scholarly journals Kajian Kadar Akrilamida dalam Kopi Arabika dengan Variasi Suhu Penyangraian

KOVALEN ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 178-185
Author(s):  
Gatut Ari Wardani ◽  
Lilis Tuslinah ◽  
Eva Saefatuzzahro ◽  
Indra
Keyword(s):  
High Performance ◽  
Reversed Phase ◽  
Phosphoric Acid Solution ◽  
Homogeneity Test ◽  
Arabica Coffee ◽  
Anova Test ◽  
Normality Test ◽  
Bonferroni Test ◽  
Roasting Temperature ◽  
Post Hoc

Acrylamide levels in Arabica coffee at various roasting temperatures have been determined. Coffee beans are roasted at 160°C, 180°C, and 210°C for 8 minutes. Analysis was carried out using High-Performance Liquid Chromatography (HPLC) with C-18 reversed-phase, mobile phase using methanol and 0.1% phosphoric acid solution (5:95), flow rate 1 mL/min, UV-Vis detector 198 nm wavelength. Acrylamide levels in Arabica coffee are 160°C of 14.665 ppm, 180°C of 15.973 ppm, and 210°C of 18.501 ppm. Based on the results of the acrylamide content in Arabica coffee, the higher the roasting temperature, the greater the acrylamide content. ANOVA test regarding the normality test sig value of 0.637, 0.463, and 0.637 normally distributed. Based on the homogeneity test, it was found that the variation between groups was the same or homogeneous. The results of the ANOVA test stated that there were differences in the acrylamide levels of Arabica coffee in the roasting temperature difference group and the results of the post hoc Bonferroni test found that the average differences in the roasting temperature of Arabica coffee were significantly different 160°C-180°C and 160°C-210°C.

scholarly journals PENGARUH CARA PENGOLAHAN DAN WAKTU BERBEDA TERHADAP KADAR PROTEIN DAGING BEKICOT (ACHANTINA FULICA) SEBAGAI SUMBER PROTEIN ALTERNATIF MASYARAKAT NEGERI LESLURU KECAMATAN TEON NILA SERUA (TNS) WAIPIA

2019 ◽  
Vol 5 (2) ◽  
pp. 72-81
Author(s):  
Rofli J Lessu ◽  
Mery Pattipeilohy ◽  
Stevin Melay
Keyword(s):  
Protein Content ◽  
Experimental Research ◽  
Homogeneity Test ◽  
Nutritional Content ◽  
Alternative Source ◽  
Anova Test ◽  
Meat Protein ◽  
Normality Test

Background: Snail (Achantina fulica) is one of the animals from the gastropod class. Snails are often consumed by the Lesluru people as an alternative source of protein for fish substitutes. This study aims to determine the levels of snail meat protein which is processed by boiling, fumigation and frying, as well as providing information on the nutritional content of snails for the community in general. Method: The type of research used is experimental research and to test the protein content using the kjeldhal method. This research was conducted from November 4 to November 12, 2018. Results: This study was analyzed using the normality test, homogeneity test and followed by ANOVA test. In analyzing the data used the SPSS Windows version 16.0 program help. The results of the analysis of protein content of snail meat, known, the highest levels found in the treatment by fumigation with an average protein content of 40.4% while the lowest protein content found in boiling processing with an average protein content of 27.0%. Conclusion: treatment by fumigation while the lowest protein content is found in the treatment by boiling.

The effect of different levels of ozone and ozonized water on biogenic amines in broiler chicken meat by HPLC

Food Research ◽  
2021 ◽  
Vol 5 (2) ◽  
pp. 129-135
Author(s):  
A. Zakeri ◽  
O. Farzalipour ◽  
K. Kamyar Hidarnejad ◽  
P. Ahangar Parvin ◽  
S.H. Sadeghpour
Keyword(s):  
Biogenic Amines ◽  
High Performance ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Poultry Meat ◽  
Ozone Treatment ◽  
Group 2 ◽  
Experimental Treatments ◽  
Post Hoc ◽  
Group 1

Biogenic amines (BA) are low molecular weight substances, formed mainly by decarboxylation of specific amino acids present in food through the action of enzymes during storage produced by some microorganisms, this fact can be used to relate BA as a bacteriological quality indicator. This study was aimed to evaluate the effects of an experimental ozone gaseous treatment and production of the biogenic amines’ putrescine and cadaverine. Amines were extracted with perchloric acid, derivative with dansyl chloride, separated using a reversed-phase high-performance liquid chromatographic method, and detected by fluorescence. The results showed that during the 7 days, the amount of putrescine had increased in all four experimental treatments. The highest increase was related to Group 1. The Duncan post hoc test showed that the highest amount of Cadaverine after killing was related to Group 1 (control) with 88.85 mg/kg and the lowest value for Group 2 is 12.03 mg/kg. Also, the results of Duncan's post hoc test for comparing Cadaverine seven days after slaughter among experimental treatments showed that the highest amount of Cadaverine after slaughter was related to Group 1 (control) with 135.6 mg/kg and the lowest value for the group is 94.83 mg/kg. The results of the test to compare the pH of the treatments showed that the highest amount of pH is for Group 4 and the lowest value for Group 1 (control). The results of this study showed that with increasing ozone gas concentration and decreasing the concentration of water, the biogenic amines’ putrescine and cadaverine in frozen poultry meat decreased. Putrescine and cadaverine levels appeared to be useful to control the effectiveness of the ozone treatment on meat quality and may be useful as a quality index to highlight the loss of poultry meat freshness.

THE INFLUENCE ANALYSIS OF DIFFERRENTLY CONSTRUCTED FOLDED TRAPS AND TYPES OF BAITS TO CATCH CRABS [Portunus pelagicus, (Linnaeus,1758)] IN REMBANG SEA WATERS

2016 ◽  
Vol 78 (4-2) ◽  
Author(s):  
Herry Boesono ◽  
Sansan Sansan ◽  
Agus Suherman
Keyword(s):  
Field Experiment ◽  
Small Scale ◽  
Marine Resources ◽  
Homogeneity Test ◽  
Fishing Gear ◽  
Swimming Crab ◽  
Portunus Pelagicus ◽  
Anova Test ◽  
Central Java ◽  
Normality Test

Blue Swimming Crab [Portunus pelagicus (Linnaeus,1758)] is one of the fishery commodities which is prospective to be exported, highly nutritious, commercial, and wholly valuable. In Kabongan lor village, Rembang, Central Java, Indonesia the fishing gear used to catch crabs is folded trap. Kabongan lor village has a small scale of captured marine resources, so it needs to be utilized further. Because of its large potency, a research need to be conducted to improve the haul. Different trap construction and type of baits is one important factor to improve it. This study was aimed to analyze the influence of the dome-folded traps construction and box-folded traps (control), and the influence of fermented mackerel, fresh mackerel, and puffer fish’s head as baits over the crabs in Rembang waters. The method applied is field experiment with six repetitions and two variables in a treatment. The data was analyzed using normality test, homogeneity test, and ANOVA test using SPSS program ver 20.00. The results showed that dome-folded traps gained more crabs compared to box-folded traps (control). Dome-folded traps gained 3 230 g of crabs, while box-folded traps gained 1 620 g. Meanwhile, the use of fresh mackerel as baits gained better crabs than fermented mackerel and puffer fish’s head (control). According to this research known that the captured crabs in which consume; fresh mackerel baits got 1 890 g of crabs, fermented mackerel got 1 500 g and 1 460 g for puffer fish’s head. The results of ANOVA test show the differences of folded traps construction with F sig value as much as 0.022 shows that Ho is accepted; while that with F sig value as much as 0.686 shows that the Ho is denied. Based on these results known that the different folded traps construction does affect to the amount of captured crabs while the differences of baits type do not.

scholarly journals Determination of protodioscin in tribulus terrestris by reversed-phase high-performance liquid chromatography

2021 ◽  
Vol 284 ◽  
pp. 03002
Author(s):  
Mikhail Sergeev ◽  
Georgiy Zaitsev ◽  
Dmitry Yermolin
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Raw Materials ◽  
Reversed Phase ◽  
Phosphoric Acid Solution ◽  
Biologically Active ◽  
Raw Material ◽  
Tribulus Terrestris ◽  
Column Temperature

In this study, high performance liquid chromatography (HPLC) was used to analyze the protodioscin content in Tribulus terrestris samples. They were separated by C18 chromatography column, gradient with 0.1 % phosphoric acid solution and acetonitrile was used as the mobile phase with the flow rate of 0.5 mL/min, the column temperature was 45 °C, and the detection wavelength was 203 nm. The results showed that the protodioscin content has a good linear correlation (r>0.999) within the range of 50 mg/L to 1500 mg/L. In samples of raw material from Crimea it was found: 0.546 % ± 0.013 protodioscin and in analyzed material from Western Siberia it was 0.338% ± 0.008 for n=3. Raw materials of Tribulus terrestris growing in Crimea are more promising than similar raw materials from the West Siberian region due to the higher content of protodioscin for the production of biologically active products containing steroid saponins.

Development and Validation of Two Chromatographic Methods for Simultaneous Determination and Quantification of Amiloride Hydrochloride, Hydrochlorothiazide, and Their Related Substances, in Pure and Tablet Forms

2020 ◽  
Vol 103 (3) ◽  
pp. 747-754
Author(s):  
Ibrahim A Naguib ◽  
Eglal A Abdelaleem ◽  
Fatma F Abdallah ◽  
Aml A Emam
Keyword(s):  
High Performance ◽  
Reversed Phase ◽  
Ammonia Solution ◽  
Hplc Method ◽  
Phosphoric Acid Solution ◽  
Related Substances ◽  
Rp Hplc ◽  
Significant Difference ◽  
Development And Validation ◽  
Amiloride Hydrochloride

Abstract Background Amiloride hydrochloride (AM) is a potassium sparing diuretic, while hydrochlorothiazide (HCZ) is the protype of thiazide diuretics. The combining of the studied drugs exhibits a synergistic effect. Moreover, HCZ prevents the potassium depletion side effect caused by AM. Objective Two accurate and precise simultaneous chromatographic separation methods were promoted and investigated to quantify AM, HCZ, official impurities of HCZ (cholorothiazide and salamide), and the official impurities of AM (methyl 3, 5-diamino-6-chloropyrazine-2-carboxylate). Methods The components of the quintuple mixture were quantified by two methods. The first method was high-performance thin layer chromatography (HPTLC), where exemplary separation was achieved on silica gel HPTLC F254 plates at the stationary phase using ethyl acetate–ethanol–ammonia solution (8 + 2 + 0.2, v/v) as a developing system. Scanning of bands at 273 nm was done. The second method was a reversed-phase chromatography (RP-HPLC) method using C18 (4.6 × 100 mm) column and mobile phase comprising 0.1% phosphoric acid solution–acetonitrile (90 + 10, v/v) with UV determination at 273 nm. Adjustment of the flow rate at 1 mL/min and pH at 3.6 was performed. Results Regarding RP-HPLC, optimum separation of the quintuple mixture was achieved within just five minutes. According to HPTLC, symmetric and sharp peaks were separated on the resulted chromatogram. Validity of the introduced methods was investigated by applying international conference on harmonization (ICH) guidelines. Conclusions The methods were successfully applied for assays of the studied drugs in their pure and tablet forms. No significant difference was revealed through application of statistical comparison between results of the suggested methods and those of the reported method regarding both accuracy and precision.

Development and Validation of A Reversed Phase-High Performance Liquid Chromatography Method for the Quantitative Estimation of Ramipril Drug Content from Formulated Dosage Form

2016 ◽  
Vol 6 (3) ◽  
Author(s):  
Raju Chandra ◽  
Manisha Pant ◽  
Harchan Singh ◽  
Deepak Kumar ◽  
Ashwani Sanghi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Active Ingredient ◽  
High Performance ◽  
Limit Of Detection ◽  
Quantitative Estimation ◽  
Reversed Phase ◽  
Uv Detector ◽  
Chromatography Method ◽  
Drug Content

A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the quantitative determination of Remipril drug content from marketed bulk tablets. The active ingredient of Remipril separation achieved with C18 column using the methanol water mobile phase in the ratio of 40:60 (v/v). The active ingredient of the drug content quantify with UV detector at 215 nm. The retention time of Remipril is 5.63 min. A good linearity relation (R2=0.999) was obtained between drug concentration and average peak areas. The limit of detection and limit of quantification of the instrument were calculated 0.03 and 0.09 µg/mL, respectively. The accuracy of the method validation was determined 102.72% by recoveries method.

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