise essential oil was carried out using concentrations of 10%, 20%, 30%, 40%, and 50%  on 3-5 days old female Ae. aegypti. The result of this study showed that repellency index of star anise essential oil at concentration 10%, 20%, 30%, 40% and 50% during 6 hours were 58.1%; 63.51%; 59.95%; 49.45% and 65.32%. Star anise essential oil at concentration 10%, 20%, 30%, 40%, and 50% can provide protection against the bites of Ae. aegypti for 1 until 2 hours.


2021 ◽  
Vol 913 (1) ◽  
pp. 012110
Author(s):  
N F Dhenge ◽  
P Pakan ◽  
K Lidia

Abstract Dengue Hemorrhagic Fever (DHF) is an infection caused by the dengue virus, which is still a public health problem in Indonesia. Chemical insecticides as larvicides used to control Aedes aegypti have created resistant populations. Thus, higher doses are needed, which, of course, have toxic effects on animals, humans, and the environment.To analyze the effectiveness of papaya leaf extract (Carica papaya) against the mortality of the third instar Aedesaegypti mosquito larvae. This study is a true experimental research design with a posttest-only control group design. In this study, the groups in this study were the larvae of the Aedes aegypti mosquito instar III/IV, papaya leaf ethanol extract, abate as a positive control group, and aquadest as a negative control.The results of the mean number of larvae deaths in each treatment group was zero (0) larvae in negative control group, positive control group (25 larvae), 5% concentration (9.5 larvae), 10% concentration (11.75 larvae), 15% concentration (12.75 larvae), a concentration of 20% (14.75 larvae), and a concentration of 25% (19.5 larvae). The results of Kruskal Wallis analysis showed that papaya leaf extract was effective as a larvicide for Aedesaegypti (p=0.001). In the probit analysis, it was found that the LC50 of the extract against Aedes aegypti was 23%, while the LC99 was 55%.Papaya leaf extract is effective as a vegetable larvicide for 3rd/IV instar Aedesaegypti larvae


2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Wawan Kurniawan

ABSTRAK  Latar belakang: Demam Berdarah Dengue (DBD) disebabkan oleh virus Dengue dapat menyebabkan kematian. Pencegahan DBD yang dianggap paling tepat adalah Pemberantasan Sarang Nyamuk (PSN). Penelitian ini bertujuan untuk mengetahui pengaruh pelatihan pengendalian vektor Demam Berdarah pada siswa sekolah dasar terhadap Maya Index di Majalengka. Metode: Penelitian ini menggunakan desain quasi experiment (pretest-posttest control group design). Sebanyak 4 sekolah terpilih sebagai kelompok intervensi dan 4 sekolah lainnya sebagai kontrol. Subyek penelitian adalah siswa kelas IV-VI yang terdiri dari 171 siswa pada kelompok intervensi dan 163 pada kelompok kontrol. Instrumen yang digunakan adalah formulir pemantauan jentik berkala. Hasil: Jumlah rumah dengan kategori Maya Index tinggi berkurang dari 27,5% menjadi 9,4%. Terjadi penurunan angka BRI kategori tinggi pada kelompok intervensi dari 20,5% menjadi 1,8%. Pada kelompok kontrol tidak terjadi penurunan angka BRI kategori tinggi (22,1%), sebaliknya terjadi penurunan kategori rendah dari 34,4% menjadi 3,7%. Tidak terjadi penurunan angka HRI pada kelompok intervensi maupun kontrol. Kesimpulan: Pelatihan pengendalian vektor Demam Berdarah dapat menurunkan nilai BRI dan Maya Index, tetapi tidak berpengaruh terhadap nilai HRI. Tidak adanya perubahan nilai HRI menunjukkan bahwa kebersihan dan sanitasi lingkungan merupakan faktor yang berpengaruh terhadap probabilitas kejadian demam berdarah. Kata Kunci : Demam Berdarah, Maya Index, pelatihan, pengendalian vektor   ABSTRACT Background: Dengue Hemorrhagic Fever (DHF) caused by Dengue virus could cause death. The most appropriate prevention of Dengue is eradication of mosquito nests (PSN). This study aims to determine the effect of Dengue vector control training on elementary students towards Maya Index in Majalengka. Method: This study used quasi-experimental design (pretest-posttest control group design). A total of 4 schools were selected as intervention groups and 4 other schools as controls. The subjects were students in grades IV-VI consisting of 171 students in the intervention group and 163 in the control group. The instrument used was periodic larva monitoring form. Results: The number of houses with a high Maya Index category in the intervention group decreased from 27.5% to 9.4%. There was a decrease in the high BRI category in the intervention group from 20.5% to 1.8%. In the control group, there was no decrease in the high BRI category (22.1%), on the contrary, there was a decrease in the low category from 34.4% to 3.7%. There was no decrease in HRI rates both of intervention or control groups. Conclusion: Dengue Fever vector control training could decrease the value of BRI and Maya Index, but does not affect the value of HRI. The absence of changes in HRI  indicate that environmental hygiene and sanitation are factors that influence the probability of dengue fever occurrence. Keywords: Dengue Hemorrhagic Fever, Maya Index, training, vector control


2017 ◽  
Vol 6 (2) ◽  
pp. 440
Author(s):  
Kharisma Putra D ◽  
Hasmiwati Hasmiwati ◽  
Arni Amir

Salah satu upaya untuk mengurangi kasus DBD adalah dengan pengendalian vektor DBD dengan larvisida. Temephos merupakan salah satu insektisida yang telah digunakan lebih dari 30 tahun dan berfungsi mengendalikan larva vektor. Penggunaan temephos yang tidak sesuai aturan dapat menyebabkan penurunan kerentanan pada vektor DBD.  Tujuan penelitian ini  adalah untuk  menilai  status  kerentanan larva Aedes aegypti di tiga kecamatan di Kota Padang. Penelitian ini menggunakan post  test  only  with  control  group design. Telur diambil dan dipelihara di laboratorium hingga mencapai larva instar III/IV. Uji kerentanan untuk temephos dilakukan berdasarkan standar WHO. Hasil penelitian menunjukkan pada Kecamatan Kuranji, kematian larva pada konsentrasi 0,005 mg/L sebesar 10%, 0,01 mg/L sebesar 45%, 0,02 mg/L sebesar 86%, dan pada konsentrasi 0,03 mg/L sebesar 100%. Pada Kecamatan Koto Tangah, kematian larva pada konsentrasi 0,005 mg/L sebesar 24%, 0,01 mg/L sebesar 48%, 0,02 mg/L sebesar 99%, dan pada konsentrasi 0,03 mg/L sebesar 100%. Pada Kecamatan Padang Timur pada konsentrasi 0,005 mg/L didapatkan kematian larva sebesar 12%, pada 0,01 mg/L sebesar 43%, pada 0,02 mg/L sebesar 99%, dan pada 0,03 mg/L sebesar 100%. Hasil uji One way-Anova adalah bermakna dengan nilai p<0,05 pada ketiga kecamatan dan LC99 sedikit diatas 0,02 mg/L. Simpulan penelitian ini adalah status kerentanan Aedes aegypti terhadap temephos di tiga kecamatan berkisar antara rentan dan toleran, belum mencapai resisten sehingga temephos masih dapat digunakan dalam pengendalian vektor DBD.


Author(s):  
Dwi Sulistia Ningrum ◽  
Siwi Pramatama Mars Wijayanti ◽  
Kuswanto Kuswanto

Aedes aegypti is the main vector which transmits Dengue virus as causing agent of Dengue Haemmorhagic Fever (DHF). Chemical control of Ae. aegypti have an impact on the environment and humans, also burden a high cost. One of the efforts to reduce the negative impact of synthetic insecticide, which is to find out alternative natural insecticide from plant-based insecticides. The purpose of this research is to determine the killing power of the rhizome bangle extract to Ae. aegypti larvae. This research was a quasi-experimental design with post test only control group design. The concentration of extract rhizome bangle used were 0%; 0.125%; 0.25%; 0.5%; 0.75% and 1%. The mosquito sample used in this study were instar III of Ae. aegypti larvae as many as 600 larvae. Data analysis used univariate and bivariate (probit and Kruskal Wallis test). The results showed that there was an effect of the concentration of extract rhizome bangle against the mortality of Ae. aegypti larvae with p=0,002 (p<0,05). Extract rhizome bangle effectively killed Ae. aegypti larvae with LC50 and LC90 were 0.148 % and 0.338 %, with the most effective concentration is 1%. Based on this research, extract rhizome bangle has a larvicidal effect on Ae. aegypti, so it can be used as an alternative method to minimize the usage of chemical larvicides that easily applied by the community.


2018 ◽  
pp. 10-15
Author(s):  
Alfina Baharuddin

The high number of cases of dengue fever in Indonesia, so it needs to be held vector control effort. Moringa is used as a natural anti-mosquito capable of supplying larvae. This is because there are compounds of steroids, triterpenoids, alkaloids, saponins, flavanoids, and tannins in the stem of moringa. The type of research used was experimental with post test only control group design method. The data were collected by counting the number of Aedes aegypti instar III larvae that died in each type of salinity concentration during the exposure time. The experiment was conducted 4 times, Observation, calculation and recording of the number of live and dead larvae every 24, 32, 40 and 48 hours after treatment. The data that has been collected and analyzed is then presented in tabular and narrative form. At the concentration of 3% in the Moringa leather bark estrants at the time of 24 hour measurements in each treatment of 1 (3), 2, 3, 3 (3) treatment, 4 (1) treatment with an average percentage of larva death 27 , 5%. The concentration of 3% extract of Moringa leaf bark effectively kills Aedes aegepty larvae by 45% within 48 hours. Concentration of 3.5% extract of Moringa leaf bark effectively kills Aedes aegepty larva by 45% within 24 hours. The concentration of 4% extract of Moringa leaf bark effectively kills Aedes aegepty larva by 67.5% within 24 hours. The effective exposure time of 24 hours resulted in the death of 67.5% Aedes aegypti.  


2017 ◽  
Vol 3 (1) ◽  
pp. 55
Author(s):  
Finta Isti Kundarti ◽  
Ira Titisari ◽  
Naning Tri Windarti

Pain in the labor are predisposition for anxiety, hyperventilation, thus causing oxygen requirements and increased blood pressure. Pain in the labor  can be reduced by non- pharmacological methods, one of which is using aromatherapy. Lavender as an aromatherapy effect relaxation, pain relief, reduces anxiety and causes calmness. The purpose of this research was to know the effect of lavender massage aromatherapy to the level of the first stage of labor pain in the active phase of first stage labor. The research design used quasi experiment with pre-test post-test with control group design. The population in this research that the whole maternal active phase of the first stage in BPM Blabak’s Community Health Center Kediri Regency. The research time is June 23 until July 19 sampling techniques. The research instrument used scale observation. Statistical tests in this study using the paired t test.The results showed t value (11,000) ; t table (2,262) and P value (0.000) ; α (0.05), then H0 is rejected, which means there is effect of lavender (Lavandula angustifolia) massage aromatherapy to the pain labor level of the active phase in the first stage of labor. So the conclusion of lavender massage aromatherapy down the level of pain  in the active phase of the first stage of labor. This method can be applied midwives to help reduce pain during childbirth.; Keywords: Lavender Aromatherapy, Pain, Labor, Massage


2018 ◽  
Vol 9 (2) ◽  
pp. 200
Author(s):  
Amalinda Mega Novasari ◽  
Retno Sasongkowati

Dengue hemorrhagic fever is a public health problem in Indonesia that the number of DHF cases has been increased. Morbidity number of DHF per 100,000 population at 2011 was 26.67 became 34.3 in 2012. The control of DHF has been attempted, but a lot of synthetic insecticide used has been negatively affected to environment. So, it need the insecticide alternative. The aim of study was to identify the effect of solvent sugar apple (Annona squamosa L.) seeds to the death of Aedes aegypti mosquitoes by electric liquid method. Type of this research was experimental with cross sectional study design as well as the post test only control group design. The study was conducted from February to July 2013 in the Laboratory of Entomology of the Provincial Health Office of East Java. The population of this research was the Aedes aegypti with 560 sample of mosquitoes were divided into five treatments and four repetitions for each treatment. The collection of quantitative data drawn from primary data through observation by calculating the death of Aedes aegypti of each the sugar apple seeds concentration of 10%, 20%, 30%, 40% and 50% for 24 hours. The results showed that the concentration of 50% solvent of sugar apple seeds provide the greatest effect in causing the death of Aedes aegypti mosquitoes as many as 36 died after exposure. It can be concluded that there are effected by giving solvent of sugar apple (Annona squamosa L.) seeds to the death of Aedes aegypti mosquitoes by electric liquid method.


Sains Medika ◽  
2017 ◽  
Vol 8 (1) ◽  
pp. 15
Author(s):  
Sri Mastuti ◽  
Djoko Tri Hadi ◽  
Chodidjah Chodidjah

LATAR BELAKANG: Luka tidak sembuh dalam waktu lama dengan berbagai penyebab merupakan masalah yang sering ditemukan dalam berbagai disiplin kedokteran. Kejadian ini salah satu sumber utama morbiditas, penyebab gangguan psikologis para penderita, meningkatkan biaya pengobatan dan kehilangan jam kerja pada penderita usia produktif.�TUJUAN: untuk mengetahui pengaruh ekstrak biji jinten hitam (Nigella sativa) dalam sediaan salep sebanyak 40% dan 60% terhadap jumlah fibroblas dan kolagen pada penyembuhan luka sayat mencit balb/c.METODE: Penelitian eksperimental dengan rancangan �post test only control group design� pada 48 ekor mencit jantan galur balb/c, dibagi menjadi 4 kelompok pengujian fibroblast (24 ekor) dan 4 kelompok pengujian kolagen (24 ekor). Masing-masing kelompok terdiri dari 6 ekor sampel yang dipilih secara random. Salep ekstrak Nigella sativa dengan konsentrasi 40% dan dengan konsentrasi 60% dioles sebanyak 3x sehari selama 7 hari pada kelompok fibroblast dan selama 14 hari pada kelompok kolagen. Data dianalisis dengan Kruskal-Wallis.HASIL: Analisis jumlah fibroblast antara kelompok kontrol/tidak diberikan perlakuan dan kelompok yang diberikan salep garamycin terdapat perbedaan (p value =0,010), antara kelompok kontrol dan kelompok yang diberikan salep ekstrak jinten hitam 40 % tidak ada perbedaan (p value =0,065), pada kelompok yang diberikan salep ekstrak jinten hitam 60 % terdapat perbedaan (p value =0,025). Hasil analisis jumlah kolagen menunjukkan perbedaan antara semua kelompok (p value =0,004).KESIMPULAN: Pemberian salep ekstrak biji jinten hitam (Nigella sativa) dapat meningkatkan jumlah fibroblas dan kolagen pada penyembuhan� luka sayat kulit mencit galur balb/c.


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