scholarly journals A new and ecological miniaturized method by spectrophotometry for quantification of vancomycin in dosage form

2021 ◽  
Vol 5 (1) ◽  
pp. 39-45
Author(s):  
Patrícia Nascimento ◽  
Ana Kogawa ◽  
Hérida Regina Nunes Salgado
Keyword(s):  
Low Cost ◽  
Test Sample ◽  
Routine Analysis ◽  
Ultraviolet Region ◽  
Forced Degradation ◽  
Buffer Solution ◽  
Solution Ph ◽  
Filtration Step ◽  
The Stability ◽  
Aqueous Conditions

Vancomycin, an important antibiotic, is marketed as lyophilized powder. In the context of routine analysis of this product, the existence of a more advantageous and effective method is interesting. Thus, the objective of this work is to develop and validate a new analytical method, faster, low-cost, ecological and miniaturized for quantification of vancomycin in lyophilized powder using spectrophotometry in ultraviolet region. Buffer solution pH 6.8, quartz cuvette with capacity of 700 µL and 280 nm were chosen. The method proved to be linear in the range of 50-150 µg/mL (0.9997). The selectivity of the method was proven in two ways: The standard-sample overlay aimed to identify vancomycin in the sample; The forced degradation test (sample solutions prepared in 0.01 M HCl, 0.01 M NaOH and aqueous conditions and kept at 60 ºC by 8 hours, and UV 254 nm at ambient temperature during 24 hours) aimed to show the susceptibility of the method to consequently indicate the stability of the sample. It was precise in intraday (RSD 1.27%), interday (RSD 1.18%) and between analysts (RSD 1.92%) levels. It was robust when small variations were performed in seven important parameters (wavelength, cuvette, filtration step, dibasic and monobasic phosphate brand, ultrasound time and source of water). The accuracy was proved by the standard recovery test and showed mean recovery of 101.10%. This method can be applied in routine analysis of quality control of vancomycin lyophilized powder and it is an effective, accessible and ecological alternative, which follows the Green Analytical Chemistry principles, presenting less waste generation, no use of toxic solvents, smaller sample volumes and required diluents, which impacts on the final cost of the analyzes.

Development and Validation of Stability Indicating HPLC Method for the Determination of Impurities in the Sterile Mixture of Cefoperazone and Sulbactam

2019 ◽  
Vol 15 (7) ◽  
pp. 762-775
Author(s):  
Ramu Ivaturi ◽  
Thuttagunta Manikya Sastry ◽  
Satyaveni Sunkara
Keyword(s):  
Quantitative Estimation ◽  
Ammonium Hydroxide ◽  
Hplc Method ◽  
Forced Degradation ◽  
Buffer Solution ◽  
Solution Ph ◽  
Pharmaceutical Dosage Forms ◽  
Stability Indicating ◽  
Validation Parameters ◽  
Abdominal Infections

Background: Cefoperazone Sulbactam injection is a cephalosporin antibiotic with a β- lactamase inhibitor used in the treatment for intra abdominal infections, Urinary track infections, surgical infections, etc. The combination is not official in any of the pharmacopeia for their content and impurities determination. Introduction: The present study involves the development of a simple, rapid, accurate, sensitive and stability indicating RP-HPLC method for the quantitative estimation of Cefoperazone Sulbactam mixture and its impurities in bulk and pharmaceutical dosage forms. Methods: 0.005 M Tetrabutyl ammonium hydroxide buffer solution pH adjusted to 6.80 and Acetonitrile combination has been used in a gradient programme with a flow rate of 1.0 ml/min. The retention time of Cefoperazone and Sulbactam were observed at around 8.5 and 19.5 minutes respectively. The UV detection was carried out at a wavelength of 230 nm. The chromatographic separation was achieved using Waters xbridge C18-150*4.6 mm, 3.5 µm HPLC column. The method has been validated according to the current International Council for Harmonization (ICH) guidelines for the method validation parameters such as Specificity, linearity, range, accuracy, precision, robustness and sensitivity. Results: The validation results indicate that the method is specific, as the known impurities and other impurities formed during the forced degradation studies were not co-eluting with the main components. Moreover, all these impurities were found to be spectrally pure, proving the stability indicating power of the method. The linearity and range of the method is in the range of 0.01-150%, highly accurate (100.2%), precise (<1%) and robust. Conclusion: The proposed method was accurate and specific for the quantitative analysis of Cefoperazone and Sulbactam and their related impurities in the sterile mixture. Hence the proposed method can be used for the quantification of impurities in routine as well as stability analysis in the development as well as quality control laboratories.

Non-enzymatic determination of purine nucleotides using a carbon dot modified glassy carbon electrode

2019 ◽  
Vol 11 (30) ◽  
pp. 3866-3873 ◽  
Author(s):  
R. Karthikeyan ◽  
D. James Nelson ◽  
S. Abraham John
Keyword(s):  
Glassy Carbon ◽  
Low Cost ◽  
Phosphate Buffer Solution ◽  
Purine Nucleotides ◽  
Buffer Solution ◽  
Solution Ph ◽  
Carbon Dot ◽  
Enzymatic Determination ◽  
Sensitive Determination

Selective and sensitive determination of one of the purine nucleotides, inosine (INO) using a low cost carbon dot (CD) modified glassy carbon (GC) electrode in 0.2 M phosphate buffer solution (pH 7.2) was demonstrated in this paper.

scholarly journals New Spectrophotometric Method for Determination of Cadmium

2009 ◽  
Vol 6 (s1) ◽  
pp. S496-S500
Author(s):  
K. S. Parikh ◽  
R. M. Patel ◽  
K. N. Patel
Keyword(s):  
Stability Constant ◽  
Spectrophotometric Method ◽  
Basic Medium ◽  
Buffer Solution ◽  
Solution Ph ◽  
Yellow Colour ◽  
Maximum Absorbance ◽  
The Stability

The reagent 2-hydroxy-4-n-butoxy-5-bromopropiophenone thiosemicarbazone (HBBrPT) has been used for the determination of Cd(II) by using spectrophotometric method. The reagent HBBrPT gave an intense yellow colour with Cd(II) solution in basic medium. The maximum absorbance was observed at 440 nm, in basic buffer solution (pH 10.00). The molor absorptivity and Sandell’s sensitivity of Cd(II)-HBBrPT complex were 4035 mol-1cm-1and 0.02765 μg cm-2respectively. The stability constant of 1:2 Cd(II)-HBBrPT complex was 8.46×106. The effect of various iron was also studied.

scholarly journals Color Stability of Phycoerythrin Crude Extract (PECE) from Rhodomonas Salina Toward Physicochemical Factors

2019 ◽  
Vol 14 (1) ◽  
pp. 21
Author(s):  
Endar Marraskuranto ◽  
Tri Joko Raharjo ◽  
Rina Sri Kasiamdari ◽  
Tri Rini Nuringtyas
Keyword(s):  
Phosphate Buffer Solution ◽  
Color Stability ◽  
Ethanol Solution ◽  
Future Application ◽  
Buffer Solution ◽  
Solution Ph ◽  
Rhodomonas Salina ◽  
Freeze Dried ◽  
Physicochemical Factors ◽  
The Stability

Rhodomonas salina produces Cr-phycoerythrin545 as its designated phycoerythrin (PE) with an absorption maximum at 545 nm and a shoulder 564 nm. PE has potential to be applied as colorants, pharmaceutical agents, and fluorescent dye tags. The stability of the PE color is influenced by the physicochemical factors of the solution. This study aimed to analyze the color stability of PECE against chemical (ethanol and pH) and physical (light and temperature) factors. PECE was prepared from freeze-dried biomass of R. salina and was extracted in phosphate buffer solution (pH = 6.0) using a freeze-thaw method in -25 oC (2 hours) and 4 oC (24 hours). The resulting extract was concentrated and dried in a freeze-dryer. Analyses were conducted using UV-visible and fluorescence spectrophotometer. PECE showed color stability against light of white fluorescent lamp exposure up to 8 hours, temperature exposure up to 40 oC, ethanol solution up to concentration of 20 % (v/v), and pH range 3.9-8.42. Results from this study can be useful for extraction, purification, and future application of Cr-PE545.

scholarly journals A Graphene-Based Enzymatic Biosensor Using a Common-Gate Field-Effect Transistor for L-Lactic Acid Detection in Blood Plasma Samples

Sensors ◽  
2021 ◽  
Vol 21 (5) ◽  
pp. 1852
Author(s):  
Ariadna Schuck ◽  
Hyo Eun Kim ◽  
Júlia Konzen Moreira ◽  
Priscila Schmidt Lora ◽  
Yong-Sang Kim
Keyword(s):  
Lactic Acid ◽  
Point Of Care ◽  
Low Cost ◽  
Lactic Acid Production ◽  
Active Surface ◽  
The Body ◽  
Human Organism ◽  
Buffer Solution ◽  
Common Gate ◽  
The Stability

Lactate is an important organic molecule that is produced in excess during anaerobic metabolism when oxygen is absent in the human organism. The concentration of this substance in the body can be related to several medical conditions, such as hemorrhage, respiratory failure, and ischemia. Herein, we describe a graphene-based lactate biosensor to detect the concentrations of L-lactic acid in different fluids (buffer solution and plasma). The active surface (graphene) of the device was functionalized with lactate dehydrogenase enzyme using different substances (Nafion, chitosan, and glutaraldehyde) to guarantee stability and increase selectivity. The devices presented linear responses for the concentration ranges tested in the different fluids. An interference study was performed using ascorbic acid, uric acid, and glucose, and there was a minimum variation in the Dirac point voltage during detection of lactate in any of the samples. The stability of the devices was verified at up to 50 days while kept in a dry box at room temperature, and device operation was stable until 12 days. This study demonstrated graphene performance to monitor L-lactic acid production in human samples, indicating that this material can be implemented in more simple and low-cost devices, such as flexible sensors, for point-of-care applications.

scholarly journals Dendrites Nanostructures of Co3O4 for the Selective Determination of Uric Acid

2021 ◽  
Author(s):  
Ramesh Lal ◽  
Bhajan Lal Bhattia ◽  
Ali M. Alsalme ◽  
Asma A. Al-Othman ◽  
Ayman Nafady ◽  
...  
Keyword(s):  
Composite Material ◽  
Uric Acid ◽  
Limit Of Detection ◽  
Low Cost ◽  
Phosphate Buffer Solution ◽  
Clinical Samples ◽  
Buffer Solution ◽  
Solution Ph ◽  
Dendrite Morphology ◽  
Selective Determination

Abstract The gout is mainly found due to accumulation of uric acid crystals into the joints which produces the inflammatory symptoms. Thus, it is highly demanded to detect uric acid from our body. Herein, we used wet chemical method for the preparation of composite material of cobalt oxide (Co3O4) with hydroxide functionalized multi-walled carbon nanotubes (MWCNTs). The analytical techniques such as scanning electron microscopy (SEM), powder X-ray diffraction (XRD), energy dispersive spectroscopy (EDS) and Infra-red spectroscopy (FTIR) were used to characterize the composite material. The Co3O4 exhibits a dendrite morphology and very well chemically coupled with MWCNTs. The elemental analysis confirmed the presence of Co, O and C as main constituent of the composite material. The Co3O4 exhibits a cubic unit cell crystallography in the composite system. The FTIR study has revealed the characteristic bands of Co-O bands in the composite material. The cyclic voltammetry was used to study the electrochemical properties of prepared materials. The composite sample with highest percentage of MWCNTs showed an excellent electrochemical activity towards the oxidation of uric acid in phosphate buffer solution pH 7.3. The enzyme free uric acid sensor possessed a linear range of 0.1 mM to 3 mM with a quantified limit of detection of 0.005 mM. The modified electrode is stable, selective, and very sensitive towards uric acid, therefore it may be used for the monitoring of uric acid from clinical samples. The proposed composite material is low cost, and earth abundant, thus it can be of great interest for energy and biomedical fields.

Synthesis and Characterization of an Aminated D-Glucose and its Stability of Cu2+Complex

2011 ◽  
Vol 197-198 ◽  
pp. 65-68 ◽  
Author(s):  
Qiang Qiang Liao ◽  
Yi Jiu Li ◽  
Bo Xiang
Keyword(s):  
Uv Spectra ◽  
Ultraviolet Region ◽  
Buffer Solution ◽  
Nmr Spectrum ◽  
Esi Ms ◽  
H Nmr ◽  
Ft Ir ◽  
The Stability ◽  
Complex Ratio

An aminated D-glucose [N,N'-di-b-D-glucopyranosyl ethylenediamine] was prepared and thoroughly characterized by FT-IR, ESI-MS, NMR spectra and elemental analysis. Compared with D-glucose, the FT-IR spectrum of the aminated glucose showed a moderate peak at 1629~1608 cm-1which was attributed to dNH vibration, suggesting glucose reacted to ethylenediamine (en). The ESI-MS spectrum exhibited a strong peak atm/z383.2, which was assigned to the species [C14H27O10N2]-. The1H-NMR spectrum of the aminated D-glucose demonstrated the signal of the C1proton and the amino proton at 4.82~4.79ppm, illustrating the amino of ethylenediamine was substituted for the hydroxy group of C1. As for UV spectra, the aminated glucose hadn’t absorbance in the ultraviolet region while its complex with Cu2+had obvious absorption peak at about 236nm. The complex ratio of the aminated glucose to Cu2+was close to 1:1 and the stability constant of its Cu2+complex was 6.8*107in 0.01mol×L-1borax buffer solution.

scholarly journals Simultaneous Adsorptive Stripping Voltammetric Analysis of Heavy Metals at Graphenated Cupferron Pencil Rods

2022 ◽  
Author(s):  
Nelia Abraham Sanga ◽  
Nazeem Jahed ◽  
Zandile Leve ◽  
Emmanuel Iwuoha ◽  
Keagan Pokpas
Keyword(s):  
Metal Ion ◽  
Tap Water ◽  
Low Cost ◽  
Simultaneous Detection ◽  
Chelating Agent ◽  
Acetate Buffer Solution ◽  
Buffer Solution ◽  
Solution Ph ◽  
Voltammetric Analysis ◽  
Electrochemically Reduced Graphene Oxide

Abstract Electroanalysis of heavy metal ions in the presence of cupferron ligands has been extensively studied due to its ability to form stable metallic coordination complexes. Herein, electrochemically reduced graphene oxide (ERGO) sheets were for the first time employed in conjunction with low-cost, disposable pencil graphite rods and in-situ plated thin mercury films (HgF) for the simultaneous detection of Cd2+, Cu2+, Pb2+, and Zn2+ in the presence of cupferron as a chelating agent by square-wave adsorptive cathodic stripping voltammetry (SW-AdCSV). The technique is based on the catalytic reduction of adsorbed cupferron-metal ion complexes at the surface of the ERGO-HgF-PGE at 0.1 V for 60 s in 0.1 M acetate buffer solution (pH 4.6). Owing to the improved electronic and surface effects associated with ERGO inclusion, improved sensitivity was further achieved. Under optimized conditions, the ERGO-HgF-PGE showed a linear relationship from 20 to 200 µg.L-1 with detection limits below the US-EPA of 0.17 μg.L-1 , 0.02 μg.L-1 , 0.17 μg.L-1 and 0.14 μg.L-1 for Cd2+, Cu2+, Pb2+ and Zn2+, respectively at a deposition time of 60 s. The ERGO-HgF-PGE exhibited highly reproducible results with negligible intermetallic interferences and applied successfully to the determination of trace metals in tap water with satisfactory results.

UV Curable Organic-Inorganic Hybrid Materials

2000 ◽  
Vol 628 ◽  
Author(s):  
Guang-Way Jang ◽  
Ren-Jye Wu ◽  
Yuung-Ching Sheen ◽  
Ya-Hui Lin ◽  
Chi-Jung Chang
Keyword(s):  
Hybrid Materials ◽  
Colloidal Silica ◽  
Sol Gel ◽  
Solution Ph ◽  
Uv Curable ◽  
Inorganic Hybrid ◽  
Degradation Temperature ◽  
Sol Gel Process ◽  
The Stability ◽  
Thermal Degradation Temperature

This work successfully prepared an UV curable organic-inorganic hybrid material consisting of organic modified colloidal silica. Applications of UV curable organic-inorganic hybrid materials include abrasion resistant coatings, photo-patternable thin films and waveguides. Colloidal silica containing reactive functional groups were also prepared by reacting organic silane and tetraethyl orthosilicate (TEOS) using sol-gel process. In addition, the efficiency of grafting organic moiety onto silica nanoparticles was investigated by applying TGA and FTIR techniques. Experimental results indicated a strong interdependence between surface modification efficiency and solution pH. Acrylate-SiO2 hybrid formation could result in a shifting of thermal degradation temperature of organic component from about 200°C to near 400°C. In addition, the stability of organic modified colloidal silica in UV curable formula and the physical properties of resulting coatings were discussed. Furthermore, the morphology of organic modified colloidal silica was investigated by performing TEM and SEM studies‥

Detection of Hepatitis a Virus in Drinking Water by Enzyme -Immunoassay Using Ultracentrifugation for Virus Concentration

1985 ◽  
Vol 17 (10) ◽  
pp. 39-41 ◽  
Author(s):  
A. Schnattinger
Keyword(s):  
Membrane Filtration ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Solid Phase ◽  
Phosphate Buffer Solution ◽  
Enzyme Linked Immunosorbent Assay ◽  
Virus Concentration ◽  
Buffer Solution ◽  
Solution Ph ◽  
Two Stage

Ten litres of tapwater were seeded with 200 µl (8×108 HAV particles) of a commercial (Organon Teknika) suspension of hepatitis A virus. Following WALTER and RÜDIGER (1981), the contaminated tapwater was treated with a two-stage technique for concentration of viruses from solutions with low virus titers. The two-stage technique consists of aluminium hydroxideflocculation (200 mg/l Al2(SO4)3. 18 H2O, pH 5,4-5,6) as first stage, the second stage of a lysis of aluminium hydroxidegel with citric acid/sodium citrate-buffer (pH 4,7; 1 ml/l sample), separation of viruses from the lysate by ultracentrifugation and suspension in 1 ml phosphate buffer solution (pH 7,2). A commercial solid phase enzyme-linked immunosorbent assay (ELISA) was used for the detection of HAV. HAV was detecterl in the 10.000:1 concentrates, but not in the seeded 101 samples. Approximately 4×108 of the inoculated 8×108 HAV particles were found in the 1 ml concentrates. The efficiency of detection is about 50%, the virus concentration 5000-fold. Although the percentage loss of HAV in comparison with concentration by means of membrane filtration is similar, the ultracentrifugation method yields a larger sample/concentrate ratio, so that smaller amounts of HAV can be detected more efficiently because of the smaller end-volume.

Sign in / Sign up

Export Citation Format

Share Document