scholarly journals IDENTIFIKASI SENYAWA FLAVONOID PADA FRAKSI ETIL ASETAT BENALU BATU (Begonia sp.) ASAL KABUPATEN MOROWALI UTARA

2016 ◽  
Vol 2 (2) ◽  
pp. 83-89
Author(s):  
Agus Ritna ◽  
Syariful Anam ◽  
Akhmad Khumaidi
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Preparative Thin Layer Chromatography ◽  
Ethyl Acetate Fraction ◽  
Visible Spectroscopy ◽  
Flavonoid Compounds ◽  
The People ◽  
Color Reagent ◽  
Vacuum Liquid Chromatography ◽  
Layer Chromatography

Benalu Batu Plant (Begonia sp.) in family Begoniaceae is a plant used by the people of North Morowali to treat tumors and cancers. It generally contains saponins, tannins, flavonoids and polyphenols. This research aimed to identify flavonoid compounds contained in ethyl acetate fraction of Begonia sp. The simplicia was extracted using maceration method and partitioned using n-hexane, ethyl acetate and water. The Ethyl acetate extract was then fractionated by nine eluent combinations using separation method of Vacuum Liquid Chromatography (VLC) and Preparative Thin Layer Chromatography (Prep TLC). One fraction was obtained and was suspected to contain flavonoid compounds after Shinode and Pew color reagent test. In the result of Ultraviolet-Visible Spectroscopy with methanol at wavelength range of 200-550 nm, the isolates showed absorption peaks at 275 nm (peak 1) and 225 nm (peak 2). Based on the wavelength of flavonoid compounds contained in the fraction, Begonia sp. showed similarities to the absorption peak of flavan-3-ol or flavanols

scholarly journals Isolation of Four Flavanones from the Leaves of Macaranga Hypoleuca (Rchb.f. & Zoll.) Müll.Arg

2019 ◽  
Vol 7 (4.14) ◽  
pp. 206
Author(s):  
H. Muhamad Nazeri ◽  
N. Ahmat ◽  
K. Abdul Khalil
Keyword(s):  
Ethyl Acetate ◽  
Column Chromatography ◽  
Preparative Thin Layer Chromatography ◽  
Ethyl Acetate Fraction ◽  
2D Nmr ◽  
Tropical Region ◽  
Pure Compounds ◽  
Large Genus ◽  
Vacuum Liquid Chromatography ◽  
Layer Chromatography

Macaranga is a large genus belongs to Euphorbiaceae family which commonly distributed in the tropical region of Africa, South-East Asia, China, and India. The plant of this genus contain flavonoids and stilbenes bearing various terpenyl groups including prenyl, geranyl and farnesyl groups. The leaves of M. hypoleuca were collected from reserved forest UiTM Jengka Pahang, Malaysia, and the dried powdered leaves were macerated in methanol at room temperature. The crude methanol extract was subjected to liquid-liquid partition using n-hexane and ethyl acetate to obtain hexane, ethyl acetate and aqueous fractions. The ethyl acetate fraction was semi purified using vacuum liquid chromatography (VLC) to give nine major fractions (MHL1-MHL9). Fraction MHL3 was further purified by column chromatography (CC) and preparative thin layer chromatography (p-TLC) to give two pure compounds, 8-prenylnaringenin (1) and sakuranetin (5,4’-dihydroxy-7-methoxyflavanone) (3). Meanwhile, fraction MHL6 was purified using column chromatography (CC) and p-TLC to yield another two pure compounds, 6-(3-hydroxy-3-methyl)naringenin (2) and 7-O-methyleriodictyol (4). The chemical structure of these isolated compounds were determined based on their 1D and 2D NMR, UV, and IR data. From this study, four flavanones were isolated from the leaves of M. hypoleuca.  

scholarly journals ISOLATION AND IDENTIFICATION OF β-SITOSTEROL AND STIGMASTEROL MIXTURE FROM ROOT BARK OF SLATRI (Calophyllum soulattri Burm. f)

2018 ◽  
Vol 10 (1) ◽  
pp. 87
Author(s):  
Doni Eko Saputra ◽  
Nestri Handayani ◽  
Muhammad Widyo Wartono
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Flash Chromatography ◽  
Root Bark ◽  
Isolation And Identification ◽  
H Nmr ◽  
Vacuum Liquid Chromatography ◽  
Layer Chromatography ◽  
Yellowish White ◽  
C Nmr

<p>Chemical isolation and identificationof root bark of <em>Calophyllumsoulattri</em>Burm. f. have been conducted. Isolation was conducted by maceration with ethyl acetate as solvent. Ethyl acetate extract was separated and purified by vacuum liquid chromatography and flash chromatography which was guided by thin layer chromatography to obtain yellowish white solid. Isolated compounds were determined by spectroscopy methods such as UV, FTIR, <sup>1</sup>H NMR, <sup>13</sup>C NMR, DEPT, HSQC, HMBC and were compared to the references. Based on the analysis result it’s known that the compounds are the mixture of?-sitosterol and stigmasterol.</p>

scholarly journals Isolation and Identification of DPPH Radical (2,2-diphenyl-1-pikrylhidrazyl) Scavenging Active Compound in Ethyl acetat fraction of Piper acre Blume

2019 ◽  
Vol 24 (3) ◽  
pp. 204
Author(s):  
Hifdzur Rashif Rijai ◽  
Nanang Fakhrudin ◽  
Subagus Wahyuono
Keyword(s):  
Ethyl Acetate ◽  
Active Compound ◽  
Pharmaceutical Preparations ◽  
Ethyl Acetate Fraction ◽  
Structure Identification ◽  
Isolation And Identification ◽  
East Kalimantan ◽  
The People ◽  
Ic50 Value ◽  
Vacuum Liquid Chromatography

Piper acre Blume, known as Black Betel (local name), is a plant that is widely used by the people of East Kalimantan, especially in Samarinda, for the treatment of illness. Leaves (3-4 months old) are collected from Samarinda, extracted, fractionated, and monitored by DPPH antiradical activity. The isolation of the Piper acre Blume is performed on the active fraction, and the structure identification is based on spectroscopic data of the compound.  The leaves were dried, pulverized, and macerated with MeOH. Dried MeOH extract was obtained upon evaporation of the solvent. The extract was then fractionated by vacuum liquid chromatography (vlc), eluted gradually by solvents having different polarities (n-hexane, ethyl acetate and methanol). The fractions obtained were monitored using TLC [n-hexane: ethyl acetate (3: 1 v/v)] that was visualized by UV254 nm, UV366 nm and DPPH. The isolation was performed by preparative TLC [SiO2, n-hexane: ethyl acetate (3: 1)] on ethyl acetate fraction that showed the highest DPPH antiradical value. A single compound was obtained, and it appeared as a round spot and pure according to TLC performances at 3 different solvent systems. The isolated Piper acre Blume compound displayed the IC50 value on the anti-radical DPPH (measured at λ 520 nm) as 10.41µg/mL. The IR spectrum (KBr) showed –OH band (3450 cm-1), aliphatic bands [alkene, 3010 cm-1; alkana 2900 cm-1), an aromatic overtone bands (1900-200 cm-1) and a strong C=O band (1725 cm-1). The NMR (1H- and 13C-) (mono and 2D) indicated the present of a p-di-substituted aromatic signals (δ, 7.54 and 7.52, d, J =6 Hz, 1 H each), 2 methyl (δ, 0.96, d, J = 7.0 Hz, 6 Hs), a triplet signal (δ, 4.22 ppm). Other signals of CH- and CH2 were shown as m signals at δ, 1.64 and 1.34 ppm.  Based on those data, the compound was identified as isoamyl p-OH benzoate that is grouped as parabens used as a preservative in the pharmaceutical preparations. In conclusion, the anti-radical (DPPH) active compound present in the leaves of Piper acre Blume is identified as isoamyl p-OH benzoate, having IC50 value anti-radical DPPH 10,41µg/mL.

scholarly journals Antioxidant Activity of Daemonorops draco Resin

2019 ◽  
Vol 22 (5) ◽  
pp. 179-183 ◽  
Author(s):  
Sri Purwanti ◽  
Wulan Tri Wahyuni ◽  
Irmanida Batubara
Keyword(s):  
Antioxidant Activity ◽  
Infrared Spectroscopy ◽  
Thin Layer ◽  
Ethyl Acetate ◽  
Thin Layer Chromatography ◽  
Column Chromatography ◽  
Ethyl Acetate Extract ◽  
Preparative Thin Layer Chromatography ◽  
Layer Chromatography

Jernang resin is secretion of jernang rattan (Daemonorops draco, Arecaceae family) fruits which is endemic in Southeast Asia. This resin has various biological activities and empirically used as wound healing, headache medicines, and fever remedies by Anak Dalam ethnic group from Jambi. This study was performed to evaluate the antioxidant activity of nonpolar fraction of D. draco resin which collected from Jambi Province, Sumatera, Indonesia. Resin was extracted with n-hexane, ethyl acetate, and methanol respectively. The antioxidant properties of the extracts were then evaluated using 1,1-diphenyl-2picryl-hidrazyl radical scavenging assay. The most active extract was further fractionated using n-hexane and methanol and separated using column chromatography and preparative thin layer chromatography. Separation of the extract was conducted through antioxidant assay-guided fractionation. Characterization of the active fraction was carried out by infrared spectroscopy. The result shows that ethyl acetate extract provides higher antioxidant activity (IC50 = 27.61 µg/mL) compare to methanol and n-hexane extracts. N-hexane fraction of ethyl acetate extract used for further separation using column and preparative thin layer chromatography due to its antioxidant activity. Separation using column chromatography resulting in 9 fractions (F.1-9). Fraction F.5 provide high antioxidant activity (IC50 = 17.27 µg/mL) and further separated using preparative thin layer chromatography resulting two fractions with lower antioxidant activity F.5.1 (IC50 = 85.18 µg/mL) and F.5.2 (IC50 = 34.94 µg/mL). Characterization of fraction F.5.2 using infrared spectroscopy showed that component in fraction F.5.2 contains NH-substituted benzene.

scholarly journals ISOLATION AND IDENTIFICATION OF β-SITOSTEROL AND STIGMASTEROL MIXTURE FROM ROOT BARK OF SLATRI (Calophyllum soulattri Burm. f)

2016 ◽  
Vol 10 (1) ◽  
pp. 87
Author(s):  
Doni Eko Saputra ◽  
Nestri Handayani ◽  
Muhammad Widyo Wartono
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Flash Chromatography ◽  
Root Bark ◽  
Isolation And Identification ◽  
H Nmr ◽  
Vacuum Liquid Chromatography ◽  
Layer Chromatography ◽  
Yellowish White ◽  
C Nmr

<p>Chemical isolation and identificationof root bark of <em>Calophyllumsoulattri</em>Burm. f. have been conducted. Isolation was conducted by maceration with ethyl acetate as solvent. Ethyl acetate extract was separated and purified by vacuum liquid chromatography and flash chromatography which was guided by thin layer chromatography to obtain yellowish white solid. Isolated compounds were determined by spectroscopy methods such as UV, FTIR, <sup>1</sup>H NMR, <sup>13</sup>C NMR, DEPT, HSQC, HMBC and were compared to the references. Based on the analysis result it’s known that the compounds are the mixture of?-sitosterol and stigmasterol.</p>

scholarly journals α-Amylase Inhibition Activities by Flavonoid Compounds from Panda Plants (Kalanchoe tomentosa)

2020 ◽  
Vol 23 (3) ◽  
pp. 96-101 ◽  
Author(s):  
Lilis Siti Aisyah ◽  
Delia Ilfani ◽  
Faridia Puspita Lestari ◽  
Yenny Febriani Yun
Keyword(s):  
Diabetes Mellitus ◽  
Ethyl Acetate ◽  
Preparative Thin Layer Chromatography ◽  
Ethyl Acetate Fraction ◽  
Glucose Absorption ◽  
Major Health ◽  
Ic50 Value ◽  
Layer Chromatography

Diabetes mellitus (DM) is one of the major health problems. One way to deal with diabetes mellitus is by inhibiting the work of enzymes that hydrolyze carbohydrates, thereby reducing glucose absorption. The enzyme that plays a role in the breakdown of starch into simple sugars is the α-amylase. The genus Kalanchoe or Panda plant has been widely reported to contain a variety of secondary metabolites that have several activities such as antimalarial, antibacterial, antidiabetic, and anticancer potential. This study aims to isolate, determine the chemical structure, and test the inhibitory activity of α-amylase from the ethyl acetate fraction of K. tomentosa leaves. The isolation stage began with the maceration process with n-hexane and fractionated with ethyl acetate. Ethyl acetate extract was refracted using a gradient liquid vacuum chromatography (KCV) method (n-hexane: ethyl acetate: methanol). Repeated gravity columns separated the fraction of KCV results and preparative thin-layer chromatography (TLC-P) to obtain isolate pure. Isolate pure was characterized using several spectroscopic methods, including UV-Vis, IR, NMR. Determination of the structure of the compound against isolate pure shows that this isolate is a 3,7,4’-trihydroxyflavonol or kaempferol compound. The results of the test activity of 3,7,4’-trihydroxyflavonol compounds in inhibiting the enzyme α-amylase in vitro showed an IC50 value of 346 µU/mL. The results obtained indicate that the IC50 value is higher than that of the Acarbose, which was 39.3 µU/mL.

scholarly journals ISOLATION AND IDENTIFICATION OF FLAVONOID COMPOUND EXTRACTIRE ETHYL ACETATE FRACTION EXTRACTED FROM THE RHIZOMES FINGERROOT OF (Boesenbergia pandurata (Roxb.) Schlecht) (Zingiberaceae)

2010 ◽  
Vol 6 (2) ◽  
pp. 219-223 ◽  
Author(s):  
Ochtavia Prima Sari ◽  
Titik Taufiqurrohmah
Keyword(s):  
Liquid Chromatography ◽  
Melting Point ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Ethyl Acetate Fraction ◽  
Flavonoid Compound ◽  
Isolation And Identification ◽  
Ft Ir ◽  
Vacuum Liquid Chromatography ◽  
Isolated Compound

Boesenbergia pandurata (Roxb.) Schlecht is one of fingerroot plant in ginger family (Zingiberaceae). The rhizomes of the plant contained a lot of secondary metabolites compounds. Therefore, the purpose of the research is to isolate and identify the flavonoid compound from the plant. The rhizomes were extracted with metanol continued by partition using ethyl acetate-water (1:1). The ethyl acetate extract was chromatographed on a column of Si gel (Vacuum Liquid Chromatography and Gravitation Column Chromatography) using n-hexane-ethyl acetate (5:2) as eluents. Further purification by recristalization  using benzene  produced a compound as yellow powder (16 mg) having melting point of 294-295 oC. The spectra of isolated compound were determined by spectroscopic UV-Vis, FT-IR, and GC-MS. Spectrum UV-Vis of the isolated compound showed ultraviolet absorption at λmax (MeOH, nm) 290 and 322; λmax (MeOH+NaOH, nm) 322; λmax (MeOH+AlCl3, nm) 309; λmax (MeOH+AlCl3+HCl, nm) 310; λmax (MeOH+NaOAc, nm) 322 and λmax (MeOH+NaOAc+H3BO3, nm) 290. Its FT-IR spectrum represented a number of absorption lied on νmax (cm-1) : 3142.5; 3012.6; 2893; 2345.3; 1631.7; 1585.4; 1357.8; 1168.8; and 825.5. GC-MS spectrum of the isolated compound exhibited an [M]+ ion peak at m/z = 256 with retention time of  22,579. Based on the results of spectrum analysis it can be concluded that the compound is 5,7-dyhydroxyflavanone.   Keywords: 5,7-dyhydroxyflavanone, Boesenbergia pandurata, ethyl asetat, fingerroot

Isolation of the antidiarrhoeal tiliroside and its derivative from Waltheria indica leaf extract

2021 ◽  
Vol 25 (1) ◽  
pp. 86-92
Author(s):  
B.A. Ayinde ◽  
J.O. Owolabi ◽  
I.S. Uti ◽  
P.C. Ogbeta ◽  
M.I. Choudhary
Keyword(s):  
Silica Gel ◽  
Column Chromatography ◽  
Methanol Extract ◽  
Preparative Thin Layer Chromatography ◽  
Inhibitory Effects ◽  
Aqueous Fraction ◽  
Vacuum Liquid Chromatography ◽  
Ileum Contraction ◽  
Layer Chromatography ◽  
Dose Dependent

The antidiarrhoeal effect of Waltheria indica methanol extract and fractions have been reported earlier but, the present work examined the intestinal relaxant effects of two flavonoid-phenyl propanoids isolated from the methanol extract. The active aqueous fraction was subjected to vacuum liquid chromatography using dichloromethane with increasing concentration of ethyl acetate, and that of methanol and water successively. The ten (10) fractions obtained were combined to give seven (7). The fraction 2 (C, D) was subjected to preparative thin layer chromatography on silica gel GF254 (10-40μm) using CHCl3-CH3OH (8:2) to obtain compound coded F2. Fraction 4 (F) was subjected to column chromatography using silica gel (60-120μm mesh) and eluted with  dichloromethane with increasing concentrations of methanol. Fractions 9-28 were combined and subjected to column  chromatography using chloroform with increasing concentration of methanol. The fractions 1-16 of these were purified on Sephadex LH-20 to obtain compound BAA. The identities of the two compounds were established using spectroscopic methods. The  antidiarrheal effect of compound F2 was evaluated on mice using charcoal transit (100,200, 400mg/kg), castor oil (40, 60 mg/kg)  while the two compounds were examined for their inhibitory effects on Ach-induced ileum contraction. The effects of the  compounds were compared with loperamide (3mg/kg) and atropine (80μg). Compounds F2 and BAA were identified as tiliroside and 3’’’, 5’’’-dimethoxy tiliroside respectively. Tiliroside inhibited the charcoal transition in the animals in a dose dependent pattern with 400mg/ mL eliciting 63.41% inhibition compared to 59.23% produced by loperamide. The compound also elicited significantly (P<0.05) prolonged onset of stooling and reduced the number and weight of stools produced lower than the control. The two  compounds drastically inhibited the Ach-induced contractions of the ileum. The compound, tiliroside at 10mg, completely abolished  the contraction by Ach unlike 3’’’, 5’’’-dimethoxy tiliroside which reduced the contraction to 1.92% at 20mg. The identified compounds seem to be responsible for the ethnomedicinal use of the plant in treating diarrhea.

scholarly journals ANTIOXIDANT ACTIVITY OF RED DRAGON FRUIT PEEL (HYLOCEREUS POLYRHIZUS (F.A.C. WEBER) BRITTON AND ROSE) ISOLATES USING 2,2-DIPHENYL-1-PICRYLHYDRAZYL METHOD

2018 ◽  
Vol 11 (1) ◽  
pp. 124 ◽  
Author(s):  
Sri Wahdaningsih ◽  
Subagus Wahyuono ◽  
Sugeng Riyanto ◽  
Retno Murwanti
Keyword(s):  
Antioxidant Activity ◽  
Liquid Chromatography ◽  
Medicinal Plants ◽  
Thin Layer ◽  
Preparative Thin Layer Chromatography ◽  
Fruit Peel ◽  
Dragon Fruit ◽  
Vacuum Liquid Chromatography ◽  
Layer Chromatography ◽  
Hylocereus Polyrhizus

  Objective: Indonesia is a country which has various natural resources including medicinal plants. Among the plants, red dragon fruit is interesting to be studied. In this study, antioxidant activity of red dragon fruit peel (Hylocereus polyrhizus (F.A.C. Weber) Britton and Rose) isolate was tested using 2,2-diphenyl-1-picrylhydrazyl method.Methods: Isolation of active compounds was performed through the application of vacuum liquid chromatography and preparative thin-layer chromatography (TLC). Antioxidant activity was tested using both TLC and spectrophotometry.Result: Results revealed that the values of IC50 of isolate 1 and 2 were 2.952, 14 μg/mL and 25.635,95 μg/mL, respectively.Conclusion: Isolate of dragon fruit has antioxidant activity. The compound which results in antioxidant activity is terpenoid and steroid.

scholarly journals Comparative phytotoxicity and chironomid toxicity assessments of leaf successive extract fractions of Asiatic poison bulb, Crinum asiaticum L

2020 ◽  
Author(s):  
Souren Goswami ◽  
Sanjib Ray
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Ethyl Acetate Extract ◽  
Antioxidant Properties ◽  
Ethyl Acetate Fraction ◽  
Meristematic Tissue ◽  
Aqueous Fraction ◽  
Extract Fraction ◽  
Potent Growth ◽  
Crinum Asiaticum

AbstractCrinum asiaticum is an evergreen bulbous perennial shrub of Amaryllidaceae family with ethnomedicinal importance and our earlier study described a comparative account antimicrobial and antioxidant properties of the different solvent-mediated sequential extract fractions. The present study aimed to analyze their comparative account of induced phytotoxicity and chironomid toxicity. For phytotoxicity assessment, germination inhibition and seedling’s root and shoot growth retardation effects on Triticum aestivum and Cicer arietinum were analyzed and for lethal concentration determination, the freshwater bottom-dwelling first instars chironomid larvae were used. The crude aqueous, petroleum ether and ethyl acetate extracts showed significant toxic effects on both meristematic tissue and aquatic midges. The phytotoxic assays indicate that the ethyl acetate fraction contains the most potent growth inhibitors, followed by the crude aqueous and petroleum ether fractions. The last aqueous fraction was found to be the least toxic, the highest LC50 and LT50 values and ethyl acetate extract fraction having highest toxicity. Thus the present study supplements to our earlier report, that indicated the last aqueous extract fraction of C. asiaticum has potent antioxidant and antibacterial potentials as well as its prospective use in livestock maintenance, as it is least toxic and the ethyl acetate extract, the most toxic fraction identified here, is needed to explore for pharmaceutical importance.

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