scholarly journals ISOLATION AND IDENTIFICATION OF FLAVONOID COMPOUND EXTRACTIRE ETHYL ACETATE FRACTION EXTRACTED FROM THE RHIZOMES FINGERROOT OF (Boesenbergia pandurata (Roxb.) Schlecht) (Zingiberaceae)

2010 ◽  
Vol 6 (2) ◽  
pp. 219-223 ◽  
Author(s):  
Ochtavia Prima Sari ◽  
Titik Taufiqurrohmah
Keyword(s):  
Liquid Chromatography ◽  
Melting Point ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Ethyl Acetate Fraction ◽  
Flavonoid Compound ◽  
Isolation And Identification ◽  
Ft Ir ◽  
Vacuum Liquid Chromatography ◽  
Isolated Compound

Boesenbergia pandurata (Roxb.) Schlecht is one of fingerroot plant in ginger family (Zingiberaceae). The rhizomes of the plant contained a lot of secondary metabolites compounds. Therefore, the purpose of the research is to isolate and identify the flavonoid compound from the plant. The rhizomes were extracted with metanol continued by partition using ethyl acetate-water (1:1). The ethyl acetate extract was chromatographed on a column of Si gel (Vacuum Liquid Chromatography and Gravitation Column Chromatography) using n-hexane-ethyl acetate (5:2) as eluents. Further purification by recristalization  using benzene  produced a compound as yellow powder (16 mg) having melting point of 294-295 oC. The spectra of isolated compound were determined by spectroscopic UV-Vis, FT-IR, and GC-MS. Spectrum UV-Vis of the isolated compound showed ultraviolet absorption at λmax (MeOH, nm) 290 and 322; λmax (MeOH+NaOH, nm) 322; λmax (MeOH+AlCl3, nm) 309; λmax (MeOH+AlCl3+HCl, nm) 310; λmax (MeOH+NaOAc, nm) 322 and λmax (MeOH+NaOAc+H3BO3, nm) 290. Its FT-IR spectrum represented a number of absorption lied on νmax (cm-1) : 3142.5; 3012.6; 2893; 2345.3; 1631.7; 1585.4; 1357.8; 1168.8; and 825.5. GC-MS spectrum of the isolated compound exhibited an [M]+ ion peak at m/z = 256 with retention time of  22,579. Based on the results of spectrum analysis it can be concluded that the compound is 5,7-dyhydroxyflavanone.   Keywords: 5,7-dyhydroxyflavanone, Boesenbergia pandurata, ethyl asetat, fingerroot

scholarly journals Isolasi dan Identifikasi Metabolit Sekunder dari Ekstrak Etil Asetat Batang Nangka (Artocarpus heterophyllus Lmk.) dan Aktivitasnya Sebagai Antibakteri

KOVALEN ◽  
2020 ◽  
Vol 6 (1) ◽  
pp. 81-89
Author(s):  
Indriani ◽  
Iswan
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Flavonoid Compound ◽  
Diffusion Method ◽  
Inhibitory Zone ◽  
Isolation And Identification ◽  
Artocarpus Heterophyllus ◽  
Isolated Compound ◽  
Concentration Series

Isolation and identification of secondary metabolite of ethyl acetate extract of jackfruit stem (Artocarpus heterophyllus Lmk.) as well as its activity as antibacterial have been carried out. The purpose of this study was to know the characterization and antibacterial activity of secondary metabolites from ethyl acetate extract of the Jackfruit stem. Ethyl acetate extract was fractionated and purified using several chromatographic techniques. The identification of the isolated compound was elucidated by spectroscopic UV-Vis, IR, and NMR. Based on data analysis of UV, IR, and NMR spectra showed that the isolated compound was a flavonoid derivative. Antibacterial activity test of the isolated compound to Escherichia coli and Staphylococcus aureus was done by a well diffusion method. The inhibitory zone values of flavonoid compound in concentration series of 1000 µg/ml, 3000 µg/ml, and 5000 µg/ml against E. coli were 9.65 mm, 10.25 mm, and 11.00 mm, respectively. While, the inhibitory zone values in the same concentration series against  S. aureus were 9.00 mm, 10.75 mm, and 11.38 mm.  Keywords: Jackfruit, Artocarpus heterophyllus Lmk., flavonoid, antibacterial activity.

scholarly journals Isolation and Identification of Secondary Metabolite from Marine Sponge Callyspongia sp. and its Antibacterial Potency

2020 ◽  
Vol 11 (3) ◽  
pp. 10082-10088
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Marine Sponge ◽  
Ethyl Acetate Extract ◽  
Aldehyde Group ◽  
Isolation And Identification ◽  
E Coli ◽  
Microdilution Method ◽  
Antibacterial Susceptibility ◽  
Vacuum Liquid Chromatography

Marine sponge Callyspongia sp. is one full of potency as a source for discovering and developing novel antibacterial. This study aims to isolate the Callypsongia sp. and assay their antibacterial activity. Callyspongia sp. were macerated with ethyl acetate (3x24 hrs), isolated with vacuum liquid chromatography (VLC) and RC (radial chromatography), and determined their structure with 1H and 13C-NMR. The antibacterial activity was assayed with the microdilution method. From ethyl acetate extract of Callyspongia sp. was successfully 2 isolated compounds, namely, isolate C1 (cholesterol) and isolate C2 (Unknown alkaloid with carbonyl from aldehyde group). The extract has MIC>512 µg/mL against Bacillus subtilis, Escherichia coli, Streptococcus mutans, and Salmonella enterica. While in both isolates provided MIC value >256 µg/mL against B. subtilis, E. coli, and S. mutans, yet in S. enterica provided 128 µg/mL for isolate C1 and 256 µg/mL for isolate C2. In conclusion, ethyl acetate extract of Callyspongia sp. contains cholesterol and Unknown alkaloid with carbonyl from the aldehyde group, and they both exhibited low antibacterial susceptibility.

scholarly journals IDENTIFIKASI SENYAWA FLAVONOID PADA FRAKSI ETIL ASETAT BENALU BATU (Begonia sp.) ASAL KABUPATEN MOROWALI UTARA

2016 ◽  
Vol 2 (2) ◽  
pp. 83-89
Author(s):  
Agus Ritna ◽  
Syariful Anam ◽  
Akhmad Khumaidi
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Preparative Thin Layer Chromatography ◽  
Ethyl Acetate Fraction ◽  
Visible Spectroscopy ◽  
Flavonoid Compounds ◽  
The People ◽  
Color Reagent ◽  
Vacuum Liquid Chromatography ◽  
Layer Chromatography

Benalu Batu Plant (Begonia sp.) in family Begoniaceae is a plant used by the people of North Morowali to treat tumors and cancers. It generally contains saponins, tannins, flavonoids and polyphenols. This research aimed to identify flavonoid compounds contained in ethyl acetate fraction of Begonia sp. The simplicia was extracted using maceration method and partitioned using n-hexane, ethyl acetate and water. The Ethyl acetate extract was then fractionated by nine eluent combinations using separation method of Vacuum Liquid Chromatography (VLC) and Preparative Thin Layer Chromatography (Prep TLC). One fraction was obtained and was suspected to contain flavonoid compounds after Shinode and Pew color reagent test. In the result of Ultraviolet-Visible Spectroscopy with methanol at wavelength range of 200-550 nm, the isolates showed absorption peaks at 275 nm (peak 1) and 225 nm (peak 2). Based on the wavelength of flavonoid compounds contained in the fraction, Begonia sp. showed similarities to the absorption peak of flavan-3-ol or flavanols

scholarly journals ISOLATION AND IDENTIFICATION OF β-SITOSTEROL AND STIGMASTEROL MIXTURE FROM ROOT BARK OF SLATRI (Calophyllum soulattri Burm. f)

2018 ◽  
Vol 10 (1) ◽  
pp. 87
Author(s):  
Doni Eko Saputra ◽  
Nestri Handayani ◽  
Muhammad Widyo Wartono
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Flash Chromatography ◽  
Root Bark ◽  
Isolation And Identification ◽  
H Nmr ◽  
Vacuum Liquid Chromatography ◽  
Layer Chromatography ◽  
Yellowish White ◽  
C Nmr

<p>Chemical isolation and identificationof root bark of <em>Calophyllumsoulattri</em>Burm. f. have been conducted. Isolation was conducted by maceration with ethyl acetate as solvent. Ethyl acetate extract was separated and purified by vacuum liquid chromatography and flash chromatography which was guided by thin layer chromatography to obtain yellowish white solid. Isolated compounds were determined by spectroscopy methods such as UV, FTIR, <sup>1</sup>H NMR, <sup>13</sup>C NMR, DEPT, HSQC, HMBC and were compared to the references. Based on the analysis result it’s known that the compounds are the mixture of?-sitosterol and stigmasterol.</p>

scholarly journals Isolation, Characterization and Antimicrobial Activity of Quercetin-3-O-(2”-α-Methyl-p-Coumaryl)-Rutinoside from Delonix elata Flowers

2020 ◽  
pp. 1-11
Author(s):  
A. Rajeshkanna ◽  
M. M. Senthamilselvi ◽  
D. Prabhakaran
Keyword(s):  
Antimicrobial Activity ◽  
Ethyl Acetate ◽  
Research Work ◽  
Ethyl Acetate Fraction ◽  
Salmonella Typhi ◽  
Flavonoid Compound ◽  
Diffusion Method ◽  
Curvularia Lunata ◽  
Bacterial Strains ◽  
Isolated Compound

Aims: To investigate the isolated flavonoid compound, characterization and antimicrobial activity of Quercetin-3-O-(2”-α-methyl-p-Coumaryl)-rutinoside from Delonix elata flowers. Place and Duration of Study: The research work was carried out at Research laboratory, Department of chemistry, Periyar E.V.R College, Trichy-23, between May 2016 to January 2018. Methodology: Extraction and fractionation was carried out from the solvents of ethanol, benzene, petroleum ether, diethyl ether and ethyl acetate. The structure of the isolated compound (Quercetin-3-O-(2”-α-methyl-p-Coumaryl)-rutinoside) was elucidated through their physical and chemical methods. The isolated compound was characterized by using various spectral data such as UV, 1H NMR, 13C NMR, MS. Four bacterial strains Salmonella typhi, Escherichia coli, Enterococcus faecalis, Bacillus cereus and two fungal strains Curvularia lunata and Candida albicans were tested by using disc diffusion method. Conclusion: The present study was concluded that the dry sample of ethyl acetate fraction of Delonix elata flowers was Quercetin-3-O-(2”-α-methyl-p-Coumaryl)-rutinoside and it possesses effective antimicrobial activity against bacteria and fungi.

scholarly journals Isolation and Identification of DPPH Radical (2,2-diphenyl-1-pikrylhidrazyl) Scavenging Active Compound in Ethyl acetat fraction of Piper acre Blume

2019 ◽  
Vol 24 (3) ◽  
pp. 204
Author(s):  
Hifdzur Rashif Rijai ◽  
Nanang Fakhrudin ◽  
Subagus Wahyuono
Keyword(s):  
Ethyl Acetate ◽  
Active Compound ◽  
Pharmaceutical Preparations ◽  
Ethyl Acetate Fraction ◽  
Structure Identification ◽  
Isolation And Identification ◽  
East Kalimantan ◽  
The People ◽  
Ic50 Value ◽  
Vacuum Liquid Chromatography

Piper acre Blume, known as Black Betel (local name), is a plant that is widely used by the people of East Kalimantan, especially in Samarinda, for the treatment of illness. Leaves (3-4 months old) are collected from Samarinda, extracted, fractionated, and monitored by DPPH antiradical activity. The isolation of the Piper acre Blume is performed on the active fraction, and the structure identification is based on spectroscopic data of the compound.  The leaves were dried, pulverized, and macerated with MeOH. Dried MeOH extract was obtained upon evaporation of the solvent. The extract was then fractionated by vacuum liquid chromatography (vlc), eluted gradually by solvents having different polarities (n-hexane, ethyl acetate and methanol). The fractions obtained were monitored using TLC [n-hexane: ethyl acetate (3: 1 v/v)] that was visualized by UV254 nm, UV366 nm and DPPH. The isolation was performed by preparative TLC [SiO2, n-hexane: ethyl acetate (3: 1)] on ethyl acetate fraction that showed the highest DPPH antiradical value. A single compound was obtained, and it appeared as a round spot and pure according to TLC performances at 3 different solvent systems. The isolated Piper acre Blume compound displayed the IC50 value on the anti-radical DPPH (measured at λ 520 nm) as 10.41µg/mL. The IR spectrum (KBr) showed –OH band (3450 cm-1), aliphatic bands [alkene, 3010 cm-1; alkana 2900 cm-1), an aromatic overtone bands (1900-200 cm-1) and a strong C=O band (1725 cm-1). The NMR (1H- and 13C-) (mono and 2D) indicated the present of a p-di-substituted aromatic signals (δ, 7.54 and 7.52, d, J =6 Hz, 1 H each), 2 methyl (δ, 0.96, d, J = 7.0 Hz, 6 Hs), a triplet signal (δ, 4.22 ppm). Other signals of CH- and CH2 were shown as m signals at δ, 1.64 and 1.34 ppm.  Based on those data, the compound was identified as isoamyl p-OH benzoate that is grouped as parabens used as a preservative in the pharmaceutical preparations. In conclusion, the anti-radical (DPPH) active compound present in the leaves of Piper acre Blume is identified as isoamyl p-OH benzoate, having IC50 value anti-radical DPPH 10,41µg/mL.

scholarly journals ISOLATION AND IDENTIFICATION OF β-SITOSTEROL AND STIGMASTEROL MIXTURE FROM ROOT BARK OF SLATRI (Calophyllum soulattri Burm. f)

2016 ◽  
Vol 10 (1) ◽  
pp. 87
Author(s):  
Doni Eko Saputra ◽  
Nestri Handayani ◽  
Muhammad Widyo Wartono
Keyword(s):  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Flash Chromatography ◽  
Root Bark ◽  
Isolation And Identification ◽  
H Nmr ◽  
Vacuum Liquid Chromatography ◽  
Layer Chromatography ◽  
Yellowish White ◽  
C Nmr

<p>Chemical isolation and identificationof root bark of <em>Calophyllumsoulattri</em>Burm. f. have been conducted. Isolation was conducted by maceration with ethyl acetate as solvent. Ethyl acetate extract was separated and purified by vacuum liquid chromatography and flash chromatography which was guided by thin layer chromatography to obtain yellowish white solid. Isolated compounds were determined by spectroscopy methods such as UV, FTIR, <sup>1</sup>H NMR, <sup>13</sup>C NMR, DEPT, HSQC, HMBC and were compared to the references. Based on the analysis result it’s known that the compounds are the mixture of?-sitosterol and stigmasterol.</p>

A Flavonoid compound of Turbinaria decurrens Bory with The Potential Antioxidant and Anticancer Activity

2021 ◽  
pp. 6207-6210
Author(s):  
Fitriyanti Jumaetri Sami ◽  
Nunuk Hariani Soekamto ◽  
Tatsufumi Okino ◽  
Firdaus Firdaus ◽  
Jalifah latip
Keyword(s):  
Ethyl Acetate ◽  
Anticancer Activity ◽  
Ethyl Acetate Extract ◽  
Flavonoid Compound ◽  
Mtt Method ◽  
South Sulawesi ◽  
Isolation And Characterization ◽  
Ic50 Value ◽  
Dpph Method ◽  
Ft Ir

Isolation and characterization of quercetin flavonoid compound from Dutungan Island, South Sulawesi Province, Indonesia has been successfully done from ethyl acetate extract. Extraction method used maceration, isolation used chromatography, anticancer activity with MTT method and antioxidant test used DPPH radical. Structure was discussed with the FT-IR, NMR spectrophotometer and compared with the literature. Total flavonoids from ethyl acetate extract were 4.8 mgEQ/g, IC50 value of antioxidant activity was 4.23 μg/ml using the DPPH method (2,2-diphenyl-1-picrylhydrazyl), and anticancer activity of H460 cells IC50 value was 10.95 μg/ml. The quercetin compound is potential as an anticancer and was first report in the T. decurrens Bory species.

In-vitro bactericidal activity of a novel plant source Plumeria pudica against some human and fish pathogenic bacteria

2020 ◽  
Vol 17 ◽  
Author(s):  
Shubhaisi Das ◽  
Sunanda Burman ◽  
Goutam Chandra
Keyword(s):  
Ethyl Acetate ◽  
Bioactive Compounds ◽  
Pathogenic Bacteria ◽  
Ethyl Acetate Extract ◽  
Positive Control ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Ft Ir ◽  
Aldehyde Groups

Background: The only remedy for up surging problem of antibiotic resistance is the discovery of antibacterial agents of natural origin. Objective: The present study was aimed at finding antibacterial potential of crude and solvent extracts of mature leaves of Plumeria pudica. Methods: Antibacterial activity of three different solvent extracts were evaluated in four human and four fish pathogenic bacteria by measuring the zone of inhibition and determining Minimum Inhibitory Concentration and Minimum Bactericidal Concentration values. Standard antibiotics were used as positive control. Preliminary phytochemical screening of most effective extract i.e., ethyl acetate extract, Fourier Transform Infra Red analysis and GC-MS analysis of the Thin Layer Chromatographic (TLC) fraction of ethyl acetate extract were done meticulously. All experiments were done thrice and analyzed statistically. Results: Crude leaf extracts and solvent extracts caused good inhibition of bacterial growth in all selected bacteria. Ethyl acetate extract showed highest inhibition zones in all tested strains with maximum inhibition (19.50±0.29 mm) in Escherichia coli (MTCC 739). MBC/MIC of the extracts indicated that all three solvent extracts were bactericidal. Preliminary phytochemical tests revealed the presence of tannins, steroids and alkaloids and FT-IR analysis revealed presence of many functional groups namely alcoholic, amide, amine salt and aldehyde groups. From the GC-MS analysis of TLC fraction of ethyl acetate extract five different bioactive compounds e.g., 2,4-ditert –butylphenyl 5-hydroxypentanoate, Oxalic acid; allyl nonyl ester, 7,9-Ditert-butyl-1-oxaspiro(4,5)deca-6,9-diene-2,8-dione, Dibutyl phthalate and 2,3,5,8-tetramethyl-decane were identified. Conclusion: Leaf extracts of P. pudica contain bioactive compounds that can be used as broad spectrum bactericidal agent.

scholarly journals Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches

2014 ◽  
Vol 86 (2) ◽  
pp. 881-888 ◽  
Author(s):  
MARIANA PIANA ◽  
ALINE A. BOLIGON ◽  
THIELE F. DE BRUM ◽  
MARINA ZADRA ◽  
BIANCA V. BELKE ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ethyl Acetate ◽  
Antioxidant Capacity ◽  
Chlorogenic Acid ◽  
Condensed Tannins ◽  
High Performance ◽  
Ethyl Acetate Fraction ◽  
Phytochemical Analysis ◽  
Dpph Method

The antioxidant capacity of the crude extract and fractions ofTabernaemontana catharinensis fruits and branches, was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the content of polyphenols, flavonoids, alkaloids and condensed tannins were determined by the spectrophotometric method. The ethyl acetate fraction of the fruits and the n-butanol fraction of the branches showed IC50 of 181.82 µg/mL and 78.19 µg/mL, respectively. All fractions were analyzed by high performance liquid chromatography (HPLC), in the branches were quantified chlorogenic acid in the chloroform (8.96 mg/g), ethyl acetate (4.31 mg/g) and n-butanol (3.33 mg/g) fractions; caffeic acid in the ethyl acetate (5.24 mg/g) and n-butanol (1.81 mg/g); gallic acid (0.52 mg/g) in the n-butanol. In the fruits, chlorogenic acid in the chloroform (1.67 mg/g); rutin in the ethyl acetate (3.45 mg/g) and n-butanol (8.98 mg/g) fractions. The present study showed that these quantified compounds can contribute to antioxidant capacity which was higher in the branches than in the fruits.

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